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Citations for Thermo Fisher :
1651 - 1700 of 10000+ citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade D UG28R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... BODIPY (D-3922, Life Technologies, Waltham, MA). Insulin-Transferrin-Selenium (ITS -G ...
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bioRxiv - Immunology 2020Quote: ... Precipitated proteins were bound to 50:50 Protein A: Protein G Dynabeads (Invitrogen) for one to two hours and washed extensively with IP wash buffer (50 mM Tris pH 8 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were incubated with fast-flow protein G- or protein A-Dynabeads (Invitrogen) for 2 hours and centrifuged to eliminate nonspecifically bound proteins ...
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bioRxiv - Bioengineering 2022Quote: ... coliΔXbaI cell cultures are both induced with 0.5 mM (final concentration) of Isopropyl β-D-1-thiogalactopyranoside (IPTG; Invitrogen), and incubated at 37 °C in the dark for a given period of time before being disrupted ...
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bioRxiv - Microbiology 2020Quote: ... and resuspended in fresh DMEM-10 (Gibco DMEM #11995-065 with 4.5 g/L D-Glucose and 110 mg/L Sodium Pyruvate, 10% Fetal Bovine Serum [HyClone], 1% HEPES [Gibco 1M 15630-080] ...
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bioRxiv - Cell Biology 2020Quote: ... referred to herein as INS-1 832/13) were cultured in RPMI 1640 medium with 11.1 mM D-glucose (Invitrogen) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2022Quote: ... were cultured adherently in DMEM (4.5 g/L D-Glucose) supplemented with 1× MEM NEAA (catalog no. 11140-035, Gibco), 1× GlutaMAX (catalog no ...
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bioRxiv - Neuroscience 2021Quote: ... a staining solution of 1 mg/mL 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside (X-gal, Invitrogen), 1× citratesodium phosphate buffer (pH 6.0) ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then induced by the addition of 0.5 mM Isopropyl B-D-1-thiogalactopyranoside (IPTG, INVITROGEN Life Technologies) and further grown at 21°C for 16-18 h ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then induced by the addition of 0.5 mM Isopropyl B-D-1-thiogalactopyranoside (IPTG, INVITROGEN Life Technologies) and further grown at 21°C for 16-18 h ...
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bioRxiv - Neuroscience 2019Quote: ... Plasmids pCAG-dsRed and Pak3K297R transfection was performed at 7 d in vitro (DIV) with 1 µg of DNA and 1.5 µl of Lipofectamine (Invitrogen), as described in manufacturer’s manual (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: Prion-infected brain homogenate was prepared by homogenizing 200 brains from CD-1 mice terminally-infected with the RML prion strain in Dulbecco’s phosphate buffered saline (D-PBS; Gibco) to produce a pool of ~1 litre 10 % (w/v ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK 293T and Flp-In HEK293 T-REx were maintained in DMEM-GlutaMAX at 4.5 g*ml-1 D-glucose (Gibco) supplied with sodium-pyruvate ...
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bioRxiv - Cancer Biology 2020Quote: ... U2OS and HEK-239T cells were grown in DMEM containing 2.5 mM L-glutamine and 4.5 g l-1 D-glucose (Life Technologies). H1299 cells were grown in RPMI 1640 medium containing 2.5 mM L-glutamine.
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bioRxiv - Immunology 2021Quote: ... CCR10 (248918) from R&D, CD11a (M17/4) from eBioscience, FcεRI (MAR-1), c-Kit (CD117, 2B8) from Invitrogen and Mouse BD Fc Block™ ...
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bioRxiv - Immunology 2022Quote: ... BCRJ 0234) cell lines were maintained at 37ºC and 5% CO2 in DMEM (1 g/L D-glucose, GIBCO) supplemented with 20% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... and 1% n-Dodecyl-β-D-maltoside were loaded onto Novex 4-16% Bis/Tris gradient gels (Life Technologies). When assaying stoichiometry of AMPAR-auxiliary subunit complexes ...
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bioRxiv - Cancer Biology 2023Quote: ... Anti-NKp46 and NKp30 agonist antibodies (R&D) were coated (1 µg/mL) in NUNC 96-well plates (Thermofisher) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... their columns were excised in a dissection medium constituted of a solution of D-glucose 6.5 mg ml-1 in DPBS (Gibco; Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... were grown on 10-mm coverslips previously coated with poly-D-lysine (0.1 mg/mL, 1 h, 37 °C, Gibco) placed in a well on a 24-well plate ...
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bioRxiv - Biophysics 2023Quote: ... We used carboxy-terminated microspheres that exclusively were found to enter inside the condensates (d = 1 µm, Thermofisher, F8816).
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bioRxiv - Bioengineering 2024Quote: A total of 5 × 103 Hek293T and HeLa cells were put into individual wells containing Dulbecco’s Modified Eagle Medium (D-MEM) [containing 10% Fetal Bovine Serum (FBS) and 1% Antibiotic-Antimycotic solution (Gibco)] ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were cultured in Dulbecco’s Modified Eagle Medium with 1 g/L D-glucose (low-glucose DMEM; Invitrogen, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2022Quote: ... were incubated with 100 ng/μL of mouse anti-human CD171 (L1CAM, neural adhesion protein) biotinylated antibody (clone 5G3, eBioscience/Thermo Fisher Scientific; Catalog # 13-1719-82); mouse anti-human GLAST (ACSA-1 ...
