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Citations for Thermo Fisher :
1951 - 2000 of 10000+ citations for Bcl2 Like Protein 14 BCL2L14 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... cells were collected and stained with fixable viability dye eFluor780 (1:2000 dilution Life Technologies, catalog no. 65-0865-14;) followed by surface staining for PDL1 (CD274 ...
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bioRxiv - Neuroscience 2022Quote: ... Rat: anti-SOX2 (Thermo, 14-9811-82 1:500) Other: Streptavidin-Alexa Fluor 594 conjugate (Thermo Scientific, S11227, 1:500).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 14 μm sections of retinal explant cultures from the center of the tissue were obtained using a NX50 cryostat (Thermofisher). The sections were hydrated with PBS for 10 min ...
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bioRxiv - Biophysics 2022Quote: ... 0.27 mM L-asparagine, 14 μM folic acid, 1x Corning Penicillin/Streptomycin (100 IU, 0.1 mg/mL respectively) (Fisher Scientific), 50 μM β-mercaptoethanol.
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bioRxiv - Biophysics 2024Quote: ... γδ T cells were expanded from freshly prepared PBMCs for 14 days as follows: the PBMCs were cultured at 2x106 cells/mL in AIM-V medium (ThermoFisher) supplemented with 10 % heat-inactivated human serum from human male AB plasma (Sigma-Aldrich) ...
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bioRxiv - Immunology 2024Quote: ... sections were blocked with 5% mouse serum and labelled using anti-mouse CD8 (clone 4SM15, Invitrogen cat 14-0808-82) followed by anti-rat IgG Alexa555 ...
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bioRxiv - Cell Biology 2024Quote: ... Heterozygous HeLa TFG-mClover-FLAG cells were seeded on glass-bottom Mattek dishes (P35GC-1.5-14-C) for live cell imaging with live imaging solution (Invitrogen A1429IDJ) supplemented with 10 mM final concentration of glucose (Sigma G8644-100ML) ...
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bioRxiv - Immunology 2024Quote: ... 14- 4301-85), and anti-IFNAR1 (mouse IgG1κ, 16-5945-85), its isotype control (mouse IgG1κ, 14- 4714-85) were from Invitrogen. Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Immunology 2024Quote: ... the membranes were probed overnight at 4°C with anti-mouse CASP-1 (Cat# 14-9832-82; Thermo-Fisher Scientific) or anti-mouse mature IL-1β (Cat# 63124S ...
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bioRxiv - Immunology 2023Quote: ... each fraction (fraction 1-14) was collected separately and incubated with 20mg/ml Proteinase K (Thermo Scientific 25530049, 1:100), 0.5M EDTA and 20% SDS for 30 minutes at 42°C ...
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bioRxiv - Molecular Biology 2022Quote: ... An overnight (14 hour) capillary transfer setup was used to transfer the DNA to BrightStarTM – Plus positively charged nylon membrane (Invitrogen) using 20X SSC transfer buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Total RNA was extracted from mouse spinal cord segments (∼2 mm) rostral to the lesion epicenter 14 days after injury using TRIzol reagent (ThermoFisher) following the manufacturer’s instructions and methods previously described (21) ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were incubated overnight at 4ºC with mouse anti-human αSMA (Cat. 14-9760-82, Invitrogen: 1:500 dilution) and goat anti-human CD144 (Cat ...
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bioRxiv - Cancer Biology 2023Quote: ... and then selected for 14 days in puromycin (2 µg/mL for ID8 and 8ug/mL for UPK10; ThermoFisher Scientific). Transduction efficiency was confirmed by quantitative reverse transcription polymerase chain reaction (qRT-PCR ...
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bioRxiv - Cell Biology 2023Quote: ... then normalized RNA was transformed into cDNA using the High-capacity cDNA reverse transcription kit (43-688-14, Applied Biosystems). QPCR was performed on a 7900HT fast real-time PCR system (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... Explants were embedded in Tissue-Tek (Sakura Finetek Europe B.V., Alphen aan den Rijn, The Netherlands) and sectioned (14 µm) in a cryostat (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: Kuutsalo-14 data were collected at the SciLifeLab CryoEM Infrastructure Unit (Solna, Sweden) using FEI Titan Krios microscope (Thermo Fisher) operating at 300 kV equipped with a Gatan K3 detector in counting mode at nominal magnification 81,000 x resulting in a sampling rate of 1.075 Å/px ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were incubated for 4 hours at 23°C with the Klenow DNA polymerase I to fill in the 5’ overhang and incorporate biotin-14-dATP followed by chromatin ligation for 4 hours at 16°C with T4 DNA ligase (Invitrogen). Then cells were left overnight in 400 ng/ml proteinase K and 0.5% SDS at 60°C overnight to reverse cross-linking ...
