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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for Human KIRREL shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... target plasmid was transfected together with psPAX2 and pMD2.G packaging plasmids into 293T cells using lipofectamine 2000 transfection reagent (Invitrogen, 11668027). Media was changed 24h later and lentivirus supernatant was collected after an additional 24 h ...
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bioRxiv - Molecular Biology 2021Quote: ... BHK-21 cells were transfected 24 h prior to pseudotype inoculation with either empty plasmid or ACE2 expression plasmid (0.1 μg/well) using Lipofectamine LTX (Thermo Fisher Scientific). In order to study the antiviral activity of Camostat mesylate ...
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bioRxiv - Cell Biology 2019Quote: Stable cell lines were generated by co-transfecting the plasmid of interest together with a plasmid carrying the resistance gene for hygromycin with Cellfectin II (Life Technologies), and the selection process was started 2 d after transfection by supplementing the media with 250 μg/ml Hygromycin B.
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bioRxiv - Developmental Biology 2021Quote: ... a transposase expressing plasmid and a TOL2 plasmid expressing mCherry (as a transfection control) following the Lipofectamine 2000 protocol (Life Technologies). 48 hours post transfection ...
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bioRxiv - Cancer Biology 2019Quote: Lentiviral vector system includes the pLEX-MCS plasmid and the PMD2G and psPAX2 packaging plasmids (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Genetics 2020Quote: ... HEK293T cells were seeded on 48-well plates and transfected with SlugABEmax/SlugBE4max plasmid (300 ng) and gRNA plasmid (200 ng) using 1 ul of Lipofectamine 2000 (Life Technologies) in Opti-MEM(Gibco ...
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bioRxiv - Genetics 2020Quote: ... cells were seeded on 48-well plates and transfected with Cas9 plasmid (300 ng) and gRNA plasmid (200 ng) using 1 ul of Lipofectamine 2000 (Life Technologies) in Opti-MEM according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: The inducible XIST constructs within the HT1080 2-3-0.5a cells were modified by transiently transfecting two unique gRNA plasmids (pSPgRNA) and a Cas9 plasmid providing transient puromycin resistance (pSpCas9(BB)-2A-Puro) using Lipofectamine 3000 (ThermoFisher #L3000008). The day before the transfection the HT1080 cells were split into 24 well plates ...
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bioRxiv - Immunology 2020Quote: ... The 3T3 cells were transfected with Fsp I linearized CD40L plasmid (a kind gift from G. Freeman21,22) and Pvu I linearized pcDNA3-Neomycin plasmid) using Lipofectamine 2000 Reagent (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were co-transfected with sleeping beauty transposon plasmid pSBi-Hyg-BFP (hTMEM120A cDNA) and sleeping beauty transposase plasmid pCMV (CAT) T7-SB100 with Lipofectamine2000 (Life Technologies). Three days after transfection ...
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bioRxiv - Genetics 2022Quote: ... Plasmids and packaging plasmids psPAX2 and pMD.2G were co-transfected into cells using Lipofectamine™ 3000 Transfection Reagent (Invitrogen, L3000015). The medium was changed after 12 hour incubation ...
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bioRxiv - Neuroscience 2022Quote: ... and plasmids were purified 18 h after the inoculation with a 96-well plasmid purification kit (PureLink Pro, Thermo Fisher Scientific) following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: ... HEK293T and HCT116 cells were plated at a density of 20,000 cells per well in a 96-well plate and transfected with 200 ng of Cas13 expression plasmid and 200 ng of gRNA expression plasmid using Lipofectamine 2000 (Life Technologies) according to the manufacturer’s protocol DMEM (4.5 g/L glucose ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK293T cells were plated at a density of 200,000 cells per well in a 24-well plate and transfected with 800 ng of dCasRx expression plasmid and 800 ng of gRNA expression plasmid using Lipofectamine 2000 (Life Technologies) according to the manufacturer’s protocol in HEK media without penicillin/streptomycin ...
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bioRxiv - Cell Biology 2022Quote: ... Approximately two million colonies were scraped from plates and pooled and plasmid DNA was purified using PureLink® HiPure plasmid filter Midiprep kit (Invitrogen). The resulting plasmid library was transformed into TRy1392 pdr5∆ yeast strain ...
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bioRxiv - Molecular Biology 2023Quote: Extrachromosomal arrays were introduced by injection of 10 ng/μl plasmid DNA purified using the PureLink™ Quick Plasmid Miniprep Kit (Invitrogen) into the gonad of 1 DOA IR1284 (zcIs14[myo-3p::mtGFP] ...
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bioRxiv - Microbiology 2023Quote: ... The following day each well was co-transfected with 1 µg of replicon-encoding plasmid and 3 µg of C-prM-E-encoding plasmid using Lipofectamine 3000 (Cat# L3000-015; ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... cells at 80–90% confluency were co-transfected with 0.25 µg of single-sgRNA (or 4sgRNA) plasmids and 0.25 µg of dCas9-VPR plasmids using Lipofectamine 3000 (Thermo Fisher Scientific). Three days post-transfection ...
