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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Plasmid DNA from the overnight cultures was harvested via extraction with the PureLinkTM HiPure Plasmid Filter Midiprep kit (Thermo Fisher Scientific), and insert sequence was confirmed by Sanger sequencing at the on-site DNA Sequencing Facility (Dept ...
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bioRxiv - Biochemistry 2022Quote: ... The constructs were transformed into competent DH5α (E. coli) cells and plasmids were extracted using GeneJET plasmid miniprep kit (Thermo Scientific) to verify sequence integrity by sequencing (Eurofins Genomics) ...
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bioRxiv - Neuroscience 2022Quote: ... we cloned sequences encoding each BoNT light chain (from plasmids shared by Matt Kennedy, University of Colorado, USA) into the gateway vector donor plasmid (ThermoFisher, # K240020). We then transferred these sequences into the final pUASt destination vector from Carnegie Drosophila Gateway Vector Collection using the Gateway reaction kit (Thermofisher ...
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bioRxiv - Cancer Biology 2022Quote: ... target plasmid was transfected together with psPAX2 and pMD2.G packaging plasmids into 293T cells using lipofectamine 2000 transfection reagent (Invitrogen, 11668027). Media was changed 24h later and lentivirus supernatant was collected after an additional 24 h ...
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bioRxiv - Molecular Biology 2021Quote: ... BHK-21 cells were transfected 24 h prior to pseudotype inoculation with either empty plasmid or ACE2 expression plasmid (0.1 μg/well) using Lipofectamine LTX (Thermo Fisher Scientific). In order to study the antiviral activity of Camostat mesylate ...
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bioRxiv - Cell Biology 2019Quote: Stable cell lines were generated by co-transfecting the plasmid of interest together with a plasmid carrying the resistance gene for hygromycin with Cellfectin II (Life Technologies), and the selection process was started 2 d after transfection by supplementing the media with 250 μg/ml Hygromycin B.
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bioRxiv - Developmental Biology 2021Quote: ... a transposase expressing plasmid and a TOL2 plasmid expressing mCherry (as a transfection control) following the Lipofectamine 2000 protocol (Life Technologies). 48 hours post transfection ...
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bioRxiv - Cancer Biology 2019Quote: Lentiviral vector system includes the pLEX-MCS plasmid and the PMD2G and psPAX2 packaging plasmids (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Genetics 2020Quote: ... HEK293T cells were seeded on 48-well plates and transfected with SlugABEmax/SlugBE4max plasmid (300 ng) and gRNA plasmid (200 ng) using 1 ul of Lipofectamine 2000 (Life Technologies) in Opti-MEM(Gibco ...
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bioRxiv - Genetics 2020Quote: ... cells were seeded on 48-well plates and transfected with Cas9 plasmid (300 ng) and gRNA plasmid (200 ng) using 1 ul of Lipofectamine 2000 (Life Technologies) in Opti-MEM according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: The inducible XIST constructs within the HT1080 2-3-0.5a cells were modified by transiently transfecting two unique gRNA plasmids (pSPgRNA) and a Cas9 plasmid providing transient puromycin resistance (pSpCas9(BB)-2A-Puro) using Lipofectamine 3000 (ThermoFisher #L3000008). The day before the transfection the HT1080 cells were split into 24 well plates ...
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bioRxiv - Immunology 2020Quote: ... The 3T3 cells were transfected with Fsp I linearized CD40L plasmid (a kind gift from G. Freeman21,22) and Pvu I linearized pcDNA3-Neomycin plasmid) using Lipofectamine 2000 Reagent (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were co-transfected with sleeping beauty transposon plasmid pSBi-Hyg-BFP (hTMEM120A cDNA) and sleeping beauty transposase plasmid pCMV (CAT) T7-SB100 with Lipofectamine2000 (Life Technologies). Three days after transfection ...
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bioRxiv - Genetics 2022Quote: ... Plasmids and packaging plasmids psPAX2 and pMD.2G were co-transfected into cells using Lipofectamine™ 3000 Transfection Reagent (Invitrogen, L3000015). The medium was changed after 12 hour incubation ...
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bioRxiv - Neuroscience 2022Quote: ... and plasmids were purified 18 h after the inoculation with a 96-well plasmid purification kit (PureLink Pro, Thermo Fisher Scientific) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Approximately two million colonies were scraped from plates and pooled and plasmid DNA was purified using PureLink® HiPure plasmid filter Midiprep kit (Invitrogen). The resulting plasmid library was transformed into TRy1392 pdr5∆ yeast strain ...
