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Citations for Thermo Fisher :
1551 - 1600 of 7935 citations for IL 12RB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... supplemented with 10% heat inactivated fetal bovine serum (HI-FBS, Life Technologies unless indicated), 100 U/mL penicillin-streptomycin ...
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bioRxiv - Microbiology 2020Quote: ... 3 μg of mammalian expression vector plasmids based on pEF1α V5 His C (Invitrogen) encoding codon-optimized gH ...
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bioRxiv - Microbiology 2019Quote: Qst was fused to a His-tag using the pEXP5-CT/TOPO vector (Invitrogen) following the TA-cloning protocol provided by the manufacturer ...
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bioRxiv - Immunology 2019Quote: ... 1xPen/Strep/Glu and 10% ultralow IgG HI-FBS (Thermo Fisher Scientific, Waltham, MA), and pelleted at 350xg for 5min ...
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bioRxiv - Biochemistry 2019Quote: ... A full length cDNA of Matriptase constructed in pcDNA3.1-V5-His (Thermo Fisher Scientific) was provided by Dr ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tagged proteins were purified using HisPur Ni-NTA resin (Thermo Fisher Scientific), at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... Then the blot was incubated with HRP conjugated anti-His antibody (Invitrogen, LOT 1902132) at 1:5000 dilutions for 16h at 40 C ...
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bioRxiv - Immunology 2021Quote: ... and the protein was purified on His-Pur Ni-NTA resin (Thermo Fisher Scientific) followed by size-exclusion chromatography ...
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bioRxiv - Cancer Biology 2020Quote: ... HA-tagged RON was cloned into the expression vector pcDNA3.1/V5-His-TOPO (Invitrogen) by fusion PCR ...
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bioRxiv - Microbiology 2020Quote: ... 1U Platinum High Fidelity (Hi-Fi) proof reading Taq polymerase (Invitrogen, Carlsbad, CA, USA). Two colonies of each G ...
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bioRxiv - Microbiology 2020Quote: ... using the primers shown in Table II and cloned into pcDNA-V5/His (Invitrogen). pCAGGS-EboGP-V5 (strain Zaire 1976 Mayinga ...
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bioRxiv - Immunology 2020Quote: ... The His-tagged proteins were purified with the HisPur Ni-NTA Resin (Thermo Fisher). To eliminate nonspecific binding proteins ...
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bioRxiv - Molecular Biology 2020Quote: ... His-tagged hAgo2 was purified from Sf9 cells using a baculovirus system (Thermo Fisher). Cells were lysed in Lysis Buffer (300mM NaCl ...
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bioRxiv - Microbiology 2022Quote: Pull-down assays were carried out using Dynabeads His-tag Isolation and Pulldown (Invitrogen). The 6His-tagged soluble pilin domains were used as bait ...
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bioRxiv - Biochemistry 2022Quote: ... and cultured in house) were grown to 70% confluency in DMEM-Hi glucose (Gibco) supplemented with 10% heat-inactivated foetal bovine serum (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR amplicons were digested and ligated into pcDNA3.1 myc-His B expression vectors (Invitrogen), with a 3xFlag epitope tag also fused to the N-terminus of Ptchd1 ...
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bioRxiv - Biochemistry 2022Quote: ... His-tagged protein was labeled with Alexa Fluor 488 NHS Ester (Thermo Fisher Scientific) per manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2022Quote: ... redundant anti-His antibody was washed away and 15 μL chemiluminescence substrate (Thermo Scientific) was added onto each well to generate chemiluminescence signal ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI FBS, Gibco™ Cat. #10438026) and 1% antibiotic/antimycotic (Gibco™) ...
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bioRxiv - Plant Biology 2022Quote: ... and purified by Dynabeads™ His-Tag Isolation and Pulldown (ThermoFisher, Catalog number 10103D). DA1 swapped protein ubiquitylation reactions used E1 ...
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bioRxiv - Molecular Biology 2023Quote: ... incubated for 30 minutes on ice with His-Pur Ni-NTA resin (Thermo Scientific), packed to a height of approximately 5 cm in polypropylene columns ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and probed with 6x-His Tag Monoclonal Antibody (HIS.H8) (Thermo Fisher Scientific #MA1-21315) diluted in 5% milk in PBS-T (1:500) ...
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bioRxiv - Molecular Biology 2022Quote: ... Junction PCR was performed with Extensor Hi-Fidelity PCR Master Mix (Thermo Fisher Scientific) in a 20 μL reaction using 500 nM of target-specific primers and the following cycling conditions ...
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bioRxiv - Immunology 2023Quote: ... M4 cells are maintained in DMEM/F12 media with 5% HI HS (Gibco #26050088).
