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Citations for Thermo Fisher :
1701 - 1750 of 10000+ citations for Pig Histone H3.3 H3F3A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... 0.5ug/mL of BA.1 RBD protein were coated on 96-well ELISA plates (Nunc Maxisorp) and left overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Absorbance was measured at a wavelength of 450 nm using an ELISA microplate reader (Thermo Fisher). Assays were repeated at least three times.
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bioRxiv - Immunology 2023Quote: ... supernatant was collected and IFN-ɑ measured by ELISA according to manufacturer’s instructions (Invitrogen; catalog # BMS6027)
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bioRxiv - Microbiology 2023Quote: ... and the absorbance was measured at 450 nm in an ELISA plate reader (Varioskan, Thermo Scientific).
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bioRxiv - Immunology 2024Quote: ... the plates were washed with TBS-T and SuperSignal™ ELISA Femto Maximum Sensitivity Substrate (ThermoFisher) was added following manufacturer’s instructions ...
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bioRxiv - Pathology 2024Quote: ... The total amount of TGFβ1 was determined on technical duplicates by ELISA (88-8350-22, Invitrogen), after heat denaturation followed by acidic treatment and neutralization according to the manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Plates were washed and then developed by adding Slow Step ELISA TMB substrate (Thermofisher, Cat: 34024) to each well ...
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bioRxiv - Biochemistry 2024Quote: ... Signal was developed by treating cells with 200 μl TMB-ELISA (Thermo Scientific, Cat no. 34028) until the light blue colour appeared ...
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bioRxiv - Biochemistry 2023Quote: ... 100 μl horseradish peroxidase substrate (1-Step™ Ultra TMB-ELISA Substrate Solution) (Thermo Scientific™) was added into wells followed by 100 ul 1M H2SO4 ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatants were harvested for IL-1β measurement using a specific ELISA (Thermo Fisher, 88-7621-22). Apoptotic cells were generated by retroviral transduction or MTX treatment during 20-24 h when cell death approaches its maximum ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were incubated sequentially with primary antibodies that detect either FLAG or phospho-Histone H2A.X (Ser139) and secondary antibodies (Alexa 488, Molecular Probes, Eugene, OR, US). Coverslips were mounted and cells were counterstained on the glass slides using ProLong Gold with DAPI (4’ ...
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bioRxiv - Cell Biology 2020Quote: ... The secondary antibodies used were Alexa 488 goat anti-rabbit IgG and Alexa 568 goat anti-guinea pig IgG (Thermo Fisher Diagnostics, Tokyo, Japan). The lack of cross-reactivity of the antibodies was also confirmed (data not shown) ...
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bioRxiv - Genomics 2019Quote: ... followed by goat anti-guinea pig Alexa-Fluor-488 and goat anti-mouse Alexa-Fluor-555 (diluted 1:400, Thermofisher A11073 and A21422, respectively) secondary antibodies ...
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated for 2 hours in anti-mouse antibodies labelled with Alexa Fluor 488 and anti-guinea pig antibodies labelled with Alexa Fluor 568 (1:150) (Life Technologies, Carlsbad, CA, USA). Imaging and data processing were the same for Fly retina immunostaining.
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bioRxiv - Cell Biology 2021Quote: ... 50 μL of secondary antibody solution for the final concentration of 1:1000 (Alexa Fluor 488 goat anti-guinea pig IgG, Thermo Scientific A-11073; Alexa Fluor 647 goat anti-rabbit IgG, Thermo Scientific A-21244) in 5% normal goat serum in PBS was added and incubated for 1.5 hr ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated with one or more of the following primary antibodies (rabbit anti-TH, 1:2000, AB152, EMD Millipore; guinea pig anti-VGLUT2, AB2251, EMD Millipore; chicken anti-GFP, A10262, Invitrogen, Carlsbad, CA) in blocking buffer (overnight ...
