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Citations for Thermo Fisher :
1651 - 1700 of 10000+ citations for Pig Histone H3.3 H3F3A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Cells were then washed and exposed to anti-guinea pig or anti-mouse AF405- or AF488-conjugated secondary antibodies (1:500, Thermo Fisher Scientific) for one hour ...
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bioRxiv - Neuroscience 2023Quote: ... Alexa Fluor conjugated secondary antibodies (Alexa Fluor 488 conjugated goat anti-guinea pig, and goat anti-rabbit were from Invitrogen, MA, USA) were used at 1:500.
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bioRxiv - Genetics 2023Quote: Total RNA was extracted from tubulin-GAL4 > PIG-A RNAi (Bloomington Drosophila Stock Center: 62696) and control larvae using TRIzol Reagent (ThermoFisher Cat #15596026) followed by Direct-zol RNA Miniprep with DNAse step (Zymo Research R2051) ...
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bioRxiv - Genetics 2023Quote: A total of 1,618 French Large White pigs were genotyped using the Affymetrix PorcineWens55K SNP chip (Affymetrix, Santa Clara, CA, United States) in our previous study (Ding et al ...
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bioRxiv - Genomics 2021Quote: Histone samples were analyzed by nanoLC-MS/MS with an Easy-nanoLC coupled to an Orbitrap Fusion mass spectrometer (Thermo Fisher Scientific). The column was packed in-house using reverse-phase 75 µm ID × 17 cm Reprosil-Pur C18-AQ (3 µm ...
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bioRxiv - Cell Biology 2022Quote: ... incubated overnight with primary antibodies (1:5000 α-TNY1, 1:10,000 Roche Rat-anti-HA, high affinity 3F10, or 1:50,000 Invitrogen α-Histone H3) in 5% non-fat dry milk ...
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bioRxiv - Genomics 2022Quote: ... sections were immunostained for acetyl-Histone H3 (Lys9) rabbit antibody (Ac-H3K9, MA5-11195, 1:400, Thermo Fisher Scientific Inc., Waltham, MA) and fluorescently labeled with goat anti-rabbit ...
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bioRxiv - Biochemistry 2023Quote: ... Nucleosomes were reconstituted by dialyzing a stoichiometric mixture of histones and DNA in a dialysis device (Slide-A-Lyzer MINI Dialysis Device, 7K MWCO, Thermo Fisher Scientific) against 1X TE (pH 8.0 ...
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bioRxiv - Genomics 2023Quote: ... Histone samples were analyzed by nanoLC-MS/MS with a Dionex-nanoLC coupled to an Orbitrap Fusion mass spectrometer (Thermo Fisher Scientific). The column was packed in-house using reverse-phase 75 µm ID × 17 cm Reprosil-Pur C18-AQ (3 µm ...
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bioRxiv - Genetics 2023Quote: Analysis of digested histones was done on an Easy-nLC 1200 system coupled to a QExactive HF-X mass spectrometer (both Thermo Fisher Scientific) as described in Werner et al ...
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bioRxiv - Genetics 2024Quote: ... The digested and derivatized histone peptides were diluted in 0.1% TFA and injected to nLC-QqQ MS (Dionex nanoLC and a ThermoFisher Scientific TSQ Quantum). Peptides were first loaded to a trapping column (2 cm × 150 μm ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and incubated 1 hour at RT with secondary antibodies diluted in blocking solution as follows: anti-guinea pig Dylight 488 (Thermo Scientific, SA5-10094) (0.5 μg/mL ...
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bioRxiv - Immunology 2019Quote: ... then stained with secondary antibodies (donkey anti-rabbit DyLight 488, Abcam, 1:500; goat anti-guinea pig, Sigma, 1:500; anti-chicken Alexa 488, Life Technologies, 1:1000). Sections were mounted in VectaShield (Vector Labs ...
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bioRxiv - Neuroscience 2019Quote: ... cells were fixed with 4 % paraformaldehyde and stained for doublecortin (guinea pig anti-DCX IgG, 1:400, Merck Millipore followed by Alexa 488 anti-mouse, 1:500, ThermoFisher Scientific and DAPI). Images were obtained using a scanning confocal inverted microscope (TCS ...
