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Citations for Thermo Fisher :
1801 - 1850 of 10000+ citations for 6 Methoxy 2 3 4 9 tetrahydro 1H b carbolin 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... NaV1.4 (GenBank: MZ545381.1) expressed in a pCDNA3.1 vector (9) was made using the mMACHINE™ T7 Transcription Kit (Invitrogen). Xenopus oocytes were injected with 3– 6 ng of Pt NaV1.4 and TEVC experiments were performed 1–2 days post-injection ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated overnight in mouse anti-Hu primary antibody at 4°C (1:200 in 3% block; Invitrogen). After three 5-min PB rinses at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... 4) counterstained with the nuclear dye TO-PRO-3 (diluted 1:10’000; Life Technologies, Inc., Gaithersburg, MD, USA). Finally ...
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bioRxiv - Microbiology 2021Quote: ... Paris) and pCMV-VSV-G at a ratio of 4:3:1 with Lipofectamine 3,000 (Thermo Fisher Scientific). Supernatants were collected 48 h after transfection ...
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bioRxiv - Physiology 2022Quote: ... The samples were combined with 1 ml of 3:13 dilution of Erlich’s reagent [1.5 g of 4- (dimethylamino) benzaldehyde (ThermoFisher); 5 ml ethanol ...
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bioRxiv - Immunology 2021Quote: ... Cells were maintained at 3×105 to 9×105 cells/ml in Roswell Park Memorial Institute medium (RPMI GlutaMAX, Gibco) supplemented with 10% (vol/vol ...
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bioRxiv - Microbiology 2020Quote: ... Israel) were maintained at 3×105 to 9×105 cells/ml in Roswell Park Memorial Institute medium (RPMI GlutaMAX, Gibco) supplemented with 10% (vol/vol ...
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bioRxiv - Immunology 2020Quote: ... TIB-47) were maintained at 3×105 to 9×105 cells/ml in Roswell Park Memorial Institute medium (RPMI GlutaMAX, Gibco) supplemented with 10% (vol/vol ...
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bioRxiv - Synthetic Biology 2020Quote: ... transfection complexes containing 3 μL of Xtremegene-9 reagent and 500 ng of plasmid DNA were prepared in 100 μL of OptiMEM (Invitrogen). After 20 min of incubation at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... A 11.2 kb PCR product that included a region from CLN3 exon 3 to exon 9 was amplified using a high fidelity polymerase (Platinum Taq High Fidelity, Invitrogen) and CLN3 primers pCLN3F3 and pCLN3R3 (Supplemental Table 2) ...
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bioRxiv - Microbiology 2024Quote: ... Israel) were maintained at 3×105 to 9×105 cells/ml in Roswell Park Memorial Institute medium (RPMI GlutaMAX, Gibco) supplemented with 10% (vol/vol ...
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bioRxiv - Biochemistry 2023Quote: ... Cell proliferation was assessed at different time points (3, 5, 7 and 9 days) using AlamarBlue Cell Viability Reagent (Invitrogen) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Previously pelleted spheroplasts were resuspended in 1 mL 10mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer solution (Gibco) and 100 μl were collected as whole spheroplasts ...
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bioRxiv - Neuroscience 2023Quote: ... p21) and pAAV target plasmid in a 4:1:1:2 molar ratio by use of Lipofectamine 2000 (ThermoFisher) according to company protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged with 80-90% confluence at a split ratio of 1:2-1:4 using TrypLE (Gibco) for 8 min at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... Cells nuclei were counter-stained using 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI) mounting medium (ProLong™ Diamond Antifade Mountant with DAPI, Thermo Fisher Scientific, Warsaw, Poland). Preparations were observed using the confocal microscope under magnification 630× and processed with Fiji software (ImageJ 1.52n ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4’,6-diamidino-2-phenylindole (DAPI) (00-4959-52, Invitrogen, Thermo Fisher Scientific, MA, USA) as counterstaining.
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bioRxiv - Molecular Biology 2022Quote: A concentration of 6 × 103 cells was loaded with 5 mM 4-amino-5-methylamino-2’,7’- difluorofluorescein diacetate (DAF-FM, Molecular Probes, Thermo Fisher, Sao Paulo, Brazil) after 72 h of treatment ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated for 1h with secondary antibodies (Invitrogen). Afterwards ...
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bioRxiv - Neuroscience 2022Quote: ... or 1h with 10% normal goat serum (Gibco BRL) and 0,4% Triton X-100 (for PLP) ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1:1 Supplement B-27 Minus Vitamine A (Gibco,12587-010), filtered with a 0.45 μm filter (VWR ...
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bioRxiv - Cell Biology 2024Quote: ... 100 μg.mL-1 streptomycin and 1.25 μg.mL-1 amphotericin B (Life Technologies) and maintained in culture at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 3 kb RT-PCR products covering the S gene deletion were amplified from the viral RNA using the gene specific primers F9newF and F9newR (5’-TAAGGTTGGTGGTAATTATAATTACCTG-3’ and 5’-AAAATAGTTGGCATCATAAAGTAATGGG-3’) and a SuperScript™ IV One-Step RT-PCR System (Invitrogen™, ThemoFisher). A region spanning the deletion was sequenced using primers Wu_24_L and Wu_24_R (5’-TTGAACTTCTACATGCACCAGC-3’ and 5’-CCAGAAGTGATTGTACCCGC-3’).
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bioRxiv - Cell Biology 2021Quote: ... Cell pellets were then resuspended and plated into one well of a 6 well plate (Fisher Scientific 1483211). Cells were left to sit for 5 days at 37°C and 5% CO2 after initial plating and were only fed once after three days by adding an additional volume of FCM ...
