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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for Bcl2 Like Protein 14 BCL2L14 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... Urea (U15-500) and uric acid (A13346-14) were purchased from Fisher Scientific (Fisher Scientific, Waltham, MA, USA). The oral phase base solution contained potassium chloride ...
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bioRxiv - Genomics 2022Quote: ... The exposed DNA ends were repaired and covalently linked with biotin-14-dCTP (Invitrogen Life Technologies, Carlsbad, CA), and then connected by T4 DNA ligase (Invitrogen Life Technologies ...
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bioRxiv - Genomics 2022Quote: ... The exposed DNA ends were repaired and covalently linked with biotin-14-dCTP (Invitrogen Life Technologies, Carlsbad, CA), and then connected by T4 DNA ligase (Invitrogen Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: Cortical neurons 10-14 DIV were treated with Neurobasal-A (NB-A) media without glucose (Gibco # A24775-01) or with media supplemented with 5-mM glucose (control condition) ...
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bioRxiv - Neuroscience 2023Quote: ... and then sectioned into 14 μm coronal sections on a cryostat sectioning machine (Thermo Fisher scientific, Microm HM525). RNA in situ hybridization was performed as previously described 46 ...
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bioRxiv - Molecular Biology 2024Quote: ... The dissociated mESC were first labeled with the eBioscienceTM Fixable Viability Dye eFluorTM 780 (ThermoFisher, 65-0865-14) to select viable cells and then stained for surface markers with indicated fluorochrome-conjugated antibodies diluted in FACS buffer (PBS 1X ...
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bioRxiv - Microbiology 2024Quote: ... Single-cell suspensions were then stained with eFluor 780-conjugated viability dye (Invitrogen eBioscience cat # 65-0865-14), and fluorescently tagged with monoclonal antibodies against CD3 (PerCP-eFluor 710 ...
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bioRxiv - Immunology 2023Quote: ... RNA was converted into cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems #43-688-14) using 2 µg RNA per 20 µl reaction ...
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bioRxiv - Genetics 2023Quote: ... Frozen tissue blocks were sectioned at 14 µm and stained with DAPI before mounting using Prolong Diamond (Invitrogen). Sections were imaged using Zeiss Axio Scan.Z1 scanner at the RNA In Situ Hybridization core (Baylor College of Medicine ...
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bioRxiv - Molecular Biology 2023Quote: ... For in vitro transcription (IVT), 7.5 µL DNA was incubated (14 h, 37°C) with IVT mix (0.5 µL Ribolock inhibitor, Invitrogen, 2 µL T7 polymerase buffer ...
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bioRxiv - Microbiology 2023Quote: ... Dm0 (DSHB, ADL67.10), α-Tubulin (Beyotime, AF0001), Flag tag (HUABIO, 0912-1) and GP64 (Invitrogen, 14-6995-85) were used.
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bioRxiv - Cancer Biology 2023Quote: ... were co-transfected with lentiviral expression construct (3 µg) using Lipofectamine 2000 (14 µl; Life Technologies, 11668-019) into HEK-293T cells to generate lentivirus ...
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bioRxiv - Developmental Biology 2023Quote: ... Transduced cells were selected for 14 days by growth in culture medium supplemented with 1µg/ml puromycin (Invitrogen).
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bioRxiv - Immunology 2023Quote: ... T98G (ATCC / CRL-1690) and U87 GBM (ATCC / HTB-14) cells were cultured with high glucose DMEM (Gibco) and %10 FBS (Gibco) ...
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bioRxiv - Neuroscience 2023Quote: ... while ‘dox (food)’ mice were switched to chow containing doxycycline 200 mg/kg (Fisher Scientific, 14-727-450) beginning two weeks after RV injection and maintained on doxycycline chow until perfusion ...
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bioRxiv - Neuroscience 2024Quote: Human CSF2RB WT (exons 11 to 14) and A455D mutant donor targeting sequences were cloned into pDONR221 (ThermoFisher) with the following layout (Supplementary Figure S1) ...
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bioRxiv - Immunology 2023Quote: ... and stained for 30mins at 4°C with: Fc blocker CD16/32 (clone 93, Invitrogen #14-0161-85), Epcam-BV421 (clone G8.8 ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were passaged and subjected to a 14-day selection with 200 μg/ml hygromycin B (ThermoFisher 10687010). The selected cell line was validated by Western blotting.