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bioRxiv - Microbiology 2023Quote: ... were co-transfected with plasmids encoding GFP-nsP3 fusions and the integrase-encoding plasmid using Lipofectamine™ 3000 according to the manufacturer’s recommendations (ThermoFisher Scientific). Clones of blasticidin-resistant ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 5 ml of the culture was pelleted by centrifugation and plasmid DNA was isolated using a GeneJET Plasmid Miniprep Kit (Thermo Scientific). The plasmid sequence was verified using BigDye 3.1 sequencing chemistry and an ABI3130 Genetic Analyzer ...
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bioRxiv - Molecular Biology 2023Quote: ... was used at a 3:1 ratio with 1 µg total plasmid (500 ng nLuc plasmid + 500 ng pGL4.13) in 100 µL in Opti-MEM (Thermo Fisher # 31985062). For 96-well plates ...
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bioRxiv - Cell Biology 2023Quote: ... co- transfected with lentiviral packaging plasmids (pCEP4-tat, pHEF-VSVG and pNHP) and lentiviral expression plasmids using Lipofectamine 2000 (Invitrogen, 11668019) as previously described50 with the following modifications ...
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bioRxiv - Developmental Biology 2023Quote: SOX2:GFP ESCs were plated into a gelatinised 6-well plates in SL and lipofected with the linearized Gata6:mCherry-Hygromycin plasmid and the CRISPR plasmid using Lipofectamine2000 (ThermoFisher 11668019) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... 18 μg of pMDLg/p.RRE Gag/Pol plasmid and 18 μg of pRSV.Rev plasmid alongside 15 μg of transfer vector plasmid encoding for CAR of interest using Lipofectamine 2000 (Thermo Fisher Scientific) and Opti-MEM (Gibco) ...
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bioRxiv - Neuroscience 2023Quote: ... HD patient iPSC-derived astrocytes were transiently transfected with 2 μg hFAN1 plasmid and control plasmid (pTT5 vector) by Lipofectamine 3000 (Invitrogen; L3000008) every week ...
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bioRxiv - Cancer Biology 2023Quote: Lentiviral packaging plasmids (pCMV-D8.9 and pCMV-VSVG) and reporter plasmids were transfected into HEK-293T cells using Lipofectamine 3000 (ThermoFisher cat#L3000001). Virus-containing supernatant was harvested at 48 and 72 hours after transfection and viral particles were concentrated with Lenti-X Concentrator according to the manufacturer’s instructions (Takara Bio cat#631232) ...
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bioRxiv - Molecular Biology 2023Quote: ... The mESCs were co-transfected with the 1.66 μg of the two sgRNA nickase-Cas9 plasmids and 0.83 μg of the HDR plasmid using the Lipofectamine LTX and Plus Reagent (Invitrogen, 15338-100) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The mESCs were co-transfected with the 1.66 μg of the two sgRNA nickase-Cas9 plasmids and 0.83 μg of the HDR plasmids using the Lipofectamine LTX and Plus Reagent (Invitrogen #15338-100). Transfected cells were first selected by 1 μg/ml puromycin for 2 days and subsequently selected by 250 μg/ml hygromycin for 6 days ...
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bioRxiv - Microbiology 2023Quote: Plasmid DNA from either wild type dengue plasmid or mutated dengue plasmid were quantified using a High Sensitivity Range Qubit (Thermo Fisher). Library preparation was carried out with the Nextera XT DNA Library Prep kit (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... transfections were performed by introducing 20 μg of targeting plasmid and 60 μg of linearised repair template (purified using Pure-Link™ HiPure Plasmid Midiprep Kit, Invitrogen) into ∽108 Percoll-enriched schizonts by electroporation using an Amaxa 4D Nucleofector X (Lonza) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1.5 μg dual sgRNA plasmids or 1 μg sgRNA plasmids and 1 μg homologous recombination donor using Neon™ NxT Electroporation System (Invitrogen). After 48 hr ...
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bioRxiv - Developmental Biology 2024Quote: ... 293 cells were transfected with plasmids encoding His-tagged recombinant proteins as described in the Plasmids section using lipofectamine 2000 (Invitrogen, #11668019) following the manufacturer’s instructions (four 100 mm plates each plasmid) ...
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bioRxiv - Cancer Biology 2024Quote: ... the SunTag plasmids and HMGB1-BFP (2 dishes) or Smad3-BFP plasmid (2 dishes) were simultaneously transfected with Lipo3000 transfection reagent (ThermoFisher Scientific). One dish of cells transfected with HMGB1-BFP ...
