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Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... pH-7.4) and then loaded with the ratiometric Ca2+ indicator Fura-2 AM (4 µM) (Acetoxymethylester, Invitrogen) along with 0.002% Pluronic F-127 for 30 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... 2021 Total RNA was extracted from 2-4 day old female ovaries using the MirVana kit (Ambion). rRNA was depleted using antisense rRNA oligo hybridization with subsequent RNase H digestion ...
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bioRxiv - Cell Biology 2023Quote: ... DAPI (4’,6-Diamidino-2-henylindole, dihydrochloride) was obtained from Invitrogen (Catalog: D1306, CAS:28718-90-3).
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bioRxiv - Biochemistry 2023Quote: ... The slides were then incubated with 1 ug/ml of 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen) in 1xPBS for 20 min at RT ...
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bioRxiv - Neuroscience 2023Quote: ... 2 dpf zebrafish larvae were dechorionated and fixed with 4% formaldehyde methanol-free (Pierce™ Thermofisher, #28906) in BT buffer (1.0g sucrose ...
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bioRxiv - Plant Biology 2023Quote: ... 250 ng/ml 4′,6-diamidino-2′-phenylindole dihydrochloride (DAPI) or 500 nM SYTOX Orange (Life Technologies, Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... After another set of gentle washes and 4′,6-diamidino-2-phenylindole (DAPI) nuclear staining (Thermo Scientific, R37606 ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were visualized with 4′,6-diamidino-2-phenylindole (DAPI) in SlowFade Diamond antifade mounting medium (ThermoFisher). Primary antibodies were incubated with tissues overnight at 4°C in 1% normal donkey serum ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei staining was performed with 4’,6 diamidino-2-phenylindole dihydrochloride (DAPI, Molecular Probes, Eugene, OR, USA) at 300 nM in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... The sections were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; 0.02 mg/mL, ThermoFisher Scientific, USA), mounted and cover-slipped before being imaged with the Zeiss Axio Imager.Z1 Microscope ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were stained with DAPI (4’,6-diamidino-2-phenylindole, dihydrochloride) NucBlue (Cat. #: R37606; Thermo Fisher Scientific). Slides were mounted using Aqua-Poly/Mount (Cat ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 2 hour incubation at 4℃ with 50 µL of magnetic Dynabeads Protein G (Invitrogen, #10004D). Beads were washed with 1x TBS Tween-20 washing buffer (Thermo Scientific ...
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bioRxiv - Microbiology 2024Quote: ... GECs (8 x 104 cells) were seeded in 4-well chamber slides (Fisher Scientific# 12-565-2) and pre-treated with 2 mM Glu-Glu for 1 h prior to infection with wild-type or ΔgdhA strains of P ...
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bioRxiv - Genomics 2024Quote: ... Sections were stained with DAPI (4 ',6' -diamidino-2-phenylindole) and mounted in Prolong Gold (Life Technologies). Imaging was performed on a Nikon Eclipse Ti Confocal at 20X objective and processed using Nikon Elements-AR ...
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bioRxiv - Microbiology 2024Quote: ... hMDM nuclei were stained with 50 ng/ml 4’,6-diamidino-2- phenylindole (DAPI) (Invitrogen Cat. # D1306) for 7 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclear counterstaining was performed using 300 nM DAPI (4’,6-Diamidino-2-Phenylindole, dihydrochloride) (Thermo Fisher # D1306).
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bioRxiv - Neuroscience 2024Quote: ... Nuclear counterstain was performed using 300 nM DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride) (Thermo Fisher # D1306).
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bioRxiv - Biophysics 2024Quote: ... 4-(2-hydroxyethyl)-1-piparazineethanesulphonic acid (HEPES) and tris(hydroxymethyl)aminomethane (Tris) were purchased from Fisher Scientific.
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bioRxiv - Synthetic Biology 2024Quote: ... The cell nuclei was labelled by adding 4′,6-diamidino-2-phenylindole (DAPI, D1306, Thermo Fisher Scientific) for 15 min ...
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bioRxiv - Biophysics 2024Quote: ... Samples were washed in PBS and stained with 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Scientific, USA) for 5 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... Sections were counterstained with DAPI (4′,6-diamidino-2-phenylindole) and mounted with ClearMountTM mounting solution (Invitrogen).
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bioRxiv - Cancer Biology 2024Quote: ... Gibco 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer (1 M) was obtained from Invitrogen (Carlsbad, CA) and sterile filtered before use ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by staining with 4’,6-diamidino-2-phenylindole (DAPI) nuclear dye (Thermo Fisher Scientific, Cat# D3571) for 5 min ...
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bioRxiv - Developmental Biology 2024Quote: ... batches of 4 embryos were digested in 40µl of a solution of 2:1 0.25% Trypsin (Gibco): Accutase (StemCell Technologies ...
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bioRxiv - Biochemistry 2024Quote: ... 150 mM KCl, 1 mM EDTA ethylenediaminetetraacetic acid [EDTA], 2 mM MgCl2, 0.5 mM ATP, 0.02% Brij-35 [ThermoFisher Scientific], 5 mM 2-ME, pH 8.0) supplemented with 50 U ml-1 Benzonase (Merck-Millipore) ...
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bioRxiv - Neuroscience 2020Quote: ... Fluo-4 (Fluo 4 AM/Fluo 4 direct, Life Technologies) was used according to the manufacturer’s instructions and a previously published protocol (54) ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatant (1 ml at ~4-5 mg/ml) was incubated with 50 μl of superparamagnetic Dynabeads Streptavidin C1 (Invitrogen) and rotated overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were washed for 4 x 5 min in PBS and mounted in Prolong Gold with DAPI stain (Invitrogen). Imaging was performed on an Axio Observer microscope (Zeiss ...
