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Citations for Thermo Fisher :
1851 - 1900 of 10000+ citations for Respiratory syncytial virus RSV fusion protein exodomain human heterodimeric IgG1 Fc tag recombinant antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... and pol μ antibodies were affinity purified from rabbit serum by coupling each full-length recombinant protein to AminoLink Coupling Resin (Thermo Scientific) and following manufacturer’s instructions for IgG purification.
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bioRxiv - Microbiology 2020Quote: Recombinant biotinylated proteins (sE, sE-cvD and DIII) were expressed at 28°C using ExpiFectamine™ 293 Transfection Kit (Thermo Fisher Scientific). Cell supernatant was harvested and dialyzed ...
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bioRxiv - Microbiology 2019Quote: ... 125ng of total recombinant protein was separated on 4-20% or 10-20% Novex WedgeWell Tris-Glycine precast gels (Thermofisher, Waltham, MA). For silver stain ...
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bioRxiv - Cell Biology 2020Quote: ... specimens were incubated with 10 nM purified recombinant POLArISact protein or 2-5 nM Alexa Fluor 488 Phalloidin (AF488-phalloidin, A12379, Thermo Fisher Scientific) and 10 mg/ml bovine serum albumin (BSA ...
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bioRxiv - Immunology 2020Quote: His-tagged recombinant mouse CD47 (as well as proteins and antibodies noted later) was labeled using AlexaFluor488 NHS Ester (Succinimidyl Ester, Thermo Fisher Scientific) reconstituted in anhydrous DMSO (dimethylsulfoxide ...
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bioRxiv - Immunology 2020Quote: ... Plates were then incubated with 1μg/ml of biotinylated recombinant ACE2 protein for 1 hour at room temperature followed by incubation with HRP-conjugated streptavidin (Thermo Fisher Scientific) for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... S1-RBD and ACE2 recombinant proteins were expressed in Expi293 cells for 5 days following transient transfection using Expifectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell cultures were centrifuged at 4500g for 40 min ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.4 picomol of folded reporter mRNA was mixed with 20 picomol of recombinant protein and 200 picomol of UltraPure BSA (Thermo Fisher # AM2618) in binding buffer (10 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... 28 fmol of folded reporter mRNA was mixed with 0-10 picomol of recombinant protein and 100 picomol of UltraPure BSA (Thermo Fisher # AM2618) on ice for 1 hr ...
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bioRxiv - Cell Biology 2023Quote: ... and Alt-R® CRISPR-Cas9 tracrRNA Atto550 labeled, IDT) and recombinant Cas9 protein (Alt-R® S. p. HiFi Cas9 Nuclease V3, IDT) using Lipofectamine™ CRISPRMAX (Invitrogen) and cultured for 48 h in the presence of an HDR enhancer (final concentration 20 μM ...
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bioRxiv - Microbiology 2023Quote: Concentration of recombinant proteins were determined by measuring absorbance at 280 nm using a NanoDrop 1000 and/or NanoDrop One (Thermo Fisher Scientific) considering the extinction coefficient and the molecular weight calculated with the ProtParam tool (http://www.expasy.org/tools/protparam.html).
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bioRxiv - Cancer Biology 2023Quote: ... Single guide RNAs (sgRNAs) were incubated together with recombinant Cas9 (TrueCut Cas9 Protein v2, 5ug/ul, Thermo Fisher Scientific, cat. no. A36499) for 15 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... AcGFP-JEVcore recombinant protein and Alexa Fluor 594 or 555-conjugated antibody (2 mg/mL, Thermo Fisher Scientific, as an injection marker) were microinjected into the cytoplasm at a final concentration of 1 mg/mL ...
