Labshake search
Citations for Thermo Fisher :
1651 - 1700 of 10000+ citations for tert Butyl 10 oxo 4 9 diazaspiro 4.5 decane 4 carboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were fixed in 4% paraformaldehyde for 10 min to stop the reaction and mounted with ProLong Diamond Antifade Mountant containing DAPI (Invitrogen). Imaging was performed on a BZ-X800 microscope (Keyence) ...
-
bioRxiv - Molecular Biology 2021Quote: ... 10 to 20 μg total protein was separated on a NuPAGE 4-12% Bis-Tris denaturing gel (Thermo Fisher Scientific) and transferred to a nitrocellulose membrane using an iBLot2 dry transfer (Invitrogen) ...
-
bioRxiv - Microbiology 2020Quote: ... Lysate was centrifuged for 10 min at 10,000 × g at 4°C and protein concentration in the supernatant measured by BCA assay (Thermo scientific). Lysate equivalent to 5 mg of total protein was incubated with 30 μl anti-myc magnetic beads (Thermo scientific ...
-
bioRxiv - Molecular Biology 2021Quote: ... Lysates were obtained by centrifugation at 12000 g for 10 min at 4°C and incubated with goat-anti-Flag agarose beads (PA1-32374, Invitrogen) or proinsulin Ab (CCI-17 ...
-
bioRxiv - Neuroscience 2020Quote: ... 5% DTT) for 10 min at 70 °C before being loaded on a 4-12% Bis-tris gel (ThermoFisher NP0322BOX) at 10 µg/lane ...
-
bioRxiv - Neuroscience 2020Quote: ... Homogenates were centrifuged at 14,000 rpm for 10 min 4°C and supernatants were collected for BCA assaying (ThermoFisher Scientific) to determine protein concentrations ...
-
bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of protein (10-30 μg) were separated on 4-12% Bolt bis-tris plus gels (Thermo Fisher Scientific) and transferred to Immobilon FL PVDF membranes (Millipore Sigma) ...
-
bioRxiv - Biochemistry 2021Quote: ... and the samples were boiled for 10 minutes before being separated by electrophoresis using 4 – 12% NuPAGE Bis-Tris gel (Invitrogen). The proteins were then transferred into a PVDF membrane (iBlot 2 Transfer Stacks ...
-
bioRxiv - Biochemistry 2021Quote: ... 10-30 µg of samples were loaded onto NuPAGE 4–12% Bis–Tris Midi Gel (Thermo Fisher Scientific, Cat# WG1403BOX) and electrophoresed at 130 V for 2 h with the NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2021Quote: ... Insoluble material was clarified for 10 min at 4°C at 16k rcf and incubated with 60 μl Dynabeads HIS-Tag isolation (10104D, Invitrogen) for 2 hour with rotation at 4°C ...
-
bioRxiv - Cancer Biology 2021Quote: ... Chromatin was disrupted by sonication, cleared by centrifugation (20,000 RCF, 10 minutes, 4°C) and lysates transferred to cryovials (ThermoFisher 374081) and stored in vapor phase of an LN2 tank ...
-
bioRxiv - Immunology 2021Quote: ... Equal amounts of protein were electrophoresed on either 4-12% Bis-Tris or 10% Bis-Tris gels (NuPAGE, Life Technologies) and wet-transferred to nitrocellulose membranes ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples were heated at 95 °Cfor 10 min and separated on 4–20% acrylamide Tris-glycine gradient gels (Life Technologies). Proteins were transferred to PVDF membranes (EMD Millipore ...
-
bioRxiv - Molecular Biology 2021Quote: ... Beads eluate was heated at 95°C for 10 min and loaded on polyacrylamide gel (4-12% Bis-Tris gel (Invitrogen)) and run in MOPS SDS Running Buffer (Life Technologies) ...
-
bioRxiv - Cancer Biology 2020Quote: ... All cell lines were maintained in DMEM (25 mM Glucose, 4 mM Glutamine, 1 mM Sodium Pyruvate) supplemented with 10% Fetal Bovine Serum (FBS, Gibco) and 50 units/mL Penicillin/Streptomycin (Gibco ...
-
bioRxiv - Cell Biology 2023Quote: ... Transfected cells were incubated with a total of 4 µg DNA and 10 µL Lipofectamine 2000 (11668019, Thermo Fisher Scientific) complex for 72 hours and selected for another 14 days with 300µg/mL Zeocin ...
