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Citations for Thermo Fisher :
1551 - 1600 of 10000+ citations for Respiratory syncytial virus RSV fusion protein exodomain human heterodimeric IgG1 Fc tag recombinant antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 15% dialyzed FCS (Gibco), 1% penicillin-streptomycin ...
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bioRxiv - Cancer Biology 2020Quote: ... 15% fetal calf serum (FCS, Invitrogen), 450 μM monothioglycerol ...
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bioRxiv - Immunology 2020Quote: ... containing 10% Heat Inactivated FCS (Invitrogen) and 1% Penicillin-Streptomycin (Merck) ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% decomplemented FCS (Gibco). Cell lines were not authenticated after purchase other than confirming the absence of mycoplasma contamination ...
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bioRxiv - Immunology 2020Quote: ... containing 0.5% Heat Inactivated FCS (Invitrogen) and 1% Penicillin-Streptomycin (Merck) ...
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bioRxiv - Bioengineering 2021Quote: ... biotin anti-FC (Thermo Fisher, A18821), by mixing the nanovial concentrate and the antibody solution at a 1:10 ratio and incubating for 10 min ...
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bioRxiv - Immunology 2021Quote: ... with 5% FCS (Thermo Fisher Scientific). The dilutions were subsequently mixed with equal volumes of 4000 TCID50/ml SARS-CoV-2 (200 TCID50/ well ...
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bioRxiv - Immunology 2020Quote: ... containing 10% Heat Inactivated FCS (Invitrogen) and 1% Penicillin-Streptomycin (Merck) ...
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bioRxiv - Immunology 2020Quote: ... anti Fc primary antibody (ThermoFisher, USA), Alexa Fluor® 594 conjugate secondary antibody (ThermoFisher ...
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bioRxiv - Immunology 2022Quote: ... supplemented 10% FCS (Thermo Fisher Scientific) and 1 % ampicillin at a cell density of 3×106/mL per condition ...
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bioRxiv - Immunology 2022Quote: ... and 1:200 Fc block (Invitrogen) in 1×PBS (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... medium supplemented with 10% FCS (Invitrogen), 100 µg/ml streptomycin and 100 U/ml penicillin (Sigma) ...
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bioRxiv - Genomics 2024Quote: ... both supplemented with 10% FCS (Invitrogen), 2 mM glutamine (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... 10% Fetal Calf Serum (FCS, Gibco) and 0.05 mM β-mercapto-ethanol ...
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bioRxiv - Immunology 2022Quote: ... + 1% FCS + 5 mM EDTA (Gibco)) and incubated with 5μl TruStain FcX receptor blocking solution (Biolegend ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% FCS (Gibco, 10270). Stable cell lines were created either using Epac-SH250 biosensor or using Epac-SH201 biosensor18 ...
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bioRxiv - Immunology 2023Quote: ... A Fc-XP column (ThermoFisher, 494371201) containing anti-IgG-CH3 matrix which binds to the CH3 region (on Fc region ...
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bioRxiv - Neuroscience 2024Quote: ... 10% fetal calf serum (FCS) (Gibco), 1× penicillin/streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... This construct was expressed as a secreted protein by a stable Drosophila S2 cell line (30) and affinity purified using C-tag affinity resin (Thermo Fisher Scientific) followed by a size-exclusion chromatography polishing step in 20 mM Tris ...
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bioRxiv - Neuroscience 2020Quote: ... Protein mixtures (three replicates for each condition) were digested and labeled by tandem mass tags (TMT) 6plex reagent (Thermo Fisher Scientific, 90061), according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... Recombinant S and RBD trimeric proteins secreted in cell culture supernatants were purified using the CaptureSelect™ C-tag Affinity Matrix (ThermoFisher Scientific). Concentration of purified recombinant Abs were determined by PierceTM BCA Protein Assay Kit (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... transgenes were recombined with the Drosophila Gateway Vector Collection (DGVC) destination vectors expressing the N terminal tag fused target proteins under Actin5C promoter (Invitrogen, Carlsbad, CA). In addition ...
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bioRxiv - Neuroscience 2024Quote: Total HEK293 cell extracts were prepared according to the manufacture’s protocol (Pierce HA-Tag Magnetic IP/Co-IP kit and Pierce Protein A/G Magnetic Beads, Thermo Fisher Scientific, MA). Twenty- four hours after transfection ...
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bioRxiv - Cell Biology 2024Quote: ... The precipitated proteins were visualized by SDS-PAGE and Western blot analysis using an anti-His tag monoclonal rabbit antibody (Thermo Fisher Scientific) to determine the protein distribution in each fraction.
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bioRxiv - Biochemistry 2024Quote: ... HER2 with a C-terminal tail truncation (λ−1030–1255) followed by maltose binding protein (MBP) and twin-strep tags was cloned into pFastBac1 with a CMV promoter (Thermo Fisher Scientific). Point mutations were introduced in the HER2 kinase domain ...
