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Citations for Thermo Fisher :
1901 - 1950 of 10000+ citations for Glutathione Fluorescent Detection Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: The TNY1 cDNA coding sequences were cloned into the Gateway pDEST15-GST (glutathione S-transferase) plasmid using the procedures recommended by the manufacturer (Invitrogen) with oligos listed in Supplementary Table 1 ...
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bioRxiv - Plant Biology 2023Quote: ... GST-TUBE was added to the column retentate to a final concentration of 200 µg/ml before overnight glutathione agarose (ThermoFisher) capture at 4°C with gentle agitation ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was isolated by centrifugation for 30 min at 4°C at 18,500 × g and purified by glutathione affinity chromatography (Thermo Fisher) on a gravity column ...
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bioRxiv - Microbiology 2023Quote: ... Green fluorescence emission is indicative of the thiol-reactive chloromethyl group of the CM-H2DCFDA probe reacting with glutathione and other thiols (Invitrogen).
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bioRxiv - Plant Biology 2023Quote: ... Recombinant GST-tagged proteins purification were performed according to the manufacturer’s instructions of PierceTM Glutathione Superflow Agarose (Thermo Fisher Scientific), and recombinant MBP-tagged proteins purification were performed according to the manufacturer’s instructions of Amylose Resin (NEB).
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bioRxiv - Cell Biology 2024Quote: ... GST-RILP or GST proteins were purified by using a pre-equilibrated slurry of glutathione agarose (Thermo Fisher Scientific, 16100) in Buffer I [50 mM Tris pH8.0 ...
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bioRxiv - Microbiology 2023Quote: ... Plates (Nunc maxisorp ...
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bioRxiv - Neuroscience 2024Quote: ... Plates (Nunc Immuno clear modules ...
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bioRxiv - Molecular Biology 2020Quote: ... We then diluted these saturated cultures 1:20 in sterile water and then inoculated 3 µL into 96-well humidified plates (Nunc Edge Plates (Thermo Scientific, Waltham, MA)) with 150 µL of YPD or SD-CSM per well ...
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bioRxiv - Neuroscience 2020Quote: ... ECL was added to the plate and luminescence was measured with 1 s integration time using Varioskan Flash plate reader (Thermo Fisher Scientific, USA). Levels of TRKB:PTPσ interaction were evaluated by ELISA using a similar protocol ...
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bioRxiv - Microbiology 2019Quote: ... Then the O-GlcNAcylation level of the labeled EZH2-S73/S75-S725A-flag was analyzed by Click-iT™ Protein Analysis Detection Kits (Invitrogen, C33370).
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bioRxiv - Neuroscience 2019Quote: ... Sections were counterstained using DAPI provided in the V1 detection reagent kits and mounted in ProLong Gold mounting media (ThermoFisher Scientific P36934). Single-plane tiled images covering the DRN were scanned using a confocal microscope (Leica SP8X ...
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bioRxiv - Microbiology 2019Quote: ... All cells were maintained in a humidified incubator at 37°C and 5% CO2 and were found free of mycoplasma contamination on repeated testing with the MycoFluor Mycoplasma Detection Kit (Life Technologies, UK). MDCK II cells were treated as previously19 with the following modifications ...
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bioRxiv - Developmental Biology 2019Quote: ... LOX activity in 15 μl of concentrated conditioned media was determined using the Amplex Red H2O2 detection kit following the manufacture’s instructions (Life Technologies, Carlsbad, CA). This assay is based on the ability of endogenous LOX to oxidize 10 mM DAP (a LOX substrate ...
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bioRxiv - Immunology 2021Quote: ... cells were subjected to Click chemistry based EdU detection using the Click-iT Plus EdU Alexa Fluor 488 Flow Cytometry Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Annexin V apoptosis and dead cell detection assay was performed with the Alexa Fluor 488 Annexin V/Dead Cell Apoptosis Kit (Invitrogen, cat# V13241) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: Annexin V and 7AAD staining was performed using eBioscience™ Annexin V Apoptosis Detection Kit eFluor™ 450 (Invitrogen, 88-8006-72). Briefly ...
