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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: Cells employed in the studies are HEK293 (Gibco 293-H cells), GM08207 (Coriell Cell Repositories ...
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bioRxiv - Biochemistry 2023Quote: Expi293F is a derivative of the HEK293 cell line (Thermo Fisher). Expi293F cells were grown in Expi293 Expression Medium (Thermo Fisher) ...
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bioRxiv - Biophysics 2023Quote: ... HEK293S GnTI-cells (ATCC) were cultured in Freestyle 293 medium (GIBCO) supplemented with 2% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... The siRNAs were transfected into HEK293 cells using Lipofectamine 2000 (Invitrogen). The effect of knockdown was validated by western blot.
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 FRT cells were cultured in zeocin (100 μg/ml, Invitrogen) to maintain the integrated FRT site ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells (ATCC) were cultured in Dulbecco’s Modified Eagles Medium (Gibco) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Biochemistry 2023Quote: HEK293-F cells were grown in FreeStyle 293 Expression Medium (Gibco) at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells were grown in Dulbecco’s modified Eagle medium (DMEM; Gibco) containing 10% (v/v ...
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bioRxiv - Physiology 2023Quote: HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco, France) supplemented with 10% fetal calf serum (Eurobio ...
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bioRxiv - Bioengineering 2023Quote: ... Human Embryonic Kidney cells (HEK293-CD19+) were cultured in DMEM (Gibco), 10% heat-inactivated FBS (Gibco) ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293-F cells were grown in FreeStyle 293 expression medium (Gibco) (20 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... HEK293 cells were washed with Opti-MEM Reduced Serum Media (Gibco) and incubated with transfection mix containing 1 µg total of the indicated plasmid DNA and 3 µL Lipofectamine 3000 Transfection Reagent (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... After transfection the Flp-In TRex HEK293 (Flp-In-293, Invitrogen) cells were selected in the presence of Hygromycin B (100 µg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 and C166 cells were maintained in DMEM (high glucose, Gibco) supplemented with 10% FBS (Giboco) ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293-FT (RRID:CVCL_6911) were culture similarly but in DMEM medium (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... HEK293 cells were washed with Opti-MEM Reduced Serum Media (Gibco) and transfected using lipofectamine 2000 (Invitrogen/Life Technologies Cat #11668019 ...
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bioRxiv - Molecular Biology 2024Quote: HEK293-T cells were cultured in DMEM medium (#31966-021, Gibco) supplemented with 10% fetal bovine serum (#10270-106 ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells were grown in Dulbecco’s Modified Eagle Medium (DMEM, ThermoFisher) containing 10% fetal calf serum and 0.5% penicillin/streptomycin ...
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bioRxiv - Cancer Biology 2022Quote: ... and rotated at 4°C overnight with 2-5 ug of antibody (H3K27ac, Active Motif Cat #39133; V5 tag, ThermoFisher #R960-25 ...
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bioRxiv - Biochemistry 2023Quote: ... A total of 2 mg of lysate per condition together with 25 μL of DynabeadsTM for His-Tag isolation (Invitrogen) were incubated with His6-AtLEGβ or the His6-proAtLEGβ-C211A dead mutant or lysate alone for an hour at room temperature with rotation ...
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bioRxiv - Biochemistry 2020Quote: ... one µg of small RNAs was mixed with 2 µg of HPDP-Biotin (ThermoFisher Scientific, 1 mg/ml in DMSO). The reaction was incubated for three hours at room temperature protected from light and mixed every 15 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were then reacted for 2 h at RT with the following click reagents: 50 μM biotin-alkyne (ThermoFisher Scientific), 5 mM tris(3-hydroxypropyltriazolylmethyl)amine (THPTA ...
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bioRxiv - Biochemistry 2022Quote: ... 550 pmol of protein was then added to a tube containing 1.1 nmol of 5’Biotin(GGTTAG)2 DNA (Thermo Fisher) in a volume of 100 µL dilution buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended in 300 μl of Binding Buffer with 2 μg of biotin-labeled tubulin peptide (K40 only, K40-Ac and K40-me3) (ThermoFisher), and samples were rotated at 4 °C for 2 h ...
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bioRxiv - Neuroscience 2022Quote: ... Biotinylated proteins were eluted in elution buffer (20 µL of 20 mM DTT and 2 mM Biotin in 1X NuPAGE LDS lysis buffer (ThermoFisher) with protease inhibitor and phosphatase inhibitor ...
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bioRxiv - Molecular Biology 2023Quote: ... γ-TuRC was biotinylated prior to elution by incubation for 1 hour on ice in CSF-xB 2% (w/w) sucrose supplemented with 40 μM NHS-PEG4-biotin (ThermoFisher). The elution was concentrated using a 100 kDa MWCO spin concentrator (Amicon) ...
