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Citations for Thermo Fisher :
1551 - 1600 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Human IL-6 ELISA kit was from Life Technologies. FAN1KO HD patient fibroblasts were generated and received from Synthego ...
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bioRxiv - Genomics 2023Quote: ... and Human Brain Reference RNA (QS0611, Thermo Fisher Scientific) were used ...
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bioRxiv - Genomics 2023Quote: ... The Human Gene 2.1 ST Array platform from Affymetrix produced the data ...
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bioRxiv - Immunology 2023Quote: 6.5 million human embryonic kidney (HEK) 293 cells (Gibco) were seeded the day before transfection in 150mm dishes in DMEM (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... or human TIA-1 (Assay ID 139893-Thermofisher scientific) or GFP targeting siRNA as a negative control (cat no ...
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bioRxiv - Cancer Biology 2023Quote: Microarray expression data (Affymetrix Human Gene 2.0 ST arrays) from Nicolle et al ...
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bioRxiv - Biophysics 2023Quote: ... media-washed human activator CD3/CD28 Dynabeads (Gibco, USA) were added at a 1:1 cell-to-bead ratio ...
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bioRxiv - Immunology 2023Quote: ... PE-Cy7 anti-human C27 (clone O323, ThermoFisher Scientific), AlexaFluor 700 anti-human CD38 (clone LS198-4-3 ...
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Metabolic specialization drives reduced pathogenicity in Pseudomonas aeruginosa CF clinical isolatesbioRxiv - Microbiology 2023Quote: ... previously coated with human type I collagen (Gibco, A1048301). ALI was established once cells reached full confluency by removing media from the apical chamber and replacing media in the basolateral chamber with Pneumacult-ALI maintenance medium (STEMCELL Technologies ...
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Tight junction membrane proteins regulate the mechanical resistance of the apical junctional complexbioRxiv - Cell Biology 2023Quote: ... rabbit polyclonal anti-human JAM-A (Invitrogen; #PA5-120157); rabbit polyclonal anti-CAR (kindly provided by Jeffrey M ...
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bioRxiv - Molecular Biology 2023Quote: Human HEK293T cells (ATCC) were maintained in DMEM (Gibco) supplemented with 10% heat inactivated FBS (Gibco ...
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bioRxiv - Immunology 2023Quote: ... 50 ng/mL human IL-21 (Thermo Fisher Scientific), 50 ng/mL human IL-4 (Miltenyi) ...
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bioRxiv - Systems Biology 2024Quote: ... ANXA1 (rabbit anti-human, 1:1000, ThermoFisher Scientific, UK), phosphotyrosine-21 ANXA1 (rabbit anti-human ...
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bioRxiv - Cell Biology 2024Quote: ... or two siRNAs targeting human PLA2G15 (ThermoFisher s24296, s24297) using RNAiMax (ThermoFisher ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the human σ2 receptor was cloned into pcDNA3.1 (Invitrogen) mammalian expression vector with an amino-terminal protein C tag followed with a 3C protease cleavage site and transfected into Expi293 cells (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... for human HTT and Alexa fluor 647 (Thermofisher # B40958) for mouse Htt ...
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bioRxiv - Neuroscience 2024Quote: ... AT8 human PHF-Tau (Thermo Scientific, MN1020, 1:250). The following secondary antibodies were used ...
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bioRxiv - Cancer Biology 2024Quote: ... rabbit anti-human CD3 (clone SP7, Thermo Fisher Scientific); sheep anti-human CD34 (#AF7227-SP ...
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bioRxiv - Bioengineering 2024Quote: Human dermal fibroblast (HDF) cells (C0135C; ThermoFisher Scientific, UK) were cultured in flasks with Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Microbiology 2024Quote: ... Goat anti-human IgG (H+L) (Thermo Fisher Scientific) pre-coupled to Alexa Fluor 647 were used as secondary antibodies in flow cytometry experiments ...
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bioRxiv - Developmental Biology 2024Quote: ... in 2 mL complete human medium (DMEM/F12 [Gibco, Massachusetts ...
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bioRxiv - Immunology 2024Quote: ... Dynabeads™ Human T-Activator CD3/CD28 (ThermoFisher Scientific) were added to the culture ...
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bioRxiv - Microbiology 2024Quote: ... Goat anti-human IgG (H + L) (Thermo Fisher Scientific) or Goat anti-Human IgG Fc recombinant (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Human PA-1 cells were cultured in MEM (Gibco) supplemented with GlutaMAX ...
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bioRxiv - Neuroscience 2024Quote: ... human astrocytes at P4 (K1884, Thermo Fisher Scientific Gibco) were seeded in each well ...
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bioRxiv - Neuroscience 2024Quote: ... human astrocytes at P4 (K1884, Thermo Fisher Scientific Gibco) were seeded in each well ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... (Telangana, India) with human suspension hepatocytes (HMCS1S, Gibco USA). 200 µL of cell suspension containing 2 x 106 hepatocytes/mL (>95% viability ...
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bioRxiv - Cell Biology 2024Quote: ... primary human islets were first dissociated with TrypLE (Gibco) prior to incubation with a mouse monoclonal anti-human NTPDase3 antibody (Ectopeptidases ...
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bioRxiv - Microbiology 2024Quote: ... or Goat anti-Human IgG Fc recombinant (ThermoFisher Scientific) antibodies were pre-coupled to Alexa Fluor 647 and used as secondary antibody in flow cytometry experiments ...
