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Citations for Thermo Fisher :
1801 - 1850 of 10000+ citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Labelled peptides were resolved using an easyLC1200 nano-chromatograph (Thermo Scientific), peptides were loaded on a 25 cm x 75 μm ID column packed in house with Reprosil C18 silica particles (1.9 μm diameter ...
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bioRxiv - Neuroscience 2023Quote: ... and calcitonin gene-related peptide (CGRP) (ThermoFisher, cat # PA185250, 1:500) overnight at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... peptides were washed on a PepMap Neo C18 trap column (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were separated using an Easy nLC 1200 (Thermo Fisher Scientific) equipped with a nano-ESI spray column (NTCC-360 ...
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bioRxiv - Immunology 2024Quote: ... Peptides were analysed on a Q-Exactive-HF-X (Thermo Scientific) mass spectrometer coupled with a Dionex Ultimate 3000 RS (Thermo Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... the peptide concentration was measured using the BCA assay (Thermo Scientific). For LC-MS/MS measurements 800 ng of peptides were analyzed on a Q- Exactive Plus mass spectrometer (Thermo Scientific ...
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bioRxiv - Physiology 2024Quote: ... Peptides were loaded on a C18 reversed-phase precolumn (Thermo Scientific) followed by separation on a with 2.4 µm Reprosil C18 resin (Dr ...
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bioRxiv - Cell Biology 2024Quote: ... Peptides were separated by an Easy-nLC 1200 (Thermo Fisher Scientific) coupled to an Exploris 480 mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2024Quote: ... Peptide amounts were quantified using the fluorometric CBQCA assay (Thermo Scientific).
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bioRxiv - Cell Biology 2024Quote: ... Peptides were labelled with the TMTpro-16plex reagents (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... tryptic peptides were quantified by fluorimetry using QuBit (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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Spirolactone, an unprecedented antifungal β-lactone spiroketal macrolide from Streptomyces iranensisbioRxiv - Biochemistry 2024Quote: Peptides were loaded onto a 2cm C18 trap column (ThermoFisher 164946), connected in-line to a 15cm C18 reverse-phase analytical column (Thermo EasySpray ES904 ...
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bioRxiv - Cell Biology 2024Quote: ... peptides were labeled with TMTPro 16plex reagents (ThermoFisher Scientific, Cat. A44520). 6 mM of each label (except 127C ...
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bioRxiv - Cell Biology 2024Quote: Peptides were loaded onto a 2cm C18 trap column (ThermoFisher 164946), connected in-line to a 15cm C18 reverse-phase analytical column (ThermoFisher EasySpray ES904) ...
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bioRxiv - Microbiology 2024Quote: The peptides were labelled with isobaric tags (TMTpro, Thermo Fisher Scientific) according to the supplier’s recommendations ...
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bioRxiv - Biophysics 2021Quote: ... The protein was incubated with SUMO protease at 4°C overnight before flowing over a Ni-NTA gravity column (ThermoFisher Scientific, 88222) to remove the cleaved 6xHis-TS-SUMO tag and SUMO Protease ...
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bioRxiv - Cell Biology 2020Quote: ... The extracts were incubated at 70 °C for 45 min and used for protein analysis or RNA isolation using Trizol LS (Thermo Fisher Scientific).
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bioRxiv - Immunology 2021Quote: P1 protein was analyzed at C-CAMP’s Mass spectrometry-based proteomics facility using Orbitrap Fusion Tribrid Mass Spectrometer (Thermo Fisher Scientific, USA) using optimized protocol for in-gel and in-solution trypsin digested product.
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bioRxiv - Genetics 2021Quote: ... aliquots of a custom-made rabbit anti-NHE6 antibody (C-terminal epitope: GDHELVIRGTRLVLPMDDSE, Covance #048) (Ouyang et al., 2013) were conjugated to Dynabeads Protein G (Thermo Fisher #10004D) at room temperature for 2 h according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Ago2 and IgG control and then incubated for 1 h at 4°C with Dynabeads™ Protein A or G (ThermoFisher Scientific). After incubation ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysates were then incubated overnight at 4°C with pan-flavivirus anti-E antibody and protein G Sepharose beads (Thermo Fisher Scientific). Beads were washed three times and eluted with 2× SDS loading buffer before Western blot analysis.