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bioRxiv - Developmental Biology 2021Quote: ... Detection of EdU was performed using the Click-iT EdU Cell Proliferation kit with Alexa Fluor 647 dye (Thermo Fisher Scientific, C10340) in accordance with the manufacturer’s instructions.
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bioRxiv - Genomics 2019Quote: The total ATP concentration of frozen-thawed spermatozoa was analyzed using the ATP Determination Kit A22066 (Molecular Probes TM Invitrogen detection technologies) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: The total ATP concentration of frozen-thawed spermatozoa was analyzed using the ATP Determination Kit A22066 (Molecular Probes TM Invitrogen detection technologies) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... All these steps are described in the protocol proposed by the manufacturer (Annexin V FITC Apoptosis Detection Kit, Invitrogen, Carlsbad, CA, USA). After 10 minutes ...
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bioRxiv - Cell Biology 2020Quote: Apoptotic cell death was measured by an Alexa Fluor® 488 annexin V and propidium iodide (PI) apoptosis detection kit (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: The compound 5-(and-6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA, Image-iT LIVE Green Reactive Oxygen Species Detection Kit, Molecular Probes; Invitrogen, I36007) was used to visualise the in vivo production of ROS ...
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Cwl0971, a novel peptidoglycan hydrolase, plays pleiotropic roles in Clostridioides difficile R20291bioRxiv - Microbiology 2021Quote: ... the supernatants from different strains were collected and filtered with 0.22 μm filters used for LDH concentration detection with the Pierce™ LDH Cytotoxicity Assay Kit (Thermo Fisher, USA) according to the instructions of the manufacturer ...
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bioRxiv - Cell Biology 2019Quote: ... Detection of RNA was performed in a two-step RT-qPCR using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, # 4374966) and the SYBR green master mix ...
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bioRxiv - Cancer Biology 2020Quote: ... Permeabilization and EdU detection was carried out using the Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s manual ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative RT-PCR was performed using QuantiTect Probe RT-PCR Kit (Quiagen®) in an ABI PRISM 7500 Sequence Detection System (Applied Biosystems). Amplifications were carried out in 25 µL reaction mixtures containing 2× reaction mix buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... after which then genomic DNA was isolated for detection of gene editing efficiency using the GeneArt genomic cleavage assay kit (Invitrogen, catalog #A24372). Hep3Bcas9 cells had high cleavage efficiency and thus was chosen for generating knockout cell lines using CRISPR ...
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bioRxiv - Genomics 2024Quote: ... We then compared the performance of the different kits in terms of detection and quantification rates using standard RT-PCR (Applied Biosystems 7500 Fast Instrument, Thermo Fisher) and portable RT-PCR (Magnetic Induction Cycler quantitative PCR (Mic qPCR) ...
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bioRxiv - Cancer Biology 2024Quote: ... qRT-PCR was performed using DyNAmo ColorFlash SYBR Green qPCR Kit in the ABI Quant Studio 5 Sequence Detection System (Life Technologies, USA). The expression of the genes of interest was analyzed by the ΔΔCt method using ATP5G rRNA and GAPDH as internal control genes ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then washed twice with PBS and fixed with 2% PFA before being stained for β-galactosidase activity with the CellEvent™ Senescence Green Detection Kit (Invitrogen) per manufacturer instructions.
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bioRxiv - Cancer Biology 2023Quote: Fixed brain sections were rehydrated and underwent antigen retrieval before being processed for TUNEL assay using the Click-iT™ Plus TUNEL Alexa Fluor™ 647 Assay Kit for In Situ Detection (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The number of SIV copies was normalized to diploid genome cell equivalents co-determined by Q-RT-PCR using the TaqMan™ RNase P Detection Reagents Kit (Applied Biosystems), as described previously (11 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were counterstained using DAPI provided in the detection reagent kits and mounted in ProLong Gold mounting media (P36930, Thermo Fisher Scientific). The images were obtained using an Olympus FV3000 confocal laser scanning microscope with a 40X magnification oil immersion objective ...
