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Citations for Thermo Fisher :
1551 - 1600 of 10000+ citations for 6 CHLORO 3 NITROPYRIDINE 2 CARBOXAMIDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... The cells were rinsed three times with 1X PBS and coated with ProLong Gold antifade mounting medium with DAPI (4’,6-diamidino-2-phenylindole, 100 ng/ml) (Life Technologies, P-36931) on slides ...
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bioRxiv - Bioengineering 2023Quote: ... These slices were then fixed in 4’,6-diamidino-2-phenylindole (DAPI) mounting media (Fluoromount-G® with DAPI, Thermo Fisher Scientific, USA), and washed twice with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were then stained with 4′,6-diamidino-2-phenylindole (DAPI) to stain cell nuclei and mounted with Immu-Mount mountant (Thermo Scientific, Cat# 9990402) onto microscope slides.
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bioRxiv - Neuroscience 2023Quote: ... Cells were transfected for 6 or 24 hours with Imp1-targeting siRNAs (siRNA #1: s155193, or siRNA #2: s155193. Both from Ambion, Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... and blocked for 45 min in 2% bovine serum albumin (BSA) in PBS prior to staining with DAPI (4=,6-diamidino-2-phenylindole) and mounted in Prolong Diamond antifade (Molecular Probes, Life Technologies). Images were captured using a Nikon Eclipse Ti confocal microscope (Nikon Instruments ...
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bioRxiv - Bioengineering 2023Quote: ... for 30 seconds at room temperature followed by 15 min staining with 1 µg/mL 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen, cat. no. D1306) before microscopic imaging ...
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bioRxiv - Bioengineering 2023Quote: ... The slides were thoroughly rinsed and sealed utilizing a SlowFade Gold antifade reagent with 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific) and coverslips.44 The stained slides were imaged at 4x magnification with an EVOS fluorescence imaging system.
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bioRxiv - Cell Biology 2024Quote: For nuclei and F-actin staining were visualized with 4’,6-diamidino-2-phenylindole (DAPI) (#D523, Dojindo; 1:1000 dilution) and phalloidin Alexa Fluor plus 405-conjugated (#A30104, Invitrogen; 1:400 dilution), phalloidin ATTO 565-conjugated (#94072 ...
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bioRxiv - Cell Biology 2020Quote: ... and then loaded with fluorescent Ca2+ probes (3 μM of fura-2 AM or 5.69 μM of Fluo-4 AM, Life Technologies) in HBSS for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... A Neuropixels probe (option 3 phase A) with 374 recording sites was briefly (∼2 minutes) dipped into the diI cell-labeling solution (ThermoFisher) to visualize probe tracks ...
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bioRxiv - Molecular Biology 2020Quote: ... medium from the basolateral compartment was renewed every 2–3 days and the apical surface was washed once a week with PBS (Invitrogen).
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bioRxiv - Neuroscience 2020Quote: ... third instar larvae were dissected in zero Ca2+ HL-3 solution at room temperature and stimulated with a HL-3 solution of 90 mM K+/2 mM Ca2+/4 μM FM4-64 (Invitrogen) for 5 min to load FM4-64 dye into the boutons ...
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bioRxiv - Cell Biology 2019Quote: Bone marrow was obtained from 2-3-month-old mice and cultured with 100 ng/ml of M-CSF (Prospec Bio) in αMEM (Gibco Invitrogen) supplemented with 10% FCS for three days ...
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bioRxiv - Genetics 2019Quote: ... Transfection was performed using 2 μg plasmid DNA and 3 μL Lipofectamine in 150 μL Opti-MEM I (ThermoFisher 31985062). At collection ...
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bioRxiv - Systems Biology 2019Quote: ... the media was aspirated and replaced with 100μL phenol-red free RPMI-1640 and 12mM 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium (MTT) (Thermo Fisher Scientific, #M6494) was added to the cells ...
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bioRxiv - Molecular Biology 2019Quote: ... 3-9) or 5 µl (IP: Figure 2A-2C and input: Figure 2-9) RNAse A/T1 (Thermo Scientific #EN0551) and incubation the beads at 37°C for 30 minutes ...
