Labshake search
Citations for Thermo Fisher :
1701 - 1750 of 10000+ citations for Glutathione Fluorescent Detection Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... Fluorescent cell nuclei staining was performed with Hoechst 33342 (Invitrogen) according to the manufacturer instructions ...
-
bioRxiv - Neuroscience 2024Quote: ... Appropriate fluorescent Alexa Fluor secondary antibodies were purchased from Invitrogen.
-
bioRxiv - Microbiology 2024Quote: ... additional fluorescent probes were utilized: Sytox Blue (S11348, Invitrogen, USA), Sypro Ruby (F10318 ...
-
bioRxiv - Neuroscience 2024Quote: ... All fluorescent-conjugated secondary antibodies were purchased from Thermo Fisher Scientific (Supplementary Table 1).
-
bioRxiv - Developmental Biology 2024Quote: ... Feeder-depleted serum cultured ESCs were plated on gelatin-coated plates at 10,000 cells/cm2 (90,000 cells per well in a 6-well plate) in advanced N2B27 defined media composed of a 1:1 mix of 1:1 DMEM/F12 (Invitrogen, 12634-010) and Neurobasal medium (Invitrogen ...
-
bioRxiv - Immunology 2020Quote: ... Plates were developed using 1-Step Ultra TMB-ELISA (34028, Thermo Fisher) for one to two minutes after which the reaction was stopped with 1M H2SO4 ...
-
bioRxiv - Neuroscience 2021Quote: ... on six-well plates coated with 1:100 Geltrex (Life technologies; A1413302). Media was changed every 48 hours and passaged when 70-80% confluent with HBSS and Versene (Gibco ...
-
bioRxiv - Immunology 2020Quote: ... The plate was blocked with PBS+1%BSA (Fisher Scientific, PA, USA)+0.1%Tween20™ (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2022Quote: ... HepG2 cells (1×105) were seeded in 24-well plates (ThermoFisher Scientific). Two days after the second silencing (Mock or siTANGO2) ...
-
bioRxiv - Neuroscience 2024Quote: ... 12-well plates were coated overnight with 1% GelTrex (Thermo Fisher Scientific) diluted in H2O ...
-
bioRxiv - Immunology 2024Quote: ... plates were developed using TMB 1-Component Peroxidase Substrate (Thermo Fisher Scientific), followed by reaction termination using the TMB stop solution (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2023Quote: ... Plates were developed using 150μl 1-Step ABTS substrate (ThermoFisher, Prod#37615). Following a brief incubation at room temperature ...
-
bioRxiv - Microbiology 2024Quote: ... Plates were developed using 150μl 1-Step ABTS substrate (ThermoFisher, Prod#37615). Following a brief incubation at room temperature ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... plates were washed 1 × with Neurobasal A with B-27 (Life Technologies) and 0.5 mM Glutamax and replaced with glial conditioned Neurobasal A medium containing B27 ...
-
Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... Real-time PCR detection of mRNA expression was performed using a 7500 Sequence Detection System (Applied Biosystems, Foster City, CA). Specific primers (10 μM ...
-
bioRxiv - Microbiology 2020Quote: ... The position of the Nucleic acids was visualized by chemiluminescent detection using the Chemiluminescent Nucleic Acid Detection Module (Thermo Scientific) following manufacturer’s instructions and exposed to X-ray film (Amersham hyperfilm ECL ...
-
bioRxiv - Molecular Biology 2024Quote: ... Immunoreactive bands were visualized by detection of chemiluminescent signals after addition of the Pierce ECL detection system (Thermo Fisher Scientific). All ELISA and Western blot analyses were confirmed in at least 3 independent experiments with individuals originating from different colonies.
-
bioRxiv - Physiology 2020Quote: ... Fluorescent signals were obtained by peroxidase-mediated Alexa Fluor 488 tyramide labelling (Alexa fluor 488 Tyramide SuperBoost kit, Thermofisher Scientific, Cat no. B40922). Slides were counterstained with DAPI and mounted in ProLong Diamond antifade mounting medium (Thermofisher Scientific ...
-
bioRxiv - Developmental Biology 2021Quote: ... The remaining cells were respectively labelled with aqua-fluorescent reactive dye (dying cells) and calcein violet AM (living cells) using the LIVE/DEAD Violet Viability/Vitality Kit (Molecular Probes, ref. L34958) under manufacturer’s guidelines ...
-
bioRxiv - Microbiology 2022Quote: ... and the ratio of viable and dead cells was evaluated using the fluorescent LIVE/DEAD® BacLight™ viability kit (ThermoFisher scientific, USA), as described in our previous works (Nagy et al ...
-
bioRxiv - Plant Biology 2024Quote: ... while its concentration was estimated using a fluorescent DNA intercalating agent (e.g., Quant-iT PicoGreen dsDNA Assay Kit, Thermo Fisher Cat. No. P7589) and a microplate reader (Thermo Fisher Scientific)) ...
-
bioRxiv - Immunology 2024Quote: ... 1-Step Kit (ThermoFisher Scientific) and StepOne Plus Real-Time PCR system (Applied Biosystems) ...
-
bioRxiv - Microbiology 2019Quote: ... Stress response was assessed by measuring glutathione levels using 5-chloromethylfluorescein diacetate (Cell tracker™ green CMFDA, Life Technologies) as already shown in mammalian cells [26,87] and yeasts [23,27] ...
