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Citations for Thermo Fisher :
1651 - 1700 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: PBMC derived CD4+ T cells were isolated from HIV-infected MACS participants using EasySep™Human CD4+ T Cell Isolation Kit and activated overnight with Human T-Activator CD3/CD28 Dynabeads® (Life Technologies). The next day Dynabeads were separated from the CD4+ T cells by manual dissociation followed by magnet isolation ...
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bioRxiv - Immunology 2021Quote: ... CD4+ and CD8+ T cells were isolated by negative selection with the Dynabeads Untouched Human CD4 T Cells Kit and the Dynabeads Untouched Human CD8 T Cells Kit (Invitrogen, Thermo Fisher Scientific) respectively ...
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bioRxiv - Cell Biology 2022Quote: Human cerebral organoids are generated from human induced pluripotent stem cells (iPSCs) which were purchased from GibcoTM (Gibco by Thermofisher, Waltham, Massachusetts, USA). iPSCs were plated in a dish pre-coated with Matrigel® hESC-Qualified Matrix (Corning ...
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bioRxiv - Cell Biology 2023Quote: We profiled the expression of transcripts in human aortic vascular smooth muscle cells using the Clariom D human DNA microarray (Thermo Fisher Scientific Inc.). This platform includes information from probe sets representing known and predicted exons and introns on both strands of the genome that have been mapped to more than genes ...
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bioRxiv - Immunology 2023Quote: Customized cytokine 25-Plex Human ProcartaPlex Assay (customized from Cytokine 25-Plex Human ProcartaPlex Panel 1B with the catalog # EPX250-12166-901, Thermo Fisher Scientific, USA) was used to measure multiple cytokines in the supernatants of stimulated human PBMCs.
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bioRxiv - Evolutionary Biology 2020Quote: ... Three to six transformed colonies were cultured in 5 ml of LB medium and plasmids were subsequently purified with a GeneJET Plasmid Miniprep kit (Thermo Fisher Scientific). Purified plasmids were sequenced by Eurofins Genomics (Fig ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Large quantities of purified plasmids containing AsegIRs and AsegIR8a with verified sequences were obtained using the PureLinkTM HiPure Plasmid Filter Midiprep Kit (Thermo Fisher Scientific). cRNAs of AsegIRs and AsegIR8a were synthesized from NotI (Promega ...
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bioRxiv - Microbiology 2022Quote: ... Expi293F cells at 2 × 106/ml were transfected with a mixture of 1 ng of library plasmid with 1 μg of pMSCV as a junk plasmid per ml using ExpiFectamine (Thermo Fisher Scientific). Twenty-four hours after transfection ...
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bioRxiv - Cell Biology 2019Quote: ... 5 µg of lentiviral plasmid packaging mix (24) and 5 µg of pooled sgRNA library plasmid was transfected using lipofectamine 2000 (ThermoFisher Cat# 11668019) and incubated for 2 days ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were co-transfected with 1 μg of the corresponding plasmids and 10 ng of a Cre recombinase plasmid using Lipofectamine 2000 (Thermo Fisher Scientific). Stable cell lines were selected after 2 days of growth by addition of 1 μg ml−1 puromycin ...
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bioRxiv - Immunology 2019Quote: ... The sgRNA was cloned pCas-Puro-U6 plasmid and the pCas-Puro-U6 plasmid Linear was obtained using the BbsI restriction enzyme (Thermo Fisher Scientific). The plasmids containing sgRNA were transfected into IPEC-J2 cells with GeneTran III (Biomiga ...
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bioRxiv - Microbiology 2019Quote: ... 293T cells in 6-well plates were transfected with 1.0 μg pMJ920 plasmids and 1.0 μg gRNA expression plasmids by Lipofectamine® 2000 Transfection Reagent (Invitrogen, Cat.11668-027) using the recommended protocols ...
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bioRxiv - Genomics 2020Quote: ... Correct assembly of the sgRNA into the backbone was confirmed by SANGER-Sequencing (Microsynth) and plasmids were isolated using a GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... 2.5 μg FLAG-hRpn13-expressing plasmid or 5 μg FLAG-hRpn131-279-expressing plasmid by using lipofectamine 3000 (Thermo Fisher Scientific, L3000015) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... these plasmids were transiently transfected into HEK293T cells with packaging plasmid psPAX2 and envelope plasmid pMD2.G using Lipofectamine 3000 transfection reagent (Thermo Fisher, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... sgRNA expressing plasmids were produced by cloning sgRNA oligonucleotides with the appropriate adaptor sequences (see Table S2) into plasmid pMCB306 after digesting the plasmid with restriction enzymes BstXI and Bpu1120I (Cat# FD1024, FD0094, Thermo Fisher Scientific). pMCB306 was a gift from Michael Bassik (Addgene plasmid #89360 ...
