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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for Respiratory syncytial virus RSV fusion protein exodomain human heterodimeric IgG1 Fc tag recombinant antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... were cultured in in K-SFM + Human recombinant Epidermal Growth Factor (rEGF) and Bovine Pituitary Extract (BPE) supplemented with 1% penicillin and streptomycin (all from Thermo Fisher Scientific). PCa cell line PC-3 (CRL-1435) ...
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bioRxiv - Cell Biology 2024Quote: ... DD5A)29 and three controls (Ctl1B, Ctl7C, Ctl8F)47 were cultured on human recombinant laminin LN521 (Biolamina) in Essential-8TM medium (Thermo Fisher Scientific) and passaged using Trypl Express (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: The HA pull down was performed using 50μl of the Immobilized Recombinant Protein G Resin (Generon) and specific antibodies against HA (Invitrogen). In order to perform the pull down ...
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bioRxiv - Microbiology 2022Quote: Recombinant proteins were produced in transiently transfected suspension culture of FreeStyle 293 cells (Thermo Fisher Scientific, Waltham, MA, USA), as previously described 66 ...
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bioRxiv - Biochemistry 2021Quote: ... The recombinant proteins were affinity purified by overnight binding to HisPur Cobalt Resin (Thermo Fisher Scientific, U. S. A.) in buffer A (50 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Immunology 2020Quote: ... The vector was linearized with BglII and transformation of yeast (Pichia pastoris) and induction of recombinant protein expression was carried out according to the manufacturer’s instructions (Invitrogen). Recombinant HELMCC protein was purified from yeast culture supernatants by ionexchange chromatography using Ni2+ affinity chromatography (Amersham Biosciences) ...
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bioRxiv - Microbiology 2020Quote: ... Purified soluble recombinant proteins were tested for bacterial endotoxins using the Limulus Amebocyte Lysate (LAL) assay kit (Invitrogen, USA).
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bioRxiv - Biochemistry 2022Quote: ... Pierce™ recombinant protein A/G and clear round-bottom immuno nonsterile 96-well plates were obtained from ThermoFisher Scientific ...
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bioRxiv - Biophysics 2019Quote: ... Cells were lysed by sonication and recombinant proteins purified by Ni2+ affinity chromatography in Ni-NTA agarose (Thermo Fisher) using 50 mM TrisHCl/500 mM NaCl/10 mM imidazole [ph 8.0] as binding buffer ...
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bioRxiv - Microbiology 2022Quote: recombinant biotinylated sE-cvD protein was expressed at 28°C using an ExpiFectamine 293 transfection kit (Thermo Fisher Scientific). Cell supernatant was harvested and dialyzed ...
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bioRxiv - Molecular Biology 2024Quote: ... Purity of recombinant proteins was verified by SDS-PAGE with staining by GelCode Blue stain reagent (Thermo Fisher scientific).
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bioRxiv - Neuroscience 2024Quote: ... A polyclonal antibody to Drosophila tau was prepared in rabbits immunized with full length recombinant tau protein (Thermo Fisher) and was used at 1:5,000,000 for western blotting ...
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bioRxiv - Microbiology 2024Quote: Recombinant soluble spike and RBD proteins from Wuhan-1 and BA.1 SARS-CoV-2 strains were expressed as described 58,59 Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant proteins were purified by immobilized metal affinity chromatography (IMAC) as previously described30 using HisPur cobalt resin (Thermo Scientific). Before elution ...
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bioRxiv - Cell Biology 2023Quote: ... the lysates were incubated in shake tubes over 1 h at 4°C with recombinant protein G–Sepharose (Invitrogen). The Sepharose was washed with RIPA lysis buffer and eluates were obtained through an addition of Laemmli Buffer.
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bioRxiv - Biochemistry 2023Quote: ... The coding sequences of the recombinant proteins were then inserted into the BamHI and EcoRI sites of the pcDNA3.1(+) vector (Invitrogen), and all clones were verified through DNA sequencing.
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant lentivirus used to infect SW620 cells for protein expression was generated by the ViraPower Lentiviral Expression System (Invitrogen) according to the manufacturer’s instruction.
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bioRxiv - Pharmacology and Toxicology 2021Quote: An Orbitrap Fusion (Thermo Fisher Scientific) and RSLC Ultimate 3000 nano-UPLC (Dionex ...
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bioRxiv - Neuroscience 2022Quote: ... an Orbitrap Fusion Lumos (Thermo Scientific) was used ...
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bioRxiv - Neuroscience 2023Quote: ... An Orbitrap Fusion (Thermo Fisher Scientific) was used to acquire all the MS spectral data ...
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bioRxiv - Neuroscience 2023Quote: ... An Orbitrap Fusion (Thermo Fisher Scientific) was used to acquire all the MS spectral data ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: An Orbitrap Fusion (Thermo Fisher Scientific) and RSLC Ultimate 3000 nano-UPLC (Dionex ...
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bioRxiv - Developmental Biology 2024Quote: ... Orbitrap Fusion Lumos Tribrid (Thermo Fisher) mass spectrometer was operated in the data-dependent mode ...
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bioRxiv - Microbiology 2020Quote: ... Desalted peptides were labeled with Tandem Mass Tags (TMT) using TMTPro-16plex isobaric tags (ThermoFisher Scientific; #A44520) as per manufacturer’s instructions ...
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Variation of wine preference amongst consumers is influenced by the composition of salivary proteinsbioRxiv - Biochemistry 2023Quote: Tandem mass tag (TMT) labelling was performed using the TMT10plexTM Mass Tag Labelling Kit (Thermo Fisher Scientific) with minor modifications from the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... the receptor Fc-tagged ectodomains present in the conditioned media were captured on protein A-coated 384-plates (Thermo Scientific), and stored at 4 °C until use ...