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bioRxiv - Developmental Biology 2020Quote: ... and immunostained with either Hairless (guinea pig 1:500, Annett and Dieter) and β-gal or V5 (mouse 1:500, Invitrogen R960-25) and β-gal as indicated ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated with secondary antibodies Alexa Fluor anti-guinea pig 555 and Alexa Fluor anti-mouse 488 (Thermo Fisher Scientific; 1:400) at RT on a rotating platform for 2 h ...
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bioRxiv - Neuroscience 2021Quote: ... and slides were incubated for 90 min at room temperature with Goat secondary antibody to guinea pig IgG - Alexa Fluor 568 (1:200, Invitrogen, Waltham, MA, USA), goat secondary antibody to Rabbit IgG - H&L (Cy5 ...
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bioRxiv - Neuroscience 2021Quote: ... The following day the slices were washed three times for 10 minutes in PBS and incubated with secondary antibodies overnight at 4°C (Goat-anti-Guinea-pig-Alexa Fluor 488, Abcam ab150185, 1:500 and Goat-anti-mouse Alexa Fluor 647, Invitrogen A32728, 1:500). Slices were mounted with a #1.5 coverslip using ProLong™ Diamond Antifade Mountant without DAPI (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies used in this project were donkey anti-guinea pig 488 (Jackson, Cat#: 706-545-148, 1:500) and donkey anti-rabbit 594 (ThermoFisher, Cat#: A-21207). DAPI was added with secondary antibody (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... slices were rinsed 6×10 minutes and incubated with an AlexaFluor488-conjugated goat-anti-guinea pig IgG (1:200, Invitrogen A-11073, RRID:AB_2534117) for 4 hours ...
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bioRxiv - Neuroscience 2023Quote: ... We visualized immunoreactive complexes using anti-rabbit or anti-guinea pig secondary antibodies conjugated to Alexa-488 and -647 fluorophores (1:1000 for both, Invitrogen, Waltham, MA, USA).
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bioRxiv - Immunology 2023Quote: ... were pre-wet with 35% ethanol in sterile MiliQ water for 1 min and coated with 5 μg/mL of monoclonal mouse anti-pig IFN-γ antibody (clone P2F6, Thermo Fisher Scientific) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... SWANT GP72 1:1000) followed by Alexa Fluor 568 goat anti-guinea pig secondary antibody (No: A-11075, Thermo Fisher Scientific, 1:500). Antibodies and dilution concentrations were previously reported67–69 ...
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bioRxiv - Neuroscience 2023Quote: ... the cells incubated with secondary antibodies in blocking solution for 1h at RT in the dark (anti-chicken Alexa 568, 1:1000, A11041, Thermo Fisher Scientific; and anti-guinea pig Alexa 647, 1:1000, A-21450, Thermo Fisher Scientific) and washed again ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies were visualized using Fluor 594 Goat-α-mouse (1:1000, Invitrogen, A11032 and Alexa Fluor 488 Goat-α-Guinea pig (1:1000, Invitrogen, A11073). Free-floating sections were briefly washed in PBS followed by 1-2 h blocking in PBS containing 5% normal goat serum and 0.1% Triton X-100 at room temperature ...
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bioRxiv - Cell Biology 2020Quote: The frozen supernatants were used for IL-2 ELISAs according to the manufacturer’s protocol (KMC0021, Thermofisher).
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bioRxiv - Biochemistry 2022Quote: ... the plate was developed using 50 µL of 1-StepTM Turbo-TMB-ELISA Substrate Solution (ThermoFisher) per well and the plates were quenched with 50 µL of 2M H2SO4 to each well ...
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bioRxiv - Immunology 2021Quote: ... The plates were developed with 1-Step Ultra TMB-ELISA Substrate Solution (34028, Thermo Fisher Scientific) and the reaction was stopped by adding with 2 N sulfuric acid (BDH7500-1).
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bioRxiv - Cancer Biology 2019Quote: ... the concentration of Interferon-γ (IFNγ) in the media was measured by ELISA (Invitrogen, 88-7314). The T cells from 24-hour co-cultures were harvested ...
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bioRxiv - Immunology 2021Quote: ... The colorimetric reaction was terminated by the addition of 100 μL of ELISA Stop Solution (Invitrogen). The absorbance at 450 nm and 650 nm was read by spectrophotometry using a SpectraMax iD3 microplate reader (Molecular Devices).
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bioRxiv - Immunology 2019Quote: ... the concentration of IFN-γ in the cell culture supernatant was determined by ELISA (Invitrogen, #EHIFNG2). An IFN-γ standard was included in the ELISA and linear curve fitting using the GraphPad Prism 7.0 a software was used to derive the IFN-γ concentration in the samples from the absorbance at 450 nm (A450 ...