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bioRxiv - Immunology 2023Quote: ... and 14 cycles of PCR amplification using the Ion Xpress™ RNA-Seq Barcode 1-16 Kit (Thermo Fisher Scientific). Yield distribution of the libraries was measured with the Qubit 1x dsDNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... qPCR primers were designed using the GenScript RT-PCR primer design tool and a standard reference plasmid was generated by amplifying genomic DNA from K562 cells with each primer pair followed by TA cloning the amplicon into a pCR2.1 vector (Supplementary Table 14) using a TA cloning kit (451641; Thermo Fisher). TA cloning was conducted using the recommended protocol and plasmids were transformed into TOP10 cells (C404003 ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were plated 24 hours before transfection in a cell-culture treated 6-well plate (Fisher Scientific, 14-832-11). To transfect ...
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bioRxiv - Immunology 2023Quote: ... Cryosections of 14 μm thickness were generated using a cryostat (Microm Cryo-Star HM560, Thermo Fisher Scientific, Waltham, MA, USA). The sections were fixed for 1 hour with 4% paraformaldehyde (PFA ...
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bioRxiv - Immunology 2023Quote: ... Dead cells were excluded by staining with Viability-Dye (conjugated with eFluor 506, cat#65-0866-14, Thermo Fisher Scientific). The antibody staining was permitted for 30min at 4°C.
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bioRxiv - Immunology 2023Quote: ... 4mM EDTA and stained for 30mins at 4°C with: Fc blocker CD16/32 (clone 93, Invitrogen #14-0161-85), CD4-AF488 (clone GK1.5 ...
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bioRxiv - Neuroscience 2023Quote: ... Spinal cords were collected from postnatal 7–14 day old mice and placed in Hanks balance buffer solution (Gibco; UK) on ice ...
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bioRxiv - Bioengineering 2023Quote: ... PDMS rods were fabricated from 23-gauge hypodermic needles (inner diameter -230 μm, 14-840-88, Fisher scientific, Pittsburgh, PA), extracted from the needles ...
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bioRxiv - Neuroscience 2023Quote: DIV 12-14 hippocampal neurons were transfected with plasmid DNA and/or miRDIAN miRNA inhibitors (Horizon) using Lipofectamine 2000 (Invitrogen) and used for experiments at DIV14-16.
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bioRxiv - Cancer Biology 2024Quote: The remainder of the lysates were then transferred to 13×100mm borosilicate glass culture tubes (Fisher Scientific, #14-961-27). Lysates were acidified with 10ul of 1N HCl (prewashed with Hexanes ...
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The Iroquois (Iro/Irx) homeobox genes are conserved Hox targets involved in motor neuron developmentbioRxiv - Developmental Biology 2024Quote: Motor neurons derived from the HUES3 HB9::GFP line were plated on day 14 on top of 1.5mm glass coverslips (Neuvitro, Fisher scientific) coated with PDL (0.1mg/ml ...
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bioRxiv - Plant Biology 2024Quote: ... 500 ng of total genomic DNA were labelled by random priming with biotin-14-dCTP (Invitrogen by Thermo Fisher Scientific) used as probes.
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bioRxiv - Plant Biology 2024Quote: ... 500 ng of total genomic DNA were labelled by random priming with biotin-14-dCTP (Invitrogen by Thermo Fisher Scientific) used as probes.
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bioRxiv - Molecular Biology 2020Quote: ... We used 1.6 μg of c-Myc monoclonal antibody and 50 μL PanMouse IgG magnetic beads or 1 µl of anti-Flag antibody and 30 µl Protein G magnetic beads (Thermo Scientific). For Cdc5-TAP ChIP ...
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bioRxiv - Molecular Biology 2020Quote: ... Precleared nuclear protein lysates (500 μg) were incubated with 20 μl of anti-c-MYC tag antibody coupled to magnetic beads (Thermo Scientific). After two hours ...