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bioRxiv - Immunology 2020Quote: ... washed 2x with PBS 0.5% FCS and then stained with the Click-it Plus OPP Protein Synthesis Kit Alexa Fluor 647 (C10458, Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... Stop reaction was added in each well and protein was measured at 450 nm in a Multiskan FC (Thermo Scientific).
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bioRxiv - Biochemistry 2023Quote: ... All FCS measurements were carried out at ∼20 nM fluorescently labeled protein at 20°C in Nunc chambered coverslips (ThermoFisher). The chambers are pre-incubated with poly-lysine conjugated polyethylene glycol (PLL-PEG ...
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bioRxiv - Molecular Biology 2020Quote: ... Precleared nuclear protein lysates (500 μg) were incubated with 20 μl of anti-c-MYC tag antibody coupled to magnetic beads (Thermo Scientific). After two hours ...
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bioRxiv - Cell Biology 2021Quote: ... and 100 μg of the total proteins from each cell line were labelled with 6-plex isobaric tandem mass tag (TMT6) reagents (Thermo Scientific) following the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 µg of an HA-tag antibody (MBL, M132-3) was mixed with 20 µL of Dynabeads Protein G (Invitrogen, #10004D) and subsequently added to extracts ...
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bioRxiv - Molecular Biology 2022Quote: Insect or bacterially expressed proteins were purified by His-tag purification using hiTRAP FF columns (GE) or HisPur Resin (Thermo Scientific), followed by His tag removal by Tobacco Etch Virus (TEV ...
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bioRxiv - Microbiology 2020Quote: ... Immunoprecipitation of TgTRPPL-2-smHA protein was performed using the Pierce HA Tag/Co-IP Kit (Thermo Fisher Scientific, Waltham, MA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... recombinant SARS-CoV-2 Spike RBD protein (RBD, His Tag) was conjugated with EZ-LinkTM Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Microbiology 2023Quote: Changes in protein secretion were relative quantified with the isobaric standard tandem tag (TMT) 10 plex labelling kit (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: Capillary-liquid chromatography-nanospray tandem mass spectrometry of protein identification was performed on an orbitrap Fusion mass spectrometer equipped with an EASY-Spray sources (Thermo Fisher). Sequence information from the MS/MS data was processed by converting the .raw files into a merged file (.mgf ...
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bioRxiv - Plant Biology 2022Quote: Changes in the protein abundancies was assessed using trypsin digested samples analysed using a Fusion Lumos Tribrid Orbitrap mass spectrometer (Thermo Scientific) in a data independent acquisition (DIA ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation for 3 hr at 4°C with 250 μg of GST-fusion protein coupled to glutathione-agarose (Thermo Scientific). Beads were then washed four times (pull down buffer containing 0.1% Triton X-100 ...
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bioRxiv - Neuroscience 2019Quote: Full-length Eya1 wild type and mutant D328A protein in a GST-fusion format were generated using the Bac-to-Bac Baculovirus Expression system (ThermoFisher Scientific) and expressed in Sf9 insect cells ...
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bioRxiv - Developmental Biology 2023Quote: ... 5’-capped mRNA encoding the HpCTCF-GFP fusion protein was synthesized using the mMESSAGE mMACHINE™ T7 Trinscription Kit (Ambion, USA) and purified by phenol/chloroform extraction and isopropanol precipitation ...
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bioRxiv - Neuroscience 2023Quote: ... 200 µg of the proteins was incubated with equivalent amount of GST-α synuclein fusion protein at 4°C overnight in the present of Ni-NTA beads (Invitrogen). The pull-downs were washed 4 times with lysis buffer and boiled in 2X Laemmli sample buffer for five minutes.
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bioRxiv - Bioengineering 2023Quote: ... mCXCL10-LPETG SUMO fusion proteins were purified by Ni-NTA pulldown and then treated overnight with Endotoxin Removal columns (Thermofisher, 88274). Cleavage of mCXCL10-LPETG from the greater SUMO fusion proteins was carried out via ULP1 digestion (Thermofisher ...
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bioRxiv - Immunology 2022Quote: ... All fusion proteins were expressed in Expi-293F cells in 250-280 ml culture using the ExpiFectamine 293 Transfection Kit (Life Technologies) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysates were cleared by centrifugation at 20,000 rpm for 45 min and the His-SUMO fusion proteins were enriched on Ni-NTA beads (Thermo Fisher Scientific). The protein was eluted using a buffer containing 25 mM Tris (pH 8.0) ...
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bioRxiv - Developmental Biology 2023Quote: ... we constructed a fusion protein with Cte-BMPR1ΔK and mVenus using pSPE3-RfA-Venus (43) and LR Clonase II (Invitrogen 11791-020), and the final expression vector ...
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bioRxiv - Cell Biology 2020Quote: The concentration of IFNλ1/IL-29 was measured in basolateral medium from RSV BT2a- or mock-infected cultures at 96 hpi by ELISA (Thermo Fisher Scientific; BMS2049). The manufacturer’s instructions were followed.
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bioRxiv - Microbiology 2023Quote: ... The following custom TaqMan gene expression assay IDs were used to assess the expression of RSV N and SARS-CoV-2 N genes: ARU66XH and APNKYWD (Applied Biosystems, CA, USA). A no template control was included in each run ...
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bioRxiv - Molecular Biology 2022Quote: ... The relative levels of the assembled respiratory complexes I-V were assessed by western blot with following antibodies: anti-ND1(Thermo Fisher, 19703-1-AP), anti-SDHA (Cell signaling ...
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bioRxiv - Microbiology 2021Quote: ... For samples that were mixed with protein (BSA, human CAR, mouse CAR [Fisher Scientific]) prior to attachment ...