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bioRxiv - Cancer Biology 2020Quote: ... O.D values were determined at a wavelength of 590 nm using an ELISA reader (Thermo Scientific) The percentage survival was determined as the ratio of treated cells versus vehicle control after background subtraction ...
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bioRxiv - Microbiology 2021Quote: ... pH 9.6] was used to coat wells of an uncoated 96-well ELISA plate (Nunc Maxisorp) at 4°C overnight ...
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bioRxiv - Microbiology 2021Quote: ... The assay reaction was developed with the 1-step Ultra TMB-ELISA substrate (Thermo Fisher Scientific). The reaction was left to develop for 5 min and then stopped with 2M H2SO4 ...
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bioRxiv - Microbiology 2022Quote: ... Supplementary Table 3) and added (100 μL/well) to ELISA plates (Maxisorp Nunc-immuno plates; Thermo). Since Iwate/2019(H3N2 ...
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bioRxiv - Biochemistry 2021Quote: ... Background binding level in ELISA assays was measured using Human IgG Isotype Control (ThermoFisher #02-7102).
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bioRxiv - Immunology 2020Quote: ... plasma was serially diluted (4-fold dilutions) and added to ELISA plates (Maxisorp, Nunc, MA USA) pre-coated with 10μg/ml OVA (Sigma Grade V ...
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bioRxiv - Immunology 2019Quote: ... plasma was serially diluted (4-fold dilutions) and added to ELISA plates (Maxisorp, Nunc – MA, USA) pre-coated with 10µg/mL OVA (Sigma Grade V ...
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bioRxiv - Bioengineering 2021Quote: ... The signal was developed with 1-Step™ Ultra TMB-ELISA substrate solution (Thermo Fisher Scientific) and the reaction was stopped with 1% HCl ...
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bioRxiv - Neuroscience 2020Quote: ... Pierce HRP ECL substrate was used to detect luminescence in ELISA (Thermo Fisher Scientific, USA, #32209). Tris-buffered saline with Tween-20 (TBST ...
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bioRxiv - Immunology 2019Quote: ... Supernatants were harvested and assayed for IL-8 by ELISA (Thermo Fisher Scientific #88-8086-77). Alternatively ...
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bioRxiv - Immunology 2022Quote: ... Colorimetric detection was accomplished using 1-Step™ Ultra TMB-ELISA Substrate Solution (ThermoFisher Scientific: 34029) and color development was quenched with 1.0 N HCl ...
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bioRxiv - Immunology 2022Quote: ... 25 μL/well of 1-step Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific, cat. # PI34029) was added to the plates and incubated for 5 to 10 min at RT ...
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bioRxiv - Immunology 2022Quote: ... The color reaction was developed using the 1-StepTM Ultra TMB-ELISA substrate solution (Thermo Scientific) and plates were read at 450nm using the Labtech LT 4500 microplate reader ...
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bioRxiv - Microbiology 2022Quote: ... followed by subsequent addition of Turbo 3,3′,5,5′ tetramethylbenzidine (TMB)-ELISA substrate solution (Thermo Fisher Scientific) and 2M sulfuric acid stop solution ...
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bioRxiv - Microbiology 2022Quote: ... Supernatants were used to perform ELISA to quantify the levels of IL-1β (Thermo Fisher Scientific), IL-6 (Biolegend) ...
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bioRxiv - Neuroscience 2022Quote: ... the plates were incubated for 30 minutes with Pierce 1-Step Ultra TMB ELISA Substrate (ThermoFisher). Pre-warmed sulfuric acid stop solution (DY994 ...
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bioRxiv - Neuroscience 2022Quote: ... the plates were incubated for 30 minutes with Pierce 1-Step Ultra TMB ELISA Substrate (ThermoFisher). Pre warmed sulfuric acid stop solution (DY994 ...
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bioRxiv - Immunology 2022Quote: ... TNF concentrations in the supernatants were measured by ELISA (Fisher Scientific, Waltham, MA, Cat# 88-7346).
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bioRxiv - Cell Biology 2023Quote: ... 10μg purified FNBP4 was coated into the wells (maxisorp surface) of ELISA plates (#439454 Nunc Maxisorp) along with 1X PBS for the control experiment and allowed to incubate overnight at 4 °C ...