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Citations for Thermo Fisher :
1751 - 1800 of 10000+ citations for Bcl2 Like Protein 14 BCL2L14 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were resolved alongside a protein ladder (PageRuler Plus Pre-stained Protein Ladder, Thermo Fisher Scientific) on NuPAGE 4-12% BisTris gradient gels using NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentration was quantified using Qubit™ Protein and Protein Broad Range (BR) Assay Kits (ThermoFisher).
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bioRxiv - Microbiology 2023Quote: ... and protein concentration was determined by protein assay (EZQ Protein Quantification Kit; Life Technologies, Carlsbad, CA). Ct226-FLAG was affinity purified using magnetic FLAG beads (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... the sections were sequentially incubated with mouse polyclonal antibody to SARS-CoV-2 N protein (1:500 dilution) and HRP-conjugated goat anti-mouse IgG secondary antibody (1:5000 dilution, Invitrogen). The sections were observed under microscope (Olympus ...
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bioRxiv - Plant Biology 2020Quote: ... proteins were detected by Coomassie Blue staining or Western blotting using anti-His6-tag antibodies (Thermo Fisher Scientific MA1-21315).
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bioRxiv - Developmental Biology 2021Quote: ... and 200 μg (HEK293T)] were incubated with anti-HA antibody coated Dynabeads Protein G for immunoprecipitation (10009D, Thermo Fisher Scientific) for 1 hour at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked in TBST with 5% milk protein and probed with the following antibodies: 1:2000 anti-Ty1 (Invitrogen), then 1:1500 goat anti-mouse IgG-HRP (Dako) ...
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bioRxiv - Molecular Biology 2020Quote: ... INS1 cells labeled with Bodipy C12 were also immunostained with mouse anti-HSP60 antibody (66-41-1-lg, Protein Tech) and Alexa Fluor 488 goat anti-Mouse IgG (A11017, Invitrogen) both at 1:300 ...
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bioRxiv - Molecular Biology 2020Quote: ... the same procedures were followed but a minimum of 400μg of lysate was used for overnight IP with 4μg of the corresponding antibody and pre-washed Pierce™ Protein A/G Magnetic Beads (88802, ThermoFisher) were added for 1h at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... 50 μL of washed (three times in reaction buffer) protein G-Dynabeads magnetic beads were added to the solution of RNA and J2 antibody (ThermoFisher) and then incubated for 4 hours at 4 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... FLAG-TEV-Halo-Ago2 expressed in S2 cells were immunopurified by an anti-FLAG antibody conjugated onto Dynabeads Protein G (Invitrogen). The beads were washed three times by wash buffer ...
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Inositol depletion regulates phospholipid metabolism and activates stress signaling in HEK293T cellsbioRxiv - Cell Biology 2022Quote: ... Protein detection was done using a custom antibody against MIPS (1:10,000) and anti-actin (1:10,000; Invitrogen MA1-744). The custom MIPS antibodies were produced by YenZym Antibodies ...
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bioRxiv - Genomics 2019Quote: ... protease and phosphatase inhibitors) and incubated with 40 μl of antibody bound to 150 μl protein A/G mixed Dynabeads (Invitrogen) and incubated overnight at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... The efficiency of the expression and purification of recombinant proteins were evaluated by 12% SDS-PAGE and Western blotting by using anti-his tag antibodies (1:3,000) (Thermo Scientific) and HRP-conjugated goat anti-mouse IgG (1:10,000 ...
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bioRxiv - Genomics 2020Quote: ... Chromatin-antibody complexes were captured for at least 3 hr using a mix of Protein A and G Dynabeads (Invitrogen). The captured immunoprecipitated complex were washed 10 min each with sonication buffer (50 mM HEPES ...
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bioRxiv - Plant Biology 2019Quote: ... The respective antibodies or IgG were coupled for 4-6 hour to Protein G Dynabeads (50µl, 10004D, Thermo Fisher Scientific) and subsequently incubated overnight with equal amounts of sonicated chromatin ...
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bioRxiv - Immunology 2019Quote: ... Pre-cleared lysates were immunoprecipitated using anti-lamin B1 antibody and these immunoprecipitated samples were incubated with protein A-sepharose beads (Invitrogen) for 2h at 4°C ...