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Treg cells drive MYCN-mediated immunosuppression and tumor aggressiveness in high-risk neuroblastomabioRxiv - Cancer Biology 2023Quote: ... The frozen fish were embedded in O.C.T compound (SAKURA) and sectioned at a 14 µm thickness using a cryostat (ThermoFisher).
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bioRxiv - Developmental Biology 2023Quote: ... 20% sucrose in 0.1M PB and sectioned (12 or 14 µm) at the cryostat (CryoStar Nx70, Thermo Scientific). Cryosections were stored at -20°C.
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bioRxiv - Synthetic Biology 2024Quote: ... The cell suspension was lysed by sonication and the lysate was clarified by centrifugation at 14,000 rpm (34,000 xg) for 45 min in a F14-14 × 50cy rotor (ThermoFisher). The clarified lysate was then loaded onto a sucrose cushion with 24 mL of buffer B (20 mM Tris–HCl pH 7.5 ...
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bioRxiv - Cancer Biology 2024Quote: ... Reverse transcription PCR was performed using the High-Capacity cDNA Reverse Transcription Kit (Fisher Scientific, 43-688-14). Quantitative PCR is performed on the QuantStudio 3 using SYBR Green (Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were embedded in OCT compound in cryomolds and sectioned coronally in 14 µm sections onto SuperFrost (ThermoFisher) slides ...
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bioRxiv - Bioengineering 2024Quote: ... 20 ng/mL mouse IL-4 (M2 polarizing positive control; ThermoFisher Scientific, USA, Cat. No. 214-14-1MG), FPLC-purified PAML-31-1 at 5 x 109 PFU/mL ...
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bioRxiv - Biochemistry 2024Quote: The GPR4-Gs pH 6 complex was concentrated to 14 mg/mL supplemented with 0.05% CHAPS (Thermo Fisher) and 3 µL was applied onto a glow-discharged 300 mesh 1.2/1.3 gold grid covered in a holey gold film (UltrAufoil) ...
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bioRxiv - Microbiology 2020Quote: ... and proteins detected using specific primary (diluted at 1:1000) and HRP-secondary antibodies (ThermoFisher, diluted at 1:10,000). Protein bands were detected using Pierce SuperSignal West Pico chemiluminescent substrate kit (Pierce ...
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bioRxiv - Microbiology 2021Quote: The HA pull down was performed using 50μl of the Immobilized Recombinant Protein G Resin (Generon) and specific antibodies against HA (Invitrogen). In order to perform the pull down ...
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bioRxiv - Molecular Biology 2020Quote: ... Each half of diluted lysate is incubated with 1/10 volume of antibody coated Protein G Dynabeads™ (Invitrogen) for 40 min at 4°C by end to end rotation ...
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bioRxiv - Neuroscience 2021Quote: ... were used to bind to presynaptic axonal regions and anti-post synaptic density protein 95 (PSD-95) antibodies (Invitrogen, Carlsbad ...
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bioRxiv - Cell Biology 2022Quote: ... The endogenous antibodies were added for 4 °C overnight reaction before the incubation with protein G Dynabeads (Life Technologies) for additional 2 hrs ...
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Dual functionality of the TasA amyloid protein in Bacillus physiology and fitness on the phylloplanebioRxiv - Microbiology 2019Quote: ... the membranes were probed with anti-TasA antibody (rabbit) used at a 1:20,000 dilution in Pierce Protein-Free (TBS) blocking buffer (ThermoFisher). For immunodtection of FloT-YFP ...
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bioRxiv - Immunology 2020Quote: ... Antibody concentration was determined by measuring the amount of A280 protein using Nanodrop 2000 (Thermo Fisher Scientific, Waltham, MA). Conjugated antibodies were then diluted in PBS-based antibody stabilization solution or LowCross-Buffer (Candor Bioscience GmbH ...
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bioRxiv - Cell Biology 2019Quote: ... Anti-PRG-1 antibody (Custom) or rabbit IgG was pre-coupled to protein A/G matrix (Thermo Scientific, 88802) and incubated with the supernatant of worm lysates for 4 h (Anti-PRG-1 with N2 lysates ...