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bioRxiv - Immunology 2024Quote: ... the microtiter plates were washed 5 × with T-PBS and then incubated for 1 hour at room temperature with 2 μg/ml of biotinylated recombinant protein G (PierceTM, Thermo Fisher Scientific) for recognition of IgG antibodies from human ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were then incubated for 3 h at 4 °C with 50 mL of Pierce Protein-G beads bound with mouse anti-Ty antibody (Invitrogen Ty1 Tag Monoclonal BB2). Beads were washed 6 times with wash buffer (200 mM Tris pH 9 ...
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bioRxiv - Biochemistry 2023Quote: ... Pre-incubated mixture of scFv and respective whole venom was mixed with high-performance His-tag binding cobalt resin beads provided with Pierce™ His Protein Interaction Pull-Down Kit (Thermo Fisher Scientific, USA) and incubated at 20°C for 12 hours to allow attachment of the complex on resin via His-tag ...
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bioRxiv - Genomics 2022Quote: ... the HA-tagged protein of interest was detected with 1:500 diluted HA Tag Alexa Fluor 647 conjugated mouse antibody (ThermoFisher, Catalog no. 26183-A647). After antibody incubation ...
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bioRxiv - Immunology 2022Quote: ... after which cells were permeabilised (0.1% Triton X100) and stained with an antibody recognising dengue virus envelope protein (Invitrogen MA1-27093, for DENV serotypes 1-3), pan flavivirus envelope (Millipore MAB10216 ...
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bioRxiv - Cancer Biology 2019Quote: ... Positive phage clones were verified for preferential binding to plate-bound Fc-iNKG2D.YA or Fc-iNKG2D.AF versus Fc-wtNKG2D by spot ELISA and bound phage detected with biotinylated M13 phage coat protein monoclonal antibody E1 (Thermo Fisher # MA1-34468) followed by incubation with streptavidin-HRP.
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bioRxiv - Molecular Biology 2021Quote: ... we cloned an EcoCas3 cDNA with a octa-histidine tag and a six asparagine-histidine repeat tag into a pFastbac-1 plasmid (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... ACE2-S-tag expression was detected by using a mouse anti-S-tag monoclonal antibody 6.2 (Invitrogen, Cat. No. MA1-981), and an HRP-conjugated goat anti-mouse IgG Fc secondary antibody (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... cDNA encoding mouse TCR 2B4 α (with c-Myc-tag) and β (with V5-tag) sequences with 2A sequence linking each other were cloned into pCDNA3.1/Zeo(+) vector (Life Technologies) using Not1 and Xho1 restriction enzymes ...
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bioRxiv - Biochemistry 2021Quote: ... Coverslips were washed three time with PBS before the incubation with Alexa Fluor 555 (for Myc tag) and/or Alexa Fluor 647 (for HA tag) conjugated secondary antibodies (Invitrogen/Thermo Fisher) diluted in PBSAT for 30 min at RT ...
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bioRxiv - Microbiology 2022Quote: ... The resulting PCR product was digested with SmaI and ligated in frame with SmaI a N-terminal 3xFLAG-tag and a C-terminal 6xhistidine tag into a modified and SmaI-digested pET101/D-TOPO expression vector (Thermo Scientific), yielding pET101-NanH2.
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bioRxiv - Microbiology 2022Quote: ... The resulting PCR product was digested with BsiWI and ligated in frame with a N-terminal 3 x FLAG-tag and a C-terminal 6 × histidine tag into a modified and BsiWI-digested pET101/D-TOPO expression vector (Thermo Scientific), yielding pET101-NanH1.
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bioRxiv - Molecular Biology 2023Quote: ... cells were washed in 1 mL PBS and 100 µL of Phos-Tag lysis buffer was added dropwise to the well (Phos-Tag lysis buffer recipe: RIPA (ThermoFisher 89900), 3X Halt protease and phosphatase inhibitor cocktail (ThermoFisher 78444) ...
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bioRxiv - Immunology 2024Quote: ITS61.01 with a C-terminal rhodopsin tag (C9 tag) and untagged ITS103.01 were produced in transfected Expi293 cells (ThermoFisher, cat. A14527) and purified on rProtein A Gravitrap columns (Cytiva Life Sciences ...
