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Citations for Thermo Fisher :
1501 - 1550 of 2521 citations for 7 Octenyldimethylmethoxysilane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... reduced whole cell lysates were electrophoresed on a NuPAGE 7% Tris-Acetate pre-cast 1.0 mm gels (Invitrogen) for 50 minutes at 150 V and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Developmental Biology 2020Quote: ... Cos-7 cells were transfected with 2.5μg of hexahistidine (His6) tagged TUBA1A plasmid DNA using Lipofectamine 3000 (Invitrogen). For Cos-7 proteasome inhibition assays ...
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bioRxiv - Biochemistry 2021Quote: ... ice cold PBS at 2e6 cells/ml and stained with 7-AAD viability dye (1 ug/mL, Invitrogen). Cell sorting was done on a FACSAria III instrument ...
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bioRxiv - Molecular Biology 2021Quote: ... RNAs were concentrated in an Amicon Ultra 7 kDa filter and buffered exchanged into nuclease free water (Invitrogen), to which the indicated buffer and salt concentrations were added ...
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bioRxiv - Genomics 2021Quote: ... qPCR was performed on Viia 6/7 Real time PCR system using SYBR Green master mix (Life Technologies). The primers used are listed in Supplementary table 1 ...
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bioRxiv - Molecular Biology 2021Quote: ... HepG2 and Huh-7 cells were transfected with miRNA or siRNA using LipofectamineTM RNAiMAX transfection reagent (ThermoFisher Scientific) according to the manufacturer’s instructions and following experiments were performed 48 h after transfection ...
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bioRxiv - Biophysics 2021Quote: ... Cells were finally switched to serum-reduced medium comprising DMEM (HEK293T, COS-7) or FluoroBrite DMEM (Gibco, #A1896702) (HeLa) ...
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bioRxiv - Cell Biology 2021Quote: ... These experiments were carried out with the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems). Expression of circRNAs was calculated relative to the housekeeping genes 18S or ACTB ...
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bioRxiv - Cell Biology 2020Quote: ... Enteroids were passaged every 5 to 7 days by incubating in 0.25% trypsin 0.5 mM phosphate buffered saline-ethylenediaminetetraacetic acid (PBS-EDTA, Gibco) solution for 3 minutes at 37°C ...
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bioRxiv - Immunology 2021Quote: ... Whole bone marrow cultures were then cultured in 10 cm non-tissue culture treated dishes for 7 days in Dulbecco’s modified Eagle’s medium (DMEM) containing 25 mM glucose (Invitrogen) and supplemented with 100 U/ml penicillin and 100 ng/ml streptomycin (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... bone marrow monocytes were immuno-magnetically isolated and culture for 7 days in Dulbecco’s modified Eagle’s medium (DMEM) containing 25 mM glucose (Invitrogen) and supplemented with 100 U/ml penicillin and 100 ng/ml streptomycin (Sigma) ...
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bioRxiv - Immunology 2020Quote: ... polyclonal CD8+ T cells were labelled with 20 μM CellTracker™Blue CMAC (7-amino-4-chloromethylcoumarin; Invitrogen) for 20-30 min at 37° C ...
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bioRxiv - Immunology 2021Quote: ... Real-time qPCR reactions were performed using the QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems).
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bioRxiv - Immunology 2020Quote: ... splenic NP366-374 TMEM were assessed with CellEvent™ Caspase-3/7 Green Flow Cytometry Assay kit (ThermoFisher). Lung single cells were stained with surface markers then incubated with caspase 3/7 green detection reagent for 30 minutes at 37°C as described in the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... siRNAs (3μl of 10μM conc.) and RNAiMax (7 μl) were mixed separately with 75 μl Optimem (Thermofisher, 31985062). Knockdown was initiated by mixing both siRNA and RNAiMAX suspensions together ...
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bioRxiv - Cell Biology 2022Quote: ... Nuclei were counterstained using 4′,6-diamidin-2-phenylindol (DAPI, 1:1000, 7 min; D1306, Thermo Fisher Scientific). Washing steps were performed with PBS ...
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bioRxiv - Cell Biology 2022Quote: WT organoids passage 10 were collected 5-7 days after passaging and digested with TrypLE (Thermo Fisher Scientific) for 20 min at 37 °C ...
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bioRxiv - Neuroscience 2022Quote: Hippocampal neurons were transfected at 7 days in vitro (DIV) using Lipofectamine 2000 (Thermo Fisher, Cat. no. 11668019). Experiments were carried out after 20-24 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... with 7 to 14 cycles of PCR using the Phusion High-Fidelity PCR Master Mix (Thermo Fisher Scientific). The ctDNA yield was quantified with the Qubit™ dsDNA HS Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... Technical replicates for each experimental sample were analyzed in a ViiA 7 Real-Time PCR system (Applied Biosystems) using the standard curve method ...
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bioRxiv - Developmental Biology 2022Quote: ... together with its IgG2a kappa PE/Cyanine 7-Isotype control (1:500, Thermo Fisher Scientific, 25-4321-81), CD31-APC with human specificity for HUVEC experiments (1:500 ...