-
bioRxiv - Immunology 2023Quote: ... added with 50 mM DTT for 10 min at 75 C and loaded onto precast NuPAGETM 4-12% bis-tris protein gels (Invitrogen), or NuPAGETM 12% bis-tris protein gels (these ones were used for LC3 immunoblotting ...
-
bioRxiv - Molecular Biology 2023Quote: ... 10 µl of the eluted proteins were separated by migration on a NuPAGE 4–12% Bis- Tris Protein Gel (Invitrogen) and silver stained ...
-
bioRxiv - Molecular Biology 2023Quote: ... Slides were then washed 4× for 5 min in PBS and either mounted with Vectashield with DAPI (Vector, H-1200-10) or fixed in 4% PFA (Thermofisher) in PBS for 20 min at room temperature to be processed for immuno-FISH staining.
-
bioRxiv - Cell Biology 2022Quote: ... pMDG2.1-VSV-G and p8.91-GAG-POL in a ratio of 4:1:2 to a total of 10 μg of DNA using Lipofectamine 2000 (ThermoFisher) as per the manufacturer’s instructions overnight ...
-
bioRxiv - Systems Biology 2023Quote: Samples were then heated at 100°C for 10 min and loaded onto a 1.5 mm NuPAGE 4-12% gradient gel (Invitrogen, NP0336BOX). After electrophoresis ...
-
bioRxiv - Neuroscience 2023Quote: ... The slices were washed 4 x 10 min in 1x PBS and mounted on microscope slides and cover slipped with Fluormount-g (Invitrogen).
-
bioRxiv - Cancer Biology 2023Quote: ... Samples were heated for 10 min at 100°C and loaded onto a 1.5 mm NuPAGE gradient (4-12%) gel (Invitrogen, NP0336BOX). After electrophoresis ...
-
bioRxiv - Immunology 2023Quote: ... penicillin/streptomycin (10 μg/mL) and supplemented with the cytokines IL-4 (500 U/mL) and GMCSF (800 U/mL) (both Gibco) [42] ...
-
bioRxiv - Cell Biology 2023Quote: ... The lysate was homogenised using a sonicator (10 pulses, five seconds each) and run on NuPAGE 4-12% Bis-Tris protein gels (ThermoFisher). The iBlot 2 Dry Blotting System (ThermoFisher ...
-
bioRxiv - Cancer Biology 2023Quote: ... Samples were then heated at 100°C for 10 min and loaded onto a 1.5 mm NuPAGE gradient (4-12%) gel (Invitrogen, NP0336BOX). After electrophoresis ...
-
bioRxiv - Cell Biology 2023Quote: ... 20 μl of eluted proteins were briefly run on SDS-PAGE (4 min at 160 V, 10% SDS gel [NP0301, Invitrogen]), stained with SimplyBlue SafeStain (LC6065 ...
-
bioRxiv - Microbiology 2023Quote: Four milliliters of each resuspension were pelleted via centrifugation (14,000 g, 10 min, 4 °C) (Heraeus Fresco 21, Thermo Scientific), the cells washed 4 times with ice-cold 0.9% (w/v ...
-
bioRxiv - Biochemistry 2023Quote: ... Samples were boiled at 95°C and 10 µL were then run on a 4–12% Bis-Tris NuPAGE gel (Invitrogen). Bands were visualized by staining with InstantBlue Coomassie Stain (Abcam ...
-
Identification of resistance mechanisms to small-molecule inhibition of TEAD-regulated transcriptionbioRxiv - Cancer Biology 2023Quote: ... at 4⁰C and centrifuged (18,000 x g and 4⁰C) for 10 minutes prior to protein quantification by BCA assay (ThermoFisher Scientific). Proteins in lysates (1µg/ml ...
-
bioRxiv - Immunology 2024Quote: ... centrifuged at 500g for 10 minutes at 4°C and resuspended in 0.25% Trypsin-EDTA (Thermo Fisher Scientific, #25200-072) at 37°C for 10 minutes ...
-
bioRxiv - Neuroscience 2022Quote: 10 μL of normalized lysate with loading buffer was loaded into NuPAGE 4 to 12% Bis-Tris protein gels (Invitrogen) and subjected to 100 V electrophoresis for 65 min ...
-
bioRxiv - Immunology 2022Quote: ... was dialyzed for 48 h at 4°C into PBS using 10 kDa MWCO Slide-a-lyzer cassettes (Thermo Fisher) before i.n ...
