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Citations for Thermo Fisher :
1851 - 1900 of 10000+ citations for Glutathione Fluorescent Detection Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... the tissue was stained with NeuroTrace™ green fluorescent Nissl stain (1:200 in PBS, Life Technologies, catalog No. N-21480) for 20 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were next incubated in fluorescent secondary antibodies: AlexaFluor Plus 488 goat anti-mouse IgG (H + L) (1:500; A32723; ThermoFisher Scientific) and Texas Red Streptavidin conjugate (1:1000 ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... The recording sites were verified by the staining with DAPI and red fluorescent Nissl stain solution (1:200 dilution; Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... for 1h at RT and stained o/n with antibody fluorescent conjugates (1:500, anti-mouse-AF488, anti-rabbit-AF555 or 647, anti-rat- AF546, all from Thermo-Fisher Scientific), 1mg/ml DAPI solution 1:500 and PNA-lectin AF594 1:1000 (1mg/ml ...
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bioRxiv - Immunology 2021Quote: ... in a 1:1 bead to T cell ratio and cultured in 2 ml (24-well plate) or 1 ml (48-well plate) RPMI 1640 (Thermo Fisher, 21875) supplemented with 10% Fetal Bovine Serum (Thermo Fisher ...
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bioRxiv - Genetics 2022Quote: ... 24-well plates containing 500 µL of antibiotic-free (DMEM)/F12 (1:1) GlutaMAX™ (ThermoFisher Scientific) were incubated at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... qPCR was conducted in 384-well plates using the SensiFAST SYBR Lo-ROX Kit (Meridian Bioscience) on the QuantStudio 5 (384-well plate) qPCR cycler (Applied Biosystems, Foster City, CA, USA). The qPCR program encompassed 40 cycles ...
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bioRxiv - Cancer Biology 2021Quote: ... Detection of primary antibodies was performed with Alexa Fluor 488 Goat anti-Mouse IgG (1:2000 Thermo Fisher Scientific) and Alexa Fluor 594 Goat anti-Rabbit IgG (1:2000 Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... at a dilution of 1:500 and secondary detection was performed using goat anti-rat Alexa 488 antibodies (Invitrogen). Fiber area was measured by using ImageJ software ...
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bioRxiv - Cell Biology 2020Quote: ... goat anti-mouse IgG (1:5000) and detection of protein was conducted using ECL Western blotting reagents (Thermo Fisher). All Western blots are representative images from at least three biological replicates.
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bioRxiv - Cancer Biology 2022Quote: ... Detection was performed using the following secondary antibodies: Alexa Fluor 488 donkey anti rabbit (1:500 dilution; A21206, Invitrogen), Alexa Fluor 594 donkey anti mouse (1:500 dilution ...
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bioRxiv - Microbiology 2020Quote: ... was used as secondary antibody (1:10,000 dilution) and the detection was achieved with the ECL reagent (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2019Quote: ... Detection was performed with a 1h incubation at RT with secondary antibodies AlexaFluor488 or AlexaFluor594 (1:500; Molecular Probes), and DAPI (1:500 ...
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bioRxiv - Cell Biology 2019Quote: ... secondary antibodies coupled to horseradish peroxidase were added for 1 h at RT before ECL detection (Thermo Fisher Scientific). Immunoblots were quantified using ImageJ.
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bioRxiv - Genetics 2020Quote: ... and the following primary antibodies and dilutions were used for detection: mouse anti-V5 (Invitrogen R960-25, 1:5,000) and rabbit anti-Hxk2 (Rockland #100-4159 ...
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bioRxiv - Cancer Biology 2022Quote: ... alongside aforementioned activating proteins and CellEvent™ Caspase-3/7 Green Detection Reagent (1:5000, ThermoFisher Scientific, Cat#C10423). Cells were imaged using the IncuCyte ZOOM System (Essen Bioscience ...
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bioRxiv - Neuroscience 2022Quote: ... overnight at 4 °C followed by detection with Alexa 488 conjugated anti-rat secondary antibody (1:1000, Life Technologies). Arteries were identified by immunostaining with a mouse monoclonal anti actin ...
