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Citations for Thermo Fisher :
1551 - 1600 of 10000+ citations for tert Butyl 10 oxo 4 9 diazaspiro 4.5 decane 4 carboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Third instar larvae were dissected with cold HL3 and immediately fixed with PFA (4%) and incubated overnight at 4 C with primary antibodies (rabbit anti-Dlg, 1:1000; anti-Brp 1:100, Life Technologies). Alexa-conjugated secondary antibodies were used for secondary staining (Jackson Laboratories 1:500) ...
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bioRxiv - Cell Biology 2021Quote: ... and the pellets were dissolved in 0.1% SDS/ 0.67 M Tris-HCl pH 8 +/- 15 mM 4-acetamido-4’-maleimidylstilbene-2,2’-disulfonic acid (Thermo Fisher Scientific, USA). The samples were incubated at 37 °C for 2 h ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were centrifuged at 14,000g for 5 minutes at 4°C and electrophoresed on NuPAGE™ 4-12% Bis-Tris Polyacrylamide gels (Thermo Fisher) with NuPAGE™ MOPS running buffer (Thermo Fisher ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were ultracentrifuged for 4 hours at 50.000 rpm at 4°C in a Sorvall MX 120/150 Plus MicroUltracentrifuge (Thermo Fisher Scientific) in S55A2 rotor ...
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bioRxiv - Immunology 2020Quote: ... mice were anesthetized using isofluorane and perfused transcardially with 4 mL PBS followed by 4 mL RNAlater™ (Invitrogen; CAT # AM7020) using a peristaltic pump at a flow rate of 0.8mL/min ...
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bioRxiv - Biophysics 2021Quote: ... The grids were blotted at blotting force 20 for 4 s at 4°C and 100% humidity and plunged into liquid ethane using a Vitrobot (Thermo Scientific).
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bioRxiv - Synthetic Biology 2020Quote: ... Cellular response in each well was measured via fluo-4 intracellular calcium dye using the fluo-4 NW calcium assay kit (Molecular Probes) according to manufacturer’s directions ...
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bioRxiv - Biochemistry 2022Quote: Protein samples in 1X LDS sample buffer containing 4% β-mercaptoethanol (BME) were separated by size on 4-12% Novex NuPage Bis-Tris SPS-PAGE (Invitrogen) gels (2 h ...
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bioRxiv - Genetics 2022Quote: ... Larval brains were fixed in 25 ml of acetic acid: 4% formaldehyde in PBS (45%:55%) for 4 min on a clean Superfrost plus slide (Fisher Scientific) and the sample was manually squashed via thumb/stamp over coverslip ...
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bioRxiv - Plant Biology 2022Quote: ... from cauline leaves of flowering plants by measuring the quantity of 4-methylumbelliferone product generated from the substrate 4-methylumbelliferyl-b-D-glucuronide (Duchefa) on a fluorometer (Fluoroscan II; Thermo Scientific).
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bioRxiv - Evolutionary Biology 2022Quote: ... pseudonana expressing VHAB-eGFP were incubated with the acidotropic pH stain 2-(4-pyridyl)-5-((4-(2-dimethylaminoethyl-aminocarbamoyl)methoxy)phenyl)oxazole (PDMPO) (LysoSensor YellowBlue DND-160; Life Technologies) at a final concentration of 0.125 μM without washing in F/2 ...
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bioRxiv - Neuroscience 2022Quote: ... 20 μg of protein per lane was loaded and run on a 4–12% Bis-Tris PAGE gel (Bolt 4–12% Bis-Tris Protein Gel, Invitrogen, NW04122BOX) and transferred onto a PVDF membrane (Immobilon-FL ...
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bioRxiv - Neuroscience 2022Quote: ... Three successive 5-hour paraffin wax embedding stages were performed followed by cooling and sectioning of tissue on a Leica microtome into 4 × 4 μm thick serial sections that were collected on Superfrost (ThermoFisher Scientific) microscope slides ...
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bioRxiv - Molecular Biology 2022Quote: ... the mix was cooled down to 4°C and supplemented with 1 μl of a solution containing 4 U Terminal Transferase (Thermo Scientific), 0.1 μl 10X NEB 4 buffer (NEB) ...
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bioRxiv - Physiology 2024Quote: ... only 4 wells of cells were incubated in DMEM with 4 μM fura-2/AM (fura-2/AM, Molecular Probes, USA) at 37° C in the dark for 45 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Separation of anionic metabolites were performed using a Dionex IonPac AS11-HC-4 µm column (250 × 0.4 mm, 4 µm; Thermo Fisher Scientific). The injection volume was 0.4 µL ...