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bioRxiv - Microbiology 2024Quote: ... transfections were performed by introducing 20 μg of targeting plasmid and 60 μg of linearised repair template (purified using Pure-Link™ HiPure Plasmid Midiprep Kit, Invitrogen) into ∽108 Percoll-enriched schizonts by electroporation using an Amaxa 4D Nucleofector X (Lonza) ...
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bioRxiv - Developmental Biology 2024Quote: ... 293 cells were transfected with plasmids encoding His-tagged recombinant proteins as described in the Plasmids section using lipofectamine 2000 (Invitrogen, #11668019) following the manufacturer’s instructions (four 100 mm plates each plasmid) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1.5 μg dual sgRNA plasmids or 1 μg sgRNA plasmids and 1 μg homologous recombination donor using Neon™ NxT Electroporation System (Invitrogen). After 48 hr ...
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bioRxiv - Immunology 2024Quote: ... 18 μg of pMDLg/p.RRE Gag/Pol plasmid and 18 μg of pRSV.Rev plasmid alongside 15 μg of transfer vector plasmid encoding for CAR of interest using Lipofectamine 2000 (Thermo Fisher Scientific) and Opti-MEM (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 µg of each plasmid (gagpol, vsv-g, and transfer plasmid) were combined with 2 ml Opti-MEM I Reduced Serum Medium (Gibco 31985070) and 90 µl MirusBio TransIT-LT1 Transfection Reagent (Mirus MIR2306 ...
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bioRxiv - Biophysics 2024Quote: ... HEK293T cells were co-transfected with 200 ng pCAGEN plasmid for TDP-43 and 1000 ng pCAGEN plasmid for the other protein using Lipofectamine 3000 Transfection Reagent (Invitrogen, L3000001) in 12-well plates ...
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bioRxiv - Immunology 2024Quote: ... pMDLg/p.RRE (18 μg) packaging plasmids and 15 μg of expression plasmids were mixed and transfected into 293T cells using Lipofectamine 3000 (Invitrogen #L3000150) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: The HEK293T packaging cells were transfected with OSA-expressing plasmids with lentiviral packaging plasmids using Lipofectamine Reagent 2000(Thermo Fisher Scientific). After 48 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... the SunTag plasmids and HMGB1-BFP (2 dishes) or Smad3-BFP plasmid (2 dishes) were simultaneously transfected with Lipo3000 transfection reagent (ThermoFisher Scientific). One dish of cells transfected with HMGB1-BFP ...
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bioRxiv - Molecular Biology 2023Quote: Extrachromosomal arrays were introduced by injection of 10 ng/μl plasmid DNA purified using the PureLink™ Quick Plasmid Miniprep Kit (Invitrogen) into the gonad of 1 DOA IR1284 (zcIs14[myo-3p::mtGFP] ...
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bioRxiv - Microbiology 2023Quote: ... The following day each well was co-transfected with 1 µg of replicon-encoding plasmid and 3 µg of C-prM-E-encoding plasmid using Lipofectamine 3000 (Cat# L3000-015; ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... were co-transfected with plasmids encoding GFP-nsP3 fusions and the integrase-encoding plasmid using Lipofectamine™ 3000 according to the manufacturer’s recommendations (ThermoFisher Scientific). Clones of blasticidin-resistant ...
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bioRxiv - Genomics 2023Quote: ... cells at 80–90% confluency were co-transfected with 0.25 µg of single-sgRNA (or 4sgRNA) plasmids and 0.25 µg of dCas9-VPR plasmids using Lipofectamine 3000 (Thermo Fisher Scientific). Three days post-transfection ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 5 ml of the culture was pelleted by centrifugation and plasmid DNA was isolated using a GeneJET Plasmid Miniprep Kit (Thermo Scientific). The plasmid sequence was verified using BigDye 3.1 sequencing chemistry and an ABI3130 Genetic Analyzer ...
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bioRxiv - Molecular Biology 2023Quote: ... was used at a 3:1 ratio with 1 µg total plasmid (500 ng nLuc plasmid + 500 ng pGL4.13) in 100 µL in Opti-MEM (Thermo Fisher # 31985062). For 96-well plates ...
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bioRxiv - Cell Biology 2023Quote: ... co- transfected with lentiviral packaging plasmids (pCEP4-tat, pHEF-VSVG and pNHP) and lentiviral expression plasmids using Lipofectamine 2000 (Invitrogen, 11668019) as previously described50 with the following modifications ...