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bioRxiv - Biophysics 2023Quote: ... 40 μl of each sample was used for Western blotting detection with 1:2,500 anti-GFP antibody (Invitrogen A11122) and 1:10,000 anti-rabbit secondary antibody (Licor 926-68073) ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by secondary antibody for 1 hour and processed for protein detection using SupersignalTM West Pico PLUS (Thermo Scientific) or Immobilon Western chemiluminescent substrate (Millipore Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... Signal detection was performed using Alexa 546-conjugated donkey anti-mouse secondary antibody (1:1,000, 90 min, Molecular Probes). A Nikon Eclipse E600 fluorescent microscope equipped with appropriate filters (EX 465-695 ...
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bioRxiv - Molecular Biology 2024Quote: ... was performed before incubation overnight with primary antibody (Wako Chemicals RRID:AB_839504; 1:250) and detection with IgG conjugated to Alexa 488 (Invitrogen A11008). After washing in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... Slices were washed three times with PBS and incubated for 1 hr at room temperature with a fluorescent secondary antibody (Alexa Fluor 546 goat anti-rabbit, Thermo Fisher Scientific catalog #A-11010, 1:500). Slices were washed three times with PBS and mounted onto microscope slides with Prolong Gold anti-fade medium (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... followed by its reaction with detection reagents (ECL Western Blotting Detection Reagents, GE Healthcare or SuperSignalTM West Dura, Thermo Fisher Scientific). The primary antibodies used and their dilutions are listed in Supplementary file 3 ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were resuspended in water for detection on the Integron High Performance Ion Chromatography (HPIC) with suppressed conductivity detection (Thermo Fisher) as described previously69,70.
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bioRxiv - Molecular Biology 2024Quote: ... membranes were briefly rinsed with PBS and then subjected to detection using SuperSignal WestPico Plus ECL-based detection (ThermoFisher, cat# 34580). Finally ...
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bioRxiv - Microbiology 2019Quote: ... PCR amplicons were cleaned and normalized using SequalPrep Plate Normalization Kit (Invitrogen, Carlsbad, CA, USA) and combined into four pools ...
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bioRxiv - Microbiology 2022Quote: ... The amplicons were normalized using a SequalPrep™ Normalization Plate Kit (Thermo Fisher Scientific Inc.) and multiplexed.
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bioRxiv - Microbiology 2019Quote: ... Plates were scraped and transformant plasmids isolated using the GeneJET plasmid purification kit (Thermo scientific).
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bioRxiv - Microbiology 2022Quote: ... Sample normalization of the indexed amplicons was performed with the SequalPrep normalization plate kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... and successfully amplified libraries were normalized using SequalPrep™ Normalization Plate Kit (Thermo Fisher, USA). Normalized amplicons were then pooled and concentrated 20:1 using the 50K Dalton Millipore filters (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were cleaned and normalized using the SequalPrep Normalization Plate Kit (Invitrogen, Massachusetts, USA) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... ELISA plates (96-well plates, Immunol4HBX, Thermo Fisher) were coated with a 50/50 mixture of His-Spy-RBD and RBD-Spy-His (2 μg/mL ...
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bioRxiv - Microbiology 2019Quote: ... SAB agar plates (5-cm plates, Fisher Scientific) inoculated with 200 μL of a M ...
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bioRxiv - Bioengineering 2020Quote: 96-well plates (Nunc MediSorp plates; Thermo Scientific) were coated overnight at 4 °C with 50 ng/well of purified antigen (recombinant RSVF or designed immunogens ...
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bioRxiv - Bioengineering 2020Quote: 96-well plates (Nunc MediSorp plates; Thermo Scientific) were coated overnight at 4 °C with 50 ng/well of purified antigen (recombinant RSVF or designed immunogens ...
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bioRxiv - Immunology 2020Quote: ... Maxisorb 96-well plates (Nunc-immuno plates, Dutscher) were coated overnight at room temperature (RT ...
