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Citations for Thermo Fisher :
1851 - 1900 of 10000+ citations for 6 CHLORO 3 NITROPYRIDINE 2 CARBOXAMIDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... we used 15 μL of a mixture of 4’,6-diamidin-2-phenylindole (4 μg mL−1) in SlowFade Gold Antifade Mounting medium (both Thermo Fisher Scientific, Waltham, MA, USA). All solutions and buffers for virus-targeted genomeFISH experiments were prepared with molecular grade water (Carl Roth ...
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bioRxiv - Cell Biology 2022Quote: ... Cells nuclei were counter-stained using 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI) mounting medium (ProLong™ Diamond Antifade Mountant with DAPI, Thermo Fisher Scientific, Warsaw, Poland). Preparations were observed using the confocal microscope under magnification 630× and processed with Fiji software (ImageJ 1.52n ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4’,6-diamidino-2-phenylindole (DAPI) (00-4959-52, Invitrogen, Thermo Fisher Scientific, MA, USA) as counterstaining.
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bioRxiv - Plant Biology 2021Quote: ... two SlP4H3 OEX lines (#1, #2) and three SlP4H3 RNAi lines (#1, #6 and #7) by using the TRI reagent (Thermo Fisher Scientific, Waltham, MA, USA). Reverse transcription of approximately 1 μg of total RNA was performed with Superscript II® Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: Lines with defective stomatal responses to at least one stimulus were subjected to sequencing of “usual suspects” either by NGS-based sequencing of PCR amplicons obtained with the use of gene-specific primers (Supplementary Data Set 2) and Phusion DNA polymerase (ThermoFisher Scientific; ROUND 1 to 6), or whole-genome sequencing (ROUND 7 and 8) ...
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bioRxiv - Cell Biology 2021Quote: ... THP-1 cells (1.5×105 cells/mL) were labeled with a fluorescent dye 2’,7’-bis(carboxyethyl)-5 (6)-carboxyfluorescein-AM (Thermo Fisher Scientific B1150; 1 mg/mL) in serum-free RPMI medium (Thermo Fisher Scientific 11875093 ...
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bioRxiv - Molecular Biology 2022Quote: A concentration of 6 × 103 cells was loaded with 5 mM 4-amino-5-methylamino-2’,7’- difluorofluorescein diacetate (DAF-FM, Molecular Probes, Thermo Fisher, Sao Paulo, Brazil) after 72 h of treatment ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Several donor colonies and one recipient colony were independently inoculated in 2 mL of LB in 15-mL tubes and cultured for 6 hours (37°C and 250 rpm, Thermo Scientific™ MaxQ™ 8000). Cultures were centrifuged (15 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... was cultured in RPMI with 5 % heat inactivated human serum (HS) and 2 ng/mL interleukin (IL)-6 (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). In vitro experiments with KJON cells were performed with 2 % HS in RPMI and IL-6 (1 ng/mL) ...
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bioRxiv - Biochemistry 2021Quote: ... Enzyme assays were conducted in 0.5 mL containing 50 mM 2-(N-morpholino)ethanesulfonic acid (pH 6) and various concentrations of soluble starch (Acros Organics, No. AC424495000, Morris Plains, NJ). The assays also included 100 mM KCl ...
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFUSE-vector (Invitrogen, Thermo Fischer Scientific, MA, USA). The human ACE2 was expressed by essentially the same protocol used for the COVID-19 RBD ...
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bioRxiv - Cancer Biology 2022Quote: ... To quantitate cell migration 6 fields of view per membrane were photographed with EVOS™ FL Auto 2 Imaging System(Thermo Scientific™ Invitrogen™), followed by quantification by ImageJ v1.53q (National Institutes of Health ...
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bioRxiv - Neuroscience 2024Quote: ... then separated on a reverse-phase nano-spray column (2 μm, 75 mm i.d. × 50 cm, 100 Å, Thermo Fisher Scientific Cat# 16-494-6) using gradient elution ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Nvsix3/6:venus was generated by subcloning the Nvsix3/6 coding sequence into pENTR/D TOPO (ThermoFisher Scientific) using published primers previously used to PCR amplify Nvsix3/6 and synthesize Nvsix3/6 mRNA 47 ...
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bioRxiv - Microbiology 2021Quote: ... thaliana seedlings cultured in 6 ml liquid MSgg of each well of a 6-well microplate (Thermo Scientific). 8 seedlings were put in each well ...
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bioRxiv - Microbiology 2020Quote: ... Cells in antibiotic free media (8×105/6-well) were transfected 6 hours post infection with 2μg plasmid and 6μl Lipofectamine 2000 (Invitrogen) per well diluted in Opti-MEM (Invitrogen).
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bioRxiv - Synthetic Biology 2024Quote: ... Jurkat cells and NALM-6 expressing firefly luciferase- GFP (NALM-6) were cultured in RPMI 1640 (Thermo Fisher) supplemented with 10% FBS and 100 units/mL penicillin and streptomycin ...
