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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for Respiratory syncytial virus RSV fusion protein exodomain human heterodimeric IgG1 Fc tag recombinant antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... a plasmid encoding the EGFP-N fusion protein was constructed and transfected into infected HEp-2 cells with Lipo3000 (Thermo Fisher, USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... Constructs for CRK2–YFP and YFP–Myc fusion proteins were created using the MultiSite Gateway technology (Invitrogen; Thermo Fisher Scientific, Waltham, USA). The coding sequence of CRK2 was amplified by PCR (forward primer ...
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bioRxiv - Bioengineering 2020Quote: The IGFBP-derived NLS peptides (NLS-3 and NLS-5) were extracted from the bacteria using the B-Per 6xHis Fusion Protein Purification Kit (Thermo Scientific, USA), according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... pastoris was confirmed by detecting CSGA-GFP fusion protein using polyclonal rabbit anti-GFP antibodies (from Life Technologies USA; cat # A-11122). Amount of protein was normalized ...
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bioRxiv - Cell Biology 2022Quote: N2A cells were transfected as described above with BicD2-KIF tail fusions and MBNL-GFP proteins at a ratio of 2:3 in 4-well chamber slides (Thermo Fisher, 154526). 24 hours after transfection ...
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bioRxiv - Genomics 2024Quote: ... The resulting 6x-MBP-hBPTF (701-1073) fusion protein was expressed in the Escherichia coli host strain BL21(DE3) (Thermo Fisher Scientific), and the soluble protein was purified on a HisTrap FF column (Cytiva) ...
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bioRxiv - Biochemistry 2022Quote: The UV-Vis spectra of E7-Fd and E7-Fd-FNR fusion proteins were measured using a UV-2450 spectrophotometer (Shimadzu) and NanoDrop™ 2000 spectrophotometer (Thermofisher ™), respectively ...
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bioRxiv - Cell Biology 2023Quote: The SUMO-CCM2(413-438) contains a single cysteine (C437) that was used to couple the fusion protein with a maleimide-fluorescein dye (Thermo Fisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... All redesigned RSV-F and hMPV-F constructs were subcloned into a CMV/R vector and transiently expressed in ExpiCHO cells (Thermo Fisher, catalog no. A29133) as previously reported (69 ...
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bioRxiv - Immunology 2021Quote: ... and searched against the UniProt Human protein database and RefSeq SARS-CoV-2 protein database using Sequest HT through Proteome Discoverer (Version 2.2) (Thermo Scientific, Bremen, Germany). Precursor and fragment mass tolerance were set to 10 ppm and 0.02 Da ...
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bioRxiv - Molecular Biology 2020Quote: ... connected to a Orbitrap Fusion (Thermo Scientific). The data were acquired using 120000 resolution for the peptide measurements in the Orbitrap and a top T (3s ...
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bioRxiv - Neuroscience 2019Quote: ... and Orbitrap Fusion Lumos (Thermo Fisher Scientific) coupled to an Agilent 1290 HPLC system (Agilent Technologies) ...
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bioRxiv - Molecular Biology 2020Quote: ... The mass spectrometer (Fusion Lumos, Thermo Fisher) was operated in High/High mode with MS and MS/MS mass resolution being 120,000 and 30,000 ...
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bioRxiv - Biochemistry 2020Quote: ... a Thermo Orbitrap Fusion (Thermo Fisher Scientific) was operated in polarity switching mode and the MS settings were as follows ...
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bioRxiv - Cell Biology 2021Quote: ... An Orbitrap Fusion mass spectrometer (Thermo Scientific) analyzed the eluted peptides that were ionized in a nano-Easy electrospray source ...
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bioRxiv - Molecular Biology 2022Quote: ... connected to an Orbitrap Fusion (Thermo Scientific) at 150 nl/min flow rate ...
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bioRxiv - Cell Biology 2021Quote: ... coupled to an Orbitrap Fusion (ThermoFisher Scientific) tandem mass spectrometer ...
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bioRxiv - Neuroscience 2022Quote: ... A coupled Orbitrap Fusion (Thermo Fisher Scientific) was used to generate mass spectrometry (MS ...