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bioRxiv - Neuroscience 2023Quote: ... EBs were seeded on one well of a 6-well plate coated with poly-Ornithine/Laminin (Thermo Scientific). Medium was changed every other day ...
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bioRxiv - Plant Biology 2022Quote: ... and 2 mL of 1:10 diluted cDNA in an Applied Biosystems Step One Plus real-time PCR instrument (ThermoFisher, USA), according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... Then 1 mL dye solution was mixed with 9 grams glycerol (15514011, Invitrogen) and added 400 μl solution to a home-made sample holder for 3D-SMART experiments (Supplementary Fig ...
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bioRxiv - Cell Biology 2022Quote: ... were transfected with 1-9 µg of bacmid DNA using ExpiFectamine (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... mounted with fluorescence mounting medium (9 ml of glycerol [BP229-1; Fisher Scientific] ...
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bioRxiv - Cancer Biology 2023Quote: ... mounted with fluorescence mounting medium (9 ml of glycerol [BP229-1; Fisher Scientific] ...
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bioRxiv - Cancer Biology 2023Quote: ... mounted with fluorescence mounting medium (9 ml of glycerol [BP229-1; Fisher Scientific] ...
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bioRxiv - Cancer Biology 2019Quote: ... All TaqMan probes were 5′-6-carboxyfluorescein (FAM) and 3′-6-carboxy-N,N,N′,N′-tetramethylrhodamine (TAMRA) labeled (Applied Biosystems, US) except TATA-binding protein (TBP ...
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bioRxiv - Neuroscience 2021Quote: ... fluorescently conjugated Cholera toxin subunit B (CTB-555, 2 μg/μl, 300 nl, Invitrogen) was injected unilaterally into VLS ...
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bioRxiv - Neuroscience 2021Quote: ... in Neurobasal medium enhanced with 2% B-27 and 0.5% L-Glutamine (Life technologies). Primary hippocampal neurons growing on either 12mm or 18mm coverslips ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with B-27 and 2 mM Glutamax (Gibco; Thermo Fisher, Waltham, MA, USA). Primary neuronal cultures were infected with AAV-ESARE-mScarlet-Flag particles at a titer of 4.35×106 genome copies/mL and AAV-CaMK0.4-FHS-paCaMKII-WPRE3 at a titer of 4.72×106 genome copies/ml at DIV 9 ...
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bioRxiv - Neuroscience 2020Quote: Fluorescently conjugated Cholera toxin subunit B (CTB-555, 2 μg/μl, 300 nl, Invitrogen) was injected unilaterally into V1 (AP ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with B-27 and 2 mM Glutamax (Gibco; Thermo Fisher, Waltham, MA, USA). Primary neuronal cultures were infected with AAV-ESARE-mScarlet-Flag particles at a titer of 4.35×106 genome copies/mL and AAV-CaMK0.4-FHS-paCaMKII-WPRE3 at a titer of 4.72×106 genome copies/ml at DIV 9 ...
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bioRxiv - Genomics 2020Quote: ... 2 µl cDNA (diluted 1:20) was used in a 6 µl Fast SYBR Green qPCR reaction (ThermoFisher Scientific). No-template controls for each gene were run in all qPCR plates ...
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bioRxiv - Biochemistry 2019Quote: ... fluorescent probes Prodan (6-propionyl-2[dimethylamino]-naphthalene) and ANS (1-anilinonaphthalene-8-sulfonic acid) were also from Invitrogen; PMB ...
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bioRxiv - Biophysics 2024Quote: ... at a 6:1:2 ratio for each virus using the Bac-to-Bac baculovirus expression system (Thermo Fisher). Cell cultures were grown in ESF 921 medium (Expression Systems ...
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bioRxiv - Biophysics 2024Quote: ... and 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydro (EDC, ThermoFisher). For BS3 crosslinking ...
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bioRxiv - Immunology 2022Quote: Caspase-3 activity was determined using EnzChek™ Caspase-3 Assay Kit #2 (Thermo Fisher).
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bioRxiv - Microbiology 2021Quote: ... (75 mm i.d. 3 2 cm, Acclaim PepMap100 C18 3 mm, 100 A°, ThermoFisher Scientific) and separated over an EASY-Spray column ...
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bioRxiv - Cell Biology 2022Quote: ... DNP (EM 1:6-1:30, #71-3500, Invitrogen), DPP4 (IF/FACS 1:100 ...
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bioRxiv - Developmental Biology 2021Quote: ... 2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]- D-glucose (2-NBDG) (Invitrogen N13195) was used as a probe for monitoring glucose uptake ...
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bioRxiv - Cell Biology 2020Quote: ... or BODIPY™ 558/568 C12 (4,4-Difluoro-5-(2-Thienyl)-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Neuroscience 2021Quote: ... Samples were incubated at 4°C for 3 hours on a rotator before being placed in a DynaMag™-2 magnetic rack (Thermo Scientific, 12321D) and washed twice with TBS+NP40 wash buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Fluorescence-labelled 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (NBD-PE) was obtained from Molecular Probes (Eugene, Oregon, US). Sucrose was purchased from XiLong Chemical Co. ...
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bioRxiv - Cancer Biology 2022Quote: ... 2-4 μg/ml of Blastidin (ThermoFisher Scientific) and 100-400 μg/ml of G418 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Puromycin (2-4 μg/ml, Thermo Fisher, A113803) was used for TRIM25-knockdown HEK293T cell selection ...