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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... The gels contained a thin top layer (10 μm) impregnated with 1 μm fluorescent beads (FluoSperes; Molecular Probes) that acted as fiduciary markers of substrate deformation ...
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bioRxiv - Bioengineering 2021Quote: ... the cultures were exposed to appropriate fluorescent secondary antibodies (1/500, ThermoFisher, Alexa-488, Alexa-568, Alexa-647) in 4% horse serum for 2 hr at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... VSMCs were seeded onto 12 kPa hydrogels containing 0.5 µm red fluorescent (580/605) FluoSpheres (1:1000) (Invitrogen). Imaging was performed using a Zeiss Axio Observer live cell imaging system that captured 20x magnification images every 2 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... Lipid mixes contained a small amount (1 mol%) of the fluorescent lipid probe pTexas-Red DHPE (Thermo Fisher). 2 μl of the lipid mix was spread with a glass syringe on a PEGylated glass coverslip ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated with a fluorescent secondary antibody (donkey anti-rabbit Alexa Fluor 488-conjugated, Invitrogen, 1/500) in 1% donkey serum ...
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bioRxiv - Neuroscience 2021Quote: ... followed by fluorescent-conjugated secondary antibodies and counterstained with Hoechst 33342 (ThermoFisher 62249, 1 μg/mL in PBS). F-actin was labelled prior to cell fixation using Phalloidin-647 (Abcam ab176759) ...
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bioRxiv - Biophysics 2022Quote: ... Rather than purified sensor,20 μl of a 1/10,000 dilution of carboxylate latex fluorescent beads (Fluospheres, Invitrogen) of either 20 nm (F-8786) ...
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bioRxiv - Microbiology 2020Quote: Phagocytosis assays were performed in vivo as previously described 8,32 using red fluorescent FluoSpheres (1 μm Molecular Probes) at a 1:10 dilution in 1x PBS ...
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bioRxiv - Biophysics 2022Quote: ... The nuclei were stained by incubating the cells with PBS containing fluorescent secondary antibodies (1:1,000, A21207, Invitrogen) and DRAQ5™ (1:1,000 ...
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bioRxiv - Microbiology 2022Quote: ... Host appropriate secondary antibodies were labeled with a red fluorescent dye Alexa Flour 594 (Life Technologies; 1:300) or biotinylated secondary antibody (Vector Laboratories ...
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bioRxiv - Systems Biology 2023Quote: RNA was reverse-transcribed from annealed fluorescent primer in a reaction containing 1× First Strand Buffer (Thermo Fisher), 5 mM DTT ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then washed with PBS and incubated in Alexa Fluor-conjugated fluorescent secondary antibodies (Invitrogen; 1:500) for 2 hr at room temperature ...
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bioRxiv - Physiology 2022Quote: ... then incubated for 2 hours at room temperature in Alexa Fluor fluorescent secondary antibody (Life Technologies; 1:500) prepared in blocking solution ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubation with a 1:20 000 dilution of a fluorescent-dye-coupled secondary antibody (Thermofisher Scientific) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... followed by overnight incubation with fluorescent secondary antibodies (Fluor 568 labeled donkey anti-Mouse (1:500; Life Technologies), Fluor 488 labeled donkey anti-rabbit (1:500 ...
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bioRxiv - Biophysics 2024Quote: Larval muscles were stained with fluorescent nanobodies (Loreau et al., 2023) and rhodamine-phalloidin (1:1000 Molecular Probes) for 2 hr at RT or overnight at 4 °C as previously described in detail (Loreau et al. ...
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bioRxiv - Neuroscience 2024Quote: ... samples were incubated in fluorescent secondary antibody solutions: goat anti-mouse Alexa Fluor 488 (1:500; Life Technologies) and goat anti-rabbit Alexa Flour 555 (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... Live cultures or thawed cells were either expanded as floor plate progenitors (following Brundin approach; Floor Plate Progenitor Kit expansion protocol (ThermoFisher; A3165801) or terminally differentiated to DA neurons with all prior factors except in neurobasal base medium after day 25.
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bioRxiv - Immunology 2023Quote: ... A 10 μl supernatant sample was mixed with 90 μl substrate solution containing 0.5 mg/ml of 4-methylumbelliferyl N,N’-diacetyl-β-D -chitobioside in an assay buffer provided as part of the kit and in a 96-well plate (black/clear bottom plate, Thermo Fisher). The reaction was incubated at 37 °C for 30 min and then stopped by adding 100 μl stop solution ...