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bioRxiv - Biochemistry 2019Quote: ... Samples were desalted on a trap column (PepMap100 C18, 3 μm, 100 Å, 75 μm i.d. × 2 cm; Thermo Scientific) using a mobile phase of 0.05 % (v/v ...
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bioRxiv - Genetics 2020Quote: ... samples were diluted in Laemmli sample buffer and 2 μg of protein was separated on a NuPage 3–8% Tris-Acetate gel (Invitrogen). The gel was then stained with Coomassie Blue and digitalized.
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bioRxiv - Microbiology 2021Quote: ... The 5’ and 3’ fragments (2 μg of each) were ligated for 1 h at 16°C by T4 DNA ligase (Invitrogen) and purified by phenol-chloroform extraction ...
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bioRxiv - Genetics 2021Quote: 2-3 × 106 mESCs were transfected with 14.5 μg of TALE plasmid and 26 μL Lipofectamine 3000 Reagent (Invitrogen L3000015) and seeded onto 0.1% gelatin-coated 10 cm dish containing an autoclaved SuperFrost Plus Adhesion glass slide ...
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bioRxiv - Bioengineering 2020Quote: ... proteins and the Canadian clinical isolate of SARS-CoV-2 (V-2/Canada/VIDO-01/2027 Feb 2020) were mixed in fresh media supplemented with 3% fetal bovine serum (Gibco) and preincubated for 1 hour at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated overnight at 4°C in blocking solution (PBS, 2% normal goat serum, 3% BSA) and primary antibodies (mouse anti-HA, Invitrogen; rabbit anti-GFP, Invitrogen). Cells were then immunolabeled with Alexa-conjugated secondary antibodies (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed twice with PBS prior to loading with 3 μM of the Ca2+ indicator Fura-2 AM (Invitrogen) diluted in a modified Krebs-Ringer buffer [KRBH ...
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bioRxiv - Pathology 2021Quote: ... The tissue was homogenized with a Pasteur pipette in Growth medium [3 ml DMEM-F12 with 2% of B27 without vitamin A (Gibco), 20 ng.mL-1 Epidermal Growth Factor (EGF ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was produced from 2-3 g total RNA as described (Greenstein et al 2018) using a dT primer and Superscript IV (Invitrogen) reverse transcriptase ...
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bioRxiv - Cancer Biology 2020Quote: ... Total RNA was isolated from sorted 2-3×10^5 CD34+ cells using the mirVANA miRNA isolation kit (Thermo Fisher) and subsequently processed using the Small RNA Library Prep kit (Norgen Biotek) ...
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bioRxiv - Biophysics 2021Quote: ... slides were rinsed in PBS for 3 x 5 mins and stained with 1 %g/mL 4,6-diamino-2-phenylindole (DAPI - Molecular Probes) for 3 mins in the dark at RT ...
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bioRxiv - Cell Biology 2021Quote: ... cells were loaded with 3 μM Fura-2-AM and 0.01% (v/v) Pluronic F-129 (both from Molecular Probes) in DMEM medium at 37 °C for 20 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were treated with RNAScope H2O2 for 4 min at RT and subsequently washed 2 x 3 min with UltraPure Distilled Water (Invitrogen) at RT ...
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bioRxiv - Cancer Biology 2022Quote: mScarlet-fascin/fascin KD or mScarlet/fascin KD HeLa cells were plated with 2 mM CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher) in CellCarrier Ultra 384 well plates (PerkinElmer ...
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bioRxiv - Zoology 2022Quote: ... 2.3 × 105 Huh-7 cells were transfected with 2 µg of pSpCas9-BB-2A-GFP-sgPURA using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Cell toxicity due to the compound treatment was determined using the standard colorimetric MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... ionomycin (1 μg/ml) and monensin (2 μg/ml) for 3-4 hours at 37°C in complete IMDM medium (Gibco). Viability staining was performed using the fixable viability dye eFluor780 ...
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bioRxiv - Microbiology 2020Quote: ... Parasitemia was monitored daily by flow cytometry by diluting 10 μl of each parasite culture well from 2-3 biological replicates into 200μl of 1.0 μg/ml acridine orange (Invitrogen Life Technologies A3568) in phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2021Quote: ... and 2 µL of total RNA was subjected to One-Step RT-qPCR using Applied Biosystems QuantStudio 3 (ThermoFisher Scientific), with the following cycling conditions ...