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bioRxiv - Neuroscience 2022Quote: ... A subset of sections from brains with FLEX-SomArchon expression were stained with a guinea pig anti-PV antibody (GP72, Swant, 1:1000) following by Alexa Fluor 568 goat anti-guinea pig secondary antibody (No: A-11075, Thermo Fisher Scientific, 1:500). All antibodies were diluted in 2% normal goat serum and 0.5% Triton-X (No ...
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bioRxiv - Neuroscience 2022Quote: ... Coverslips then were washed 3×10 min with 1% NDS in 0.1 M PBS at room temperature followed by the incubation with goat anti-guinea pig Alexa Fluor 488 (4 µg/ml, Invitrogen, catalog #A-11073, RRID:AB_2534117) and donkey anti-rabbit Alexa Fluor 594 (4 µg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated for 2 h at room temperature in the appropriate combinations of Alexa 488-594-647-conjugated anti-mouse/rabbit/guinea pig or rat IgG secondary antibodies (1:500; Molecular Probes, Eugene, OR, USA). Sections were also stained with a nuclear stain DAPI (4,6-diamidino-2-phenylindole ...
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bioRxiv - Physiology 2023Quote: EAT (occluded and non-occluded tissue pooled) from exercise-trained (n=4) and sedentary (n=3) female pigs had total RNA extracted with TRIzol® (Thermofisher Scientific, Waltham, MA). Total RNA was quantified (Nanodrop 3300 ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated with secondary antibodies (1:500, goat anti-guinea pig Alexa488; goat anti-rabbit Alexa597; goat anti-mouse Alexa647 [Life Technologies, Eugene, OR, USA]) diluted in the same solution as primary antibody for 2 h in the dark at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed 3× with PBST before 1 hour incubation at room temperature with secondary antibodies (α-guinea pig Alexa-488, α-rabbit Alexa 568, α-chicken 647 along with DAPI) from Molecular probes and Jackson Laboratories) ...
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bioRxiv - Neuroscience 2024Quote: ... Olean, NY, Cat#DI-1094 and 1:200 goat-anti guinea pig 488, Abcam, Cambridge, UK, Cat#150185 or Invitrogen, Waltham, MA, Cat#A11073) were applied at room temperature for 30 minutes ...
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bioRxiv - Biochemistry 2021Quote: Separation of digested histone peptides was performed by using nanoliter flow liquid chromatography using an EASY-nLC nanoHPLC (Thermo Scientific, San Jose, CA, USA) equipped with an analytical weak cation exchange–hydrophilic interaction liquid chromatographic resin (PolycatA ...
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bioRxiv - Neuroscience 2022Quote: Levels of specific histone modifications at the BDNF P2 promoter were determined by quantitative real-time PCR (StepOne Plus; Applied Biosystems, Foster City, CA). The following primers were used to amplify portions of the BDNF P2 promoter ...
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bioRxiv - Biophysics 2022Quote: We cultured MDA-MB-231 breast cancer cells stably transduced with histone-2B mCherry (gift from Timo Betz, WWU Münster) in L15 medium with 2 mM Glutamax (Thermo Fisher Scientific, Waltham, MA USA) plus 10% fetal bovine serum (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... Protein secretion levels in culture supernatant were quantified via ELISA for human IgG (Invitrogen, 50-112-8849) and human BAFF (R&D Systems ...
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bioRxiv - Immunology 2021Quote: ... then TMB substrate (1-Step™ Ultra TMB-ELISA Substrate Solution, Thermo Fisher Scientific, Waltham, MA, USA) was added and developed for approximately 1 minute before stopping the reaction with 1 N HCl ...
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bioRxiv - Immunology 2020Quote: ... perfringens native ECP or native CWP were coated on ELISA plates (Nunc MaxisorpTM, ThermoFisher Scientific, Waltham, MA) at 10 μg/mL (IgA ...
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bioRxiv - Molecular Biology 2020Quote: ... Solid phase ELISA was performed by coating recombinant NEDP1 (20μg/well) on Maxisorb 96 well plates (Nunc) at 4°C overnight ...
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bioRxiv - Neuroscience 2021Quote: ... ISF Aβ42 in the 14 samples for each mouse was measured by ELISA (Invitrogen, KHB3441, 1:4). To determine Aβ42 half-life (Cirrito et al. ...
