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Citations for Thermo Fisher :
1451 - 1500 of 10000+ citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... were then mixed 1:1,000 with 5 mL of MMB agar + Tetracycline and poured into 4-well Nunc Rectangular Dishes (ThermoFisher). Dishes were dried at room temperature for 1 hour ...
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bioRxiv - Genetics 2023Quote: ... mice were overdosed with inhaled isoflurane (>5%) and transcardially perfused with 4°C Ca2+/Mg2+-free 1X HBSS (Invitrogen). Bilateral T11-L2 DRGs ...
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bioRxiv - Neuroscience 2024Quote: ... 0.05% Tween-20) overnight at 4 °C and with secondary antibodies diluted in 5% nonfat milk in TBST (ThermoFisher, A16078 [goat anti- mouse HRP ...
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bioRxiv - Molecular Biology 2023Quote: ... SDS-PAGE gels were loaded with 5-15 μL of lysate per lane (NuPAGE 4-12% Bis-Tris; Invitrogen). Lysates were diluted with additional 1x NuPAGE LDS buffer if necessary ...
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bioRxiv - Microbiology 2023Quote: ... 5′-labelled Aar RNA (4 nM) was incubated for 1 h at 37°C with 1× structure buffer (Ambion), 1 µg yeast RNA and putative mRNA interaction partners at the indicated concentrations (0 nM ...
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bioRxiv - Biochemistry 2023Quote: ... heated for 5 minutes at 95°C and loaded on 4-12% Bis-Tris NuPage gels (Thermo Fisher Scientific). After electrophoretic separation ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were clarified by centrifugation (18,253 xg, 5 min, 4°C) and protein concentrations determined by absorbance on a NanoDrop instrument (ThermoFisher). Equal amounts of lysate were mixed with 4x loading dye (2% SDS ...
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bioRxiv - Plant Biology 2022Quote: ... pH 5.5 and 100 μM 3′,5′-dimethoxy-4′-hydroxyacetophenone (acetosyringone) (115540050; Acros Organics, dissolved in dimethyl sulfoxide (DMSO) (D8418 ...
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bioRxiv - Developmental Biology 2023Quote: ... transgenic hg13 larvae (mmachchg13/hg13 Tg(col2a1a:EGFP)) were staged and fixed at 5 DPF with 4% paraformaldehyde (Fisher Scientific). Larvae were dissected for genotyping and unbiased imaging was performed on the remaining head tissue ...
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bioRxiv - Developmental Biology 2023Quote: ... Larvae were maintained in embryo media at 28.5°C until 5 DPF and fixed in 4% paraformaldehyde (Fisher Scientific) at room temperature for 1 hour and stored in 1x PBS (Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were subsequently centrifuged for 5 minutes at 200 x g and resuspended in 4% paraformaldehyde (Thermo Scientific Chemicals) for 30 minutes at 22°C ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were rinsed with PBS (4 x 5 min) and mounted on microscope slides with ProLong Gold (ThermoFisher Scientific). Slices were kept in the fridge for at least two weeks before confocal imaging ...
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bioRxiv - Physiology 2024Quote: Platelet intracellular Ca2+ was measured as described previously using 5 µM Fluo-4 fluorescence dye (Invitrogen, Waltham, Massachusetts, USA).20 1 x 105 washed platelets were labeled with Fluo-4 for 30 min at room temperature (RT) ...
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bioRxiv - Cancer Biology 2024Quote: Passaging of ROBO1+ sorted Rounded/CBC cultures every 4-5 days is best done by incubating cells with 0.25% trypsin-EDTA (Gibco) for 1-2 min at RT/37°C ...
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bioRxiv - Molecular Biology 2024Quote: 786-O cells (5 × 104) were seeded in 4-well Nunc Lab-Tek II chamber slides (Thermo Fisher Scientific) and cultured for 2 days ...
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bioRxiv - Molecular Biology 2024Quote: ... 786-O cells (5 × 104) were seeded in 4-well Nunc Lab-Tek II chamber slides (Thermo Fisher Scientific) and cultured for 2 days ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained in FACS buffer (PBS supplemented with 2% FBS and 5 mM EDTA) for 30 min at 4°C using the following antibodies: eFluor450 anti-mouse CD4 (GK1.5; Invitrogen), BV605 anti-mouse CD8 (53-6.7 ...