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bioRxiv - Genomics 2020Quote: ... We also validated protein expression and inducibility of the KRABINER rescue transgenes via western blot (mouse anti-myc antibody ThermoFisher #MA1-21316 ...
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bioRxiv - Physiology 2021Quote: ... Crosslinking of Y-3 antibody and Protein A+G was done using sulfosuccinimidyl (4-iodoacetyl)aminobenzoate (Sulfo-SIAB, ThermoFisher 22327). For 1 mL of protein A+G agarose (ThermoFisher/Pierce #20421) ...
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bioRxiv - Genetics 2020Quote: ... 10 cycles). Immunoprecipitation was performed using polyclonal rabbit anti-Mcd1p (a gift from V. Guacci) antibodies and protein A Dynabeads (Invitrogen).
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bioRxiv - Microbiology 2021Quote: ... the sections were incubated with house-made mouse anti-SARS-CoV-2 nucleocapsid protein serum (1:5000) and HRP465 conjugated goat anti-mouse IgG secondary antibody (1:5000 dilution, Invitrogen). The lung sections from the vector-transduced mouse were used as negative control ...
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bioRxiv - Bioengineering 2021Quote: ... Immunostaining of BBB constructs for tight junction protein ZO-1 was conducted using ZO-1 mouse monoclonal antibody conjugated with Alexa 488 (ThermoFisher). Hydrogels were removed from the transwell insert using a biopsy punch ...
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Mechanisms of Coupling between Angiotensin Converting Enzyme 2 and Nicotinic Acetylcholine ReceptorsbioRxiv - Neuroscience 2021Quote: ... 10 μg of a polyclonal antibody to the α7 nAChR (Alomone Labs # ANC-007, Jerusalem, Israel) was conjugated to Protein G Dynabeads (Invitrogen) by mixing at room temperature (23-25 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... 200-500 μg of nuclear lysates were incubated with 2 μg of specific antibodies and 50 μg of Dynabeads Protein G (Invitrogen). After overnight incubation on a rotator at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Each protein of interest was then detected with HRP-conjugated goat anti-rabbit or anti-mouse IgG antibody (1:2000; Invitrogen), and visualized using SuperSignal West Pico PLUS Chemiluminescent Substrate or SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The MERS-CoV viral lysate was immuno-detected with a MERS-coronavirus spike protein S2 polyclonal antibody (Invitrogen, Carlsbad, CA) followed by a donkey anti-rabbit IgG (H+L) ...
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bioRxiv - Physiology 2020Quote: ... antibodies were cross-linked to 200 μl of protein G-magnetic Dynabeads using 20 mM dimethyl pimelimidate (Thermo Fisher Scientific) (46) ...
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bioRxiv - Cell Biology 2021Quote: ... Protein lysates (10-15 mg, tissues) were then incubated overnight at 4 °C with antibody-coated dynabeads (Thermo Fisher Scientific). Bound immune complexes were washed twice with lysis buffer containing NP-40 ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies conjugated with Alexa Fluor fluorescent proteins (488, 568, 647) were used in dilution of 1:200 (Molecular Probes no ...
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bioRxiv - Microbiology 2021Quote: ... Proteins of interest were detected with HRP-conjugated secondary IgG antibody and visualized with ECL Western blotting substrate (Thermo Scientific) according to the provided protocol.
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bioRxiv - Microbiology 2021Quote: ... Antibodies produced from cell culture supernatants were purified immediately by affinity chromatography using recombinant Protein G-Agarose (Thermo Fisher Scientific) or CaptureSelect™ IgA Affinity Matrix (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... using 5 μgr Diagenode Ab C15410004 H3K9ac antibody and a mix of 25/25 μl of protein-A/G dynabeads (Invitrogen). 5-10 ng of DNA per sample were used for library preparation with Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... Supernatant was collected for 7 days and the antibody was purified using a protein A column (ThermoFisher Scientific, 89960; IL). The original murine anti-PD1 monoclonal antibody (63 ...
