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Citations for Thermo Fisher :
1651 - 1700 of 10000+ citations for 6 Methoxy 2 3 4 9 tetrahydro 1H b carbolin 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Total RNA was prepared from one lung lobe collected at 3 dpi using lysing matrix D (MP Biomedical) containing 1 ml of TRIzol reagent (Ambion, Life technologies) and homogenization at 20 s at 4.0 m/s twice using MP Biomedical Fastprep 24 Tissue Homogenizer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The transfection reagent was mixed with the pOG44 Flp-Recombinase expression vector and the pCDNA5/TO/FRT-3xMyc-eGFP-CLIP-170 WT or one of the mutant vectors with a 3:1 ratio in Opti-MEMTM I medium (Gibco, ThermoFisher Scientific). Selection started 48h after the transfection in a Dulbecco’s modified Eagle medium (DMEM ...
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bioRxiv - Plant Biology 2022Quote: ... The seedlings were screened by microscopy for YFP and then fixed and processed for immunofluorescence microscopy using a 1:2000 dilution of monoclonal anti-α-tubulin B-5-1-2 antibody (Life Technologies; 32-2500) followed by 1:2000 dilution of Alexa-568 goat anti-mouse antibody (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... and miR-9/9*-124 + TuD-miR-124 using TRIzol Reagent (Invitrogen, 15596026) in combination with RNeasy micro kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... and the bacterial pellets resuspended in PBS and supplemented with a 1:1 ratio of 2X LIVE/DEAD solution (6 μM SYTO-9 stain and 30 μM propidium iodide) from LIVE/DEAD® BacLight kit (Invitrogen). After incubation in the dark for 15 min ...
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bioRxiv - Cell Biology 2019Quote: ... RNA was isolated from whole eyes of 6-month old cep290 mutant and sibling control animals (n = 9 per group) with Trizol (ThermoFisher 15596026). Reverse transcription using 1 μg RNA was performed with the iScript cDNA Synthesis kit (Bio-Rad 1708891) ...
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bioRxiv - Cancer Biology 2021Quote: ... and mPRs (PAQR5, 6, 7, 8, 9) using Power SYBR Green Master Mix with ViiA 7 Real-Time PCR System (Applied Biosystems). RT-qPCR plates with various cell-lines were prepared using an epMotion 5075 automated liquid handling system (Eppendorf) ...
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Control of cortical cytoskeleton-membrane interaction by RhoA regulates peripheral nerve myelinationbioRxiv - Neuroscience 2021Quote: ... Teased fiber preparations were permeabilized in 0.25% TX-PBS for 30 min and blocked for 1h in 1% NGS/1% horse serum (Gibco)/1% HEPES/PBS ...
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bioRxiv - Immunology 2020Quote: ... and Fluo-4 AM (Invitrogen, 2 μM) probes for 30 min before fixation with 1% PFA for 4 hours ...
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bioRxiv - Genomics 2021Quote: ... 3.5× 10−4% 2-mercaptoethanol (Gibco, 21985023), 0.12% Nicotinamide (Calbiochem ...
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bioRxiv - Physiology 2020Quote: ... muscles were stained for 3 minutes with 10μM 4-(4-diethylaminostyrl)-N-methylpyridinium iodide (4-Di-2ASP, Molecular Probes) to allow imaging muscle with an upright epifluorescence microscope (Leica DMR ...
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bioRxiv - Cell Biology 2020Quote: ... media was changed gradually into a gradient of (1/4) Neurobasal media (NB: based medium contained 1% Lglutamine, 1% N2 and 2% B27 (all from Invitrogen)) plus reduced gradient of (3/4 ...
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bioRxiv - Cell Biology 2021Quote: ... anti-desmocollin 2 and 3 (clone 7G6, Thermofisher), 1:100 ...
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bioRxiv - Cell Biology 2022Quote: ... HSS117872 (Epsin-2) and HSS147867 (Epsin-3) (Invitrogen) on day 2 and 3 (Boucrot et al. ...
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bioRxiv - Cell Biology 2024Quote: ... Passaged every 2-3 days with TrypleE (Gibco).
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bioRxiv - Biochemistry 2023Quote: ... The DNA-transfection reagent complex was prepared by combining 4 µg of pcDNA3.1_Mb4.WT (hGOAT) or mutant plasmid and 9 µL Lipofectamine 2000 transfection reagent (Invitrogen) in a total volume of 500 µL supplement free DMEM followed by incubation for 30 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... and mixed 1:1 with B-PER reagent (Thermo Fisher Scientific). Cells were lysed by three passages through a French press at 138 MPa pressure ...