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bioRxiv - Molecular Biology 2020Quote: SEC fractions in loading buffer were heated to 95°C for 5 min and separated alongside a spectra multi-color protein ladder (Thermo Fisher 26634) on a 4-15% Tris-Glycine extended Stain-Free gel (Bio-Rad 5678085 ...
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Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responsesbioRxiv - Molecular Biology 2020Quote: ... from the pENTR clones to the BiFC vector pDEST-VYCE(R)GW carrying the C-terminal region of the Venus Fluorescent Protein (VFP) (Gehl et al., 2009) by Gateway® cloning (Invitrogen, California) from the pENTR clones to yield the constructs as listed in Supplementary table S2 ...
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bioRxiv - Developmental Biology 2019Quote: ... solubilized protein fractions were collected following centrifugation at maximum speed for 15 minutes at 4°C and protein concentrations were quantified by Bradford assay (Thermo Fisher Scientific). Equivalent amounts of protein per sample were boiled at 99°C for five minutes in 4X reducing Laemmli sample buffer and then centrifuged at maximum speed for five minutes at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... as per the manufacturer’s instructions and incubated with 10 μg primary antibodies overnight at 4°C followed by 90 minutes incubation with 35 μl protein A/G agarose plus (50% slurry) (ThermoFisher Scientific 20421). Immune pellets were washed 5 times in PBS containing 1% Triton-X100 and analyzed by SDS-PAGE followed by either immunoblotting or mass-spectrometry (LC/MS-MS) ...
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bioRxiv - Genetics 2019Quote: Coverslips were incubated for one hour at 37°C in 1mL of of 1:30 solution of Collagen I Rat Protein (Thermo Fisher Scientific) to 1X PBS ...
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bioRxiv - Neuroscience 2020Quote: ... 0.1% SDS and protease and phosphatase inhibitors) and stored at −20°C before sonication and quantification by a Pierce BCA Protein Assay Kit (Thermofisher Scientific, 23225). Protein lysates were heated for 5 min at 95°C in loading buffer (1% SDS ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were centrifuged for 5 min at 13 000 × g at 4°C and the concentration of protein was measured using a bicinchoninic acid assay (Thermo Fisher Scientific). Protein lysates were fractionated by SDS-PAGE and transferred onto PVDF membranes ...
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bioRxiv - Biochemistry 2020Quote: ... The chromatin was incubated with antibodies at 4 °C overnight and then added to 30 μl of protein G-Dyna beads (Thermo Fisher Scientific) for an additional 2 hours with constant rotation ...
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bioRxiv - Bioengineering 2020Quote: ... Samples were incubated at 70°C for 10 minutes and resolved by SDS-PAGE using NuPAGE 4-12% Bis-Tris Protein Gels (Thermo Fisher Scientific) followed by electroblotting onto Immun-Blot PVDF Membrane (Biorad ...
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bioRxiv - Biochemistry 2021Quote: ... This construct was expressed as a secreted protein by a stable Drosophila S2 cell line (30) and affinity purified using C-tag affinity resin (Thermo Fisher Scientific) followed by a size-exclusion chromatography polishing step in 20 mM Tris ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentrations were determined following centrifugation of the lysate at 16,000 x g at 4 °C for 20 minutes using the using a Pierce™ Coomassie (Bradford) Protein Assay Kit (23200, Thermo Scientific) with bovine serum albumin as the standard.
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bioRxiv - Microbiology 2020Quote: Recombinant biotinylated proteins (sE, sE-cvD and DIII) were expressed at 28°C using ExpiFectamine™ 293 Transfection Kit (Thermo Fisher Scientific). Cell supernatant was harvested and dialyzed ...
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bioRxiv - Developmental Biology 2021Quote: ... Fished-out FGFR3 protein was eluted and then denatured at 95°C for 10 minutes in NuPAGE LDS sample buffer (Life Technologies, NP0008) and β-mercaptoethanol 2.5%.