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Targeting Noradrenergic Neurons of the Locus Coeruleus: A Comparison of Model Systems and StrategiesbioRxiv - Neuroscience 2022Quote: ... containing primary antibodies (rabbit against tyrosine hydroxylase, 1:1000, AB152, Merck Millipore; and chicken against green-fluorescent protein, 1:750, A10262, Invitrogen, Thermo Fisher Scientific) to allow for sufficient target protein-primary antibody interaction ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were then rinsed in TBS and stained with a fluorescent Nissl (1:500, 1 hr; NeuroTrace™; N21479, 435/455nm, Thermo Fisher Scientific). Slides were then coverslipped with Vectashield hardset antifade mounting medium with a DAPI counterstain (H-1500-10 ...
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bioRxiv - Plant Biology 2022Quote: ... Leaves from T0 regenerants bearing a fluorescent reporter were stained with Propidium Iodide (PI) (1:100 dilution of 1 mg/ml stock Molecular Probes; Invitrogen detection technologies) and visually inspected using confocal microscopy for expression of the reporter as confirmation of successful transformation ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 2 h at RT with appropriate fluorescent secondary antibodies diluted in PBS – 1% NGS: goat anti- rabbit Alexa Fluor® 488 (1/1000, Invitrogen, A11008, RRID:AB_143165) or goat anti-rabbit Alexa Fluor® 568 (1/1000 ...
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bioRxiv - Plant Biology 2019Quote: ... or pDEST-24 carrying a C-terminal GST purification tag (Thermo Fisher Scientific, Hampton, NH, USA).
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bioRxiv - Cell Biology 2021Quote: ... Antibodies were purified using GST-Bro1ΔPRR immobilized on an AminoLink column (Thermo Fisher Scientific, Waltham, MA) and verified for sensitivity and specificity using yeast extracts (SEY6210 and bro1Δ ...
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bioRxiv - Cell Biology 2023Quote: GST-IRE1αLD (S24-D443) was expressed from pGEX-TEV vector in BL21(DE3) pLysS E.coli (Invitrogen). Expression was induced in log phase cultures (OD600 = 0.6-1.0 ...
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bioRxiv - Neuroscience 2020Quote: ... To produce a suspension of fluorescent microspheres adequate for this purpose 1.2μl of stock solution of 0.5μm fluorescent microspheres (Invitrogen Tetraspeck 0.5μm – Cat No. T7281) was added to 100μl of molten agarose (1.2% w/v ...
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bioRxiv - Bioengineering 2019Quote: Fluorescent beads: In this study we used Yellow/Green fluorescent nanoparticles with a diameter of 100 nm (FluoSpheres, ThermoFisher Scientific, USA).
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bioRxiv - Developmental Biology 2022Quote: Fluorescent in situ hybridization was performed according to standard protocols adding a secondary fluorescent antibody (anti-goat Alexa555, Invitrogen A-21432); cv-c riboprobe was marked using DIG RNA Labelling Kit (Roche ...
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bioRxiv - Genetics 2019Quote: ... ExpiCHO-S cells were transfected with full-length pRTL1-GCSF vectors using the ExpiFectamine™ CHO Transfection Kit (Life Technologies Corporation ...
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bioRxiv - Microbiology 2020Quote: ... according to the manufacturer’s instructions and then quantified on the Qubit 3.0 fluorometer using the DNA high sensitivity assay kit (Thermo-Fisher Scientific). The consortium metagenomic library was prepared with 200 ng of starting material using the TruSeq DNA Nano kit (Illumina ...
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bioRxiv - Genetics 2021Quote: ... The hGOT1-GFP and mutant hGOT1-GFP constructs were transfected into Chinese Hamster Ovary (CHO) (ExpiCHO-S) cells with an ExpiFectamine Transfection Kit (Gibco) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Plasmid pCAG-CD5-S-ecto-6P-Spytag was transiently transfected into ExpiCHO cells using ExpiFectamine CHO transfection kit (Thermo Fisher). After 18-22 hr of transfection ...
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bioRxiv - Pathology 2022Quote: ... using the DNA Tissue Kit S (QuickGene, Kurabo, Japan) according to the manufacturer’s instructions with modifications: RNA carrier (Applied Biosystems, Thermo Fisher Scientific Waltham ...
