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Citations for Thermo Fisher :
1701 - 1750 of 10000+ citations for tert Butyl 10 oxo 4 9 diazaspiro 4.5 decane 4 carboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Samples were heated for 10 min at 100°C and loaded onto a 1.5 mm NuPAGE gradient (4-12%) gel (Invitrogen, NP0336BOX). After electrophoresis ...
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bioRxiv - Immunology 2023Quote: ... penicillin/streptomycin (10 μg/mL) and supplemented with the cytokines IL-4 (500 U/mL) and GMCSF (800 U/mL) (both Gibco) [42] ...
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bioRxiv - Microbiology 2023Quote: Four milliliters of each resuspension were pelleted via centrifugation (14,000 g, 10 min, 4 °C) (Heraeus Fresco 21, Thermo Scientific), the cells washed 4 times with ice-cold 0.9% (w/v ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated on precast Novex 10% Tris-Glycine gel / NuPAGE 4-12% Bis-Tris gel at 100V using the Mini Gel Tank (Invitrogen) and were blotted onto PVDF membrane at 20V for 90 min ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein samples were resolved on 4-12% NuPAGE Bis-Tris gel with protein molecular weight standards (PageRuler Prestained Protein Ladder, 10 to 180 kDa, Thermofisher). The gels were transferred onto PVDF membranes using the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... were then heated at 95°C for 10 min and resolved on pre-cast 4-12% SDS-PAGE gradient gels (Invitrogen). Transfer of proteins from SDS-PAGE gels to nitrocellulose membranes was performed by dry transfer using the iBlot™ 2 Gel Transfer Device (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell debris were removed by 12000 rpm centrifuge for 10 min in 4°C and supernatants were mixed with anti-DYKDDDDK magnetic agarose (Cat# A36797, ThermoFisher) in 4°C overnight on rotating mixer according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Pancreata/tumors were minced and digested at 37°C for 30 minutes at 150 rpm in a 10 mL mixture of 4 mg/mL collagenase IV (Gibco) and 4 mg/mL dispase (Gibco) ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were boiled for 5 min and subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) on either 10% gels (cast freshly) or 4-20% gradient gels (Invitrogen). Proteins were transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 100mM DTT and were heated at 95°C for 10 min prior to resolving on a 4-12% Bis-Tris NuPage NOVEX gradient gel (Invitrogen) in 1x Novex NuPAGE MOPS SDS Running Buffer (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... and 20μg of total protein was loaded into each well of a 10-20% Tris-Glycine gel or a 4-12% Bis-Tris gel (Invitrogen). Protein was transferred to a PVDF membrane (Millipore) ...
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bioRxiv - Cell Biology 2023Quote: ... The next day each well was transfected with 100 ng pTI-ARE-LUC using FuGENE HD (4 μL per ug DNA) in freshly filtered 10 μL OptiMEM medium (Gibco), or 50 ng of the reporter and 50 ng of shRNA ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were boiled at 70 °C for 10 minutes and loaded into 4 – 12 % Bis-Tris SDS-PAGE gels (ThermoFisher) at 10 – 20 µg protein / well ...
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bioRxiv - Cell Biology 2023Quote: ... 10% of the sample was separated on a NuPAGE™ 4-12% gradient Bis-Tris SDS-PAGE gel (Thermo Fisher) in MOPS running buffer (50 mM Tris ...
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bioRxiv - Neuroscience 2023Quote: ... The ACSA2+ astrocytes were eluted from the magnetic columns and pelleted by centrifugation at 300 x g for 10 min at 4 °C and resuspended in RNA Later (Invitrogen).
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Identification of resistance mechanisms to small-molecule inhibition of TEAD-regulated transcriptionbioRxiv - Cancer Biology 2023Quote: ... at 4⁰C and centrifuged (18,000 x g and 4⁰C) for 10 minutes prior to protein quantification by BCA assay (ThermoFisher Scientific). Proteins in lysates (1µg/ml ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were boiled at 95°C and 10 µL were then run on a 4–12% Bis-Tris NuPAGE gel (Invitrogen). Bands were visualized by staining with InstantBlue Coomassie Stain (Abcam ...
