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Citations for Thermo Fisher :
1451 - 1500 of 10000+ citations for Human KIRREL shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Metabolic specialization drives reduced pathogenicity in Pseudomonas aeruginosa CF clinical isolatesbioRxiv - Microbiology 2023Quote: ... previously coated with human type I collagen (Gibco, A1048301). ALI was established once cells reached full confluency by removing media from the apical chamber and replacing media in the basolateral chamber with Pneumacult-ALI maintenance medium (STEMCELL Technologies ...
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Tight junction membrane proteins regulate the mechanical resistance of the apical junctional complexbioRxiv - Cell Biology 2023Quote: ... rabbit polyclonal anti-human JAM-A (Invitrogen; #PA5-120157); rabbit polyclonal anti-CAR (kindly provided by Jeffrey M ...
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bioRxiv - Molecular Biology 2023Quote: Human HEK293T cells (ATCC) were maintained in DMEM (Gibco) supplemented with 10% heat inactivated FBS (Gibco ...
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bioRxiv - Immunology 2023Quote: ... 50 ng/mL human IL-21 (Thermo Fisher Scientific), 50 ng/mL human IL-4 (Miltenyi) ...
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bioRxiv - Immunology 2024Quote: ... with anti-human CD107a-eFluor660 (clone H4A3, Invitrogen, UK) at an E:T ratio of 1:1 for a total of 4 hours at 37°C ...
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bioRxiv - Genomics 2024Quote: ... 20 ng/mL human recombinant EGF (Thermo Fisher PHG0311), and 10% fetal bovine serum ...
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bioRxiv - Genetics 2024Quote: ... whereas a human CSNK2A2 pre-designed assay (Hs00176505_m1, ThermoFisher) was used as the housekeeping control.
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bioRxiv - Immunology 2024Quote: ... and CD3/CD28 Human T-activator Dynabeads (Thermo Fisher) at a 1:1 bead to cell ratio ...
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bioRxiv - Bioengineering 2023Quote: Human iPSC lines were cultured in StemflexTM medium (ThermoFisher) and passaged approximately every 3 days (1:8–1:12 split ratio) ...
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bioRxiv - Neuroscience 2023Quote: ... human Bmp7 (100 ng/ml, ThermoFisher Scientific, 120-03P) or human Semaphorin3d (400 ng/ml ...
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bioRxiv - Neuroscience 2023Quote: ... 2 ng/ml human Tgfb2 (ThermoFisher Scientific, 100-35B), 10 ng/ml murine Csf1 (Biolegend ...
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bioRxiv - Neuroscience 2023Quote: ... human Pleiotrophin (100 ng/ml, ThermoFisher Scientific, 450-15), human Midkine (100 ng/ml ...
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bioRxiv - Neuroscience 2023Quote: ... human Midkine (100 ng/ml, ThermoFisher Scientific, 450-16), human Bmp7 (100 ng/ml ...
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bioRxiv - Neuroscience 2024Quote: ... Cre-expressing Human embryonic kidney (HEK, Ambion #SC004-BSD) cells were transfected with plasmids expressing cDNA and either shLacZ or the shRNA against the target gene ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and goat anti-human AlexaFluor488 (1:500, Invitrogen A11013) secondary antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... anti-human PHF-tau (1:1000; Thermo Scientific, #MN1020), mouse anti-VGAT (1:500 ...
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bioRxiv - Genomics 2024Quote: Human iPSCs were cultured in Essential 8 Medium (Gibco/Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... and 10% heat-inactivated human AB-positive serum (Invitrogen) at 37°C in 5% CO2 ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 μg/ml of human ⍺-CD28 (Thermo Fisher Scientific) and 200 ng of IL-2 (Immunotools ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 μg/ml of human ⍺-CD3 (Thermo Fisher Scientific), 5 μg/ml of human ⍺-CD28 (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... 10 ng/mL recombinant human VEGF-165 (Gibco; PHC9391), 20 ng/mL recombinant human EGF (R&D system ...
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bioRxiv - Bioengineering 2024Quote: ... 10 ng/mL recombinant human VEGF-165 (Gibco; PHC9391), 20 ng/mL recombinant human EGF (R&D system ...
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bioRxiv - Cell Biology 2024Quote: ... human IL-6 Uncoated ELISA (88-7066-88, Invitrogen) and human GRO alpha (CXCL1 ...
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bioRxiv - Cancer Biology 2024Quote: ... growth factor 100ug/ml FGF10 (recombinant human) ( Thermo Fisher) and 500nM A-83-01 (Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: ... Human neonatal epidermal keratinocytes (HEKn, Thermo Fisher Scientific, C0015C) were cultured in EpiLife Medium (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Human neonatal dermal fibroblasts (HDFn, Thermo Fisher Scientific, C0045C) were cultured in Medium 106 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... which were then transfected with a combination of PG13-luc assay plasmid and a control plasmid pRL-SV40P (50:1) using Lipofectamine 2000 (Invitrogen, USA) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... guide RNA plasmid and a plasmid expressing SpCas9 at a ratio of 2:1:1 (total 12ug) using Neon electroporator (Life Technologies) and a 30ms ...
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bioRxiv - Cell Biology 2021Quote: ... hTERT-RPE1 cells were transfected with donor plasmid and AIO plasmid with the appropriate gRNAs using a Neon transfection system (Thermofisher Scientific). For 5 × 106 cells ...
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Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying essential circRNAsbioRxiv - Molecular Biology 2020Quote: ... Clones were scraped off the LB plates and plasmid DNA was extracted using PureLink™ HiPure Plasmid Maxiprep Kit (ThermoFisher, K210007). The libraries were submitted for next generation sequencing to confirm the coverage and diversity of gRNA and shRNA libraries ...