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bioRxiv - Immunology 2021Quote: ... and IP was performed with antibodies against survivin (RnD AF886) coupled to the Dynabeads Protein G Immunoprecipitation Kit (10007D, ThermoFisher Scientific), cross-linked with bis(sulfosuccinimidyl)suberate (A39266 ...
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bioRxiv - Microbiology 2021Quote: ... A semi-dry transfer method was used to transfer proteins to nitrocellulose for western blotting using anti-GFP antibodies (Life technologies) at a 1:5000 dilution ...
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bioRxiv - Molecular Biology 2020Quote: ... the lysate volume was increased to 1 ml and 8 μg of antibody were used in combination with Protein G Dynabeads M-280 (Thermo Scientific) as described above ...
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bioRxiv - Cancer Biology 2019Quote: ... HRP-conjugated secondary antibodies (31430 and 31460) and Dynabeads™ Protein G (10004D) were obtained from Life Technologies (Carlsbad, California, USA).
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bioRxiv - Genetics 2019Quote: ... Chromatin-antibody complexes were recovered using pre-blocked (1mg/ml BSA and 1ug/ml salmon sperm DNA) Dynabeads Protein A (Life technologies). Purified DNA was analysed by quantitative PCR using Roche Lightcycler480 using SYBR green reagents (Roche) ...
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bioRxiv - Molecular Biology 2019Quote: ... A peroxidase-conjugated antibody was used to reveal the proteins of interest with the Pierce ECL Western blotting substrate (ThermoFisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... Ribosomes were immunoprecipitated using anti-EGFP antibodies (an equal mixture of clones 19C8 and 19F7 from Monoclonal Antibody Core Facility at Memorial Sloan-Kettering cancer center) that were previously conjugated to Protein L coated magnetic beads (ThermoFisher Scientific) for 16-h in 4°C with rotating ...
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bioRxiv - Bioengineering 2020Quote: Each gel electrophoresis reaction used 6 µL of unbound B56 protein-antibody complex and 2 µL of 4x SDS-PAGE sample buffer (Thermo Fisher). After heating the samples to 95 C for 10 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... 0.5-1.0 mg of cell lysate was incubated with 2-4 µg of antibody for 1 h followed by incubation with 8-10 µl of Protein G agarose (Thermo Fisher) for another 30-45 min ...
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bioRxiv - Neuroscience 2021Quote: ... 10 μg of primary antibodies or isotype control IgG were covalently cross-linked to 25 μl of protein A/G magnetic beads (#88802, Thermo Fisher). Proteins were extracted with the Pierce IP Lysis/Wash Buffer (#87787 ...
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bioRxiv - Microbiology 2020Quote: ... The dengue 2 E protein antibody PA5-32246 was used at 1:10,000 for western blotting (Thermo-Fisher Scientific, Waltham, MA). Secondary detection for western blotting used anti-rabbit HRP antibody diluted 1:10,000 (Amersham ECL ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitated chromatin with anti-HA antibody or IgG was then incubated with Dynabeads Protein G (Thermo Fisher Scientific, cat. no. 10003D) for 2 h ...
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bioRxiv - Developmental Biology 2021Quote: ... The target proteins were visualized with the secondary antibodies conjugated with fluorescence (Alexa fluor 488 and 594, 1:400; Invitrogen, CA) and Hoechst nuclear stain ...
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bioRxiv - Molecular Biology 2021Quote: ... blots were incubated with HRP conjugated secondary antibody and proteins were imaged by chemical luminescence using Super Signal West Pico (Thermo Scientific).
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bioRxiv - Molecular Biology 2022Quote: ... The supernatants of lysates were mixed with anti-c-Myc antibody (Nacalai tesque) attached to Protein G DynabeadsTM (Thermo Fisher, 10004D). After incubation for 1 hr ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 µg of an HA-tag antibody (MBL, M132-3) was mixed with 20 µL of Dynabeads Protein G (Invitrogen, #10004D) and subsequently added to extracts ...
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bioRxiv - Microbiology 2022Quote: ... GLUT4 protein was used as a housekeeping expression control and labeled using a GLUT4 monoclonal antibody (Cat: MA5-17175, Invitrogen Co.).