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bioRxiv - Immunology 2022Quote: The uptake and processing of CSF-derived protein by antigen-presenting cells outside the CNS was evaluated by injecting 3 μl of DQ-OVA (2 mg/ml, Invitrogen) into the CSF of chronically infected C57BL/6 mice by i.c.m ...
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bioRxiv - Bioengineering 2022Quote: ... The medium was replaced every 2-3 days and cells were split once they reached 75-80% confluence using TrypLE Express (Gibco). LNCaP and U87 cells were gifted by Professor Lisa Butler and Professor Stuart Pitson ...
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bioRxiv - Molecular Biology 2019Quote: ... 3’-linked footprints were denatured at 80°C for 2 min and purified on 10% TBE-Urea polyacrylamide gels (Invitrogen) in 1x TBE (Ambion ...
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bioRxiv - Molecular Biology 2020Quote: ... Per2Luc mice and littermate wild-type control mice were euthanized by isoflurane 2-3 hours before lights off (ZT 9–ZT10) and tissues were immediately removed into ice-cold HBSS (Gibco). Coronal brain slices containing SCN (400-µm thickness ...
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bioRxiv - Bioengineering 2021Quote: ... scaffold sequences and 3’ extension sequences were amplified with indicated primers (Supplementary table 2) using Phusion master mix (ThermoFisher Scientifc) or Q5 High-Fidelity enzyme (NewEnglandBioLabs) ...
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bioRxiv - Immunology 2019Quote: ... isolated lungs were cut into 2-3 mm2 sections and resuspended in 5 mL digestion solution of Roswell Park Memorial Institute (RPMI) 1640 Medium (Gibco) supplemented with 5 mM GlutaMax (Gibco) ...
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bioRxiv - Genomics 2021Quote: ... assembly was performed by mixing 2-3 sgRNAs (a total of 120 pmol) with 8.5 μg recombinant Cas9 (Invitrogen A36499). The resulting RNP mix was electroporated into 0.3×106 MV411 cells using a Lonza 4D Nucleofector ...
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bioRxiv - Cell Biology 2021Quote: ... the media was replaced entirely with fresh retinal differentiation media (RDM) (DMEM:F12 3:1, 2% B27 supplement, MEM NEAA, 1× antibiotic, antimycotic (Thermo Fisher) and 1× GlutaMAX)) ...
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bioRxiv - Microbiology 2021Quote: ... and cDNAs were synthesized using SARS-CoV-2 nucleocapsid (N) reverse primer N660R (5’-AGCAAGAGCAGCATCACCGCCATTGCCAGC-3’) and M-MLV reverse transcriptase (Invitrogen). Then ...
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bioRxiv - Genomics 2021Quote: ... assembly was performed by mixing 2-3 sgRNAs (a total of 120 pmol) with 8.5 μg recombinant Cas9 (Invitrogen A36499). The resulting RNP mix was electroporated into 0.3E6 MV411 cells using a Lonza 4D Nucleofector ...
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bioRxiv - Cell Biology 2020Quote: ... the media was changed to retinal differentiation medium (RDM;DMEM:F12 3:1, 2% B27 supplement, MEM NEAA, 1× antibiotic, antimycotic (Thermo Fisher) and 1× GlutaMAX) ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-ZO-2 pAb (38-9100) and rabbit anti-ZO-3 pAb (36-4100) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Cultures were regularly monitored for bacterial contamination by checking for bacterial growth in Zobell marine broth 2216 (HiMedia) (71) in addition to filtering 2-3 mL of exponential-phase growing culture and using Sybr Green I (Invitrogen) staining and epifluorescence microscopy (Nikon Eclipse 80i ...
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bioRxiv - Molecular Biology 2021Quote: ... For cell collection and/amplification ESCs were trypsinized for 2 to 3 minutes with trypsin-EDTA (Invitrogen, Cat#25200-072) and the digestion was stopped by the addition of pre-warmed 15% FCS ...
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bioRxiv - Immunology 2020Quote: ... 2 and 3 cDNA were amplified by real-time PCR using the SYBR Green PCR kit (Thermo Scientific, Braunschweig, Germany). The primer sequences used for the real-time PCR were listed in Table 1.