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bioRxiv - Developmental Biology 2022Quote: ... The Orbitrap Fusion instrument (Thermo Fisher Scientific) was run in an SPS MS3 top speed method with FAIMSPro ion mobility enabled (2 CVs ...
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bioRxiv - Cancer Biology 2022Quote: ... Orbitrap Fusion Lumos™ Tribrid (Thermo Scientific) mass spectrometer was operated in data-dependent acquisition (DDA ...
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bioRxiv - Molecular Biology 2024Quote: Instrument: Orbitrap Fusion LUMOS (Thermo Fisher Scientific) with VanquishNeo-nLC and an easy source with a 75um×15cm EasyC18 column ...
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bioRxiv - Molecular Biology 2024Quote: ... connected to an Orbitrap Fusion (Thermo Scientific) at 150 uL/min flow rate ...
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bioRxiv - Cancer Biology 2024Quote: ... An Orbitrap Fusion mass spectrometer (Thermo Scientific) was operated in data-dependent acquisition mode with the following parameters ...
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bioRxiv - Cell Biology 2023Quote: ... and Orbitrap Fusion Lumos (Thermo Fisher Scientific) were used as a LC-MS/MS system ...
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bioRxiv - Molecular Biology 2021Quote: ... fluorescence-labeled HIV-1JR-FL Env(-) carrying the (His)6 epitope tag was incubated with biotin-conjugated anti-(His)6 tag antibody (HIS.H8, Invitrogen) at 4° for two hours.
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bioRxiv - Immunology 2023Quote: ... Fc receptors were blocked by incubating cells with 1:50 anti- mouse CD16/CD32 (Fc block, Invitrogen) for 10 minutes at 4°C ...
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bioRxiv - Immunology 2023Quote: ... and H1N1/2009 influenza virus strains were prepared by overnight incubation of the virus strains with 0.1% v/v β propiolactone (Acros Organics, Geel, Belgium) under continuous rotation at 4°C ...
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bioRxiv - Immunology 2022Quote: All experiments were performed at 4°C using ProtoArray Human Protein Microarrays (Thermo Fisher Scientific). Microarrays were blocked for 1 h in blocking solution (Thermo Fisher) ...
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bioRxiv - Microbiology 2020Quote: ... HK-2 cells were maintained in keratinocyte serum-free (KSF) media with 0.05 mg/mL bovine pituitary extract and 5 ng/mL human recombinant epidermal growth factor (Gibco - Thermo Fisher Scientific, MA, USA). All culture conditions contained a 5% antibiotic / antimycotic mixed solution (Nacalai Tesque) ...
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bioRxiv - Cancer Biology 2020Quote: ... Recombinant human IFNγ (285-IF) was purchased from R & D Systems (Minneapolis, MN) and mouse IFNγ (BMS326) from eBioscience (Thermo Fisher Scientific, Waltham, MA). The following antibodies were used for Western blot ...
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bioRxiv - Cancer Biology 2024Quote: ... PWR-1E and PIN cells were cultured in Keratinocyte Serum-Free Medium supplemented with bovine pituitary extract and recombinant human epidermal growth factor (Gibco, Life Technologies, Grand Island, NY). Myc-CaP ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% fetal bovine serum except for RWPE-1 and RWPE-2 cell lines which were grown in a keratinocyte serum-free medium supplemented with 5 ng/mL recombinant human epidermal growth factor (EGF) and 50 μg/mL bovine pituitary extract (Gibco™, Invitrogen, Carlsbad, CA, USA). The cell lines were tested for short tandem repeat (STR ...
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bioRxiv - Microbiology 2024Quote: ... and cultured in Gibco® keratinocyte serum-free medium (K-SFM) supplemented with recombinant human EGF and bovine pituitary extract (Thermo Fisher Scientific, #17005075). The cells were propagated in 75cm2 flasks (Falcon ...
