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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for Human KIRREL shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Single colonies were picked and cultured overnight in LB medium + 50 µg/mL kanamycin and recombinant plasmids isolated using the GeneJET Plasmid Miniprep Kit (Thermo Fisher #K0503). Insert sequences in each plasmid were confirmed by Sanger sequencing ...
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bioRxiv - Cancer Biology 2023Quote: ... The cloned lentiviral CRISPR plasmids were verified by Sanger sequencing and co-transfected with LV-MAX packaging plasmids mix (Thermo Fisher, #A43237) into 293T cells using Lipofectamine 3000 transfection reagent (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: The NF-κB firefly luciferase reporter plasmid (Bayotime, Shanghai, China) and renilla luciferase reporter plasmid were co-transfected into 293T cells using Lipofectamine 3000 (Thermo Fisher Scientific). Firefly and Renilla luciferase activities were measured using the Dual-Glo Luciferase Assay System (Promega) ...
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bioRxiv - Cancer Biology 2023Quote: ... 6 million HEK 293FT cells were seeded and transfected after 24 hours with the LentiCRISPRV2-IL11g1 or LentiCRISPRV2-IL11g2 construct together with the pLp Δ8,9 packaging plasmid and VSVG envelope plasmid using lipofectamine™ 2000 transfection reagent (Life Technologies, 11668019) and Opti-MEM reduced serum medium (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... pLVX-DsRed-Spartin plasmid together with psPAX2 and VSV-G plasmid were transfected into HEK293 cells using Lipofectamine 3000 (L3000015; Thermo Fisher Scientific) for generating lentivirus ...
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bioRxiv - Bioengineering 2024Quote: ... For endogenous RNA trans-splicing experiments 400 ng of dCas13 expression plasmid was mixed with 400 ng of rcRNA expression plasmid 10ul R Buffer (Thermo Fisher Scientific). For experiments where only dCas13 expression plasmid or rcRNA plasmid was transfected ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting Gateway entry plasmids were further recombined with the pCDH-EF1a-Gateway-IRES-BLAST destination plasmid using the Gateway LR recombination kit (Invitrogen, cat. #11791020). All cloning vectors were kindly supplied by Dr ...
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bioRxiv - Microbiology 2024Quote: ... The pEcgRNA-guide plasmids from an overnight culture of Stellar cells expressing the plasmids grown in LB medium with spectinomycin (50 µg/ml) were extracted using GeneJET Plasmid Miniprep Kit (Thermo Scientific™). The pEcgRNA-guide plasmids were individually cloned into LT2c ΔStyLTI competent cells harboring the pEcCas plasmid and plated on both spectinomycin for selection of pEcgRNA (50 µg/ml ...
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bioRxiv - Biochemistry 2024Quote: ... 2 million cells were plated in 10-cm tissue culture plates and co-transfected the next day with 475 ng of plasmid encoding bxb1 recombinase and 7125 ng of the plasmid library using Lipofectamine 3000 (Invitrogen, Carlsbad, CA). Cells were grown at 33 °C for 4 days after transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... Microarray was performed by the UVA DNA Sciences core using the Human Affymetrix GeneChip Array ([HuGene-2_1-st] Affymetrix Human Gene 2.1 ST Array). Gene lists were generated from microarray data corresponding to genes >1.25 LogFC and <0.75 logFC were used in downstream analyses ...
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bioRxiv - Microbiology 2022Quote: Cytokines in the cell culture supernatants were measured using the human cytokine storm 21-plex procartaplex panel human magnetic bead Luminex assay (Thermo Fisher, EPX210-15850-901), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Concentrations of FGF19 in human serum samples were tested using a commercial Human Fibroblast Growth Factor 19 Assay Kit (Thermo scientific, Waltham, MA, USA). The level of serum C4 was quantified by a mass spectrometry-based method developed by our group.32
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bioRxiv - Molecular Biology 2019Quote: ... was performed using the TaqMan gene expression assays (EFHA1/Micu2 rat: s235193; EFHA1/Micu2 human: s47976; Hprt1 rat: Rn01527840; Hprt1 human: Hs99999909, Applied Biosystems, Life Technologies, Carlsbad, CA), using a 7900HT Fast Real-Time System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: ... Two control samples from the same pool of total human brain RNA (Ambion FirstChoice human brain reference RNA Cat#AM6050, Life Technologies Inc., Carlsbad, CA) were included with each of the five datasets ...
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bioRxiv - Cancer Biology 2021Quote: Transient plasmid transfection was performed using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were transfected using Lipofectamine 3000 (Life Technologies Invitrogen) following the instructions of the manufacturer.
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were transfected using Lipofectamine 3000 (Life Technologies Invitrogen) following the instructions of the manufacturer.
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bioRxiv - Cell Biology 2020Quote: ... Plasmid transfections were performed using Lipofectamine 2000 (ThermoFisher, 11668019) or Calcium Phosphate (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... the plasmid was added to 100µl of OptiMem (Invitrogen) along with 2.5µl of P3000 and combined with the tube containing LF3000 and incubated for a further 10 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were diluted in 1 ml of OptiMem (Gibco) and 20 μl polyethylenimine (PEI ...
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bioRxiv - Genetics 2021Quote: ... Plasmid construction was completed using GATEWAY cloning (Life Technologies) from PCR amplified products with the primer pair yoaApromoterGWF 5’-ggggacaagtttgt acaaaaaagc aggcttcCATTTTGTCCTCA TTATACTTCC AT-3’ and yoaApromoterGWR 5’-ggggaccact ttgtacaaga aagctgggtc ACTACCCCCT GTTGATTTGA ACAGG-3’ ...