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bioRxiv - Immunology 2022Quote: Cell culture supernatants from BMDCs were analyzed by ELISA for IL-12p70 (R&D) and IFNβ (Invitrogen). Plates were read using a Tecan Sunrise microplate reader at 450 nm ...
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bioRxiv - Immunology 2021Quote: ... the assay was developed by addition of 1-Step Ultra TMB-ELISA solution (Thermo Scientific Cat# 34028) and reaction was stopped with 2 N sulfuric acid (Fisher Scientific) ...
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bioRxiv - Bioengineering 2021Quote: ... 2X 90μl of the CM was placed to microwells of 96 well ELISA plates (NUNC MaxiSorp™) as duplicates ...
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bioRxiv - Biochemistry 2020Quote: ... Free LC and IgG concentrations were determined by ELISA in 96-well plates (Immulon 4HBX, Thermo Fisher). Wells were coated overnight at 37 °C with sheep polyclonal free λ LC antibody (Bethyl Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... ELISA: The assay was routinely performed in high binding 96-wells plates (Immulon 4 HBX, Thermo Fisher). Washings throughout the assay were ...
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bioRxiv - Bioengineering 2021Quote: ... A colorimetric detection was performed using a 1-Step Ultra TMB ELISA substrate solution (Thermo Fisher Scientific) and OD450 was measured with a SpectraMax Plus 384 microplate reader (Molecular Devices) ...
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bioRxiv - Bioengineering 2021Quote: ... A colorimetric detection was performed using a 1-Step Ultra TMB ELISA substrate solution (Thermo Fisher Scientific) and OD450 was measured with a SpectraMax Plus 384 microplate reader (Molecular Devices).
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bioRxiv - Microbiology 2021Quote: ... Protein levels of IL-18 from intestinal biopsies were measured by ELISA (BMS618-3, ThermoFisher, Waltham, MA). Assays were performed according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: An ELISA for Pygo2 was designed by coating a streptavidin-coated 96-well plate (Thermo Fisher, 15125) with biotinylated 21-mer H3K4me2 peptide (Active Motif ...
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bioRxiv - Microbiology 2022Quote: Recombinant human CD81 protein (R &D Systems) was coated (4µg/ml) on ELISA plate (Thermo Fisher Scientific) and incubated overnight at 40C ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, ThermoFisher, for RBD-ELISA ...
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bioRxiv - Microbiology 2020Quote: ... was performed prior to incubation with 1-Step™ Ultra TMB-ELISA Substrate Solution (ThermoFisher Scientific, CA) for color development ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, ThermoFisher, for RBD-ELISA,and BD OptEIA Substrate Solution ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were validated using PCR and western blot and screened for steroidogenic induction using aldosterone ELISAs (Invitrogen).
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bioRxiv - Immunology 2022Quote: ... Horseradish peroxidase activity was detected with 50 µL 1-Step Ultra TMB ELISA substrate solution (Thermo Fisher). Then the reaction was stopped with 50 µL 10% H2SO4 and the absorbance was measured at 450 nm ...
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bioRxiv - Immunology 2020Quote: ISG15 ELISA was performed by coating plate with diluted rabbit anti mouse ISG15 (PA5-17461; Thermo Scientific) 1:1000 in PBS overnight ...
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bioRxiv - Microbiology 2020Quote: ... The lysates were coated onto the ELISA plate (Nunc-Immuno™ plate; Thermo Fisher Scientific, Roskilde, Denmark). The antigen-coated wells were then blocked with 20% Blocking One (Nacalai Tesque ...
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bioRxiv - Microbiology 2021Quote: ... Activation of HRP conjugated antibody was detected by room temperature 1-step Ultra TMB ELISA (Thermo Scientific) for 10 min with rocking in the dark ...
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bioRxiv - Biochemistry 2020Quote: ... The expression of the recombinant protein was measured by ELISA using anti‐His tag monoclonal antibody (Invitrogen) to capture and biotinylated anti‐ANG1 antibody for detection (R&D System) ...