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bioRxiv - Cell Biology 2019Quote: ... The polyclonal anti-EhPKDL antibody used was raised in rabbit and subsequently purified with Protein A-Sepharose (Invitrogen, USA). Dilutions of antibody used were ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then initially stained with microtubule-associated protein 2 (MAP2) monoclonal primary antibody (M13) (ThermoFisher Scientific,ON, Canada) at a 1:300 dilution then incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Vps54/scat protein was detected using HRP secondary antibodies with the SuperSignal West Pico ECL chemiluminescent substrate (ThermoFisher 34580) and scanned on a C-DiGit blot scanner ...
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bioRxiv - Microbiology 2020Quote: ... hybridoma cells were pelleted at 1000 x g and the supernatant containing antibody P3.2 was filtered with 0.22 μm filter units (Nalgene) before being loaded onto Protein G resin (Thermo Fisher) previously equilibrated with 20 mM HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... and immunoprecipitation of the precleared lysate was performed using polyclonal anti-core antibody and protein A sepharose beads (Invitrogen). Immunoprecipitated complexes were washed twice in buffer B (1% Triton X100 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then washed with flow buffer 1X and incubated with protein A-488 secondary antibody conjugate (Invitrogen, P11047) for 20 minutes (1:1000 dilution) ...
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bioRxiv - Neuroscience 2022Quote: ... pH 7.6) and subjected to preclearing and overnight incubation with Reelin antibody G10 and Dynabeads Protein G (ThermoFisher Scientific). For silver staining of SDS-gels PierceTM Silver Stain for mass spectrometry (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... MX2-Flag proteins were detected using anti-Flag monoclonal M2 and secondary antibody conjugated to Alexa 594 (Invitrogen, A21203). Endogenous NUP358 was detected using rabbit anti-NUP358 antibody (Abcam ...
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bioRxiv - Immunology 2022Quote: ... Supernatant was collected for 7 days and the antibody was purified using a protein A column (ThermoFisher scientific, # 89960). Ligand binding affinity and sensitivity were assessed using ELISA techniques.
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bioRxiv - Immunology 2022Quote: ... Protein expression was confirmed by western blot using a 6x His-Tag HRP conjugated Monoclonal Antibody (Thermo Fisher Scientific). Once verified ...
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bioRxiv - Immunology 2020Quote: ... cells were stained with 2.5 µg of a SARS-CoV spike protein polyclonal antibody (PA1-41165, Thermo Fisher Scientific) for 30 min on ice before washing with 2.5 mL of FACS buffer and centrifuging at 1750 RPM for 7 min ...
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bioRxiv - Genomics 2022Quote: ... diluted chromatin was incubated for 2 h with antibodies (2.5 μg/100 μL) coupled to magnetic Dynabeads Protein A (Invitrogen). ChIP samples were washed 4 times in ice-cold RIPA buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... mouse monoclonal or anti-GFP rabbit polyclonal (ab290) antibody was incubated with Protein-G/A magnetic beads (Dynabeads, Invitrogen) in PBST for 2hrs at 4 °C ...
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bioRxiv - Genetics 2019Quote: ... The chromatin with the antibodies was incubated with 6 μl of Dynabeads Protein G (Thermo Fisher Scientific, Cat# 10003D) for one hour ...
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bioRxiv - Cell Biology 2019Quote: ... 5 mg protein was incubated with 125 μg α-GFP antibody covalently coupled to M270 magnetic resin (ThermoFisher Scientific) for 1 h with rotation ...
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bioRxiv - Molecular Biology 2019Quote: ... The supernatant was then incubated with specific antibodies and immune complexes were collected using Dynabeads Protein A/G (ThermoFisher). For silver staining ...
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bioRxiv - Pathology 2021Quote: ... The supernatant was moved to a clean tube and incubated overnight with 0.5 μg of either goat anti-mouse KIM-1 or mouse anti-MUC1 (B27.29) antibodies and Protein G conjugated to Sepharose 4B (ThermoFisher). Beads were washed with 0.5 ml HEPES-buffered saline (HBS ...