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bioRxiv - Immunology 2019Quote: ... The full length and truncation sequences were transferred into a Gateway compatible destination vector that includes an N terminal triple-epitope tag (S protein tag, FLAG epitope tag and Streptavidin-binding peptide tag) using the LR clonase enzyme kit (ThermoFisher). Plasmids were transfected into 293T cells using FuGENE transfection reagent (Promega ...
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bioRxiv - Microbiology 2022Quote: ... or the NucleoMag VET kit (Machery-Nagel) according to the manufacturer‟s guidelines on a Kingfisher Flex Purification system (Thermofisher). Extracted RNA was assessed using the TaqPath COVID-19 CE-IVD RT-PCR Kit (Thermofisher ...
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bioRxiv - Developmental Biology 2023Quote: ... Human SORCS2 mRNA was transcribed from a pcDNA3.1/zeo-SorCS2A-P716 plasmid (provided by Peder S. Madsen, Aarhus Univeristy) with mMessage mMachine T7 Transcription kit (Thermo Fisher) and purified with RNA MinElute kit (Qiagen).
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bioRxiv - Bioengineering 2023Quote: ... The sample’s DNA was extracted using phenol-chloroform and kept at −20°C until applying the PicoGreen dsDNA assay kit (Invitrogen). Total sGAGs of the samples were obtained via papain (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... formaldehyde-fixed paraffin-embedded sections were detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) assay (ThermoFisher Scientific; C10619; Waltham, MA) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2022Quote: ... and poly(A) tailing to the 3’ end of the first-strand cDNA (by using terminal deoxynucleotidyl transferase - 10533-073, Invitrogen, final concentration : 0.75U/mL). After the second-strand cDNA synthesis ...
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bioRxiv - Cell Biology 2023Quote: ... The oligonucleotides were pooled and elongated overnight at 37°C with a ddUTP conjugated to a desired dye (Atto 633 for the YFP probe) using terminal deoxynucleotidyl transferase enzyme from Life Technologies (Thermo Fisher Scientific). The fluorescently labelled oligonucleotides were then purified by Oligo Clean & Concentrator kit (Zymo Research® ...
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bioRxiv - Developmental Biology 2021Quote: ... at 5 m/s for 30 s with 1.4 mm ceramic beads (15-340-153, Fisher Scientific, Waltham, MA, USA). HH37 and older lower jaws were homogenized at 5 m/s for 60 s with 2.8 mm ceramic beads (15-340-154 ...
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bioRxiv - Developmental Biology 2020Quote: ... at 5 m/s for 30 s with 1.4 mm ceramic beads (15-340-153, Fisher Scientific, Waltham, MA, USA). HH37 and older lower jaws were homogenized at 5 m/s for 60 s with 2.8 mm ceramic beads (15-340-154 ...
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bioRxiv - Biochemistry 2022Quote: ... The drug-selected recombinant CHO-S cells were adapted to serum-free suspension culture using CHO-S medium (CD Forti CHO; ThermoFisher). For large-scale cultures ...
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bioRxiv - Biochemistry 2020Quote: ... pcDNA3-myc-S or pcDNA3-S plasmids (500 ng DNA per ml of culture at 2 × 106 / ml) using Expifectamine (ThermoFisher). Cells were analyzed by flow cytometry 24 h post-transfection ...
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bioRxiv - Microbiology 2021Quote: ... or full-length S with two proline mutations (mRNA-S-2P) were synthesized by in vitro transcription using T7 RNA polymerase (MegaScript, Ambion) on linearized plasmid templates as previously reported [35] ...
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bioRxiv - Microbiology 2021Quote: ... VF-Hi and VF-Lo (generated from this study) were maintained in Dulbecco′s Modified Eagle′s Medium (DMEM) (Gibco) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2020Quote: ... HCoV-OC43-S or HCoV-HKU1-S C-terminally fused to the green fluorescence protein (GFP) using Lipofectamine 2000 (Invitrogen). Two days post transfection ...
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bioRxiv - Bioengineering 2020Quote: ... The grids were blotted for 2.5 s or 3.0 s and flash-frozen in liquid ethane cooled by liquid nitrogen with Vitrobot (Mark IV, Thermo Scientific). The cryo-EM samples were transferred to a Titan Krios operating at 300 kV equipped with Cs corrector ...
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bioRxiv - Cancer Biology 2023Quote: ... 4 times with 6 m/s and 2 times with 6.5 m/s intermitted by cooling on ice using MP Biomedicals™ Lysematrix D (ThermoFisher Scientific ...