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bioRxiv - Cell Biology 2024Quote: ... Click-iT reaction cocktail (100 Mm Tris-buffered saline, 4 mM CuSO4, 100 mM ascorbic acid and 4 µM Alexa FluorTM488 azide (Invitrogen #A10266) was used for detecting EU ...
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bioRxiv - Neuroscience 2024Quote: ... larvae and dissected adult brains were fixed overnight in paraformaldehyde (PFA; 4% in 1x phosphate-buffered saline) at 4°C then dehydrated overnight in 100% methanol (A4124, Fisher Scientific) at -20°C ...
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bioRxiv - Cell Biology 2024Quote: ... hansenii cells were concentrated 10x in 200 μL YPD containing 40 μM FM4-64 dye (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) dye (Invitrogen™) to label endosomes for 8 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... (4) 30 min incubation in ammonium chloride (NH4Cl) and (5) 4 min incubation in Tissue Autofluorescence Quenching Kit (ReadyProbes, ThermoFisher Scientific).Secondary antibodies were used as follows ...
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bioRxiv - Immunology 2023Quote: ... Invitrogen E-Gel EX Agarose Gels 4%, and DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride, D1306) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... -4 blot force for 4 seconds and plunged frozen in liquid ethane using the FEI Vitrobot Mark IV (Thermo Fisher Scientific). Data were collected on a Titan Krios operating at 300 keV and equipped with a Gatan K3 direct detector and a 20 eV slit width energy filter ...
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bioRxiv - Plant Biology 2023Quote: ... Denatured samples were spun down at 5,000 xg for 3 minutes and supernatant was run on 4%-20% Bio-Rad 4%-20% Mini-PROTEAN TGX gels alongside a PageRuler Plus prestained protein ladder (Thermo Scientific). The proteins were then transferred to polyvinylidene difluoride membranes using Trans-Blot Turbo Transfer Buffer using a Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... MBL) for 4 days at 4°C and with Alexa-Fluor (AF) 488 goat anti-rabbit IgG (R37116, Thermo Fisher Scientific) overnight at RT followed by DAPI staining (1:10,000 ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged at 22,000 × g at 4°C for 20 minutes and poured over 4 mL of HisPur Cobolt resin (89964, Thermo Fisher Scientific). The slurry was rotated at 4°C for 1 hour and spun down at 700 × g for 2 minutes ...
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bioRxiv - Genetics 2023Quote: ... Extracts were incubated with rotation with rabbit polyclonal anti-Piwi antibodies (4 µg per sample) at 4°C for 4h followed by overnight incubation with DynabeadsTM Protein A (50 µl, Invitrogen, 10002D) at 4°C with rotation ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: A fraction of the liver from each mouse was preserved in 4% formalin (Cat# SF100-4, Thermo Fisher Scientific, Waltham, MA) followed by 70% ethanol for histology ...
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bioRxiv - Immunology 2023Quote: ... blotted for 4 s in 100% humidity at 4 °C and plunged into liquid ethane using an FEI Vitrobot Mark IV (Thermo Fisher). All grids were screened using a ThermoFisher Glacios microscope (OSU Center for Electron Microscopy and Analysis).
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bioRxiv - Biochemistry 2023Quote: ... NaHCO3 and 150 μL acetone containing 4 mg/mL 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide (L-FDAA, Thermo Scientific) was added ...
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bioRxiv - Cell Biology 2023Quote: ... Extracts were clarified by centrifugation twice for 15 minutes at 16,000 rcf at 4°C and 100 μl embryo extract was rotated at 4°C overnight with 30 μl magnetic ProteinA dynabeads (Life Technologies) coupled with anti-MBP antibodies (gift from Jordan Raff ...
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bioRxiv - Immunology 2023Quote: ... Drosophila S2 cells were mixed with Ramos cells or B1-8hi B cells at a ratio of 1:4 (4×105 B cells and 1×105 S2 cells) followed by total RNA extraction with TRIzol reagent (Thermo Fisher), DNaseI digestion ...
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bioRxiv - Biophysics 2023Quote: ... Samples were mildly fixed with 4% PFA for 4 min before immunostaining using anti SARS-CoV-2 Spike Protein S1 (Cat# PA5-114447, ThermoFisher Scientific) and anti-human IgG (Fc)-Alexa 488 (Cat# 109-545-170 ...
