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Citations for Thermo Fisher :
1701 - 1750 of 10000+ citations for 6 CHLORO 3 NITROPYRIDINE 2 CARBOXAMIDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... IL-6 (Thermo Fisher Scientific, #P620), P16 (SCBT ...
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bioRxiv - Immunology 2024Quote: ... in 6-well plates (Thermo Scientific) at 1.5x106 cells per well for 1 h at 37°C in warm RPMI-1640 medium (GIBCO) ...
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bioRxiv - Cell Biology 2024Quote: ... 6) glucose-free DMEM (Gibco, 11966025) for 24 hr ...
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bioRxiv - Molecular Biology 2024Quote: ... and 6 mM magnesium chloride (Invitrogen) was added ...
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bioRxiv - Molecular Biology 2024Quote: ... and 6 mM magnesium chloride (Invitrogen) was assembled ...
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bioRxiv - Cell Biology 2020Quote: ... EndoB1 siRNA 5’-UGUUUAUACGACUUGGAGCUU-3’ and 3’-AAGCUCCAAGUCGUAUAAACA-5’ (Invitrogen), control siRNA (Ambion) ...
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bioRxiv - Microbiology 2021Quote: ... FluoZin-3 (FluoZin™-3, AM, cell permeant, Thermo Fisher) was added at 1 mM ...
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bioRxiv - Immunology 2020Quote: ... and 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (Thermo Fisher Scientific). The activated beads were washed three times with 50 mM MES pH 5.0 and added to SARS-CoV-2 S protein which was diluted in 50 mM MES pH 5.0 ...
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bioRxiv - Immunology 2022Quote: ... and 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (Thermo Fisher Scientific) and incubated for 30 min on a rotator at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... mounting 3 dpf embryos in 3% methylcellulose (Thermo Scientific, 258111000). After imaging ...
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bioRxiv - Cell Biology 2023Quote: ... siPALS1 (5’-UUCCUUAUGAUGAACUGGCtt-3’) and siPATJ (5’-CCAGAUACUCACACUUCAGtt-3’, Ambion), siARP2/3 (5’- GGAUUCCAUUGUGCAUCAAtt-3’ ...
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bioRxiv - Biochemistry 2023Quote: ... DiIC12(3) (1,1’-Didodecyl-3,3,3’,3’-Tetramethylindocarbocyanine Perchlorate) (Invitrogen, D383) was used ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4’,6-diamidino-2-phenylindole (DAPI) (00-4959-52, Invitrogen, Thermo Fisher Scientific, MA, USA) as counterstaining.
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bioRxiv - Cancer Biology 2020Quote: ... MiRNAs or vectors were transfected in the amount of 2 μg for 6-well plate into indicated cells by Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific, Waltham, USA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... the coverslips were placed upside down on a glass slide with slow fade mounting medium containing 4’,6-diamidino-2- phenylindole (DAPI, Life Technologies, Carlsbad, CA, USA) and kept at 4 °C until observation ...
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bioRxiv - Microbiology 2020Quote: ... were acid digested on a hot plate using 6 mL Primar Plus grade HNO3 (68%) and 2 mL H2O2 (Thermo Fisher Scientific, Loughborough, UK). Samples were diluted with Milli-Q water (18.2 MΩ cm ...
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bioRxiv - Developmental Biology 2022Quote: ... and incubated with Alexa-Flour-488 or 555 secondary antibodies (1:1000) along with 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, Camarillo, CA, 1:1000) for 1 hr at room temperature (RT) ...
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bioRxiv - Microbiology 2021Quote: ... Nuclei were counterstained with Hoechst 33342 DNA dye NucBlue® Live ReadyProbes® reagent for live cell imaging or 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI) and SYTO® 60 fluorescent nucleic acid stain for fixed samples (Invitrogen, Molecular Probes, Germany).
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bioRxiv - Biochemistry 2020Quote: ... Coverslips were mounted and cells were counterstained on the glass slides using ProLong Gold with DAPI (4’, 6-diamidino-2-phenylindole) (Molecular Probes, Eugene, OR, US). Images were collected using an Axio Plan2/AxioCam microscope and image processing was performed with MrC5/Axiovision software (Zeiss ...
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bioRxiv - Immunology 2021Quote: ... bacteria were stained with 2µg/µL of 4’,6-diamidino-2-phenylindole (DAPI) and mounted in ProLong Diamond Antifade (Life Technologies, Eugene, OR, USA; P36961). Image acquisition was performed using a Leica confocal microscope (Leica ...
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bioRxiv - Cancer Biology 2022Quote: ... To quantitate cell migration 6 fields of view per membrane were photographed with EVOS™ FL Auto 2 Imaging System(Thermo Scientific™ Invitrogen™), followed by quantification by ImageJ v1.53q (National Institutes of Health ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were stained with DAPI (4’,6-diamidino-2-phenylindole) and cover-slipped with ProLong Glass Antifade Mountant (P36984, Thermo Scientific, Waltham MA, USA). All images were acquired on a Keyence-BZ800 microscope ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... After several wash and centrifugations, nuclei were suspended in a solution (phosphate=buffered saline containing 1% 4’, 6-diamidino-2-phenylindole (DAPI) (Molecular Probes, Eugene, OR, USA), and nuclei density was determined in a hemocytometer ...
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bioRxiv - Developmental Biology 2022Quote: ... 3% FBS (Gibco), 1% MEM non-essential amino acids (Gibco) ...
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bioRxiv - Genomics 2019Quote: ... 3 (Applied Biosystems).
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bioRxiv - Microbiology 2021Quote: ... DiOC2(3) (Invitrogen) was added to a final concentration of 30µM or DiSC3(5 ...
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bioRxiv - Cancer Biology 2022Quote: ... 3% FBS (Invitrogen), 20 ng ml−1 EGF (Peprotech ...