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bioRxiv - Cell Biology 2024Quote: ... and the supernatant was replaced with 5 mM glucose-containing RPMI 1640 with Di-4-ANEPPDHQ (Thermo Fisher Scientific) at 2 µg/mL ...
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bioRxiv - Immunology 2024Quote: ... heated to 70°C for 5 min and ran on a 4-12% Nupage precast gel (Thermo Fisher Scientific). Protein were then detected using InstantBlue™ Coomassie Stain (Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... BODIPYTM-C12 500/510-C1 (4,4-difluoro-5-methyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid; Invitrogen) or BODIPYTM-C12 558/568 (4,4-difluoro-5-(2-thienyl)-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid ...
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bioRxiv - Biophysics 2024Quote: ... 5 μl of protein lysate was separated by gel electrophoresis on a NuPAGE 4-12% Bis-Tris Gel (ThermoFisher) and transferred by semi-dry transfer in NuPAGE transfer buffer (ThermoFisher ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... centrifuged at 500xg for 5 minutes at 4°C using a swinging bucket rotor (Sorvall Legend RT, Thermo Fisher), and then pellets were washed with an additional 1 mL cold lysis buffer and incubated on ice for an additional 5 minutes.
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bioRxiv - Biochemistry 2024Quote: ... RNAs were labeled at room temperature for 4 h in the dark by adding 5’-(iodoacetamido)fluorescein (IAF) (ThermoFisher) dissolved in DMSO to a final concentration of 7.5 mM ...
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bioRxiv - Cancer Biology 2024Quote: ... then denatured at 95°C for 5 minutes and loaded on 4-12% NuPAGE™ Bis-Tris gels (Invitrogen). Samples were transferred to a nitrocellulose membrane using a semi-wet transfer ...
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bioRxiv - Cell Biology 2024Quote: ... was boiled for 5 minutes at 95°C and separated on a 4-12% Bis-Tris gel (NP0321BOX, Invitrogen) in MES running buffer (NP0002 ...
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bioRxiv - Neuroscience 2021Quote: ... Neurons were incubated with 5 μM Fura-2 AM and 0.04% Pluronic F-127 (Thermo Fisher Scientific) for 45-60 min at 37 °C in standard extracellular solution ...
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bioRxiv - Molecular Biology 2020Quote: ... Acclaim PepMap 100 nanoViper C18 column (Thermo Scientific, 100 µm ID×2 cm, 5 μm, 100 Å) was used as pre-column for sample loading ...
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bioRxiv - Biochemistry 2021Quote: ... 2′-(or-3′)-O-(N-Methylanthraniloyl) adenosine 5′-triphosphate (mant-ATP, Thermo-Fisher Scientific, Waltham, MA, USA) was serially diluted to concentrations of 5 -15 μM in assay buffer and added to the myosin at a final concentration of 1X -1.2X the final concentration of myosin ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 μl of RNase A/T1 mix (2 μg/μl, 5 U/μl, Thermo Scientific, cat#EN0551) was added to 400 μl of the cleared lysate and incubated for 20 min at room temperature ...
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bioRxiv - Immunology 2022Quote: ... blocked for 2 hours at room temperature with 1X PBS supplemented with 5% Fetal Calf Serum (Invitrogen) and 0.25% Tween-20 (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... 5 x 106 S2 cells were seeded in 2 mL Schneider’s Drosophila media (Thermo Fisher, Waltham, Massachusetts) supplemented with 10% heat-inactivated fetal bovine serum in 9.6 cm2 wells of a tissue culture plate and incubated at 26 °C ...
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bioRxiv - Physiology 2020Quote: ... The membrane was probed with primary antibodies for α-Tubulin (ThermoFisher 322588, clone B-5-1-2), acetylated (Sigma T7451 ...
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bioRxiv - Immunology 2020Quote: Cells were harvested from the peritoneum by lavage with 5 ml cold PBS + EDTA (2 mM, Gibco). After washing ...
