Labshake search
Citations for Thermo Fisher :
1601 - 1650 of 7935 citations for IL 12RB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... and a Vanquish Horizon UHPLC system (Thermo Scientific, Rockford, IL), with source parameters as follows ...
-
bioRxiv - Neuroscience 2021Quote: ... fixed with 4% formaldehyde solution (Thermo Scientific, Rockford, IL, USA) for 10 min at room temperature and incubated either with anti-S100A (1:400 ...
-
bioRxiv - Physiology 2022Quote: ... Eight volumes of cold acetone (ThermoFisher Scientific, Rockford, IL, USA) were finally used for protein precipitation at −20°C overnight ...
-
bioRxiv - Molecular Biology 2022Quote: ... Fifty microliters of Ultralink Streptavidin (Pierce Thermo Scientific Rockford, IL), previously washed with 1 ml of 1X SIR Hyb buffer was added to each sample and samples were rotated at room temperature for 2 hours ...
-
bioRxiv - Immunology 2022Quote: ... and IL-13 (Alexa Fluor 488, or PeCyanine 7, Invitrogen; or eFluor 660 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Extracted RNA was treated with DNase I (Thermo Scientific, IL), and reverse transcribed using the Iscript cDNA kit (Bio-Rad Laboratories ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 2 μg/ml fibronectin (Fisher Scientific, Hanover Park, IL). Differentiated sensory neurons were maintained in N2 medium with neuronal growth factors (10 ng/ml human β-NGF ...
-
bioRxiv - Cancer Biology 2020Quote: ... permeabilized and incubated with primary IL-6 antibody (Invitrogen-AMC0862) and Alexa 488 conjugated anti-mouse goat antibody (Invitrogen-A11001 ...
-
bioRxiv - Immunology 2019Quote: ... IL-1 alpha Mouse Uncoated ELISA Kit (ThermoFisher #88-501988); eBioscience Mouse IL-6 ELISA Ready-SET-Go! Kit (Fisher Scientific #50-112-8863 ...
-
bioRxiv - Biochemistry 2019Quote: Fisher Optima™ grade (Fisher Scientific, Hanover Park, IL, USA) solvents were used for sample preparation ...
-
bioRxiv - Cancer Biology 2021Quote: ... TNF-α and IL-1β were purchased from Life Technologies Europe (Bleiswijk ...
-
bioRxiv - Plant Biology 2020Quote: ... Detection was performed with ECL (Thermo Scientific, Rockford, IL, USA) according to the manufacturer’s instructions.
-
bioRxiv - Microbiology 2021Quote: ... IL-1β and TNF-α ELISA Kits from Invitrogen (ThermoFisher) were used as per the manufacturer’s instructions.
-
bioRxiv - Immunology 2021Quote: ... cells were pretreated with recombinant IL-13 (PMC0134, Thermo Scientific) at 20ng/mL and IL-4 (PMC0045 ...
-
bioRxiv - Neuroscience 2020Quote: ... or nicotine tartrate (Acros Organics, Thermo Fisher Scientific, Chicago, IL) were mixed with tap water to the concentrations reported for each experiment ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: IL-8 secretion was quantified by ELISA (88-8088, Invitrogen) as a measure of TLR4 activation in HEK-null2 and HEK-hTLR4 cells as recommended by the provider (Invivogen ...
-
bioRxiv - Plant Biology 2022Quote: ... 1 mL of RIPA buffer (Thermo Scientific Pierce, Rockford, IL) containing 1× Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Scientific Pierce ...
-
bioRxiv - Immunology 2022Quote: ... or FUIGW-IL-10RB using Lipofectamine 2000 (Thermo Fisher Scientific) following the manufacturer’s protocols ...
-
bioRxiv - Cell Biology 2023Quote: ... SuperSignal West Femto chemiluminescent substrate (Thermo Scientific, Rockford, IL, USA) and ChemiDoc XRS+ (Bio-Rad Laboratories ...
-
bioRxiv - Cancer Biology 2023Quote: ... and 0.1% penicillin-streptomycin (Hyclone Labs, Thermo Scientific, Rockford, IL). MDA-PCa-2a cells were grown in plates/flasks coated with a fibronectin coating mix (AthenaES ...
-
bioRxiv - Molecular Biology 2023Quote: Cells were lysed with RIPA buffer (Thermo Scientific, Rockford, IL) containing a cocktail of protease and phosphatase inhibitors (Roche ...
-
bioRxiv - Immunology 2023Quote: ... IL-13 (eBio13A) conjugated to e660 or PE-Cy7 (ThermoFisher); Foxp3 (FJK-16s ...
-
bioRxiv - Immunology 2022Quote: ... biotin labeled anti-IL-4 (Thermo Fisher; 13-7042-85), anti-IL-17 (eBioscience ...
-
bioRxiv - Immunology 2023Quote: ... detection antibody (IFNγ, BD Biosciences #51-1818KA; IL-17A, Invitrogen #88-7371-88 ...
-
bioRxiv - Immunology 2023Quote: ... and 4 ng/ml IL-4 (Fisher Scientific, #404-ML) was added to each well ...
-
bioRxiv - Biochemistry 2023Quote: ... Pierce BCA protein assay kit (Thermo Scientific, Rockford, IL, USA) was used for total protein quantification of homogenates used for ELISAs ...
-
bioRxiv - Microbiology 2023Quote: ... was detected by chemiluminescence substrate (Thermo Scientific, Rockford, IL, USA) and visualized by ChemiDoc XRS+ (BioRad ...
-
bioRxiv - Immunology 2023Quote: ... Anti-mouse IL-13 antibody pair were purchased from Invitrogen. Standards for both IL-4 and IL-13 were purchased from BioLegend ...