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bioRxiv - Immunology 2022Quote: ... Nunc Immuno Plate MaxiSorp plates (Thermo Fisher Scientific) were coated either with 50 μg/ml NP4-BSA or 50 μg/ml NP26-BSA and incubated overnight at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... Custom PCR plates (TaqMan 96 well-plate, ThermoFisher) were used to assess mRNA expression on a QuantStudio 12K Flex Real-Time PCR System (ThermoFisher ...
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Micro topographical instruction of bacterial attachment, biofilm formation and in vivo host responsebioRxiv - Microbiology 2022Quote: ... The viability of attached cells was evaluated by fluorescent staining with the LIVE/DEAD® BacLight™ bacterial viability kit (Molecular Probes, Life Technologies).
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Micro topographical instruction of bacterial attachment, biofilm formation and in vivo host responsebioRxiv - Microbiology 2022Quote: ... The viability of attached cells was evaluated by fluorescent staining with the LIVE/DEAD® BacLight™ bacterial viability kit (Molecular Probes, Life Technologies).
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bioRxiv - Synthetic Biology 2020Quote: Ten percent of the final RNA amount was used for chemical labelling with Cy3 fluorescent dye using Arcturus Turbo Labeling Cy3 Kit (ThermoFisher, Applied Biosystems, Cat No. KIT0609). The chemical labelling procedure and subsequent removal of free Cy3 dye was performed following manufacturer’s protocol with 15 μg of RNA per reaction ...
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bioRxiv - Developmental Biology 2023Quote: ... EdU-labelled cells were fluorescently marked by click-it chemistry with the specific addition of a fluorescent azide on EdU molecules (Click-iT™ EdU Cell Proliferation Kit, 488 or 555 dye, ThermoFisher, #C10337 and #C10638), following (Demilly et al. ...
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bioRxiv - Cell Biology 2024Quote: ... all libraries were quantified using the highly sensitive fluorescent dye-based Qubit® ds DNA HS Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) and sequencing was performed using the illumina NovaSeq 6000 system (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... The membranes were developed after 1 hour of secondary antibody incubation at 1:5000 dilution by using ECL™ Prime Western Blotting Detection Reagent (Fisher Scientific, Catalog no. RPN2232).
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bioRxiv - Developmental Biology 2024Quote: mESCs were plated on gelatin-coated plates at a final density of 1 × 104 cells/cm2 in N2B27 medium (1:1 mix of DMEM/F12 (Gibco, 21331020) and Neurobasal medium (Gibco ...
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bioRxiv - Biochemistry 2021Quote: ... Sample was spun at 29,000 RCF at 4°C and supernatant was incubated with rocking with Pierce Glutathione Agarose Resin (Thermo Fisher) for 2-3 hours ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were centrifuged for 10 minutes at 10,000 rpm and the clarified lysates loaded onto a glutathione resin and purified by batch purification according to the manufacturer’s protocol (ThermoFisher, PI16101). Purified proteins were then dialyzed against Lysis Buffer to remove GSH and quantified using a Bradford assay per the manufacturer instructions (BioRad ...
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bioRxiv - Systems Biology 2022Quote: ... we used either ‘ThiolTracker Violet’ to stain intracellular glutathione (at 20 µM final concentration, Thermo Fisher scientific Cat. No. T10095), ‘mitoSOX red’ to stain intracellular superoxide (at 5 µM final concentration ...
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bioRxiv - Cancer Biology 2020Quote: ... washed twice with DPBS and incubated with 0.25 mg/ml of EZ-Link Sulfo-NHS-SS-Biotin cross-linking reagent (Thermo # 21331) in DPBS supplemented with 0.1 mM oxidized glutathione (Thermo Fisher) for 30 min at room temperature under constant rocking ...
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bioRxiv - Biochemistry 2020Quote: ... in a donor vector (pDONR-221) and transferred by recombinant cloning into the glutathione S-transferase (GST) fusion vector pDEST15 (Invitrogen). The plasmid was used to transform Escherichia coli ...