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bioRxiv - Bioengineering 2019Quote: ... was diluted 1:200 in PBS/5% FCS/0.1% Tween® 20 and primary MMP1 antibody (mouse anti-human; Invitrogen Thermo Fisher Scientific, monoclonal, MA5-15872) was diluted 1:500 in PBS/5% FCS/0.1% Tween® 20 and incubated according to the manufacturer’s instruction for 3 h ...
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bioRxiv - Molecular Biology 2021Quote: ... designed using CRISPOR) and 7.5 μg recombinant Cas9 protein (Vienna Biocenter Core Facilities) using the Neon Transfection System (Thermo Fisher Scientific). A week later ...
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bioRxiv - Microbiology 2020Quote: One 96-well plate was coated with 200ng/well of recombinant N protein and another plate was coated with 200ng/well of recombinant 6xHis-tagged SARS-CoV-2 spike protein receptor binding domain (RBD) produced using the FreeStyle 293 Expression System (Thermo Fisher) from BEI Resources (Cat# NR-52366 ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was added to 15 mL of glutathione agarose resin (which binds up to 40 mg of purified recombinant GST protein per milliliter of resin) (Thermo Scientific) and incubated on ice (4°C ...
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bioRxiv - Microbiology 2020Quote: ... recombinant SARS-CoV-2 spike protein was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Immunology 2021Quote: Monoclonal antibodies or recombinant SARS-CoV-2 RBD + SBD1 or NTD proteins were transfected in FreeStyle 293F suspension cells (Life Technologies) using PEIMAX transfection reagent (Polysciences) ...
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bioRxiv - Immunology 2021Quote: Thermal melt analysis of the recombinant proteins was performed in triplicate in 96-well plates in a QuantStudio 7 Flex real time PCR machine (Applied Biosystems). Each well contained 2-4 μg protein in buffer (25mM TRIS pH 8 ...
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bioRxiv - Molecular Biology 2022Quote: ... Then 3 μg of purified recombinant protein was added with 4 μl of RiboLock RNase Inhibitor (Thermo Fisher Scientific, Waltham, MA). The mixture was incubated at room temperature for 1 hour ...
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bioRxiv - Developmental Biology 2024Quote: ... 293 cells were transfected with plasmids encoding His-tagged recombinant proteins as described in the Plasmids section using lipofectamine 2000 (Invitrogen, #11668019) following the manufacturer’s instructions (four 100 mm plates each plasmid) ...
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bioRxiv - Microbiology 2024Quote: ... Purity of the recombinant PfPKG proteins was assessed by SDS-PAGE using NuPAGETM Novex® 3-8 % Tris-Acetate gels (Invitrogen) and expression was confirmed by Western blot analysis.
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 Spike RBD 319-541 recombinant protein (OBA0101, Ovodan Biotech) was expressed in 25 mL culture using the Expi293F expression system (#A14635; ThermoFisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The cells were treated with the appropriate concentration of recombinant proteins and YO-PRO (Thermo Fisher Scientific, diluted at 1:2000) and incubated at 37°C ...
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bioRxiv - Immunology 2023Quote: ... designed using CRISPOR) and 7.5 μg recombinant Cas9 protein (Vienna Biocenter Core Facilities) using the Neon Transfection System (Thermo Fisher Scientific). A week later ...
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bioRxiv - Biochemistry 2023Quote: Recombinant trimeric spike proteins (5 µg/mL) were plated in 50 µl in each well on a MaxiSorp (Thermo Fisher Scientific) microtiter plate in 1xPBS and left to incubate for at least 1 h at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: About 3 ml of bleed 3 was diluted in 7 ml of sterile 1X PBS and passed through 0.2 μM filter and incubated with recombinant protein G sepharose 4B beads (Life Technologies 101242) at 4℃ overnight ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then transfected overnight with 100 pmol of tracrRNA and crRNA complexed with 4 pmol recombinant cas9 protein using RNAiMAX (Thermo Fisher). Medium was then replaced and cells were grown for 48 h before assessing fluorescence and sorting.
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bioRxiv - Biophysics 2021Quote: ... a fluorophore-conjugated primary antibody (mouse ZO1-1A12 IgG1 AlexaFluor 488; Invitrogen, ThermoFisher Scientific ...