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Citations for Thermo Fisher :
1451 - 1500 of 10000+ citations for Pig Histone H3.3 H3F3A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2022Quote: ... The cDNA of CMAH was amplified from pig kidney issues and cloned into pcDNA3.1(+)-Myc vector (Invitrogen, Carlsbad, CA, USA) to yield the C-terminal Myc-tagged expression construct ...
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bioRxiv - Neuroscience 2019Quote: ... then incubated 2 hours in goat anti-guinea pig secondary antibody conjugated to Alexa Fluor 488 (1:500, Life Technologies, Carlsbad ...
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bioRxiv - Neuroscience 2021Quote: ... We used the following secondary antibodies conjugated with Alexa Fluor fluorochromes: 488 goat anti-guinea-pig IgG H+L (catalog #A11073, Invitrogen/ThermoFisher), 555 donkey anti-rabbit IgGs H+L (#A-31572) ...
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bioRxiv - Neuroscience 2021Quote: ... Alexa Fluor 633 goat anti-guinea pig (A21105, 1:500), Alexa Fluor goat anti-chicken (A21449, 1:750) (all Invitrogen), and Alexa Fluor 405 goat anti-chicken (Abcam ...
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bioRxiv - Cell Biology 2019Quote: ... 200 ul of a dilution of 1:200 of anti-guinea pig AlexaFluor 546 and anti-rabbit AlexaFluor 488 secondary antibodies (Invitrogen) in 20% FBS in PBS were added to the slides and incubated for two hours ...
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bioRxiv - Neuroscience 2020Quote: ... the tissue was incubated in secondary antibody (goat anti-rabbit IgG AlexaFluor 488, goat anti-guinea pig IgG AlexaFluor 647, 1:500, Invitrogen) and 1% NGS for 4 hours at room temperature ...
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bioRxiv - Genetics 2021Quote: ... Secondary antibodies used were goat anti-rabbit Alexa Flour-568 and goat anti-guinea pig Alexa Flour-488 (1:200) (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... slices were incubated with different Alexa-488 goat anti-mouse IgG1 or 568 goat anti-mouse IgG2b or 568 goat anti-rabbit or 647 goat anti-guinea pig secondary antibodies (1:1000; Invitrogen) accordingly for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... or guinea pig-anti-zebrafish-VE-cadherin 1:500 (Paatero, 2018) diluted in Pierce Immunostain Enhancer medium (Thermo Fisher Scientific). Alexa Fluor 405 goat anti-mouse immunoglobulin (IgG ...
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bioRxiv - Evolutionary Biology 2020Quote: ... membranes were washed 4 times with PBS-T and incubated with secondary antibodies: anti-guinea pig HRP (Invitrogen, #61-4620), and anti-rat HRP (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... followed by secondary antibodies (Ki67: Alexa 647 goat anti-rabbit [ab150155, Abcam, Cambridge, UK]; DCX: Alexa 555 goat anti-guinea pig [A21435, Invitrogen]) and nuclei were stained with DAPI ...
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bioRxiv - Immunology 2020Quote: ... IgG from CMAH/GGTA1 double knock out (DKO) pigs or rabbit IgG (WT) have been coated on Maxisorp™ (NUNC) ELISA plates at 10 μg/mL overnight at 4°C in carbonate buffer pH 9.5 ...
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bioRxiv - Neuroscience 2022Quote: ... followed by secondary antibody-staining with Alexa 647-tagged goat anti-guinea pig antibody (catalog no.A21450, Thermo Fisher Scientific, USA), after which the sections were rinsed ...
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bioRxiv - Neuroscience 2022Quote: ... for ASO: anti-rabbit Alexa Fluor Plus 647, A32795 ThermoFisher Scientific; for NeuN: anti-guinea pig Alexa Fluor 555, A21435 Invitrogen or anti-chicken Alexa Fluor 594 ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were washed and incubated with Alexa Fluor 594 donkey anti-rabbit antibody and Alexa Fluor 488 anti-guinea pig (ThermoFisher) at 1:2000 in 0.3% Triton in 0.1 M PB ...
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bioRxiv - Molecular Biology 2023Quote: ... the supernatant of human coronary artery smooth muscle cells overexpressing ADAMTS7 or a mock plasmid was used with fluorescein conjugates of gelatine (DQ Gelatine from Pig Skin, ThermoFisher). Supernatants were plated on a flat black 96-well plate (Nunclon ...