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bioRxiv - Immunology 2022Quote: ... 1% penicillin-streptomycin and 1/1000 b-mercapto-ethanol (Life Technologies) in 96- well U-bottom plates 24 hours prior to co-culture with the indicated combinations of growth/differentiation factors (10 ng/ml each ...
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bioRxiv - Neuroscience 2020Quote: ... or under glycerol:TBS (9:1) with Hoechst 33342 (2.5µg/ml, ThermoFisher Scientific). Primary antibodies used in the study were rabbit anti-tyrosine hydroxylase (1:1000 ...
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bioRxiv - Biochemistry 2020Quote: ... The fluorogenic probe 7-diethylamino-3-(4’-maleimidylphenyl)-4-methylcoumarin (abbr. CPM, ThermoFisher, Cat# D346) in 100% DMSO was then added to both quench the enzymatic reaction and react with CoASH to yield the fluorescent CPM-SCoA complex for fluorescence quantification 27 ...
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bioRxiv - Biochemistry 2023Quote: ... 150 mM NaCl pH 9 using a Slide-A-Lyzer 0.5 - 3 mL 10,000 MWCO dialysis cassette (Thermo Scientific). The conjugation reaction was performed overnight at 21 °C and 750 rpm with 2 equivalents of DBCO-SL8 or DBCO-PEG4-SL8 per STxB monomer and 10 % DMSO to keep the peptides soluble ...
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bioRxiv - Biochemistry 2023Quote: ... NaHCO3 and 150 μL acetone containing 4 mg/mL 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide (L-FDAA, Thermo Scientific) was added ...
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bioRxiv - Immunology 2021Quote: ... 48 hours after transfection cells were splitted in a ratio of 1:4 and 300 μg/ml Hygromycin B (Thermo Fisher Scientific) was added to the cell culture medium for antibiotic selection of stably transfected cells ...
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bioRxiv - Neuroscience 2024Quote: ... The passage was performed twice per week (1:2 or 1:3) using Accutase (Cat #A1110501, Thermo Fisher Scientific) by vigorously breaking pellets to remove neuronal cells ...
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bioRxiv - Cancer Biology 2021Quote: ... 2% B-27 supplement w/o vitamin A (v/v) (Thermo Fisher #12587010), 20 ng/ml H-EGF (Shenandoah #100-26) ...
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bioRxiv - Biochemistry 2020Quote: ... containing 2% (v/v) B-27 Supplement (Cat#17504044, Gibco/Thermo Fisher Scientific), Glutamax I (4 mM ...
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bioRxiv - Biochemistry 2020Quote: ... containing 2% (v/v) B-27 Supplement (Cat#17504044, Gibco/Thermo Fisher Scientific), Glutamax I (4 mM ...
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bioRxiv - Genetics 2022Quote: ... 2% B-27 supplement without retinoic acid (Fisher Scientific, Cat #: 12-587-010), and 1% antibiotic/antimycotic (Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... 2% (v/v) B-27 supplement (catalog number 17504044, Gibco / Thermo Fisher Scientific), 1% (v/v ...
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bioRxiv - Neuroscience 2022Quote: ... 2% (v/v) B-27 supplement (catalog number 17504044, Gibco / Thermo Fisher Scientific), 1% (v/v ...
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bioRxiv - Bioengineering 2023Quote: ... cells were maintained in RPMI 1640 medium with B-27 (2%) (Gibco, #17504044) (CM(+) ...
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bioRxiv - Cell Biology 2023Quote: ... Dissociated neurons were resuspended in Neurobasal-A medium containing 2% B-27 (Gibco) and 0.25% GlutaMAX supplements (Gibco) ...
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bioRxiv - Bioengineering 2023Quote: ... cells were maintained in RPMI 1640 medium with B-27 (2%) (Gibco, #17504044) (CM(+) ...
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bioRxiv - Neuroscience 2023Quote: ... 1X N-2 (Thermo Fisher Scientific, 17502-048], 1X B-27 [Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... rat anti-B-lymphocyte activation antigen B7-2 (CD86, Invitrogen, MA1-10299, RRID:AB_11152324), guinea pig anti-vesicular glutamate transporter 1 (vGlut1 ...