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bioRxiv - Cell Biology 2020Quote: ... and TagRFP-T-VAPB(1-218)-eMagB or TagRFP-T-eMagB-PHOSBP (prey) and iRFP-P4C at a 3:2:1 ratio in OptiMEM-I (Thermo Fisher Scientific) (1:4 DNA ...
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bioRxiv - Cell Biology 2020Quote: Total cellular mRNA was isolated from cells grown in 2-D and 3-D culture conditions using the GeneJET RNA purification kit (Thermo Fisher Scientific). For analysis of germ layer markers ...
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bioRxiv - Cell Biology 2020Quote: An aliquot of each sample equivalent to 3 μg was loaded onto a trap column (Acclaim PepMap 100 pre-column, 75 μm × 2 cm, C18, 3 μm, 100 Å, Thermo Scientific) connected to an analytical column (Acclaim PepMap RSLC column C18 2 μm ...
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bioRxiv - Biochemistry 2022Quote: ... The trap column used for the nano HPLC was a 2 cm × 75 μm capillary column packed with 3 μm C18-silica particles (Thermo Fisher Scientific) and the separation column was a 12.5 cm × 75 μm capillary column packed with 3 μm C18-silica particles (Nikkyo Technos Co. ...
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bioRxiv - Molecular Biology 2021Quote: ... Peptide mixtures were loaded on a C18 Acclaim PepMap100 trap-column (75 μm ID x 2 cm, 3 μm, 100Å, Thermo Fisher Scientific) for 3.5 minutes at 5 μL/min with 2% Acetonitrile MS grade (Sigma Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... RNA interference was done by transfecting 100 nM siRNA (TPD54 1: GUCCUACCUGUUACGCAAU, TPD54 2:CUCACGUUUGUAGAUGAAA, TPD54 3:CAUGUUAGCCCAUCAGAAU) with Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... Peptides were trapped on a C18 reversed-phase trap column (2 cm x 75 μm ID; Acclaim PepMap trap column packed with 3 μm beads, Thermo Fisher Scientific) and separated on a 25 cm C18 reversed-phase analytical column (25 cm x 75 μm ID ...
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bioRxiv - Biochemistry 2019Quote: ... The peptides were loaded on a reversed-phase trap column (75 μm × 2 cm, 3 μm, C18, 100 Å, Thermo Scientific, Germany), and then the eluent was transferred to a reversed-phase analytical column (50 μm × 150 mm ...
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bioRxiv - Cancer Biology 2020Quote: ... were concentrated and desalted on a trapping pre-column (Acclaim PepMap C18, 75 µm × 2 cm nanoViper, 3 µm, 100 Å, Thermo Scientific), using 0.2% formic acid at a flow rate of 5 µl/min ...
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bioRxiv - Neuroscience 2020Quote: ... and then 12 µg from each was injected into a 75 μm × 2 cm nanoViper C18 Acclaim PepMap100 precolumn (3 μm particle size, 100 Å pore size; P/N 164705; Thermo Scientific). Peptides were separated at a flow rate of 250 nl/min and eluted from the column over a 5 hours gradient starting with 0.1% FA in ACN (5-30% ACN from 0 to 280min followed by 10min ramping up to 80% ACN ...
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bioRxiv - Biochemistry 2020Quote: ... Peptide sample (2 μL) was injected onto an Acclaim PepMap 1000 trap column (75 μm × 2 cm, nanoViper C18 3 μm, 100Å, Thermo Fisher Scientific) which was heated to 40 °C in a chamber which was connected to a C18 reverse-phase Aurora UHPLC Emitter Column with nano Zero & Captive Spray Insert (75 μm × 25 cm ...
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bioRxiv - Biochemistry 2020Quote: ... N-((6-(2,4-DNP)Amino)Hexanoyl)-1-(BODIPY™ FL C5)-2-Hexyl-Sn-Glycero-3-Phosphoethanolamine (PED-A1) and BODIPY™ FL C5 were purchased from Thermofisher scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed with PBS (3 x 10 min) and incubated 2 hours with the appropriate secondary antibodies (Invitrogen, CA, USA) at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... washed 3 times with blocking buffer and incubated for 2 h with Alexa 647 anti-rabbit (1/250, A21247, Thermo Fisher Scientific). After washing the cells 3 times with PBS ...
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bioRxiv - Biochemistry 2021Quote: ... approximately 1 pmol injected onto a Acclaim PepMap 100 guard column (75 μm×2 cm C18, 3 μm particles, 100 Å; Thermo Scientific) and separated on a 50 cm PepMap RSLC C18 (75 μm×50 cm ...
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bioRxiv - Neuroscience 2020Quote: Cells were electroporated with 2 μg of donor construct and 3 μg of px330-Cas9 plasmid using a Neon Transfection System (Thermo Fisher Scientific) and the following settings ...