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bioRxiv - Microbiology 2020Quote: ... EVs were resuspended in D-MEM (0 % FCS) + fluorescent Bodipy Phosphocholine analogue at 2 μg/mL (D3793, Thermofisher Scientific) and incubated overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Extracellular antibodies were diluted in FACS buffer (2% FBS and 5mM EDTA in PBS) with Fc Block (Life Technologies) and incubated for 30 minutes at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... These cells were washed with PBS and then resuspended in PBS supplemented with 2% FCS or FBS and 5µM CM-H2DCFDA probe (Invitrogen) and incubated in the dark for 20min at 37°C under 5% CO2 ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissues were immersed in 2ml dissociation solution (2% FCS-PBS solution with approximate 145U/ml type 4 Gibco collagenase) and incubated at 37°C for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... in R10 media (RPMI 1640 with 10% FCS, 1% penicillin/ streptomycin, and 2 mM L-glutamine, Thermo Fisher Scientific) at 37°C in 5% CO2 to differentiate quiescent MBCs into mASCs ...
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bioRxiv - Immunology 2021Quote: ... The hACE-2-Fc content in the supernatant was quantified by ELISA using 96-Well MaxiSorp Immuno plates (ThermoFisher) coated overnight with 200 ng/well of RBD219-WT protein ...
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bioRxiv - Immunology 2023Quote: ... or IL-2 combined with spike protein for 16 hrs and used for RNA extraction using TRIzol™ reagent (Thermo Fisher Scientific, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Desalted peptides were labeled with Tandem Mass Tags (TMT) using TMTPro-16plex isobaric tags (ThermoFisher Scientific; #A44520) as per manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant AtCSD1 protein containing a 6xHis-tag was purified using Dynabeads His-Tag (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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Variation of wine preference amongst consumers is influenced by the composition of salivary proteinsbioRxiv - Biochemistry 2023Quote: Tandem mass tag (TMT) labelling was performed using the TMT10plexTM Mass Tag Labelling Kit (Thermo Fisher Scientific) with minor modifications from the manufacturer’s protocol ...
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bioRxiv - Pathology 2021Quote: ... we used the SARS-CoV2 nucleocapsid (Invitrogen #MA-17404) and spike protein (Sino Biological #40150-T62 ...
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bioRxiv - Cell Biology 2021Quote: ... The fraction of biotin-labeled tubulin was measured using a Biocytin Biotin Quantification Kit (Thermo Scientific #44610). Final stocks were diluted using unlabeled tubulin to 40 μM with a 33% biotin-labeled fraction.
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bioRxiv - Microbiology 2022Quote: ... and the resulting 160-bp amplicon biotin-labeled using the Biotin 3’ End DNA Labeling kit (ThermoFisher). EMSA reactions were prepared in 20 µl reaction mixtures in binding buffer (10 mM Tris ...
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bioRxiv - Neuroscience 2022Quote: ... BCA protein assay reagents and sulfo-NHS-LC-biotin and sulfo-NHS-SS-biotin from Thermo Fisher Scientific Inc. ...
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bioRxiv - Molecular Biology 2022Quote: ... HT-DNA and scDNAs were biotin-labeled using EZ-Link Hydrazide Biotin (#21339, Thermo Fisher Scientific Inc.), EDC (#22980 ...
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bioRxiv - Biochemistry 2023Quote: ... tRNAGln was 3’ biotin labeled with the Pierce ™ RNA 3’ end biotin labeling kit (Thermo Fisher) according to manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2020Quote: ... staining by sheep anti-DIG antibody (PA1-85378, 1:200, Life Technologies) and mounting were the same as described above for immunofluorescence staining ...
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bioRxiv - Cell Biology 2020Quote: ... Dynabeads sheep anti-rat IgG was purchased from Invitrogen (Carlsbad, CA, USA). VectaMount permanent mounting medium was used from Vector Laboratories (Burlingame ...
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bioRxiv - Microbiology 2019Quote: ... The sheep blood agar (SBA) was purchased from Thermo Scientific (Waltham, MA).
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bioRxiv - Bioengineering 2020Quote: ... and donkey anti sheep 568 goat anti-rat secondary antibody (Thermo Fisher). The secondary antibodies were removed ...
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bioRxiv - Physiology 2021Quote: ... followed by secondary rabbit anti-sheep antibodies (Thermo Fisher Scientific, Waltham, MA) and ImmPRESS polymer anti-rabbit IgG reagent (Vector Laboratories ...
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bioRxiv - Microbiology 2020Quote: Hemolysis assay using red blood cells (defibrinated sheep blood, Thermo Fisher Scientific) was performed as previously described40.
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bioRxiv - Cell Biology 2020Quote: ... and Alexa Fluor 555 anti-sheep IgG (A11015; Life Technologies; 1:500) secondary antibody ...
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bioRxiv - Molecular Biology 2022Quote: ... Supplementary Table 4) on Sheep Anti-Mouse IgG Dynabeads M-280 (ThermoFisher) overnight at 4°C ...