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bioRxiv - Immunology 2022Quote: ... Antibodies produced from cell culture supernatants were purified immediately by affinity chromatography using recombinant Protein G-Agarose (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Anti-CD18 antibody H52 (Developmental Studies Hybridoma Bank) was conjugated with Alexa Fluor 488 using Alexa Fluor 488 protein labeling kit (ThermoFisher) as described by manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2022Quote: ... Bio-Rad] secondary antibody and the protein was detected using Pierce™ ECL Western Blotting Substrate (32106, Thermo Fisher Scientific). Protein levels were quantified and compared using ImageJ (National Institutes of Health ...
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bioRxiv - Biochemistry 2021Quote: ... Solubilised protein was cleared by centrifugation (10 min, 125000xg, 4°C) and incubated with antibodies cross-linked to Dynabeads (Invitrogen) by overhead rotation for 2 h on ice ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 mg of pre-cleared HeLa nuclear extracts were incubated overnight at 4°C with either 40 μg ATRX antibody or rabbit IgG cross-linked to Dynabeads Protein G (Invitrogen). Beads were washed three times with 5 ml wash buffer (20 mM HEPES ...
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bioRxiv - Immunology 2021Quote: ... IF staining was performed on deparaffinized and rehydrated tissue sections using rabbit anti-SARS-CoV-2-N protein antibody together with AF568-conjugated goat anti-rabbit IgG (Invitrogen). Briefly ...
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bioRxiv - Biochemistry 2022Quote: ... The interaction between B56 subunits and PME-1 was detected by co-immunoprecipitation using anti-HA antibody immobilized on protein G magnetic beads (Invitrogen) to immunoprecipitate PME-1 bound to HA-B56 48 h after transfection ...
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bioRxiv - Molecular Biology 2019Quote: ... Lysed cells were then centrifuged at 14,000 g for 15 min at 4°C and the supernatant was added to pre-washed and antibody-coupled Protein G Dynabeads (Invitrogen). For each RIP ...
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bioRxiv - Cancer Biology 2020Quote: ... Nuclear lysates were incubated overnight at 4°C with/without the indicated antibody then immune complexes were captured by addition of protein A/G magnetic beads (ThermoFisher) for 2h at RT ...
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bioRxiv - Genetics 2019Quote: ... 550 µl of cell lysates were pre-incubated with the following antibodies for hours at 4°C prior to overnight incubation under rotation with magnetic Dynabeads-protein-G (Invitrogen): for ORC ChIP ...
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bioRxiv - Molecular Biology 2020Quote: The Ago2-IP of the TL samples were performed using 2A8 Anti-Ago monoclonal antibody tethered to Protein A Dynabeads (Invitrogen), according to the original authors (Maragkakis et al. ...
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bioRxiv - Microbiology 2020Quote: ... Cleavage of junctional proteins was detected by Western blot analysis using specific antibodies against E-cadherin (Mouse, mAb, Thermo Scientific), p120-catenin (Rabbit ...
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bioRxiv - Microbiology 2021Quote: ... For immunofluorescence analysis the cells were fixed as described above and stained with a monoclonal mouse antibody against the viral capsid proteins followed by staining with a goat anti-mouse IgG Alexa Fluor 488 (Invitrogen).
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bioRxiv - Biochemistry 2021Quote: ... the thermal unfolding temperatures of the chimeric antibodies (0.3 mg/mL) were assessed in triplicate using the Protein Thermal Shift Dye Kit (ThermoFisher Scientific). Continuous fluorescence measurements (λex = 580 nm ...
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bioRxiv - Genomics 2021Quote: ... A specific antibody or a total rabbit IgG control was added to the lysate along with Protein A magnetic beads (Invitrogen) and incubated at 4 °C overnight ...
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bioRxiv - Microbiology 2021Quote: ... Hybridoma cells producing anti-muroricin antibodies were propagated in WHEATON CELLine bioreactor 350 using protein-free hybridoma medium 1× (Gibco) for 5 to 7 days at 37°C in 5% CO2 ...
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bioRxiv - Immunology 2020Quote: ... slides were stained with the following secondary antibodies: polyclonal goat anti-Mouse Alexa Fluor488 to detect CD68 protein (Green color) (A-11029, Invitrogen) or polyclonal goat anti-Rabbit Alexa Fluor546 to detect SP140 protein (Orange color ...