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bioRxiv - Biophysics 2020Quote: ... We grew HL-60 cells to approximately 1 × 106 cells/ml and passaged the cells every 2-3 days in Roswell Park Memorial Institute medium (RPMI-1640, Thermo Fisher Scientific) supplemented with L-glutamine and 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... BV-2 immortalized microglial cells (Blasi et al. 1990) (passage 3 to 8) cultured in macrophage serum-free medium (ThermoFisher 12065-074) were used ...
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bioRxiv - Cell Biology 2021Quote: ... were separated using NuPAGE Bis-Tris gels (4 – 12 %) with 3-(N-morpholino)propanesulfonic acid (MOPS) or 2-(N-morpholino)ethanesulfonic acid (MES) running buffer (Thermo Fisher Scientific) followed by transferring to polyvinylidene difluoride (PVDF ...
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bioRxiv - Biochemistry 2022Quote: The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Orbitrap Exploris 480 for replicate 2, and Q-Exactive HF for replicate 3, Thermo Fisher Scientific). Peptides were sampled on a precolumn (300 μm × 5 mm PepMap C18 ...
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bioRxiv - Immunology 2022Quote: ... Peptide lysates were injected on a trapping column (Acclaim PepMap 100 C18, 3 μm, nanoViper, 75 μm x 2 cm, Thermo Fisher Scientific) with 5 μl/min ...
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bioRxiv - Biochemistry 2021Quote: ... spectra were recorded on a Thermo-Finnigan Surveyor equipped with a Satisfaction RP18AB C18 3 μm (15×2 mm) (Cluseau) and coupled to an ESI LCQ Advantage (Thermo Fisher Scientific). The running mixture was subjected to filtering on a Microcon filter unit of 10 kDa (15 min ...
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bioRxiv - Microbiology 2021Quote: ... using an Acclaim PepMap100 pre-column (C18, 3 μm-100 Å) and a PepMap RSLC (C18, 2 μm, 50 μm-15 cm) both from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... peptides were loaded onto a trap column (PepMap C18 trap column 75 µm × 2 cm, 3 µm, particle size, 100 Å pore size; ThermoFisher Scientific) at 5 μL/min for 3 min before switching the pre-column in line with the analytical column (PepMap C18 analytical column 75 μm × 50 cm ...
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bioRxiv - Systems Biology 2019Quote: ... equipped with a reverse phase trap column (Acclaim PepMap 100, 75 µm x 2 cm, 3 µm, 100 Å; Thermo Fisher Scientific) and 50 cm analytical column (EASY-Spray ...
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bioRxiv - Microbiology 2020Quote: ... incubating with primary antibodies (rabbit anti-β-actin, 1:1000, Thermo Fisher Scientific, PA1-183; mouse anti-claudin-2, 3:500, Invitrogen, 325600 ...
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bioRxiv - Cancer Biology 2020Quote: ... LC separation was executed with a trap column (C18, 75 μm*2 cm, 3 μm) (cat No. 164946, Thermo Fisher Scientific, Germany) and an analytical C18 column (C18 ...
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bioRxiv - Bioengineering 2019Quote: ... medium was switched to a B27-based Retinal differentiation medium (BRDM) (DMEM/F12 (3:1) with 2% B27 (w/o vitamin A, ThermoFisher Scientific, USA), 1x NEAA and 1x AA) ...
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bioRxiv - Microbiology 2020Quote: ... were performed on an Ultimate 3000 RSLC nano instrument coupled to either a QExactive Plus (M#1, M#3) or an HF mass spectrometer (M#2; Thermo Fisher Scientific) as described previously.24 Tryptic peptides were trapped for 4 min on an Acclaim Pep Map 100 column (2 cm × 75 μm ...
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bioRxiv - Immunology 2020Quote: ... D-PBS (D-PBS without Ca++ and Mg++) supplemented with 2% FBS and 3 mM cell culture grade EDTA (Life Technologies; Thermofisher) prior to monocyte isolation ...
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bioRxiv - Genomics 2021Quote: Human brain tissues were manually dissected into small 2-3 mm3 pieces and immersed in homogenization buffer (HBSS (Life Technologies, 14175-095), 1% bovine serum albumin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: N2A cells were transfected as described above with BicD2-KIF tail fusions and MBNL-GFP proteins at a ratio of 2:3 in 4-well chamber slides (Thermo Fisher, 154526). 24 hours after transfection ...
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bioRxiv - Biochemistry 2022Quote: ... Peptides were loaded onto a trap column (Dionex-C18 trap column 75 μm × 2 cm, 3 μm, particle size, 100 Å pore size; ThermoFisher Scientific) at 5 μl/min before switching the pre-column in line with the analytical column (Dionex-C18 analytical column 75 μm × 50 cm ...