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bioRxiv - Biochemistry 2023Quote: ... membranes were incubated at RT for 1 h with 1:1000 dilution of horseradish-peroxidase conjugated rabbit anti-guinea pig IgG secondary antibody (Invitrogen), or with 1:2500 dilution of horseradish-peroxidase conjugated donkey anti-mouse IgG secondary antibody (Jackson ImmunoResearch ...
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bioRxiv - Developmental Biology 2023Quote: ... goat anti-mouse or anti-rabbit or anti-guinea pig IgG coupled to AlexaFluor-488 or AlexaFluor-546 or AlexaFluor-647 (1:500, Invitrogen), anti-chicken IgG coupled to AlexaFluor-488 (1:1500 ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated with secondary antibodies (goat anti-mouse Alexa488, goat anti-rabbit Alexa 594, goat anti-guinea pig Alexa 647, 1:1,000, Thermo Fisher) for 4 h at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... samples were incubated for 1h with a goat anti-guinea pig secondary antibody (Alexa Fluor 488 conjugate; 1:1000; Invitrogen) and wheat germ agglutinin to label the nuclear envelope (Alexa Fluor 647 conjugate ...
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bioRxiv - Physiology 2023Quote: ... This was followed by a secondary antibody incubation with either goat anti-guinea pig (Alexa fluor 488, A11073, Life Technologies), donkey anti-goat (Alexa fluor 633 ...
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bioRxiv - Cancer Biology 2024Quote: ... and goat-anti-guinea pig-IgG-Alexa Fluor-647 (1:400, A21450, all Thermo Fisher Scientific, Waltham, MA, United States). The actin cytoskeleton was stained using phalliodin-PF647 for 30 min (1:50 ...
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bioRxiv - Molecular Biology 2024Quote: ... Then the slides were washed twice with 1X PBS for 5 minutes and incubated with secondary antibodies: Alexa-Fluor 488 goat anti-guinea pig (A11073; Invitrogen), Alexa-Fluor 568 goat anti-mouse (A11031 ...
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bioRxiv - Neuroscience 2024Quote: ... for 20 min at RT and incubated with 3% NGS PBS containing secondary antibodies (1:600, Alexa-Fluor 594 anti-mouse IgG, Invitrogen and Alexa-Fluor 488 anti-guinea pig IgG, Invitrogen) for 90 min at RT ...
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bioRxiv - Neuroscience 2024Quote: ... goat anti-mouse or anti-guinea pig or anti-rabbit Alexa Fluor 488 (1:500, Life Technologies A11001/A11073/A11008). Larvae were mounted in Vectashield (Vector Labs) ...
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bioRxiv - Neuroscience 2024Quote: ... slices were washed three times in PBS and the incubated in AlexaFluor secondary antibodies goat anti-guinea pig 647 and goat anti-rabbit 594 (Invitrogen) for 1 hour at room temperature.
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bioRxiv - Biophysics 2022Quote: Fluorescent labeling was performed by targeting histone amine groups of pre-assembled nucleosomes using Alexa Fluor 647 succinimidyl ester (ThermoFisher), following the previously reported strategy20 ...
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bioRxiv - Biophysics 2022Quote: ... the 100 μL mixture of DNA and core histones was put into a Slide-A-Lyzer device of 7000 MWCO (ThermoFisher) and dialyzed overnight against low salt buffer (25 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... polyclonal anti-human MPO (IgG, sheep, 1:50) (AA16-718, Antibody online), monoclonal anti-human Histone H1 (IgG2a, mouse, 1:100) (MA5-13750, Invitrogen), or monoclonal anti-human NPM1 (IgG1 ...
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bioRxiv - Bioengineering 2023Quote: ... cells from the MDA-MB-231 cell line (ATCC) with nuclear GFP (histone transfection) were thawed and cultured at 5% CO2 and 37 °C in DMEM (Gibco) at pH 7.2 supplemented with 10% fetal bovine serum (Wisent Bioproducts) ...
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bioRxiv - Cell Biology 2020Quote: ... ELISA absorbance was measured at 450nm and normalized to total DNA content (SYBRGreen; Invitrogen) using an absorbance/fluorescence microplate reader (BioTek) ...
