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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... The 24-well plates with infected BHK-21 cells were overlaid with 1 ml of 0.8% methylcellulose in complete DMEM medium with 2% FBS and incubated for 5 days in a cell culture incubator (Thermo Fisher Scientific, 37°C and 5% CO2). Plaques were fixed and developed with staining reagent (1% crystal violet in 1:1 methanol/acetone solution ...
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bioRxiv - Molecular Biology 2021Quote: ... the input aliquot (100 µl) was mixed with equal volume of 2× protein sample buffer (2× NuPAGE LDS buffer [Life Technologies], 100 mM DTT, 4% [w/v] SDS) and the IP with 100μl 1 x protein sample buffer followed by incubation for 10 min at 75 °C ...
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bioRxiv - Cell Biology 2020Quote: Whole protein extracts were prepared from JJN3 pellets (4°C, 150 x g, 5 min) according to the cell extraction protocol for ELISA sample preparation by ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: Thymi from 4-week-old wild type mice were harvested and 5 million thymocytes per condition were cultured in IMDM medium (Gibco) with 2% FBS (HyClone Thermo Fischer Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... produced from DKO piglet were prepared and stained at 4°C for 45 minutes with Alexa 488-conjugated Griffonia simplicfolia isolectin IB4 (GS-IB4) (5 μg/mL, Invitrogen). The stained cells were washed twice and then analyzed by flow cytometer (BD Accuri™ C6 Plus) ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were boiled at 95°C for 5 min before running on a NuPAGE 4-12% Bis-Tris protein gradient mini gel (Invitrogen) at 200 V for 5 min in MOPS running buffer (Invitrogen ...
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bioRxiv - Genomics 2020Quote: Neuronal suspensions were labelled at 37°C with a combination of the Fluo-4 calcium indicator (5 μg/ml; Invitrogen) and either Alexa-Fluor 594-coupled Wheat Germ Agglutinin (WGA ...
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bioRxiv - Genetics 2020Quote: ... For morphological analyses 105 cells were resuspended in 200 ml PBS and spun (5 min, 400 rpm) in a Cytospin 4 (ThermoFisher), before staining with modified Wright’s Stain on a Hematek (Bayer Health Care) ...
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bioRxiv - Genetics 2021Quote: ... 100 µL of the cell suspension were then placed in a cytofunnel and spun at 1200 rpm for 5 min with high acceleration using a cytocentrifuge (Shandon Cytospin 4; ThermoFisher). For FISH ...
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bioRxiv - Microbiology 2021Quote: ... Samples were heated to 50°C for 5 minutes before being loaded on 4-12% Novex WedgeWell Tris-Glycine gels (ThermoFisher).
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bioRxiv - Bioengineering 2021Quote: ... 10 µg of each sample was boiled at 95°C for 5 min and loaded onto the 4-12% Bis-Tris Gel (NP0336, ThermoFisher). Gels were run for 1 hour and 15 min at 120 V in with NuPage MES Buffer (NP0002 ...
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bioRxiv - Biophysics 2021Quote: Cells were magnetically labelled thanks to an incubation of 2h with a solution of iron oxide nanoparticles at [Fe] = 4 mM and supplemented with 5 mM citrate in RPMI medium (Gibco). The viability and the proliferation of cells after magnetic labelling were assessed by Alamar Blue showing no impact of the magnetic labelling right after the labelling or after one day (Supplementary Figure 1).
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bioRxiv - Biochemistry 2021Quote: ... and samples were incubated at 70°C for 5 minutes and loaded onto Bis-Tris 4-12 % gradient gels (ThermoFisher). Bands were stained by CBB and relative band intensity was quantified by scanning and semi-automated analysis in ImageQuant (GE Healthcare).
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bioRxiv - Neuroscience 2021Quote: ... Sections were blocked in PBS-T containing 5% NDS and then incubated overnight at 4°C with rabbit anti-PSD95 (1:1000, Invitrogen/ThermoFisher) and guinea pig anti-vGlut2 (1:10,000 ...
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bioRxiv - Immunology 2020Quote: ... and anti-CD127-PE-Cy5 (clone eBioRDR5; 5 μL; cat. # 15-1278-42) all from Thermo Fisher (Supplementary Fig. 4). mAbs for chemokine receptors (i.e ...