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bioRxiv - Immunology 2023Quote: ... Lysates were boiled for 5 minutes (except lysates used for detection of electron transport chain complexes) and protein content was determined using a BCA kit (23225, Thermo Fisher Scientific). Proteins were separated by SDS-PAGE and transferred to a 45 µm PVDF membrane ...
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bioRxiv - Cell Biology 2024Quote: ... All cells were assessed for mycoplasma contamination at monthly intervals using the LookOut Mycoplasma Detection kit (MP0035, Thermo Fisher Scientific, Loughborough, UK).
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bioRxiv - Molecular Biology 2024Quote: TUNEL assays were performed on deparaffinized heart sections using a Click-iT™ Plus TUNEL Assay Kits for In Situ Apoptosis Detection (Invitrogen, C10617), according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2024Quote: Single-molecule fluorescence in situ hybridization (smFISH) for miRNA detection in hippocampal neuron cultures was conducted using the QuantiGene ViewRNA miRNA Cell Assay Kit (Thermo Fisher, QVCM0001) following the manufacturer protocol with minor adjustments ...
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bioRxiv - Physiology 2024Quote: All real-time PCRs were carried out in 96-well plates using ChamQ SYBR qPCR Master Mix kit (Nanjing Vazyme Biotech Ltd., China) in the ABI Prism 7500 Sequence Detection System (Applied Biosystems, USA) at 95°C ...
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bioRxiv - Immunology 2021Quote: ... plate was incubated with HRP conjugated anti-human IgA (1:1000, Thermo Scientific) for 1hour in TBST with 1% non-fat dry milk ...
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bioRxiv - Immunology 2021Quote: ... plates were washed and developed with 1 Step TMB-Ultra (Thermo Fisher 34029). The reaction was stopped with 1M H2SO4 and plates were immediately read on FilterMax F3 (Molecular Devices) ...
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bioRxiv - Developmental Biology 2021Quote: ... 6-well plate in mTeSR media with 1:200 P/S (Gibco, 15140122) and 1:1000 Y-27632 (STEMCELL ...
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bioRxiv - Immunology 2022Quote: ... 1-2 million PBMCs were plated into 12 well plate (Nunc, Thermo Fisher) at 37°C and 5% CO2 and allowed to adhere for two hours in 1 mL of RPMI 1640 (Hyclone ...
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bioRxiv - Immunology 2022Quote: ... 1-2 million PBMCs were plated into 12 well plate (Nunc, Thermo Fisher) at 37°C and 5% CO2 and allowed to adhere for two hours in 1 mL of RPMI 1640 (Hyclone ...
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bioRxiv - Bioengineering 2021Quote: ... 1% penicillin/streptomycin (P/S) on the 96-well plates (Thermo Fisher Scientific). Samples were serially diluted 10-fold from 10−1 to 10−8 in the MEM containing 5% FBS and 1% P/S ...
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bioRxiv - Cancer Biology 2019Quote: ... Culture plates were coated with 10 μg ml-1 laminin (Invitrogen, 23017-015) for 2 h before use ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the plate was incubated with HRP-conjugated streptavidin (Thermo Scientific; #21126; 1:5000) for 2h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... live embryos in 6-well plates were incubated in YO-PRO-1 (Invitrogen) staining solution (2 μM YO-PRO-1 in E3 medium ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Control samples on each analyzed plate included calf serum diluted 1:500 (Gibco), blocking buffer only ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed and 1-Step Ultra-TMB ELISA Substrate Solution (Thermo Scientific) was added to develop the plates ...