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bioRxiv - Immunology 2021Quote: ... Heat-inactivated HSV-1 McKrae strain was used for coating ELISA plates (Nunc Immunosorbent). The affinity of binding of antigen-specific antibodies to the HSV-1 McKrae strain was measured by ELISA plates coated overnight at 4°C with (104 pfu/ well ...
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bioRxiv - Molecular Biology 2020Quote: ... Plates were coated with 5 μg/ml TciALDO onto ELISA plates (Maxisorp, Thermofisher Scientific) overnight at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... IL-6 ELISA assay was performed on collected media (Affymetrix eBioscience, #88-7066-22).
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bioRxiv - Physiology 2020Quote: ... The ELISA was read using a filter-based accuSkan FC micro photometer (Fisher Scientific). The limits of sensitivity were > 0.188 ng/ml and > 0.375 ng/ml for MUC5AC and MUC5B ...
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bioRxiv - Immunology 2021Quote: ... 50 μl/well of 1-Step™ Ultra TMB-ELISA Substrate Solution (ThermoFisher, 34028) was applied and the enzyme-substrate reaction was stopped after six minutes using 50 μl/well sulphuric acid (1 M H2SO4) ...
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bioRxiv - Microbiology 2020Quote: A 96-well enzyme-linked immunosorbent assay (ELISA) plate (Nunc MaxiSorp, Thermo Fisher Scientific) was first coated overnight with 100 ng per well of purified recombinant protein in PBS buffer ...
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bioRxiv - Microbiology 2020Quote: A 96-well enzyme-linked immunosorbent assay (ELISA) plate (Nunc MaxiSorp, Thermo Fisher Scientific) was first coated overnight with 100 ng per well of purified recombinant protein in PBS buffer ...
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bioRxiv - Microbiology 2021Quote: ... Flat bottom 96-well ELISA plates (Nunc MaxiSorp Plates, Thermo Fisher Scientific, Planegg, Germany) were coated with 50 ng/well recombinant 2019-nCoV (COVID-19 ...
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bioRxiv - Immunology 2022Quote: ... the reaction was terminated with equal volume of ELISA Stop Solution (Thermo Fisher Scientific). Absorbance at 450 nm was read using the BioTek plate reader ...
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bioRxiv - Immunology 2022Quote: ... the reaction was terminated with equal volume of ELISA Stop Solution (Thermo Fisher Scientific). Absorbance at 450 nm was read using the BioTek plate reader ...
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bioRxiv - Immunology 2022Quote: ... a 96-well flat-bottom NUNC PolySorp ELISA plate (Thermo Fisher Scientific, cat: 456529) was sensitised with 100 µL of 95 µM Streptavidin (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... was coated on Nunc Maxisorp ELISA plates (Thermo Fisher Scientific, Cat# 44-2404-21) at 2.5 µg/mL final concentration in Sodium Bicarbonate buffer (Sigma-Aldrich ...
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bioRxiv - Biophysics 2020Quote: 4ug/mL ACE2 in 1xPBS pH 7.4 was coated onto ELISA strips (Thermo Fisher) for 1 hr and then blocked with 3% BSA solution (1x PBS ...
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bioRxiv - Immunology 2020Quote: ... Cytokine concentrations were measured by ELISA or Luminex Multiplex Assay (Invitrogen Mouse 20-plex). To determine host susceptibility to endotoxemia ...
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bioRxiv - Biochemistry 2021Quote: ... 150 μL of TMB (1-Strep Ultra ™B-ELISA, ThermoFisher Scientific, Illkirch, France) distributed in each well ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were incubated with 200 μL of TMB-ELISA (Thermo Scientific, Cat. no: 34028) substrate till the appearance of a light blue color and reaction was stopped by transferring 100 μL of this solution to a different 96-well plate containing 100 μL of 1 M H2SO4 ...
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bioRxiv - Bioengineering 2020Quote: ... and 100 μl dispensed in triplicate into wells of 96-well ELISA plates (Nunc Maxisorp ...
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bioRxiv - Molecular Biology 2022Quote: ... either 100uL or 20uL supernatant was collected for aldosterone or cortisol competitive ELISA (Invitrogen), respectively ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Bound secondary antibody was detected using Ultra TMB One-Step ELISA substrate (Thermo Fisher). After quenching the reaction with sulfuric acid ...