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bioRxiv - Genetics 2020Quote: ... cells were passaged into differentiation media supplemented with 5-Ethynyl-2’-deoxyuridine (Life Technologies, A10044, 10 µM). On day 6 of differentiation ...
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bioRxiv - Molecular Biology 2021Quote: 5-ethynyl-2’-deoxyuridine (EdU)-incorporation assay has been performed using a commercially available kit (Invitrogen, C10340). Animals were injected intraperitoneally with 60 µl of EdU (0.5 mg/ml in 50% DMSO and 50% PBS solution ...
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bioRxiv - Developmental Biology 2021Quote: ... received two intraperitoneal (i.p.) injections of 5-ethynyl-2’-deoxyuridine (EdU) (Thermo Fisher Scientific, Cat. No.: A10044) 1mg each and 4 hours apart ...
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bioRxiv - Neuroscience 2023Quote: Pregnant dams were injected intraperitoneally at e12.5 with a solution containing 5-Ethynyl-2’-deoxyuridine (EdU, ThermoFisher) and embryos were collected 2h after ...
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bioRxiv - Neuroscience 2023Quote: ... The BV-2 cells were cultured in DMEM supplemented with 5% heat-inactivated fetal bovine serum (Gibco) and 1% penicillin-streptomycin ...
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bioRxiv - Cell Biology 2023Quote: ... and the sample was washed with 1X PBS for 2 x 5 min using a Pipetman (Gibco), then blocked using the blocking solution for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... at 150 × 106 cells/dish for 2 h at 37°C/5% CO2 in RPMI-1640 (Gibco) to separate adherent cells ...
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bioRxiv - Biochemistry 2024Quote: ... 5 μL of PCR products was run on a 2% E-gel EX Agarose Gel (ThermoFisher #G401002) for 10 minutes according to the settings programmed into the gel system ...
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bioRxiv - Developmental Biology 2024Quote: ... was prepared and added to 426.5 μL 2:1 (vol:vol) 0.1x Tris (Fisher Scientific, cat.# BP152-5)-ethylenediaminetetraacetic acid (EDTA ...
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bioRxiv - Cell Biology 2024Quote: Vacuolar pH alterations were detected using the 5-(and-6)-carboxy-2′,7′-dichlorofluorescein diacetate (CDCFDA, Invitrogen) probe ...
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bioRxiv - Evolutionary Biology 2023Quote: We conducted all experiments using SM/5 media (Loomis and Sussman 1966) (2 g glucose (Fisher Scientific), 2 g BactoPeptone (Oxoid) ...
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bioRxiv - Cell Biology 2022Quote: ... then transfected for 5 hours with indicated plasmids in 2 mL Opti-MEM (Invitrogen, Cat. #31985-088) using Lipofectamine 3000 transfection kit (Thermo Fisher ...
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bioRxiv - Developmental Biology 2024Quote: Dams were injected IP with 30 mg/kg body weight of 5-ethynyl-2’-deoxyuridine (EdU; Invitrogen) 12 hours prior to harvesting at E18.5 ...
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bioRxiv - Neuroscience 2024Quote: ... mixed retinal cultures were loaded with 5 µM Fura-2 AM (acetoxymethyl ester, #F1221; Thermo Fisher Scientific) with 0.01% pluronic F-127 (#P3000MP ...
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bioRxiv - Bioengineering 2024Quote: ... 2 µL mL-1 egg lecithin (5 mM in EtOH) and 1x chemical defined lipid concentrate (Gibco). After incubation for 2 days ...
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bioRxiv - Cancer Biology 2024Quote: ... permeabilized in 0.3% Triton X-100 for 5 min and blocked in 2% blocking solution (Thermo fisher). Cells were incubated overnight with the primary antibodies washed in PBS and secondary antibodies were applied for 1 hr before counter-staining with DAPI ...
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bioRxiv - Synthetic Biology 2024Quote: EVs (2×109 particles in 15 µL) were mixed with a 5 µL sample buffer (NP0007, Invitrogen) and heated at 70 ℃ for 10 min ...