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bioRxiv - Systems Biology 2023Quote: Samples were then heated at 100°C for 10 min and loaded onto a 1.5 mm NuPAGE 4-12% gradient gel (Invitrogen, NP0336BOX). After electrophoresis ...
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bioRxiv - Neuroscience 2023Quote: ... The slices were washed 4 x 10 min in 1x PBS and mounted on microscope slides and cover slipped with Fluormount-g (Invitrogen).
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bioRxiv - Microbiology 2023Quote: ... Bacteria were centrifuged for 10 min at 10,000 x g (4°C) and the cells resuspended in 0.5 ml of TRIzol (Thermo Fisher Scientific). Direct-zol ...
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bioRxiv - Biochemistry 2023Quote: ... and 10% 2-Mercaptoethanol or 50 mM dithiothreitol (DTT) and run on NuPAGE 4-12% bis-tris gels (Thermo Fisher). Proteins were transferred to nitrocellulose membranes ...
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bioRxiv - Immunology 2023Quote: ... 10/20µg of protein sample was loaded in the wells of precasted 4-12% polyacrylamide NuPAGE Novex gel (Invitrogen, NP0322BOX) submerged in 1X MOPs running buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... and samples were boiled at 95°C for 10 minutes and an equal amount of protein lysate (10-20 ug) was electrophoresed through 4–12% SDS-PAGE gels (Invitrogen) and then transferred to PVDF membranes ...
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bioRxiv - Bioengineering 2023Quote: ... incubated at 85°C for 10 min and loaded on Bolt 4-12% Bis-Tris polyacrylamide gels (Thermo Fisher Scientific). Proteins were electrotransferred to Immobilon-FL polyvinylidene difluoride (PVDF ...
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bioRxiv - Pathology 2023Quote: ... The collected cells were pelleted at 1,500 rpm for 10 min at 4 °C and resuspended in phosphate-buffered saline (PBS; Life Technologies), supplemented with 0.04% BSA (Cell suspension A).
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bioRxiv - Cell Biology 2023Quote: ... The lysate was homogenised using a sonicator (10 pulses, five seconds each) and run on NuPAGE 4-12% Bis-Tris protein gels (ThermoFisher). The iBlot 2 Dry Blotting System (ThermoFisher ...
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bioRxiv - Developmental Biology 2023Quote: Worms were relaxed in 7.14% MgCl2 in ddH2O for 10 min and fixed in 4% PFA (Thermo Scientific® - #28908) in concentrated PBS (0.1 M Sodium Phosphate pH 7.5 ...
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bioRxiv - Neuroscience 2024Quote: Electrophoresis was performed from 10-20μg of each sample in 4-12% acrylamide- bisacrylamide gradient gels (Invitrogen, Cat. No. NP0322BOX), transferring the samples to nitrocellulose membranes using the iBlot2 dry transfer system (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... prior to intra-muscular injection of Ketamine (> 100 mg/kg) and Xylazine (> 10 mg/kg) and euthanised by transcranial perfusion of 4% paraformaldehyde (PFA; Thermofisher). Fixed whole brains were removed ...
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bioRxiv - Cancer Biology 2024Quote: ... Target cells were infected with lentivirus for 4-6 hours before selection in Dulbecco’s modified Eagle’s medium + 10% fetal bovine serum (Thermo Fisher Scientific) and 1% penicillin-streptomycin (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... blocked with 2% BSA in a PBS buffer for 10 min and incubated with 0.1–1 μg/ml DAPI (4’, 6-diamidyno-2-fenyloindol, Molecular Probes) and WGA-Texas Red (Wheat Germ Agglutinin-Texas Red ...
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bioRxiv - Molecular Biology 2024Quote: ASOs were removed from 4 µl of DMS-modified RNA in 10 µl containing 1 µl of TURBO DNase (Invitrogen) and 1X TURBO DNase Buffer (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins (15-30 µg) were resolved on a 4-15% or 10% acrylamide gel and transferred onto a PVDF membrane (Invitrogen iBlot 2 or Bio-Rad Trans-Blot Turbo system) ...