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bioRxiv - Cell Biology 2024Quote: ... The electroporated cells were plated in one well of 6 well plate with StemFlex media (ThermoFisher Scientific) supplemented CloneR (Stemcell technologies) ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4’,6-diamidino-2-phenylindole (DAPI) (00-4959-52, Invitrogen, Thermo Fisher Scientific, MA, USA) as counterstaining.
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bioRxiv - Bioengineering 2020Quote: ... the coverslips were placed upside down on a glass slide with slow fade mounting medium containing 4’,6-diamidino-2- phenylindole (DAPI, Life Technologies, Carlsbad, CA, USA) and kept at 4 °C until observation ...
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bioRxiv - Microbiology 2021Quote: ... Nuclei were counterstained with Hoechst 33342 DNA dye NucBlue® Live ReadyProbes® reagent for live cell imaging or 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI) and SYTO® 60 fluorescent nucleic acid stain for fixed samples (Invitrogen, Molecular Probes, Germany).
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bioRxiv - Biochemistry 2020Quote: ... Coverslips were mounted and cells were counterstained on the glass slides using ProLong Gold with DAPI (4’, 6-diamidino-2-phenylindole) (Molecular Probes, Eugene, OR, US). Images were collected using an Axio Plan2/AxioCam microscope and image processing was performed with MrC5/Axiovision software (Zeiss ...
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bioRxiv - Immunology 2021Quote: ... bacteria were stained with 2µg/µL of 4’,6-diamidino-2-phenylindole (DAPI) and mounted in ProLong Diamond Antifade (Life Technologies, Eugene, OR, USA; P36961). Image acquisition was performed using a Leica confocal microscope (Leica ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were stained with DAPI (4’,6-diamidino-2-phenylindole) and cover-slipped with ProLong Glass Antifade Mountant (P36984, Thermo Scientific, Waltham MA, USA). All images were acquired on a Keyence-BZ800 microscope ...
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bioRxiv - Microbiology 2021Quote: ... Cells infected with YFV were incubated with the pan-flavivirus anti-Env 4G2 antibody for 1h at 4°C and then with Alexa 488 anti-mouse IgG secondary antibodies (Thermo Fisher, #A28175) for 45 min at 4°C in the dark ...
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bioRxiv - Neuroscience 2020Quote: ... for 1h at room temperature (or overnight at 4°C) and the unbound dye was removed using spin columns (Thermofisher, Waltham, MA). The labeling efficiency was estimated using absorbance values (488 or 555 or 660 nm/280 nm ...
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bioRxiv - Neuroscience 2022Quote: ... Protein lysates were pre-cleared 1h at 4°C on a rotating wheel with 50μl of previously washed Dynabeads Protein G (Invitrogen, Thermo Fisher Scientific), to avoid aspecific bindings ...
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bioRxiv - Plant Biology 2022Quote: ... from cauline leaves of flowering plants by measuring the quantity of 4-methylumbelliferone product generated from the substrate 4-methylumbelliferyl-b-D-glucuronide (Duchefa) on a fluorometer (Fluoroscan II; Thermo Scientific).
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bioRxiv - Genomics 2021Quote: ... The embryoid medium was aspirated and 15 to 20 embryoid bodies were plated per well in 6-well plates and cultured in 3 mL hematopoetic medium (2 mM GlutaMax, 1x Antibiotic-Antimycotic (Thermo Fisher Scientific; Cat. No. 15240062), 55 mM 2-mercaptoethanol (BioRad ...
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bioRxiv - Molecular Biology 2020Quote: ... Detection of SARS-CoV-2 N1 gene sequences and host RNase P mRNA from de-identified SARS-CoV-2 positive samples was carried out with the one-step TaqMan RNA-to-Ct 1-Step Kit (ThermoFisher), using 2 μL of undiluted PEARL extract ...
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bioRxiv - Biochemistry 2020Quote: ... one µg of small RNAs was mixed with 2 µg of HPDP-Biotin (ThermoFisher Scientific, 1 mg/ml in DMSO). The reaction was incubated for three hours at room temperature protected from light and mixed every 15 minutes ...
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bioRxiv - Genetics 2019Quote: ... BbsI-linearized piggyBac-U6 backbone was mixed with purified PCE product in a vector:insert 2:1 ratio and was directly used for transformation of One Shot MAX Efficiency DH5α-T1 Competent Cells (Invitrogen).