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bioRxiv - Molecular Biology 2020Quote: ... and transfected with desired plasmids using Lipofectamine 2000 (Invitrogen). Cells were harvested 24 h later and assayed with the Dual-Glo Luciferase Assay System (Promega) ...
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bioRxiv - Genomics 2020Quote: ... and 7.5ug plasmid library using Lipofectamine 2000 (Life Technologies). Viral particles were collected 48 hours post-transfection and concentrated using Lenti-X Concentrator (Takara) ...
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bioRxiv - Molecular Biology 2022Quote: Template plasmids were digested using Not1 (Thermo Scientific, USA) to generate linearized DNAs ...
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bioRxiv - Neuroscience 2021Quote: ... Plasmids for injection were assembled using Gateway recombination (Invitrogen). Entry clones for Gateway reactions were generated by Gibson Assembly (Gibson et al. ...
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bioRxiv - Neuroscience 2021Quote: ... a 6XHis tag and SUMO protein expression plasmid (Invitrogen), with a TA cloning site ...
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bioRxiv - Cell Biology 2022Quote: ... plasmids were transfected with Lipofectamine 3000 (Invitrogen, Cat# L3000015) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... after which the GeneJET Plasmid Miniprep Kit (ThermoFisher Scientific) served for plasmid isolation from 2 ml of the O/N culture ...
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bioRxiv - Genetics 2019Quote: ... Plasmids were propagated in ccdb survival cells (Life Technologies) with chloramphenicol and ampicillin ...
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bioRxiv - Developmental Biology 2019Quote: ... Plasmid transfection was performed using Lipofectamine 2000 (Life Technologies). 24 hours after transfection ...
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bioRxiv - Cancer Biology 2019Quote: ... plasmids were transfected into HEK293T cells (Thermo Fisher Scientific) using FuGENE HD (Promega ...
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bioRxiv - Cancer Biology 2019Quote: ... Plasmids and miRNA were transfected with Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: Plasmid transfections were accomplished using Lipofectamine 3000 (Invitrogen, Inc) as recommended by the manufacturer ...
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bioRxiv - Neuroscience 2019Quote: ... sgRNAs were transcribed from linearized template plasmids (Ambion MEGAscritp), and Cas9 mRNA was transcribed in vitro from linearized template plasmid pMLM 3613 (Addgene Plasmid #42251) ...
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bioRxiv - Physiology 2019Quote: ... along with β-Gal plasmid (Ambion Cat. No# 5791). After 12 hours of transfection ...
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bioRxiv - Cell Biology 2019Quote: ... was inserted into pMT plasmid (Invitrogen, Thermo Fisher Scientific). pUbq-Spd2-GFP is kindly provided by Jordan Raff (University of Oxford ...
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bioRxiv - Cell Biology 2019Quote: ... was inserted into pMT plasmid (Invitrogen, Thermo Fisher Scientific). pUbq-Spd2-GFP is kindly provided by Jordan Raff (University of Oxford ...
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bioRxiv - Cell Biology 2020Quote: Plasmids were cloned into a pcDNA3.1 hygro(−) vector (ThermoFisher), digested by BamHI using a In-fusion HD cloning kit ...
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TrkB induced metastatic potential of cancer by suppression of BMP mediated tumor inhibitory activitybioRxiv - Cancer Biology 2020Quote: ... Plasmid transfections were carried out using Lipofectamine 2000 (Invitrogen) reagent according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2020Quote: Plasmid DNA pcDNA3.1+ vector was obtained from Invitrogen (#V79020), pSG5 vector was from Agilent (#216201) ...
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bioRxiv - Bioengineering 2021Quote: ... and target plasmids using Lipo 3000 (ThermoFisher, Waltham, USA). Fluid change was performed at 12 h and at 24 ...
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bioRxiv - Biochemistry 2020Quote: ... The expression plasmids were transfected into Expi293 cells (Invitrogen) for transient expression ...
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bioRxiv - Cancer Biology 2021Quote: ... packaging plasmids transfected with Lipofectamine 2,000 (Thermo Fisher Scientific). The Human GeCKOv2 A and B libraries in lentiGuide-Puro were amplified as described (Joung et al ...
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bioRxiv - Cancer Biology 2020Quote: ... or the GeneJet Plasmid Midiprep kit (ThermoFisher; Cat#: K0481). We validated that each LentiCRISPRv2 construct contained the correct guide via Sanger sequencing (Cornell Biotechnology Resource Center ...
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bioRxiv - Biochemistry 2020Quote: ... to plasmid DNA in Opti-MEM (Gibco, # 31985-062) at a ratio of 1:3 (μg vector DNA:μg PEI) ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmids were transformed into BL21 Star (DE3) cells (Invitrogen) for expression ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid transfections were performed using Lipofectamine 2000 (ThermoFisher, 11668019) or Calcium Phosphate (Promega ...
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bioRxiv - Systems Biology 2020Quote: ... Transfection grade plasmid was purified (PureLink HiPure Maxiprep, Invitrogen) and its guide arrays were sequenced to confirm complete and uniform library representation.
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bioRxiv - Microbiology 2021Quote: ... and EF1α-mCherry-N1 plasmid (Thermo Fisher Scientific, Singapore) were isolated using the Monarch® Plasmid Miniprep Kit (New England BioLabs Inc. ...
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bioRxiv - Microbiology 2021Quote: Plasmid transfections were performed using Lipofectamine 2000 (Life Technologies), at a ratio of 2 ug plasmid to 1 ul Lipofectamine in 100 ul opti-MEM ...