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bioRxiv - Developmental Biology 2023Quote: SOX2:GFP ESCs were plated into a gelatinised 6-well plates in SL and lipofected with the linearized Gata6:mCherry-Hygromycin plasmid and the CRISPR plasmid using Lipofectamine2000 (ThermoFisher 11668019) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The mESCs were co-transfected with the 1.66 μg of the two sgRNA nickase-Cas9 plasmids and 0.83 μg of the HDR plasmid using the Lipofectamine LTX and Plus Reagent (Invitrogen, 15338-100) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The mESCs were co-transfected with the 1.66 μg of the two sgRNA nickase-Cas9 plasmids and 0.83 μg of the HDR plasmids using the Lipofectamine LTX and Plus Reagent (Invitrogen #15338-100). Transfected cells were first selected by 1 μg/ml puromycin for 2 days and subsequently selected by 250 μg/ml hygromycin for 6 days ...
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bioRxiv - Cancer Biology 2023Quote: Lentiviral packaging plasmids (pCMV-D8.9 and pCMV-VSVG) and reporter plasmids were transfected into HEK-293T cells using Lipofectamine 3000 (ThermoFisher cat#L3000001). Virus-containing supernatant was harvested at 48 and 72 hours after transfection and viral particles were concentrated with Lenti-X Concentrator according to the manufacturer’s instructions (Takara Bio cat#631232) ...
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bioRxiv - Microbiology 2023Quote: Plasmid DNA from either wild type dengue plasmid or mutated dengue plasmid were quantified using a High Sensitivity Range Qubit (Thermo Fisher). Library preparation was carried out with the Nextera XT DNA Library Prep kit (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... HD patient iPSC-derived astrocytes were transiently transfected with 2 μg hFAN1 plasmid and control plasmid (pTT5 vector) by Lipofectamine 3000 (Invitrogen; L3000008) every week ...
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bioRxiv - Biochemistry 2024Quote: ... 300 ng of WT or CNNC mutant miRNAs with 50 ng of non-CNNC mir-24 plasmids) and Drosha plasmids (800 ng per well) was performed in 12-well cell culture plates using Lipofectamine2000 (Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... COS-7 cells were transfected with reporter plasmid construct together with pRL-TK Renilla luciferase plasmid by Lipofectamine™ 2000 (Invitrogen).
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bioRxiv - Molecular Biology 2024Quote: ... transfer plasmids were co-transfected with GAG/POL and VSVG plasmids into 293T cells using Lipofectamine™ 3000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... The co-culture was transfected with the 8-plasmid set (2 μg of each plasmid) using Lipofectamine 2000 transfection reagent (Fisher Scientific). At 24 h post transfection ...
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bioRxiv - Synthetic Biology 2024Quote: ... Libraries were aliquoted in 10 mL (OD600 ∼ 0.2) and re-grown for 2-3 h to extract plasmids using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) for sequencing and for subcloning into the expression strain ...
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bioRxiv - Molecular Biology 2024Quote: ... transfer plasmids were co-transfected with GAG/POL and VSVG plasmids into 293T cells using Lipofectamine™ 3000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... Lysates and positive technical controls (Human Universal Reference RNA - uhrRNA Agilent Cat# 740000, Santa Clara, California and Human Brain Total RNA brRNA - ThermoFisher AM7962, Waltham, Massachusetts), as well as no-cell negative controls (1X TempO-Seq lysis buffer alone ...
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bioRxiv - Immunology 2020Quote: The assay to measure total IgG was modified to measure IgG subclass responses at 1/100 including a two-step biotinylated anti-human IgG subclass (mouse anti-human IgG1: HP6069, IgG2: HP6002, IgG3: HP6050 IgG4: HP6023, Thermo fisher Scientific, UK) with a streptavidin-Phycoerythrin tertiary (Thermo fisher Scientific ...
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bioRxiv - Developmental Biology 2019Quote: ... Samples were run on the Clariom™ S Human Array to measure gene expression at >20,000 genes in the human genome (Affymetrix, Santa Clara, CA). Raw data generated from the arrays were read into R statistical software (version 3.5.1 ...
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bioRxiv - Cell Biology 2020Quote: ... Alexa Fluor™ 488-Human Transferrin conjugate (Cat# T13342) and DiI–human LDL conjugate (Cat# L3482) were obtained from Molecular Probes (Eugene, OR). Primer kits and Master Mix for RT-qPCR were purchased from Applied Biosystems (Foster City ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA levels were normalized to non-human primate β-actin (Table S1) or human β-actin (Hs99999903_m1, Applied Biosystems; Thermo Fisher Scientific) and calculated by the ΔΔCt method [46].
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bioRxiv - Microbiology 2021Quote: ... Viral RNA levels were normalized to non-human primate β-actin (Table S1) or human β-actin (Hs99999903_m1, Applied Biosystems; Thermo Fisher Scientific) and calculated by the ΔΔCt method [46].