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bioRxiv - Neuroscience 2024Quote: ... microglia were plated on 25 mm coverslips coated with PLL (0.01%) and Collagen IV (4 µg/ml) and loaded with 10 µM Fura-2 AM (Thermo Fisher) with 0.02% Pluronic F-127 (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... and then 35 μL of each sample was subjected to SDS-PAGE on a 10-well 4-12% gradient NuPAGE (Invitrogen) for 10 min at 150 V with MES buffer ...
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bioRxiv - Biochemistry 2024Quote: ... Cytosolic and nuclear extracts and acid-extracted histones were separated on 10% or 4-12% Bis-Tris gels (Life Technologies) and transferred to nitrocellulose membranes (0.2 μm ...
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bioRxiv - Systems Biology 2024Quote: ... Lysates were centrifuged for 10 min at 14.000 rpm and 4°C followed by protein determination with PierceTM BCA Protein Assay Kit (Thermo Scientific) according to manufacture description ...
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bioRxiv - Evolutionary Biology 2024Quote: Samples containing 1 μg total protein were diluted in 1 x loading dye with 0.1 M dithiothreitol and incubated at 70 °C with shaking at 1000 rpm for 10 mins and then separated by electrophoresis on a 4-12% NuPAGE Bis-Tris gel (Invitrogen) at 120 V for 1 h 35 mins ...
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bioRxiv - Evolutionary Biology 2024Quote: Samples containing 1 μg total protein were diluted in 1 x loading dye with 0.1 M dithiothreitol and incubated at 70 °C with shaking at 1000 rpm for 10 mins and then separated by electrophoresis on a 4-12% NuPAGE Bis-Tris gel (Invitrogen) at 120 V for 1 h 40 mins ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were precipitated at 5000g for 20 min at 4°C and resuspended in 10% fetal bovine serum (FBS, Gibco)-DMEM ...
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bioRxiv - Microbiology 2024Quote: ... Vero E6 cells (1x106 cells per 6-well) were transfected with 4 μg of BAC construct (Nsp15WT, Nsp15H234A, and Nsp15N277A) using 10 uL of Lipofectamine 2000 (Invitrogen). Next day ...
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bioRxiv - Neuroscience 2024Quote: ... 10-20 ug of protein lysates/well were separated on 4-12% polyacrylamide gels (Thermo Fisher Scientific, MA, United States) at 100 V for 60 minutes and transferred to a Nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were removed from the cryostat and immediately immersed in cold (4°C) 10% formalin (Fisher Scientific; Cat. 23-245684) for 15 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... Cell lysates from each sample were prepared and electrophoresed on a 10-well 4–12% NuPAGE gradient gel (Invitrogen, NP0321BOX) and transferred onto 0.45 μm pore polyvinylidene difluoride membranes (PVDF ...
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bioRxiv - Immunology 2024Quote: ... were heated at 95 °C for 10 min and then separated by SDS-PAGE using a NuPAGE 4-12% Bis-Tris Gel (Invitrogen). Proteins were transferred onto nitrocellulose membranes using a semi-dry transfer system (BioRad) ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were centrifuged at 16,000rcf for 10 min at 4°C and supernatant was quantified using Pierce Protein BCA assay kit (ThermoFisher Scientific). Samples were diluted in 1X NuPAGE LDS buffer (Invitrogen)/ 2.5% β-Mercaptoethanol and boiled at 70°C for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... Cell pellets were lysed in 2X Laemmli buffer and 10% of this sample was resolved using Nu-Page 4-12% Bis-Tris gels (Invitrogen) and transferred to nitrocellulose membranes using the iBlot2 system (Invitrogen ...
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bioRxiv - Physiology 2024Quote: PDMS membranes subjected to stretch (0, 10, 20%) were fixed in 4% formaldehyde and stained with Alexa Fluor 488 Phalloidin (ThermoFisher) to visualize actin ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were washed once in PBS and fixed for 10 min on ice with 4% PFA prior to mounting in Prolong gold antifade reagent with DAPI (Invitrogen). Representative maximum intensity projections (2D renderings ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were washed once in PBS and fixed for 10 min on ice with 4% PFA prior to mounting in Prolong gold antifade reagent (Invitrogen). Each condition was performed in quadruplicates and five maximum intensity projections (2D renderings ...