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bioRxiv - Genomics 2020Quote: ... 5 mM MgCl2, 1 mM EGTA, 0.5 mM DTT, 0.05% Tween-20, 4 U/mL SUPERase-In [Thermo Fisher] ...
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bioRxiv - Microbiology 2021Quote: ... Harvested cells were normalized to the lowest OD-600 of the collected cells and pelleted by centrifugation at 20000XG for 2 mins and resuspended in 75 µL lysis buffer (10 mM Tris-HCl, pH 7.0, 4% SDS, 5 U DNaseI (ThermoFisher), 0.2 tablets of PhosStop (Roche)) ...
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bioRxiv - Neuroscience 2020Quote: ... at 95 °C for 5-10 min and loaded on a gradient 4-12% NuPAGE Bis-Tris gels (Invitrogen). After electrophoresis at 200 V ...
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bioRxiv - Microbiology 2020Quote: ... Cell envelopes were stained by adding 5 µL of 1 mg/mL FM™ 4-64FX (Thermo Fisher Scientific) and non-viable cells stained with 1 µL of the LIVE/DEAD™ cell dye (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Remnant villi were removed by shaking tissue pieces at 4°C in an initial 5 minute incubation PBS with 10 mM RNase-free EDTA pH 8.0 (Invitrogen). Crypts were collected and filtered through 70 µm cell strainers in subsequent fractions ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated with primary antibody O/N at 4°C (5% FBS, 0.5% Tx, 0.2% gelatine in PBS; GFP (1:500, ThermoFisher Scientific). Slices were then repeatedly washed with PBS-0.5% Tx ...
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bioRxiv - Biochemistry 2022Quote: ... and heated at 95°C for 5 min before loading onto a NuPAGE Bis-Tris 4-12% gel (Invitrogen). Immunoblotting was performed using a Mini Trans-Blot cell (BioRad ...
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bioRxiv - Neuroscience 2020Quote: ... brains were transferred through 4 wells containing a blocking solution (5% Goat Serum in PBT; Life Technologies #16210-064), and sitting in the last well for 30 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... denaturated 5 min at 95°C and finally loaded on NuPAGE 4-12% Bis-Tris protein gels (Life Technologies) and electro-transferred onto nitrocellulose membranes ...
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bioRxiv - Molecular Biology 2021Quote: ... SDS-PAGE gels were loaded with 5 - 15 μL of lysate per lane (NuPAGE 4-12% Bis-Tris; Invitrogen). Lysates were diluted with additional 1x NuPAGE LDS buffer if necessary ...
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bioRxiv - Microbiology 2020Quote: ... and the wells were overlaid with 4 ml/well DMEM with 5% FBS and 0.7% agarose (Invitrogen, 16500-500). At 6 d.p.i. ...
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bioRxiv - Bioengineering 2020Quote: ... 2.5 μM of CellTracker™ Orange 5-(and-6)-(((4-chloromethyl)benzoyl)amino)tetramethyl-rhodamine CMTMR (Molecular Probes, C2927) was used to label iNeuron cells ...
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bioRxiv - Genomics 2021Quote: ... we extracted total RNA from rosette leaves of 4-5-week-old plants using Trizol (Invitrogen, cat. No. 15596026) according to manufacturer’s manual ...
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bioRxiv - Genomics 2021Quote: ... centrifuged at 500xg for 5 minutes at 4°C using a swinging bucket rotor (Sorvall Legend RT, Thermo Fisher), and then pellets were washed with an additional 1 mL cold lysis buffer and incubated on ice for an additional 5 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... which were boiled for 5 min at 95°C and then separated on 4-12% polyacrylamide gradient gels (Invitrogen). After blotting ...
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bioRxiv - Molecular Biology 2021Quote: ... A total of 5 μg protein was run on a NuPage™ 4-12% Bis-Tris Protein Gel (Invitrogen) using MOPS buffer according to manufacturer instructions and subsequently transferred to a nitrocellulose membrane according to the NuPage™ manufacturer's protocol (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... lysed in 1X Novex buffer and 5-7.5×105 cells equivalents were resolved on 4-12% NuPAGE gel (Invitrogen) in MOPS buffer ...
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bioRxiv - Plant Biology 2022Quote: ... pH 5.5 and 100 μM 3′,5′-dimethoxy-4′-hydroxyacetophenone (acetosyringone) (115540050; Acros Organics, dissolved in dimethyl sulfoxide (DMSO) (D8418 ...
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bioRxiv - Neuroscience 2024Quote: ... 0.05% Tween-20) overnight at 4 °C and with secondary antibodies diluted in 5% nonfat milk in TBST (ThermoFisher, A16078 [goat anti- mouse HRP ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were heated to 80°C for 5 min and electrophoresed on 4-12% NuPAGE Bis-Tris gels (ThermoFisher). Wet transfer was to a nitrocellulose membrane (0.45 uM pore ...
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bioRxiv - Cell Biology 2024Quote: ... and aliquots containing 5 × 106 cells were separated on a NuPAGE bis-tris 4–12% gradient polyacrylamide gel (Invitrogen). The sample lane was divided into eight slices that were excised from the Coomassie-stained gel ...
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bioRxiv - Microbiology 2024Quote: ... were then mixed 1:1,000 with 5 mL of MMB agar + Tetracycline and poured into 4-well Nunc Rectangular Dishes (ThermoFisher). Dishes were dried at room temperature for 1 hour ...