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bioRxiv - Biochemistry 2023Quote: ... of randomized RNA library produced for IVM-seq experiment was incubated with equal molarity of 6x His-tag fused target protein complex immobilized on 7 μL Ni-NTA magnetic agarose beads (Thermo Scientific 78605) in the SELEX binding buffer (10 mM Tris pH 8.0 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... mammalian expression vector with an amino-terminal protein C tag followed with a 3C protease cleavage site and transfected into Expi293 cells (Thermo Fisher Scientific) using FectoPRO (Polyplus-transfection ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein bands were visualized using a LI-COR Odyssey FC Imaging system and Super Signal West Pico Chemiluminescent Substrate (Thermo Fisher). Western blots were quantified using Image Studio Software ...
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bioRxiv - Immunology 2020Quote: SARS-CoV-2 S and ACE2-Fc proteins were produced in Expi293F cells grown in suspension using Expi293F expression medium (Life Technologies) at 33°C ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 S and ACE2-Fc proteins were produced in Expi293F cells grown in suspension using Expi293F expression medium (Life Technologies) at 33°C ...
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Evolutionary stasis of an RNA virus indicates arbovirus re-emergence triggered by accidental releasebioRxiv - Evolutionary Biology 2019Quote: ... and virus isolates using Trizol LS® (Invitrogen, USA) and purified using Direct-zol RNA MiniPrep (Zymo Research ...
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bioRxiv - Cell Biology 2022Quote: ... or Sendai virus system Cytotune 2.0 (Thermo Scientific #A16517). Resulting iPSCs were clonally picked and expanded in mTESR (Stemcell Technologies ...
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bioRxiv - Microbiology 2019Quote: ... The virus inoculum was prepared in OPTI-MEM (Gibco), and cells were incubated in SMEM at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Pelleted virus was resuspended in PBS and TRIzol (Invitrogen) extracted ...
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bioRxiv - Microbiology 2022Quote: ... and from virus in supernatant using Trizol LS (ThermoFisher). A one-step qRT-PCR kit with SYBR green from Agilent was used ...
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bioRxiv - Microbiology 2021Quote: ... virus stocks were diluted in OptiPRO SFM (ThermoFisher Scientific) serum-free medium according to the desired MOI ...
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bioRxiv - Microbiology 2020Quote: ... The virus pellet was washed in HBSS (Thermo Fisher), and centrifuged at 100,000 × g (Beckman SW 32 Ti rotor ...
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bioRxiv - Immunology 2022Quote: ... and Moloney murine leukemia virus (MMLV) reverse transcriptase (Invitrogen), followed by RT-PCR with Fast SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... Immobilised virus was fixed with 4% formaldehyde (Thermo Scientific) in phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2020Quote: Virus stocks were prepared by transfecting 293FT cells (Invitrogen) seeded in 10 cm cell culture dishes with the viral constructs described above ...
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bioRxiv - Microbiology 2024Quote: ... and TaqMan Fast Virus 1-Step Master Mix (ThermoFisher). Reactions were set up according to the manufacturer’s protocols using a 10 µl reaction volume and 25 ng of total RNA template ...
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bioRxiv - Cancer Biology 2024Quote: ... virus was prepared in high-glucose DMEM (Gibco, #11965092) supplemented with 10% FBS and 1% penicillin–streptomycin ...
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bioRxiv - Microbiology 2023Quote: ... TaqMan Fast Virus 1-step Master Mix (Applied Biosystems) and primer/probes targeting NS3 primers/probe (IDT)11 ...
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bioRxiv - Genomics 2024Quote: The pseudotyped virus was generated from HEK293T cells (Invitrogen) by co-transfection with human immunodeficiency virus backbone plasmids expressing firefly luciferase as described previously ...
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bioRxiv - Microbiology 2021Quote: ... MHV-A59 was quantified by RT-qPCR by amplifying a fraction of its ORF5 gene for the structural protein M.15,16 The TaqMan™ Fast Virus 1-Step Master Mix Kit (Thermo Fisher Scientific Inc., 4444432) was used for RT-qPCR ...
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bioRxiv - Immunology 2021Quote: The RBDwt protein was conjugated to Cucumber mosaic virus - tetanus toxoid (CuMVTT) using the linker Succinimidyl 6-(beta-maleimidopropionamido) hexanoate (SMPH) (Thermo Fisher Scientific, Waltham, USA). CuMVTT contains a powerful T cell epitope of the Tetanus toxin (TT ...
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bioRxiv - Biochemistry 2020Quote: ... coupled on-line to an Orbitrap Fusion or Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific, San Jose, CA). Firstly ...
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bioRxiv - Immunology 2021Quote: P1 protein was analyzed at C-CAMP’s Mass spectrometry-based proteomics facility using Orbitrap Fusion Tribrid Mass Spectrometer (Thermo Fisher Scientific, USA) using optimized protocol for in-gel and in-solution trypsin digested product.
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bioRxiv - Neuroscience 2021Quote: Bacterial expression constructs coding for His6/SUMO-tagged fusion proteins of rat Shank3 (residues 1-348, SPN-Ank) were generated in pET-SUMO (Thermo Fisher Scientific) as described before (17) ...
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bioRxiv - Biophysics 2021Quote: 100 nM CoV firefly luciferase fusion RNA was added to 12 µL of HeLa lysate reaction mix from an in vitro protein expression kit (Thermo Scientific, 88882), along with 0.5 µL of rocaglamide/RocA solution (Med Chem Express ...