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bioRxiv - Plant Biology 2021Quote: ... Positive colonies were grown in LB overnight and then used for subsequent plasmid isolation (GeneJET Plasmid Miniprep Kit; Thermo Fisher Scientific, USA). About 150 pUC18R6KT-mini-Tn7T-Km recombinant plasmids were stored at −4°C ...
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bioRxiv - Microbiology 2021Quote: ... The resulting sub-cloning plasmids and the conjugative plasmid pVZ321 were cleaved with restriction enzymes XhoI and SalI (Thermo Scientific™, Germany), generating compatible restriction sites ...
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bioRxiv - Molecular Biology 2021Quote: ... Large quantities of purified plasmids containing ItypORs and ItypOrco with correct sequences were obtained using the PureLinkTM HiPure Plasmid Filter Midiprep Kit (Thermo Fisher Scientific). cRNAs of ItypORs and ItypOrco were synthesized from NotI (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... For lentivirus particle production these plasmids were recombined with pCDH-EF1a-Gateway-IRES-BLAST plasmids as described in [51] using LR Clonase II (Invitrogen/Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... 100 million HEK293T cells were seeded into a 15 cm2 plate with complete DMEM and transfected with 5 μg of lentiviral plasmid packaging mix and 5 μg of pooled sgRNA library plasmid using lipofectamine 2000 (ThermoFisher Cat# 11668019) and incubated for 2 days.
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bioRxiv - Molecular Biology 2022Quote: ... Cells were seeded at 1×105 cells per well in a 24 well multiplate and transfected with 800 ng plasmid DNA per well (400 ng of SaCas9 expression plasmid and 200 ng of each sgRNA plasmid) using Lipofectamine 2000 (Thermo Fisher Scientific) as according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... incubated overnight at 37°C in shaker and the plasmid DNA was extracted using GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific, Lithuania). As the final means of verification sequence of inserted fragment was verified through Sanger Sequencing employing M13-Fw and M13-Rs primers ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µg of each plasmid DNA (two plasmids expressing Cas9 and gRNAs) were mixed in 100 µl of reduced-serum medium OPTI-MEM (ThermoFisher Scientific, 31985062) then mixed with 15 µl of 1 mg/ml PEI ...
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bioRxiv - Plant Biology 2021Quote: ... Plasmid DNA of the final constructs was isolated with a PureLink™ HiPure Plasmid Filter Midiprep Kit (Invitrogen™ /Thermo Fisher Scientific) according to the manufacturer’s manual ...
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bioRxiv - Plant Biology 2021Quote: ... Plasmid DNA of the final constructs was isolated with a PureLink™ HiPure Plasmid Filter Midiprep Kit (Invitrogen™ /Thermo Fisher Scientific) according to the manufacturer’s manual ...
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bioRxiv - Immunology 2020Quote: ... PSG3 5 plasmid was co-transfected into 293T cells along with various HIV envelope plasmids 6 using Lipofectamine 2000 transfection reagent (ThermoFisher Scientific, #11668019) to produce single-round of infection competent pseudo-viruses representing multiple clades of HIV ...
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bioRxiv - Cancer Biology 2022Quote: Lentivirus was produced by transfection of HEK293T packaging cells with three packaging plasmids (pMDL, pRSV-REV, pCMV-VSVG) [30] and the lentiviral reporter plasmid using Lipofectamine 3000 (Invitrogen, Waltham, MA) according to the manufacturer’s recommended protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... MCF10A cells were co-transfected with 1000 ng of pHrD-IRES-Luc plasmid 16 and 0.1 ng of a CMV-Renilla constitutive internal control plasmid using Lipofectamine 3000 (Thermo Fisher Scientific, L3000015) per manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: We cotransfected 10-12 replicates of HCT-116 cells with 5 µg of PB-SRT-Puro plasmid and 5 µg PBase plasmid via Neon electroporation (Thermo Fisher #MPK10025). Each replicate contained 2×106 cells ...
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bioRxiv - Molecular Biology 2020Quote: Both the constructed all-in-one CRISPR plasmids and dual colored KI plasmids were co-transfected into the mESCs using lipofectamine 3000 (Thermo Fisher Scientific). Dissociated mESCs were plated on gelatin-coated 24-well plates with 500 μl of (t)2iL+Y-27632 medium ((t)2iL+Y) ...
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bioRxiv - Microbiology 2020Quote: ... [46] cells were transfected with BZLF1 expression plasmid together with the gB expression plasmid using the Neon Transfection System (Thermo Fisher Scientific) to induce lytic replication ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Three to six transformed colonies were cultured in 5 ml of LB medium and plasmids were subsequently purified with a GeneJET Plasmid Miniprep kit (Thermo Fisher Scientific). Purified plasmids were sequenced by Eurofins Genomics.