-
bioRxiv - Cell Biology 2021Quote: ... and GST-tagged baits contained in supernatants were normalized using Pierce Glutathione Magnetic Agarose Beads (Thermo Fisher Scientific, #78602) according to manufacturer’s protocol ...
-
bioRxiv - Immunology 2022Quote: ... Phages presenting NLRP1PYD-specific VHHs were enriched using GST-NLRP1PYD immobilized to magnetic Pierce glutathione beads (Thermo Fisher Scientific). The retained phages were used to infect E ...
-
bioRxiv - Neuroscience 2021Quote: ... Lysates were centrifuged at 20,627g for 20 min at 4°C and incubated with glutathione agarose beads (Thermo Scientific) overnight at 4°C ...
-
bioRxiv - Plant Biology 2022Quote: ... the membrane was then incubated for 1 h in a blocking solution composed of 0.5 % semi-skimmed milk powder in TBST (Tris-buffered saline 20 mM, 0.1 % Tween 20) and then incubated with the anti-glutathione (Invitrogen) primary antibody solution at 4 °C overnight ...
-
bioRxiv - Microbiology 2023Quote: ... Anti-GST pull-downs were completed with these protein suspensions subsequently incubated with Pierce™ Glutathione Agarose (ThermoFisher 16100) for an hour at 4°C in binding buffer (PBS pH 7.0 with 1 mM Tris (2-carboxyethyl ...
-
bioRxiv - Microbiology 2023Quote: ... GST-RILP or GST proteins were purified by using a pre-equilibrated slurry of glutathione beads (Thermo Scientific, #16100) in TBS containing 1mM MgCl2 and washed three times in the same buffer containing 0.05% Triton X-100 ...
-
bioRxiv - Biophysics 2023Quote: ... The supernatant was clarified by filtering through a 0.44 ×G μm PVDF syringe filter and then incubated with 2 mL of Pierce glutathione agarose resin (16100, ThermoFisher) for 2 hr at 4 °C in a 5 mL polypropylene column ...
-
bioRxiv - Cell Biology 2024Quote: Overexpression of glutathione S-transferase (GST) fusion proteins was induced in DH5α Escherichia coli cells (Life Technologies, MD, USA) using 0.4mM isopropyl β-D-thiogalactopyranoside and cells subsequently broken by sonication ...
-
bioRxiv - Cancer Biology 2021Quote: ... on the ABI 7900 detection system (Applied Biosystems). Relative expression values were determined using the standard curve method and were normalized to housekeeping gene Rplp0 ...
-
bioRxiv - Molecular Biology 2021Quote: ... proteins were visualized by chemiluminescent detection (Thermo Scientific).
-
bioRxiv - Developmental Biology 2022Quote: ... Visualization was done by ECL detection (Thermo Scientific).
-
bioRxiv - Developmental Biology 2019Quote: ... Visualization was done by ECL detection (Thermo Scientific). Whole mount immunofluorescence on Hydra polyps was done as described previously [48] ...
-
bioRxiv - Genomics 2021Quote: ... and an 7900HT sequence detection system (Applied Biosystems). Expression levels were normalized with the housekeeping gene Gapdh ...
-
bioRxiv - Bioengineering 2020Quote: ... was performed using TaqMan probe detection (Applied Biosystems). Real-time PCR was done for glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
-
bioRxiv - Neuroscience 2020Quote: ... and the QuantStudio 3 detection system (Applied biosystems). Primer sequences are listed in Table S1 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Antibodies and detection: OXPHOS (2μg/ml, Invitrogen, USA); ERα D8H8 (1μg/ml ...
-
bioRxiv - Cancer Biology 2019Quote: ... on the ABI 7900 detection system (Applied Biosystems). Relative expression values were determined using the standard curve method ...
-
bioRxiv - Cell Biology 2021Quote: ... Bands were revealed using ECL detection reagent (ThermoFisher), with images acquired using the ChemiDoc MP system (Bio-Rad) ...
-
bioRxiv - Cell Biology 2022Quote: ... Supersignal West Femto detection reagent (Thermo Fisher Scientific) was used to generate a signal ...
-
bioRxiv - Immunology 2020Quote: Detection of human IL-1β and TNFα (Invitrogen) in hMDM culture supernatants were performed by sandwich ELISA ...
-
bioRxiv - Neuroscience 2019Quote: ... and chemiluminescence detection reagent ECL (Thermo Fisher Scientific).
-
bioRxiv - Developmental Biology 2020Quote: ... on the 7900HT Sequence Detection System (Applied Biosystems). Average Ct values were calculated from quadruplicates of each sample ...
-
bioRxiv - Microbiology 2020Quote: ... Detection was performed using Chemiluminescent Substrate (Thermo Fisher).
-
bioRxiv - Immunology 2022Quote: ... CD20 (clone L26, ThermoFisher, detection with Opal 480) and DAPI (nuclear marker) ...
-
bioRxiv - Cell Biology 2020Quote: ... on a ViiA7 qPCR detection system (Life Technologies). All reactions for RT-qPCR were performed using the following conditions ...
-
bioRxiv - Microbiology 2020Quote: ... ABI PRISM 7500 Sequence Detection System (Applied Biosystems).Amplifications were carried out in 25 μL reaction mixtures containing 2× reaction mix buffer ...
-
bioRxiv - Developmental Biology 2020Quote: ... with the 7900HT Sequence Detection System (Applied Biosystems). Primers used for all qRT-PCR analyses are listed in Supplemental Table S1.