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bioRxiv - Cell Biology 2021Quote: ... 5–12 µl of protein lysate was loaded into pre-cast protein gels (4–12 % Bolt Bis Tris Plus, ThermoFisher) together with 10 µl of the molecular marker (PageRuler prestained ...
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bioRxiv - Cell Biology 2021Quote: ... Human islets were loaded with 5 µg/µL of the Ca2+-sensitive dye Fluo-4 (1923626, Invitrogen, Thermo Fisher Scientific) for 60 min before being transferred to a recording chamber ...
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bioRxiv - Cell Biology 2021Quote: ... Human islets were loaded with 5 µg/µL of the Ca2+-sensitive dye Fluo-4 (1923626, Invitrogen, Thermo Fisher Scientific) for 60 min before being transferred to a recording chamber ...
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bioRxiv - Systems Biology 2021Quote: ... and samples were centrifuged at 16,000g for 5 minutes at −4°C in a Sorvall Legend Micro 17R microcentrifuge (Thermo Scientific). The supernatant (extract ...
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bioRxiv - Neuroscience 2020Quote: Culture medium was replaced with Fluo-4 AM (5 μM) or X-Rhod-1 AM (10 μM) (both from Invitrogen) working solution in Ca2+ solution (pH 7.4) ...
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bioRxiv - Cell Biology 2021Quote: ... samples were boiled for 5 minutes in NuPage LDS sample buffer and run on NuPage Bis-Tris 4-12% protein gels (Invitrogen), and proteins were transferred to PVDF membranes (Immobilon-FL ...
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bioRxiv - Pathology 2022Quote: ... for 5 min at 95 °C before they were loaded in Bolt™ 4–12 % Bis-Tris gels (Thermo Fisher). After separation ...
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bioRxiv - Biochemistry 2022Quote: ... A total of 405 WT peptides were identified (Table 4) on the Fusion Lumos OrbiTrap mass spectrometer (mass tolerance <5 ppm) using Proteome Discoverer 2.2.0 software (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2022Quote: ... to final concentration 0.5 mg/ml protein and 6x dye and heated from 10 to 70 °C with fluorescence reading every 0.1 °C (4 to 5 sec) in a QuantStudioTM 7 Flex Real-Time PCR System (Life Technologies). Protein thermal melting curve were generated using the Protein Thermal Shift TM software ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were boiled at 95°C for 5 min and loaded on SDS-PAGE (NuPAGE 4-12% Bis-Tris, Invitrogen). Efficiency of ligand coupling to the Ad capsid (% total hexon protein coupled ...
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bioRxiv - Cell Biology 2022Quote: ... and cells were passaged every 4-5 days using 0.5 mM EDTA (pH 8.0) (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Bioengineering 2022Quote: ... The dissociated cells and tissue fragments were centrifuged at 1,000 rpm for 5 mins at 4 ℃ and cultured in a T75 tissue-culture flask in a growth medium (advanced DMEM/F12 (Gibco) containing 5% bovine serum (Gibco)) ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were transferred onto a nitrocellulose membrane and then blocked overnight while shaken at 4 °C in TBST-5% BSA—a TBS Tween-20 (TBST; ThermoFisher) solution containing 5% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2020Quote: Total RNA was isolated from groups of 5 HIOs per replicate with a total of 4 replicates per infection condition using the mirVana miRNA Isolation Kit (ThermoFisher). Cytosolic and mitochondrial ribosomal RNAs were removed from samples using the Ribo-Zero Gold Kit according to manufacturer’s protocol (Illumina) ...
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bioRxiv - Microbiology 2022Quote: Hemolymph sporozoites collected on days 19 to 24 post-infection were centrifuged for 5 min at 450 x g and 4 °C in an 8-well Lab-Tek chamber slide (Nunc) or a 384-well plate (Greiner) ...
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bioRxiv - Physiology 2022Quote: ... then perfused the lungs with 3 mL of ice-cold DPBS containing Ca2+ and Mg2+ followed by 5 mL of 4% methanol-free formaldehyde (ThermoFisher) in DPBS containing Ca2+ and Mg2+ ...
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bioRxiv - Synthetic Biology 2020Quote: Samples were run on 4% agarose (Bio-Lab, Israel) in 1× TBE buffer (Biological industries, Israel) supplemented with 5 mM MgCl2 (Ambion), and stained with 0.3 µg/ml ethidium bromide (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... The loading solution contained a 1:10 mixture of 5 mM Fluo-4 AM in dry DMSO and PowerloadTM concentrate (Invitrogen), which was diluted 100-fold into extracellular Tyrode’s solution (NaCl 140mM ...