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bioRxiv - Microbiology 2020Quote: ... interfaced with an Orbitrap Fusion Lumos (Thermo Scientific). Each peptide fraction was pre-concentrated on an Acclaim PepMap 100 trapping column before separation on a 50-cm ...
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bioRxiv - Genomics 2019Quote: ... HPLC connected to an OrbiTrap Fusion (Thermo Scientific) mass spectrometer ...
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bioRxiv - Immunology 2019Quote: ... coupled to an Orbitrap Fusion Lumos (Thermo Scientific) mass spectrometer was used for the LC-MS analysis ...
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bioRxiv - Plant Biology 2019Quote: ... an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific) was coupled with an UltiMate™ 3000 UHPLC (Thermo Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... and Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific). MASCOT software version 2.6.2 (Matrix Science ...
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bioRxiv - Neuroscience 2020Quote: ... and Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific). MASCOT software version 2.6.2 (Matrix Science ...
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bioRxiv - Neuroscience 2020Quote: ... coupled to an Orbitrap Fusion (Thermo/Fisher Scientific) instrument ...
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bioRxiv - Biochemistry 2021Quote: ... coupled to an Orbitrap Fusion Lumos (Thermo Scientific). Peptides were separated with a gradient consisting of 56 minutes at 5-28% B ...
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bioRxiv - Systems Biology 2020Quote: ... a Orbitrap LUMOS Fusion mass spectrometer (Thermo Scientific) was equipped with an EasySpray ion source and connected to Ultimate 3000 nano LC system (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: An Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific), equipped with a nano-ion spray source was coupled to an EASY-nLC 1200 system (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: An Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific), equipped with a nano-ion spray source was coupled to an EASY-nLC 1200 system (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... tribrid mass spectrometer (Orbitrap Fusion Tribrid, ThermoFisher Scientific) using conditions optimized at the PRF ...
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bioRxiv - Molecular Biology 2022Quote: ... connected to an Orbitrap Fusion LUMOS (Thermo Scientific) at 500 nl/min flow rate ...
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bioRxiv - Molecular Biology 2020Quote: The GFP-fusion library (Invitrogen, catalog number: 95702) was used as the basis for the mini-screening [69] ...
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bioRxiv - Neuroscience 2020Quote: ... and Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific). MASCOT software version 2.6.2 (Matrix Science ...
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bioRxiv - Microbiology 2021Quote: ... a plasmid-based promoter fusion from Thermo Scientific E ...
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bioRxiv - Systems Biology 2021Quote: An Orbitrap Fusion Lumos Tribrid MS (Thermo Scientific) operated in data-dependent acquisition mode was used for all analyses ...
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bioRxiv - Developmental Biology 2021Quote: ... and analyzed on an Orbitrap Fusion Lumos (ThermoFisher; at Cell Signaling Technology ...
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bioRxiv - Plant Biology 2021Quote: ... coupled to an Orbitrap Fusion Lumos (Thermo Scientific) mass spectrometer was used for the LC-MS analysis ...
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bioRxiv - Cell Biology 2021Quote: ... tribrid mass spectrometer (Orbitrap Fusion Tribrid, ThermoFisher Scientific) using conditions optimized in the PRF ...
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bioRxiv - Cancer Biology 2022Quote: ... connected to an Orbitrap Fusion Lumos Tribrid (ThermoFisher). Eluted peptides were analysed on an Orbitrap Fusion Lumos Tribrid platform (instrument control software v3.3) ...
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bioRxiv - Genomics 2022Quote: ... connected to an Orbitrap Fusion LUMOS (Thermo Scientific). The data were acquired using 120,000 resolution for the peptide measurements in the Orbitrap and a top T (3 s ...
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bioRxiv - Zoology 2022Quote: ... into Orbitrap Fusion MS system (Thermo Fisher Scientific), coupled with a Dionex Ultimate 3000 nano HPLC (Thermo Fisher Scientific) ...