-
bioRxiv - Genetics 2023Quote: ... the EASY-nLC chromatography system (Thermo Scientific, Rockford, IL, USA) was coupled on-line to an Orbitrap Elite instrument (Thermo Scientific ...
-
bioRxiv - Neuroscience 2024Quote: ... 20 ng/mL Interleukin-6 (IL-6) (Thermo Fisher Scientific), 20 ng/mL Interleukin-3 (IL-3 ...
-
bioRxiv - Bioengineering 2024Quote: ... and IL-1β (88-7013) were purchased from Invitrogen (USA). The Annexin-V/PI kit was purchased from BioLegend ...
-
bioRxiv - Cancer Biology 2024Quote: ... or hemocytometer and trypan blue (Fisher Scientific, Hanover Park, IL).
-
bioRxiv - Genetics 2024Quote: Mouse IL-6 Uncoated ELISA kit (cat# 88-7064; Invitrogen) was used to measure Interleukin-6 concentrations of mouse tissue lysates ...
-
bioRxiv - Immunology 2024Quote: ... followed by interleukin 2 (IL-2) (20 ng/mL, Gibco) stimulation for 5 days.
-
bioRxiv - Cell Biology 2020Quote: ... A Lsm12-KO cell line of HEK293 cells was generated with Synthego’s chemically modified sgRNA 5’-CCAGAAUGUCCCUCUUCCAG-3’ and GeneArt Platinium Cas9 nuclease (ThermoFisher Scientific) that were transfected together into cells using Lipofectamine CRISPRMAX Cas9 transfection reagent (ThermoFisher Scientific).
-
bioRxiv - Cancer Biology 2021Quote: ... After 24 hours the media was removed from the HEK293 CLK (NL-CLK1, CLK2-NL or CLK4-NL) transfected cells and replaced with 85 μL OPTI-MEM media (Gibco). NanoBRET Tracer 5 (Promega ...
-
bioRxiv - Molecular Biology 2020Quote: ... The wild-type complemented HEK293 RAD51 S14A mutant was generated by co-transfecting pcDNA5/FRT encoding wild-type RAD51 and pcDNA-DEST26 (Invitrogen), followed by single cell sorting (FACS ...
-
bioRxiv - Molecular Biology 2021Quote: HEK293 cells were purchased from ATCC and cultivated in DMEM medium supplemented with 10% fetal calf serum (Thermo Fisher Scientific). Cells were maintained at 37°C ...
-
bioRxiv - Neuroscience 2020Quote: The HEK293 cells (CRL-1573, ATCC, USA) were kept in the Dulbecco’s Modified Eagle Medium (High Glucose)-DMEM (Invitrogen, USA) supplemented with 10% fetal bovine serum-FBS (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2021Quote: ... HEK293 Phoenix cells (National Gene Vector Biorepository, Indianapolis, IN) were transfected with each construct using Lipofectamine 3000 (Thermo Fisher Scientific) per manufacturer’s recommendation ...
-
bioRxiv - Biochemistry 2019Quote: ... Transient transfection of 2N3T-Tip60 or the corresponding control empty vector was carried out for 48 hours into HEK293 cells using Lipofectamine 2000 transfection reagent (Invitrogen); UV irradiation was carried out at a dose of 15mJ/cm2 using Stratagene Stratalinker 1800 ...
-
bioRxiv - Neuroscience 2019Quote: HEK293 cells (ATCC Cat# CRL-1573) were maintained at 37° C and 5% CO2 in 1X DMEM medium (Thermo Scientific; Waltham ...
-
bioRxiv - Molecular Biology 2019Quote: Expression constructs for repeat RNAs or GFP-tagged codon-optimized DPRs were transfected in HEK293 cells or U-2 OS cells with Lipofectamine 2000 (Invitrogen) or FuGENE HD (Promega) ...
-
bioRxiv - Molecular Biology 2019Quote: ... pEGFP-C2-IQGAP1 and pcDNA3/RH-p122RhoGAP/DLC-1 plasmids were transfected into HEK293 cells using Lipofectamine 3000 (Invitrogen, MA).
-
bioRxiv - Microbiology 2021Quote: ... Plasmids encoding the SARS-COV-2 spike glycoprotein or its mutated variants were cloned into a G418 resistance gene containing backbone and transfected into HEK293 cells using Lipofectamine 2000 (Thermofisher) according to manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2021Quote: ... P2 or P3 baculovirus was produced in Sf9 cells and used to transfect HEK293S GnTI- suspension cells cultured in Freestyle 293 (Gibco) with 2% FBS at 37°C with 8% CO2 ...
-
bioRxiv - Neuroscience 2020Quote: ... The inter-molecular (oligomer) and intra-molecular (monomer-conformation) aSyn FRET biosensors were generated by transiently transfecting HEK293 cells using Lipofectamine 3000 (Invitrogen) with GFP-aSyn and aSyn-RFP (1:8 DNA plasmid concentration ratio ...
-
bioRxiv - Systems Biology 2021Quote: ... HEK293 and Ptk2 cell lines were cultured in DMEM (high glucose, pyruvate) supplemented with 10 % FBS and antibiotic-antimycotic (Invitrogen) and passaged routinely ...
-
bioRxiv - Systems Biology 2020Quote: HEK293 cell lines 293-F and Freestyle 293-F were cultivated in Freestyle 293 expression medium (Gibco, Thermo Fisher Scientific) at 37°C ...
-
bioRxiv - Systems Biology 2020Quote: HEK293 cell lines 293-F and Freestyle 293-F were cultivated in Freestyle 293 expression medium (Gibco, Thermo Fisher Scientific) at 37°C ...