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bioRxiv - Neuroscience 2023Quote: ... and subcloned into the pcDNA™3.1/myc-His(−)A vector (Thermo Fisher Scientific). We accidentally obtained the pcDNA™3.1/myc-His(−)A-(G4C2)9 vector at this step ...
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bioRxiv - Bioengineering 2023Quote: ... were first coated with 100 ng of mouse anti-His antibody (Invitrogen, MA1-21315) and blocked ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We then used the Dynabeads His-Tag Isolation and Pull- down kit (ThermoFisher 10103D) to isolate his-tagged Fcy1 following manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Supernatant was then processed through a His-tag cobalt resin (ThermoFisher, Waltham, MA, USA) following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... and His-p115 were affinity purified using Ni-NTA HisPur beads (Thermo Fisher Scientific) from crude E ...
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bioRxiv - Microbiology 2023Quote: ... A standard curve of 10-fold dilutions of plasmid pAc5.1-V5-His-A (Invitrogen) containing the DCV ORF-2 sequence was used to convert Ct values to genome copy numbers.
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bioRxiv - Cell Biology 2024Quote: ... The His-tagged dAsapPZA protein was expressed and purified following manufacturer guidelines (Invitrogen, USA).
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bioRxiv - Cell Biology 2020Quote: ... mitochondria isolation kit and IL-6 kit were from Invitrogen and were purchased from ALT Ukraine Ltd (representative of Thermo Fisher Scientific in Ukraine).
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bioRxiv - Immunology 2020Quote: Mouse IL-6 was quantified by uncoated ELISA Kit (ThermoFisher) according to manufacturer’s instruction.
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bioRxiv - Molecular Biology 2021Quote: ... SRT2104 (InvivoChem, Libertyville, IL) was (ACROS Organics, Fair Lawn, NJ); SRT2104 and vehicle (warm corn oil alone ...
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bioRxiv - Molecular Biology 2021Quote: ... medium supplemented with 25 ng/ml IL-3 (PHC0031, ThermoFisher), 2 mM Glutamax (35050061 ...
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bioRxiv - Physiology 2022Quote: ... SuperSignal West Femto chemiluminescent substrate (Thermo Scientific, Rockford, IL, USA) and ChemiDoc XRS+ (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... and visualized by ECL (Pierce, Thermo Scientific, Rockford, IL, USA). The ratios of F-actin or G-actin in hippocampal slices were calculated according to the density.
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bioRxiv - Neuroscience 2021Quote: ... and visualized by ECL (Pierce, Thermo Scientific, Rockford, IL, USA). Rac/Cdc42 or RhoA activation values were expressed as the ratio of Rac1-GTP ...
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bioRxiv - Molecular Biology 2021Quote: ... BCA protein assay kit (Thermo Fisher Scientific, Rockford, IL, USA). Sodium taurocholate hydrate ...
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bioRxiv - Neuroscience 2019Quote: ... rabbit anti-TGR5 (1:250, Thermo Fisher Scientific, Rockford, IL) and anti-GFAP (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... an interleukin-8 (IL-8) ELISA kit (Invitrogen, Catalog # CHC1303) to quantify secreted IL-8 levels in conditioned media ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Nicotine tartrate salt (Acros Organics, Thermo Fisher Scientific, Chicago, IL) was mixed with tap water to the concentrations reported for each experiment ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Nicotine tartrate salt (Acros Organics, Thermo Fisher Scientific, Chicago, IL) was mixed with tap water to the concentrations reported for each experiment ...
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bioRxiv - Cancer Biology 2019Quote: ... and developed using chemiluminescent substrate (Thermo Scientific, Rockford, IL, #34077).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Mouse ELISA Interleukin (IL)-6 kit was purchased from Invitrogen, Philippines ...
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bioRxiv - Neuroscience 2019Quote: ... and 1% penicillin-streptomycin-glutamine (Thermo Fisher Scientific, Rockford, IL). Approximately two-thirds of a single ganglia were placed for S (soma ...
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bioRxiv - Cell Biology 2021Quote: ... with western blotting filter paper (ThermoFisher Scientific, Rockford, IL, #84783). Blots were blocked using 5% nonfat dairy milk (Research Products International Corp ...
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bioRxiv - Microbiology 2022Quote: ... IL-4 cytokine was quantified using a kit (Thermo Fisher) following supplier’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... or nicotine tartrate (Acros Organics, Thermo Fisher Scientific, Chicago, IL) were mixed with tap water to the concentrations reported for each experiment ...
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bioRxiv - Microbiology 2021Quote: A BCA protein assay kit (Thermo Scientific, Rockford, IL, USA) was used to determine purified dentilisin sample concentrations as described above ...