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bioRxiv - Molecular Biology 2019Quote: ... MCF7 cells were transfected with equimolar amounts of luciferase reporter plasmids and pRL-TK transfection control plasmid with Lipofectamine 3000 (Thermo Fisher Scientific).Luciferase activity was measured 24 h post-transfection by the Dual-Glo Luciferase Assay System (Promega) ...
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bioRxiv - Cell Biology 2021Quote: ... MCF10A ARPIN KO cells were transfected with the Cas9- and gRNA-containing pX330 plasmid and the donor plasmids described above using Lipofectamine 3000 (Thermo Fisher Scientific). Cells were selected with 0.5 µg/ml of puromycin (InvivoGen) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Expi293F cells were transfected at a density of 2 × 106 /ml with 400 ng (pCEP4-myc-ACE2 plasmids) or 500 ng (pCEP4-myc-S plasmids) per ml of culture using ExpiFectamine 293 (Thermo Fisher Scientific) according to the manufacturer’s directions ...
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bioRxiv - Biochemistry 2021Quote: ... A positive clone was selected from Petri-plates and then plasmid from positive clone was extracted using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific). The integrity of cloned gene in the pET28a in terms of orientation and sequence was checked at this stage ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Cells were co-transfected with 800 ng of plasmid of interest and 150 ng plasmid containing EF1a upstream of GFP for normalization with Lipofectamine Stem transfection reagent (Thermo Fisher scientific) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... HEK293Ts cells were transfected with 2nd generation lentiviral packaging plasmids and transfer plasmid using Lipofectamine 3000 transfection reagent (Fisher Scientific cat L3000075). In detail ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were seeded in 6-well plates 24 hours before transfection with ADAR2 expressing plasmids using 400 ng plasmid DNA and Lipofectamine 3000 reagent (Thermo Fisher Scientific) according to manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... The recombinant T/F LTR U3R-pGL3 reporter plasmid was then purified from a randomly picked colony using GeneJet Plasmid Mini Prep Kit (Invitrogen, Carlsbad, CA) as per the manufacturers’ instructions ...
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bioRxiv - Plant Biology 2022Quote: ... the entry clones with homologous arms and the pDONR-R4R3-loxP-Hygro-loxP plasmid were recombined into the pGEM-gate plasmid in a three-fragment multisite Gateway recombination (Invitrogen, CA, USA), using an LR II clonase plus reaction ...
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bioRxiv - Molecular Biology 2022Quote: ... CRE-nls-GFP ORF was amplified from addgene plasmid #49056 and inserted into the adenoviral expression vector pAd/CMV/V5-DESTTM Gateway Vector plasmid (Thermo Fisher Scientific). After production and amplification of the adenovirus ...
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bioRxiv - Bioengineering 2024Quote: ... For endogenous RNA trans-splicing experiments 400 ng of dCas13 expression plasmid was mixed with 400 ng of rcRNA expression plasmid 10ul R Buffer (Thermo Fisher Scientific). For experiments where only dCas13 expression plasmid or rcRNA plasmid was transfected ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting Gateway entry plasmids were further recombined with the pCDH-EF1a-Gateway-IRES-BLAST destination plasmid using the Gateway LR recombination kit (Invitrogen, cat. #11791020). All cloning vectors were kindly supplied by Dr ...
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bioRxiv - Microbiology 2024Quote: ... The pEcgRNA-guide plasmids from an overnight culture of Stellar cells expressing the plasmids grown in LB medium with spectinomycin (50 µg/ml) were extracted using GeneJET Plasmid Miniprep Kit (Thermo Scientific™). The pEcgRNA-guide plasmids were individually cloned into LT2c ΔStyLTI competent cells harboring the pEcCas plasmid and plated on both spectinomycin for selection of pEcgRNA (50 µg/ml ...
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bioRxiv - Biochemistry 2024Quote: ... 2 million cells were plated in 10-cm tissue culture plates and co-transfected the next day with 475 ng of plasmid encoding bxb1 recombinase and 7125 ng of the plasmid library using Lipofectamine 3000 (Invitrogen, Carlsbad, CA). Cells were grown at 33 °C for 4 days after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... pLVX-DsRed-Spartin plasmid together with psPAX2 and VSV-G plasmid were transfected into HEK293 cells using Lipofectamine 3000 (L3000015; Thermo Fisher Scientific) for generating lentivirus ...
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bioRxiv - Microbiology 2023Quote: ... Positive transformants were inoculated in 5 mL liquid LB medium (omitting the Bacto agar) and plasmids were isolated using a GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific). Correct plasmid structure was confirmed using restriction analysis using the FastDigest KpnI enzyme (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Expi293F cells at 2 × 106 cells per ml were transfected with a mixture of 1 ng of library plasmid with 1 μg of pMSCV empty plasmid per ml using ExpiFectamine (Thermo Fisher Scientific). Twenty-four hours after transfection ...