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bioRxiv - Cancer Biology 2021Quote: ... which were then transfected with a combination of PG13-luc assay plasmid and a control plasmid pRL-SV40P (50:1) using Lipofectamine 2000 (Invitrogen, USA) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... guide RNA plasmid and a plasmid expressing SpCas9 at a ratio of 2:1:1 (total 12ug) using Neon electroporator (Life Technologies) and a 30ms ...
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bioRxiv - Cell Biology 2021Quote: ... hTERT-RPE1 cells were transfected with donor plasmid and AIO plasmid with the appropriate gRNAs using a Neon transfection system (Thermofisher Scientific). For 5 × 106 cells ...
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Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying essential circRNAsbioRxiv - Molecular Biology 2020Quote: ... Clones were scraped off the LB plates and plasmid DNA was extracted using PureLink™ HiPure Plasmid Maxiprep Kit (ThermoFisher, K210007). The libraries were submitted for next generation sequencing to confirm the coverage and diversity of gRNA and shRNA libraries ...
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bioRxiv - Developmental Biology 2020Quote: ... The next day PcDNA 3.1/ Twist1-BirA*-HA plasmid or PcDNA 3.1/ GFP-BirA*-HA plasmid was transfected into cells using Lipofectamine® 3000 (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... RPE-1 wildtype cells were transfected with the CRISPR/Cas9 plasmid containing the PCNA gRNA and the donor plasmid containing the K164R mutation using the Neon Transfection System (Invitrogen MPK5000) following standard protocols ...
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bioRxiv - Immunology 2021Quote: ... were transformed with the modified transfer vectors and before plasmid DNA extraction using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 1.5 μg of reporter plasmid and 15 ng of Cre-plasmid was mixed with 3 μL Lipofectamine LTX reagent (Invitrogen, #15338100), 1.5 μL PLUS reagent and 100 μL Opti-MEM following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2019Quote: ... This plasmid consists of six inserts and the plasmid cloning vector CloneJet pJET1.2/blunt (2974 bp; Thermo Fisher Scientific, Massachusetts, USA). For the inserts ...
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bioRxiv - Microbiology 2019Quote: Genomic DNA was extracted using the GeneJET Genomic DNA Purification Kit while plasmid DNA was extracted using the GeneJET Plasmid Miniprep Kit (Thermo Scientific). PCR assays were performed using Phusion High-Fidelity PCR Master Mix (Thermo Scientific) ...
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bioRxiv - Genomics 2019Quote: ... were used for sequencing the full length of cloned PCR product after plasmid purification using the GeneJET Plasmid Miniprep Kit (Thermo Scientific).
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bioRxiv - Bioengineering 2021Quote: ... nanobody sequences were PCR amplified with primers containing terminal regions of homology for cloning into a pRset plasmid (Addgene plasmid 3991)(Invitrogen, V35120) in frame and linked to mCherry ...
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bioRxiv - Neuroscience 2021Quote: ... Transient overexpression of VDR in SH-SY5Y cells was performed by transfecting a VDR expression plasmid (pcDNA3-VDR) or a mock control plasmid (pcDNA3) with Lipofectamine 2000 (Invitrogen, USA). For mammalian two-hybrid assays to assess the interaction between VDR and RXR ...
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bioRxiv - Systems Biology 2020Quote: ... We added 800–1000 ng of plasmid (SF2(gDNA) or SF2(cDNA) or SynT) using the Lipofectamine LTX plasmid transfection reagent (ThermoFisher Scientific) and changed the culture media to Opti-MEM™ Reduced Serum Medium (ThermoFisher Scientific) ...
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bioRxiv - Systems Biology 2020Quote: ... We added 800–1000 ng of plasmid (SF2(gDNA) or SF2(cDNA) or SynT) using the Lipofectamine LTX plasmid transfection reagent (ThermoFisher Scientific) and changed the culture media to Opti-MEM™ Reduced Serum Medium (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2021Quote: ... 1 million cells in 6-well plates were transfected with 2 μg of plasmid pT2_FF together with 2 μg of plasmid pCMV(CAT)T7-SB100x and 2 μg of plasmid pcDNA3.1-mCherry using Lipofectamine 2000 (Thermo Fisher, #11668027). After 24 h ...
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bioRxiv - Molecular Biology 2020Quote: ... K562#17 cells were transfected with 32 μg of barcoded IPR-PB-BC plasmid library and 6 μg of PB transposase plasmid using Lipofectamine 2000 (ThermoFisher #11668019). Mock-transfected (without PB transposase ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were extracted with NucleoSpin Plasmid Transfection-grade (Machery-Nagel) and concentration were measured on Nanodrop ND-1000 (Thermo Fisher Scientific). All plasmids were sequenced by Sanger method (Giga-Genomics).
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected with 1,250 ng of dCas13a m6A editor plasmid and 1,250 ng of crRNA plasmid using 5 μl of Lipofectamine 3000 (Thermo Fisher Scientific) in Opti-MEM I Reduced Serum Media (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected with 1,250 ng of dCas13a m6A editor plasmid and 1,250 ng of crRNA plasmid using 5 μl of Lipofectamine 3000 (Thermo Fisher Scientific) in Opti-MEM I Reduced Serum Media (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... The cDNAs were cloned into pDONR221 plasmid and introduced into the PB-Hyg-Dest plasmid using Gateway technology (Thermo Fisher Scientific). The PB-Hyg-Dest-mCherry-PAR-6B or the PB-Hyg-Dest-LifeAct-Ruby plasmid was co-transfected with the piggyBac plasmid using Lipofectamine 3000 to generate Hygromycin B resistant stable cell lines ...