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bioRxiv - Cancer Biology 2020Quote: ... using Taqman probes (human RBM10: Assay ID, Hs00275935_m1, human Bcl-xL: Assay ID, Hs00236329_m1, Applied Biosystems, human Bcl-xS ...
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bioRxiv - Biochemistry 2022Quote: ... Human serum (catalog # 4522 from human male AB plasma) and fetal bovine serum were from Gibco, Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... and plasma human glucagon was measured by the human glucagon ELISA kit (Thermo Scientific, Frederick, MD). Exendin-4 plasma levels were measured using the exendin-4 EIA kit (Phoenix pharmaceuticals ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 50 μL fresh FACS buffer and 10 μL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Immunology 2022Quote: ... Total IgG in human sera was measured using a commercial human IgG ELISA kit (Fisher Scientific) according to manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... All human CD8+ T cells were activated with anti-human CD3/CD28 dynabeads (11131D, Thermo Scientific) at a 1:1 cell-to-bead ratio and cultured in complete RPMI supplemented with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... 100 ng/mL Human Recombinant IL-4 and 250 ng/mL Human Recombinant GM-CSF (ThermoFisher) for six days at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... Fragments of human CCDC22 and CCDC93 and full-length human DENND10 were ordered as GeneStrings (ThermoFisher) with the gene codon optimized for E ...
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bioRxiv - Cell Biology 2023Quote: ... Purified human neutrophils and T cells were cultured in Human Plasma-Like Medium (HPLM; ThermoFisher Scientific) with 5% heat inactivated FBS supplemented ...
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bioRxiv - Immunology 2023Quote: ... Primary human T cells were thawed and activated with Human T-Expander CD3/CD28 Dynabeads (Gibco) at a 3:1 bead:cell ratio in complete medium (RPMI 1640 supplemented with 10% fetal bovine serum ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 50 µL fresh FACS buffer and 10 µL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 50 µL fresh FACS buffer and 10 µL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Neuroscience 2023Quote: ... Secreted Aβ42 peptides were quantified using the Human Amyloid β1-42 human ELISA kit (Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: Fresh human BAL cells and frozen human PBMCs were stained with Aqua live/dead dye (Invitrogen) for 20min at room temperature ...
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bioRxiv - Genomics 2021Quote: ... 50μg isotope-matched IgG and 50μl protein A/G agarose beads (A:G = 1:1, ThermoFisher 15918014 and ThermoFisher 15920010) and rotated at 4℃ for 8h to preclear the chromatin ...
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bioRxiv - Genomics 2021Quote: ... 50μg isotope-matched IgG and 50μl protein A/G agarose beads (A:G = 1:1, ThermoFisher 15918014 and ThermoFisher 15920010) and rotated at 4℃ for 8h to preclear the chromatin ...
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bioRxiv - Microbiology 2020Quote: ... and proteins detected using specific primary (diluted at 1:1000) and HRP-secondary antibodies (ThermoFisher, diluted at 1:10,000). Protein bands were detected using Pierce SuperSignal West Pico chemiluminescent substrate kit (Pierce ...
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bioRxiv - Systems Biology 2020Quote: ... 1 mM PMSF) and precleared for 1 hour at 4°C with 25 μl of protein G Dynabeads (Invitrogen). The precleared chromatin solution (1.5 × 106 cells ...
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bioRxiv - Cell Biology 2022Quote: ... medium was replaced to low serum RPMI (RPMI-1640 supplemented with 1% HS and 1% FBS) with 50 ng/ml NGF 2.5S Native Mouse Protein (Gibco) to differentiate cells for desired time ...
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bioRxiv - Genomics 2020Quote: ... The DNA was captured on a 1:1 mixture of protein A and G Dynabeads (Invitrogen 10001D and 10003D). After washes ...
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bioRxiv - Cancer Biology 2020Quote: ... TIC-enriching 3-D cultures (3-D) were maintained in stem cell media: DMEM:F12 (+ L-glutamine, + 15 mM HEPES) (Gibco) supplemented with 1% penicillin/streptomycin ...
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bioRxiv - Cancer Biology 2021Quote: ... The silencer select siRNA targeting human ERK3 and the Silencer non-targeting Control #1 were purchased from Ambion.
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bioRxiv - Cancer Biology 2021Quote: Cells (1-1.5×10^6) were incubated with FACS sample buffer (DPBS, 0.5% FBS) and human FC Block (Invitrogen) for 20 minutes on ice followed by incubation at 4°C for 30 minutes with the following - antibodies ...
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bioRxiv - Genomics 2020Quote: ... LECs were stained with probes designed to target human LETR1 (Type 1 Probe, VA1-3018146, Thermo Fisher Scientific), human Malat1 (positive control ...
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Targeting MOG to skin macrophages prevents EAE in macaques through TGFβ-induced peripheral tolerancebioRxiv - Immunology 2019Quote: ... Bound cynomolgus monkey antibodies were detected with alkaline phosphate-labeled goat-anti-human IgG (1:1000, 4HI1305, Invitrogen Life Technologies ...
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bioRxiv - Microbiology 2019Quote: ... and human monocytic leukemia (THP-1) cells (TIB-202; ATCC) cell lines were maintained in RPMI medium (Gibco) supplemented with 10% fetal bovine serum (FBS ...