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bioRxiv - Developmental Biology 2020Quote: ... The next day PcDNA 3.1/ Twist1-BirA*-HA plasmid or PcDNA 3.1/ GFP-BirA*-HA plasmid was transfected into cells using Lipofectamine® 3000 (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... RPE-1 wildtype cells were transfected with the CRISPR/Cas9 plasmid containing the PCNA gRNA and the donor plasmid containing the K164R mutation using the Neon Transfection System (Invitrogen MPK5000) following standard protocols ...
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bioRxiv - Immunology 2021Quote: ... were transformed with the modified transfer vectors and before plasmid DNA extraction using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 1.5 μg of reporter plasmid and 15 ng of Cre-plasmid was mixed with 3 μL Lipofectamine LTX reagent (Invitrogen, #15338100), 1.5 μL PLUS reagent and 100 μL Opti-MEM following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2019Quote: ... This plasmid consists of six inserts and the plasmid cloning vector CloneJet pJET1.2/blunt (2974 bp; Thermo Fisher Scientific, Massachusetts, USA). For the inserts ...
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bioRxiv - Microbiology 2019Quote: Genomic DNA was extracted using the GeneJET Genomic DNA Purification Kit while plasmid DNA was extracted using the GeneJET Plasmid Miniprep Kit (Thermo Scientific). PCR assays were performed using Phusion High-Fidelity PCR Master Mix (Thermo Scientific) ...
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bioRxiv - Genomics 2019Quote: ... were used for sequencing the full length of cloned PCR product after plasmid purification using the GeneJET Plasmid Miniprep Kit (Thermo Scientific).
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bioRxiv - Bioengineering 2021Quote: ... nanobody sequences were PCR amplified with primers containing terminal regions of homology for cloning into a pRset plasmid (Addgene plasmid 3991)(Invitrogen, V35120) in frame and linked to mCherry ...
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bioRxiv - Neuroscience 2021Quote: ... Transient overexpression of VDR in SH-SY5Y cells was performed by transfecting a VDR expression plasmid (pcDNA3-VDR) or a mock control plasmid (pcDNA3) with Lipofectamine 2000 (Invitrogen, USA). For mammalian two-hybrid assays to assess the interaction between VDR and RXR ...
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bioRxiv - Systems Biology 2020Quote: ... We added 800–1000 ng of plasmid (SF2(gDNA) or SF2(cDNA) or SynT) using the Lipofectamine LTX plasmid transfection reagent (ThermoFisher Scientific) and changed the culture media to Opti-MEM™ Reduced Serum Medium (ThermoFisher Scientific) ...
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bioRxiv - Systems Biology 2020Quote: ... We added 800–1000 ng of plasmid (SF2(gDNA) or SF2(cDNA) or SynT) using the Lipofectamine LTX plasmid transfection reagent (ThermoFisher Scientific) and changed the culture media to Opti-MEM™ Reduced Serum Medium (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2021Quote: ... 1 million cells in 6-well plates were transfected with 2 μg of plasmid pT2_FF together with 2 μg of plasmid pCMV(CAT)T7-SB100x and 2 μg of plasmid pcDNA3.1-mCherry using Lipofectamine 2000 (Thermo Fisher, #11668027). After 24 h ...
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bioRxiv - Molecular Biology 2020Quote: ... K562#17 cells were transfected with 32 μg of barcoded IPR-PB-BC plasmid library and 6 μg of PB transposase plasmid using Lipofectamine 2000 (ThermoFisher #11668019). Mock-transfected (without PB transposase ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were extracted with NucleoSpin Plasmid Transfection-grade (Machery-Nagel) and concentration were measured on Nanodrop ND-1000 (Thermo Fisher Scientific). All plasmids were sequenced by Sanger method (Giga-Genomics).
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected with 1,250 ng of dCas13a m6A editor plasmid and 1,250 ng of crRNA plasmid using 5 μl of Lipofectamine 3000 (Thermo Fisher Scientific) in Opti-MEM I Reduced Serum Media (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected with 1,250 ng of dCas13a m6A editor plasmid and 1,250 ng of crRNA plasmid using 5 μl of Lipofectamine 3000 (Thermo Fisher Scientific) in Opti-MEM I Reduced Serum Media (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... The cDNAs were cloned into pDONR221 plasmid and introduced into the PB-Hyg-Dest plasmid using Gateway technology (Thermo Fisher Scientific). The PB-Hyg-Dest-mCherry-PAR-6B or the PB-Hyg-Dest-LifeAct-Ruby plasmid was co-transfected with the piggyBac plasmid using Lipofectamine 3000 to generate Hygromycin B resistant stable cell lines ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmid DNA from the overnight cultures was harvested via extraction with the PureLinkTM HiPure Plasmid Filter Midiprep kit (Thermo Fisher Scientific), and insert sequence was confirmed by Sanger sequencing at the on-site DNA Sequencing Facility (Dept ...
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bioRxiv - Biochemistry 2022Quote: ... The constructs were transformed into competent DH5α (E. coli) cells and plasmids were extracted using GeneJET plasmid miniprep kit (Thermo Scientific) to verify sequence integrity by sequencing (Eurofins Genomics) ...
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bioRxiv - Neuroscience 2022Quote: ... we cloned sequences encoding each BoNT light chain (from plasmids shared by Matt Kennedy, University of Colorado, USA) into the gateway vector donor plasmid (ThermoFisher, # K240020). We then transferred these sequences into the final pUASt destination vector from Carnegie Drosophila Gateway Vector Collection using the Gateway reaction kit (Thermofisher ...