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bioRxiv - Cell Biology 2020Quote: ... SUMO2/3 (Invitrogen), β-catenin (BD Transduction Laboratories) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 3 (Applied Biosystems). We assembled forward and reverse reads using the Geneious (https://www.geneious.com ...
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bioRxiv - Neuroscience 2023Quote: ... 3% FBS (Gibco), 0.1mM MEM-NEAA (Gibco) ...
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bioRxiv - Immunology 2024Quote: ... TOPRO-3 (Invitrogen) was added to the staining buffer at a dilution of 1:10000 ...
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bioRxiv - Cancer Biology 2021Quote: ... each sample was injected onto an EASY-Spray PepMap C18 column (75 mm id 3 25 cm, 2 mm particle size) (Thermo Scientific) and separated over a 2-h method ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were injected and concentrated on a trap column (PepMap100 C18, 3 µm, 100 Å, 75 µm i.d. x 2 cm, Thermo Scientific) equilibrated with 0.05% TFA ...
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bioRxiv - Molecular Biology 2021Quote: ... Peptides were injected onto an Acclaim PepMap100 C18 trap column (75 μm i.d., 2 cm long, 3 μm, 100 Å, Thermo Scientific) and further separated on an Acclaim PepMap100 C18 analytical column (75 μm i.d. ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed 2-3 times (200 rpm, 5 min at 4°C) in eBioscience™ Permeabilization buffer (250 µl/well) (Invitrogen) and resuspended in eBioscience™ Fixation/Permeabilization solution (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... Peptide mixtures were loaded on a C18 Acclaim PepMap100 trap-column (75 μm ID x 2 cm, 3 μm, 100Å, ThermoFisher Scientific) for 3.5 minutes at 5 μL/min with 2% ACN (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... 2-3 ml of logarithmically growing yeast cells in DOA media were added to a 35 mm FluoroDish (Fisher Scientific; 15199112) which had been pre-incubated at 30°C with Concanavalin A (Sigma Aldrich ...
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bioRxiv - Cell Biology 2019Quote: Bone marrow was obtained from 2-3-month-old mice and cultured with 100 ng/ml of M-CSF (Prospec Bio) in αMEM (Gibco Invitrogen) supplemented with 10% FCS for three days ...
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bioRxiv - Biophysics 2019Quote: ... pieces of tissue containing 2-3 tracheal segments were dissected in ice-cold Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 (ThermoFisher, DMEM/F12). Tissue sections were sliced open along the proximal-distal axis from the dorsal side and mounted onto 35mm cell imaging dishes (Mat-Tek) ...
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bioRxiv - Biophysics 2021Quote: ... between 0.5-2 mg of individual histones were combined and dialyzed into 2 M NaCl (10 K MWCO, 3-12 mL Slide-A-Lyzer Dialysis Cassettes, ThermoFisher Scientific). After dialysis the histones in 2 M NaCl were concentrated to a volume of 1 mL and purified using an Superdex 200 column (GE Healthcare ...
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bioRxiv - Systems Biology 2021Quote: ... Peptide mixtures were injected in 0.1% TFA on a C18 Acclaim PepMap100 trap-column (75 μm ID × 2 cm, 3 μm, 100Å, Thermo Fisher Scientific) for 3 min at 5 μL/min with 2% ACN ...
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bioRxiv - Biochemistry 2020Quote: ... targeting the TLR3 gene (5’-CCUGAUGAUCUUCCCUCUAACAUAA-3’) and Stealth RNAi siRNA negative control med GC Duplex #2 were obtained from Thermo Fisher Scientific ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: HeLa cell pellets (∼2×107) or MCF7 cell pellets (∼4×107) were lysed with 3 mL Mammalian Protein Extraction Reagent (Thermo Scientific), supplemented with 2X HALT protease inhibitor (Thermo Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... we added a 1 mL of the following mixture to the culture media and incubated the cells at 25°C incubator for 1—3 hours: 5 μM Fura-2 AM (F-1201, Life Technologies), 250 μM probenecid (162-26112 ...
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bioRxiv - Neuroscience 2020Quote: Cortical primary neurons from newborn triple neurexin-1/2/3 conditional KO mice13 were seeded on Matrigel-coated coverslips in a 24-well plate in MEM medium (ThermoFisher Scientific). On DIV2 ...
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bioRxiv - Biophysics 2021Quote: α2-GFP or α2:β at 1:3 ratio was transfected into HEK293T cells on collagen coated glass bottom dishes using lipofectamine 3000 (Invitrogen) for expression of α2 GlyR or α2β GlyR ...
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bioRxiv - Neuroscience 2021Quote: 2.5 μg peptides were pre-concentrated with a flow of 3 μL/min for 10 min using a C18 trap column (Acclaim PepMap100, 100 μm x 2 cm, Thermo Scientific) and then loaded onto a 50 cm long C18 column (75 μm ID ...
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bioRxiv - Systems Biology 2020Quote: ... 1 μg peptides from each fraction was loaded onto trap column nanoViper 2 cm (3 μm C18 Aq) (Thermo Fisher Scientific). Peptide separation was carried out using EASY-Spray C18 analytical column (15 cm ...
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bioRxiv - Plant Biology 2021Quote: ... The leaf discs were then counterstained by immersing them in 0.02 µg/mL DAPI in water for 3 min and rinsed 2× in water before imaging them using a fluorescence microscope (Evos, Thermo Fisher).
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bioRxiv - Microbiology 2021Quote: ... The peptides were loaded onto an Acclaim PepMap 100 (ID 75μm x 2 cm, 3 μm, 100 Å) pre-column and separated on an EASY-Spray column (Thermo Scientific; ID 75 μm × 25 cm ...