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bioRxiv - Immunology 2024Quote: ... pMDLg/p.RRE (18 μg) packaging plasmids and 15 μg of expression plasmids were mixed and transfected into 293T cells using Lipofectamine 3000 (Invitrogen #L3000150) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: The HEK293T packaging cells were transfected with OSA-expressing plasmids with lentiviral packaging plasmids using Lipofectamine Reagent 2000(Thermo Fisher Scientific). After 48 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 µg of each plasmid (gagpol, vsv-g, and transfer plasmid) were combined with 2 ml Opti-MEM I Reduced Serum Medium (Gibco 31985070) and 90 µl MirusBio TransIT-LT1 Transfection Reagent (Mirus MIR2306 ...
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bioRxiv - Biophysics 2024Quote: ... HEK293T cells were co-transfected with 200 ng pCAGEN plasmid for TDP-43 and 1000 ng pCAGEN plasmid for the other protein using Lipofectamine 3000 Transfection Reagent (Invitrogen, L3000001) in 12-well plates ...
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bioRxiv - Genomics 2021Quote: ... Lysates and positive technical controls (Human Universal Reference RNA - uhrRNA Agilent Cat# 740000, Santa Clara, California and Human Brain Total RNA brRNA - ThermoFisher AM7962, Waltham, Massachusetts), as well as no-cell negative controls (1X TempO-Seq lysis buffer alone ...
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bioRxiv - Immunology 2020Quote: The assay to measure total IgG was modified to measure IgG subclass responses at 1/100 including a two-step biotinylated anti-human IgG subclass (mouse anti-human IgG1: HP6069, IgG2: HP6002, IgG3: HP6050 IgG4: HP6023, Thermo fisher Scientific, UK) with a streptavidin-Phycoerythrin tertiary (Thermo fisher Scientific ...
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bioRxiv - Developmental Biology 2019Quote: ... Samples were run on the Clariom™ S Human Array to measure gene expression at >20,000 genes in the human genome (Affymetrix, Santa Clara, CA). Raw data generated from the arrays were read into R statistical software (version 3.5.1 ...
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bioRxiv - Cell Biology 2020Quote: ... Alexa Fluor™ 488-Human Transferrin conjugate (Cat# T13342) and DiI–human LDL conjugate (Cat# L3482) were obtained from Molecular Probes (Eugene, OR). Primer kits and Master Mix for RT-qPCR were purchased from Applied Biosystems (Foster City ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA levels were normalized to non-human primate β-actin (Table S1) or human β-actin (Hs99999903_m1, Applied Biosystems; Thermo Fisher Scientific) and calculated by the ΔΔCt method [46].
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bioRxiv - Microbiology 2021Quote: ... Viral RNA levels were normalized to non-human primate β-actin (Table S1) or human β-actin (Hs99999903_m1, Applied Biosystems; Thermo Fisher Scientific) and calculated by the ΔΔCt method [46].
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bioRxiv - Immunology 2021Quote: PBMC derived CD4+ T cells were isolated from HIV-infected MACS participants using EasySep™Human CD4+ T Cell Isolation Kit and activated overnight with Human T-Activator CD3/CD28 Dynabeads® (Life Technologies). The next day Dynabeads were separated from the CD4+ T cells by manual dissociation followed by magnet isolation ...
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bioRxiv - Immunology 2021Quote: ... CD4+ and CD8+ T cells were isolated by negative selection with the Dynabeads Untouched Human CD4 T Cells Kit and the Dynabeads Untouched Human CD8 T Cells Kit (Invitrogen, Thermo Fisher Scientific) respectively ...
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bioRxiv - Cell Biology 2022Quote: Human cerebral organoids are generated from human induced pluripotent stem cells (iPSCs) which were purchased from GibcoTM (Gibco by Thermofisher, Waltham, Massachusetts, USA). iPSCs were plated in a dish pre-coated with Matrigel® hESC-Qualified Matrix (Corning ...
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bioRxiv - Cell Biology 2023Quote: We profiled the expression of transcripts in human aortic vascular smooth muscle cells using the Clariom D human DNA microarray (Thermo Fisher Scientific Inc.). This platform includes information from probe sets representing known and predicted exons and introns on both strands of the genome that have been mapped to more than genes ...
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bioRxiv - Immunology 2023Quote: Customized cytokine 25-Plex Human ProcartaPlex Assay (customized from Cytokine 25-Plex Human ProcartaPlex Panel 1B with the catalog # EPX250-12166-901, Thermo Fisher Scientific, USA) was used to measure multiple cytokines in the supernatants of stimulated human PBMCs.
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bioRxiv - Evolutionary Biology 2020Quote: ... Three to six transformed colonies were cultured in 5 ml of LB medium and plasmids were subsequently purified with a GeneJET Plasmid Miniprep kit (Thermo Fisher Scientific). Purified plasmids were sequenced by Eurofins Genomics (Fig ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Large quantities of purified plasmids containing AsegIRs and AsegIR8a with verified sequences were obtained using the PureLinkTM HiPure Plasmid Filter Midiprep Kit (Thermo Fisher Scientific). cRNAs of AsegIRs and AsegIR8a were synthesized from NotI (Promega ...