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bioRxiv - Molecular Biology 2020Quote: ... The isolated protein complex was eluted by incubating the beads overnight at 4°C with 200 units of TEV protease (Thermo Fisher Scientific) in 500 µl of TEV150 buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... overnight on an end-to-end rotator at 4°C followed by a 2.5-hr incubation with protein G Dynabeads (Thermo Fisher Scientific #10003D). Samples were then sequentially washed with TSE (0.1% SDS ...
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bioRxiv - Neuroscience 2022Quote: Proteins from MACsorted cells were extracted during 30 minutes at 4°C in RIPA buffer (ThermoFisher; 50 µL per million cells, 89901) supplemented with Halt™ Protease Inhibitor Cocktail (100X ...
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bioRxiv - Biochemistry 2022Quote: ... the supernatant was incubated at 4°C for 1 h with anti-BmAgo3 antibody23 conjugated to Dynabeads protein G (Thermo Fisher Scientific). The superparamagnetic beads were washed five times with wash buffer (30 mM HEPES-KOH ...
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bioRxiv - Immunology 2022Quote: Plasma was pooled and heat inactivated at 56°C for 1 hour then diluted in protein G binding buffer (Pierce ThermoFisher, catalog #54200) and passed over a column containing 1mL of protein A/G resin (Pierce ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... and Omicron RBD-his protein were coated overnight at 4 °C in a 96-well plate (MaxiSorp Nunc-immuno, Thermo Scientific, USA). Wells were blocked with 5% non-fat milk (Biofroxx ...
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bioRxiv - Microbiology 2022Quote: ... and Omicron RBD-his protein were coated overnight at 4 °C in a 96-well plate (MaxiSorp Nunc-immuno, Thermo Scientific, USA). Wells were blocked with 5% non-fat milk (Biofroxx ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were denatured for 1 minute at 70°C and RNA-protein complexes were resolved on NuPAGE 4-12% Bis-Tris Gels (Thermo Fisher, #WG1402A) at 180V for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... Recombinant S and RBD trimeric proteins secreted in cell culture supernatants were purified using the CaptureSelect™ C-tag Affinity Matrix (ThermoFisher Scientific). Concentration of purified recombinant Abs were determined by PierceTM BCA Protein Assay Kit (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysates were clarified by centrifugation at 100,000 g for 20 mins at 4°C and resulting supernatants were incubated with 50 μL of anti-HA magnetic beads or protein A magnetic beads (Thermo Fisher Scientific) for 1 h at 4°C with rotation (10 rpm) ...
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bioRxiv - Biochemistry 2021Quote: ... 0.03% NP40 supplemented with 50,100 or 200 mM KCl) and proteins were eluted by incubation in 1x LDS-sample buffer (Thermofisher, c/n NP0007) at 65°C for 10 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... dissolved in water was added to the protein solution and the specific proteolytic digestion proceeded for 16 h at 37 °C in a thermomixer (Thermo Fisher Scientific). Digestion was centrifuged at 10000 rpm during 1 min and 4 μL of the resultant mixture of tryptic peptides was mixed with 0.3 μL of 90% formic acid and it was loaded into a metal coated borosilicate nanocapilar for mass spectrometric analysis.
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bioRxiv - Systems Biology 2022Quote: ... and denatured at 70°C for 10 min prior to loading onto Novex NuPAGE 10% Bis-Tris Protein gels (Invitrogen, Cat.# NP0315BOX) and separated half way (15min at 200 constant voltage) ...
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bioRxiv - Immunology 2022Quote: ... Disrupted cells were then centrifuged at 15,800 x g for 30 min at 4°C and the resulting supernatant collected with an initial protein purification performed using the HisPur Ni-NTA Resin (Thermo Fisher Scientific) following manufacturer’s instructions for native protein confirmation and gravity flow columns ...
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bioRxiv - Neuroscience 2020Quote: ... Extracts were centrifuged at 15.000g for 10 min at 4°C and supernatants were collected and quantified with Pierce™ BCA Protein Assay Kit (Thermo Scientific) following manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Supernatants were then immunoprecipitated on a rotator for two hours at 4 °C with antibody pre-bound to 17 μl Protein A Dynabeads (Life Technologies 10001D). The following antibodies were used ...