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bioRxiv - Physiology 2021Quote: ASM cells and arteries were respectively loaded with 5 μM and 10 μM Fluo-4-AM (Invitrogen, Thermo Fisher Scientific) in a buffer solution containing 140 mM NaCl ...
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bioRxiv - Physiology 2021Quote: ASM cells and arteries were respectively loaded with 5 μM and 10 μM Fluo-4-AM (Invitrogen, Thermo Fisher Scientific) in a buffer solution containing 140 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... boiled at 100°C for 5 min and then separated on NuPAGE Novex 4-12% Bis-Tris midi gels (Invitrogen) using NuPAGE MES SDS Running buffer (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: ... 5% DTT) for 10 min at 70 °C before being loaded on a 4-12% Bis-tris gel (ThermoFisher NP0322BOX) at 10 µg/lane ...
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bioRxiv - Biochemistry 2021Quote: The input (total Nav1.5 expression) and biotinylated (cell surface-expressed Nav1.5) samples were separated on NuPAGE 4–12% Bis-Tris gels (Invitrogen, ThermoFisher) and transferred to nitrocellulose membrane (iBlot transfer system ...
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bioRxiv - Biochemistry 2021Quote: ... and human phosphatidylinositol 4-phosphate 5-kinase type-1 beta (hPIP5KB; Uniprot #O14986) were derived from codon optimized genes synthesized by GeneArt (Invitrogen). The gene encoding yeast Mss4 (Uniprot #P38994 ...
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bioRxiv - Neuroscience 2021Quote: ... pH 6.8) for 5 min at 95° C and loaded to NuPAGE 4-12% Bis-Tris Protein polyacrylamide gels (Invitrogen, #NP0323BOX). After electrophoresis ...
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bioRxiv - Microbiology 2020Quote: Total RNA was isolated from groups of 5 HIOs per replicate with a total of 4 replicates per condition using the mirVana miRNA Isolation Kit (ThermoFisher). Cytosolic and mitochondrial ribosomal RNA was removed from samples using the Ribo-Zero Gold Kit according to manufacturer’s instructions (Illumina) ...
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bioRxiv - Immunology 2020Quote: ... Cells and virus were incubated for 4 hours at 37°C/5% CO2 and washed with PBS (Gibco, Waltham, MA) to remove excess virus ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were sonicated and aliquots containing 5 x 106 cells were separated on a NuPAGE bis-tris 4–12% gradient polyacrylamide gel (Invitrogen) under reducing conditions ...
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bioRxiv - Developmental Biology 2021Quote: ... The iPSCs were dissociated (DD0) after 4-5 days of maintenance using 0.25% trypsin / 1 mM EDTA (Thermo Fisher Scientific) at 37°C for 2 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... and 8 mM MgSO4 pH 7.0 (2x PHEM buffer) containing 1% Triton X100 for 5 min and then fixed in 4% (v/v) paraformaldehyde (ThermoFisher) in 2x PHEM buffer at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... was reconstituted by submerging the vial at 4°C over night and was diluted 1:5 in ice-cold knock-out DMEM (Cat.-No. 10829, Gibco) until material was evenly dispersed ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then separated by electrophoresis for a short amount of time (5 min) at 200 V on a NuPAGE 4-12% Bis-Tris gel in 1X MES/SDS (Invitrogen) running buffer ...
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bioRxiv - Immunology 2022Quote: ... 5-7 × 106 cells were resuspended in 3 mL of FACS buffer (4% FBS in phosphate buffered saline (PBS, Gibco)) and sorted at the University of Massachusetts Amherst Flow Cytometry Core Facility using a BD FACSAria Fusion (Becton Dickinson) ...
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bioRxiv - Neuroscience 2022Quote: ... Brain sections were incubated in blocking solution (0.2% Triton X-100 in PBS with 5% normal goat serum) for 1 hr at 4°C and then with a primary antibody anti-GFP (Invitrogen, catalog # G10362 ...
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bioRxiv - Microbiology 2022Quote: ... The RNA was precipitated with ice-cold isopropanol and 3 M sodium acetate (pH 5) at 4°C before RNA pellets were suspended in nuclease-free H2O and treated with DNase I (Ambion) for 30 min at 37°C ...