-
bioRxiv - Cell Biology 2024Quote: ... and pCRSIPR-Lenti-V2 by electroporation using the NEON system (Thermo Fisher, buffer N, 4 pulses, 10 ms, 1230 V). After electroporation ...
-
bioRxiv - Biochemistry 2023Quote: ... INPUT and UNBOUND (both 10 % of initial protein extract) and BOUND (IP) fraction were resolved by electrophoresis on acrylamide gels (4-12% Invitrogen) and the transferred onto nitrocellulose membrane that were saturated for 1 hour ...
-
bioRxiv - Bioengineering 2024Quote: Organoids cultured for 63 days in static and dynamic conditions were stained with 10 µM of Fluo-4 AM cell permeant dye (F14201, ThermoFisher) for calcium imaging ...
-
bioRxiv - Bioengineering 2024Quote: ... Cells were centrifuged at 500 ×g for 5 minutes at 4°C and washed and resuspended with ice-cold FACS Buffer (2 or 10% FBS in 1× HBSS (Gibco) or 1X PBS) ...
-
bioRxiv - Cell Biology 2024Quote: ... and fixed for 10 min by 4% paraformaldehyde (PFA) diluted in Dulbecco’s PBS containing Ca2+ and Mg2+ (DPBS; Gibco 14040083), then blocked and permeabilized for 1 h in blocking buffer (10% bovine calf serum and 0.1% Triton X-100 in DPBS) ...
-
bioRxiv - Molecular Biology 2024Quote: ... for 10 minutes at 70°C before loading on a Bolt Bis-Tris Plus 4 to 12% gel (ThermoFisher Scientific) for total protein staining or Western blotting ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cells were spread onto superfrost plus microscope slides (Epredia) at 2,000 rpm for 10 min by Cytospin 4 (Thermo Scientific) or Cellspin 1 (Tharmac) ...
-
bioRxiv - Immunology 2024Quote: ... Cells were counted and 4 million cells were plated in 10 cm non-TC treated plates in 8 mL RPMI with HEPES (Gibco), Sodium Pyruvate (Gibco) ...
-
bioRxiv - Microbiology 2024Quote: ... we pelleted samples by centrifugation at 5,000 x g for 10 minutes at 4°C and then resuspended the pellets in RNAlater (Invitrogen AM7021); samples were incubated in RNAlater at 4°C for 24 h ...
-
bioRxiv - Immunology 2024Quote: ... Cells were fixed for 10 min in 4% PFA and were acquired on an Attune NxT flow cytometer (Life Technologies). Results were analyzed with the FlowJo V10 software ...
-
bioRxiv - Molecular Biology 2024Quote: ... Lysate was centrifuged at 10000 g at 4°C for 10 minutes and supernatant was transferred to Dynabeads M-270 (Invitrogen) coupled to either CTD Ser2P (C15200005 ...
-
bioRxiv - Microbiology 2023Quote: ... Bacteria were centrifuged for 10 min at 10,000 x g (4°C) and the cells resuspended in 0.5 ml of TRIzol (Thermo Fisher Scientific). Direct-zol ...
-
bioRxiv - Immunology 2023Quote: ... 10/20µg of protein sample was loaded in the wells of precasted 4-12% polyacrylamide NuPAGE Novex gel (Invitrogen, NP0322BOX) submerged in 1X MOPs running buffer ...
-
bioRxiv - Biochemistry 2023Quote: ... and 10% 2-Mercaptoethanol or 50 mM dithiothreitol (DTT) and run on NuPAGE 4-12% bis-tris gels (Thermo Fisher). Proteins were transferred to nitrocellulose membranes ...
-
bioRxiv - Systems Biology 2023Quote: ... and 10-15 µg of protein were loaded on 4-12% Bis-Tris Midi NuPage Protein Gels (Thermo Fisher Scientific) and separated at 120V for 2 h in MES running buffer ...
-
bioRxiv - Physiology 2024Quote: ... Slides were then washed 4 x 10 min in PBS and incubated with Goat anti-Rabbit 750 (A21039, Thermo Fisher) and DAPI in 3% BSA in PBS ...
-
bioRxiv - Physiology 2024Quote: ... sections were incubated overnight at 4°C in primary antibody solution containing 10% goat serum and antibodies against laminin β1 (1:100; MA5-14657, Invitrogen) or laminin β1γ1 (1:200 ...