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: Colon crypts from 4 to 7 months old mice were isolated as described above and then resuspended in 4 ml of TryplExpress (ThermoFisher Scientific) for 15 min at 37°C under agitation at 75 rpm ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were washed with ice-cold PBS, harvested, and incubated (30 min, 4 °C) with rabbit anti-AF488 IgG (4 µg/mL, Thermo Fisher). AF488 fluorescence was quantified by flow cytometry (BD FACSCanto II flow cytometer ...
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bioRxiv - Cancer Biology 2023Quote: ... with a Dionex IonPac AS11-HC-4 μm column (2 mm i.d. × 250 mm, 4 μm particle size, Thermo Fisher Scientific) coupled with a Q Exactive ...
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bioRxiv - Plant Biology 2023Quote: ... Denatured samples were spun down at 5,000 g for 3 min and supernatant was run on 4–20% Bio-Rad 4–20% Mini-PROTEAN TGX gels alongside a PageRuler Plus prestained protein ladder (Thermo Scientific). The proteins were then transferred to polyvinylidene difluoride membranes using Trans-Blot Turbo Transfer Buffer using a Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Systems Biology 2023Quote: ... with a Dionex IonPac AS11-HC-4 μm column (2 μm i.d. × 250 mm, 4 μm particle size, Thermo Fisher Scientific) coupled with a Q Exactive high-performance benchtop quadrupole Orbitrap mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Antibody or IgG (as control) was added and incubated for 4 hrs at 4°C with Protein G Dynabeads (Life Technologies) for antigen coupling ...
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bioRxiv - Plant Biology 2024Quote: ... Denatured samples were spun down at 5,000 xg for 3 minutes and supernatant was run on 4%-20% Bio-Rad 4%-20% Mini-PROTEAN TGX gels alongside a PageRuler Plus prestained protein ladder (Thermo Scientific). The proteins were then transferred to polyvinylidene difluoride membranes using Trans-Blot Turbo Transfer Buffer using a Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: Perfused brains were post-fixed overnight at 4°C in 4% PFA then cryoprotected in 30% (w/v) sucrose (Fisher Scientific) solution in PBS for at least 48 hr prior to processing ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The tissue specimens were fixed with 4% PBS-buffered (pH 7.4) paraformaldehyde for 12 h at 4°C before immersion in 30% sucrose and freezing in OCT (LAMB/OCT, Thermo Scientific) in cryomolds ...
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bioRxiv - Neuroscience 2024Quote: ... sham and uPA-CBP were anesthetized (4% isoflurane, 2 liters of oxygen) and transcardial perfusion fixed with 4% paraformaldehyde (PFA) (Cat #50-980-494, Fisher Scientific) in PBS 1x until stiff ...
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bioRxiv - Neuroscience 2024Quote: ... Brain slices were then fixed for 30 minutes in 4% PFA in PBS at 4 and labeled with streptavidin conjugated with Alexa Fluor 568 (ThermoFisher S11226) for three hours at room temperature ...
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bioRxiv - Neuroscience 2024Quote: Primary mouse and human iPSC-derived astrocytes were incubated with Fluo-4 calcium assay reagent (Fluo-4 Direct™ Calcium-Assay-Kit, ThermoFisher) for 30 min at 37°C before recording baseline fluorescence levels (RFUMin ...
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bioRxiv - Bioengineering 2024Quote: ... The next day the membrane was washed three times with 5% milk and incubated for 4 hours at 4°C in an anti-rabbit secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... with a Dionex IonPac AG11-HC-4 μm guard column (2 mm i.d × 50 mm, 4 μm particle size, Thermo Fisher Scientific) and a Dionex IonPac AS11-HC-4 μm column (2 mm i.d × 250 mm ...
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bioRxiv - Developmental Biology 2024Quote: ... and a Dionex IonPac AS11-HC-4 μm column (2 mm i.d × 250 mm, 4 μm particle size, Thermo Fisher Scientific) coupled to a Q Exactive mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: Cell monolayers or tissues were fixed for 20 minutes at 4 °C with 4% (w/v) paraformaldehyde (Affymetrix, Santa Clara, CA), then permeabilized with PBS 0.2% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... incubated for 24h at 4°C in secondary antibodies (4 µg/ml goat anti-mouse Alexa Fluor 568 (Thermo Fisher Scientific) and 4 µg/ml goat anti-rabbit Alexa Fluor 488 (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... assessment of both live and dead cells on the N27 cell line was performed with a combination of 10 μM CellTracker green and 4 μM Propidium Iodide (PI, Invitrogen, ThermoFisher) solutions ...