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bioRxiv - Cell Biology 2022Quote: COS-7 cells (ATCC CRL-1651) were cultured in DMEM (5.56 mM glucose, glutaMAX supplement, Thermo Fisher 10567022) supplemented with 10% fetal bovine serum (ThermoFisher 10438-034) ...
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Dual and Opposing Roles for the Kinesin-2 Motor, KIF17, in Hedgehog-dependent Cerebellar DevelopmentbioRxiv - Developmental Biology 2022Quote: COS-7 cells were transiently transfected with the relevant DNA constructs using Lipofectamine 2000 (Invitrogen, catalog number 11668). The media was collected ...
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bioRxiv - Genomics 2022Quote: ... Reactions were carried out on a QuantStudio 7 Flex 384-well Real-Time PCR System (Thermo Fisher Scientific).
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bioRxiv - Genetics 2022Quote: ... and expression levels were quantified in the QuantStudio™ 7 Flex Real-Time PCR machine (Thermo Fisher Scientific) using 2xSybRGreen Master Mix (Applied Biosystems ...
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bioRxiv - Genetics 2022Quote: ... and expression levels were quantified in the QuantStudio™ 7 Flex Real-Time PCR machine (Thermo Fisher Scientific) using Power SYBR™ Green PCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Fractions of 7 to 12 representing the translation level were taken for RNA isolation with TRIzol reagent (Ambion). To perform RNA-seq experiment ...
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bioRxiv - Systems Biology 2022Quote: ... 4 × 108 cells per biological replicate were used after 7 days of blasticidin selection (10 mg/mL, Gibco), which was 9 days post-infection ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was synthesized from 7 µl of RNA using the High-Capacity RNA-to-cDNA Kit (Thermo Fisher). TaqMan assays (Thermo Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... was added to each well and amplification continued in a QuantStudio 7 Flex qPCR machine (Thermo Fisher Scientific) until signal plateaued out of exponential amplification using the following programme ...
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bioRxiv - Microbiology 2022Quote: ... Real-time PCR assays were conducted using a QuantStudio 7 Pro Real Time PCR system from Thermo Fisher Scientific Inc ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we ran two technical replicate reactions in an QuantStudioTM 7 Flex Real-Time PCR System (Applied Biosystems™). We used 4μL of genomic template ...
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bioRxiv - Developmental Biology 2022Quote: ... Stained cell suspension was passed through a cell strainer and 7-aminoactinomycin D (Molecular probes by Life Technologies) was added for dead cell identification ...
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bioRxiv - Developmental Biology 2022Quote: ... Stained cell suspension was passed through a cell strainer and 7-aminoactinomycin D (Molecular probes by Life Technologies) was added for dead cell identification ...
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bioRxiv - Molecular Biology 2022Quote: ... Quantitative PCR (qPCR) reactions were performed with SYBR green (CWBiotech, CW2621) on the QuantStudio 7 Pro (Applied Biosystems) qPCR instrument ...
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bioRxiv - Microbiology 2023Quote: ... and then primers listed in Supplemental Table 2 on a ViiA 7 real-time PCR system (Applied Biosystems) with the following cycling parameters ...
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bioRxiv - Microbiology 2023Quote: ... the cells were loaded with 2 µM CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific), according to the manufacturer’s instructions for kinetic assays and fluorescence in the live cells ...
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bioRxiv - Immunology 2023Quote: ... Cells were differentiated for 7 days in RPMI 1640 medium with L-glutamine (Gibco Thermo Fisher Scientific 21875034) supplemented with 5% heat-inactivated fetal bovine serum (Biosera FB-1001 ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were washed twice and resuspended in FACS buffer containing 7-AAD viability staining solution (Life Technologies). Cell sorting was performed using a BD FACSAria III (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... Cells were differentiated for 7 days in RPMI 1640 medium with L-glutamine (Gibco Thermo Fisher Scientific 21875034) supplemented with 5% heat-inactivated fetal bovine serum (Biosera FB-1001 ...
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bioRxiv - Neuroscience 2023Quote: ... equal amounts of protein were loaded in 7% Bis-Tris gels and run with MOPS buffer (Thermo Scientific) and transferred to membranes (Amersham GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... washed with 1 x PBS (pH 7) and subsequently labelled with 25 μg/ml AF488 C5 Maleimide (Invitrogen) for 10 min in the dark followed by at least two additional washing steps ...
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bioRxiv - Developmental Biology 2022Quote: ... Quantitative PCR for pri-let-7 and eft-2 were performed with Absolute Blue SYBR Green (Thermo Scientific) on the CFX63 Real Time System Thermocyclers (Biorad ...
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bioRxiv - Molecular Biology 2023Quote: ... The qRT–PCR was performed using a QuantStudio™ 7 Flex Real-Time PCR System (Thermo Fisher Scientific). The sequences of primers and probes for Adar1 p150 and GAPDH have been previously reported (16) ...
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: ... Real-time quantitative RT-PCR (Q-PCR) was performed in a QuantStudio 7/12k Flex System (Applied Biosystems) with the following primers Crbn.F 5’-TGAAATGGAAGTTGAAGACCAAGATAG-3 ...
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bioRxiv - Developmental Biology 2023Quote: ... Aggregation medium (BSA supplemented N2B@7 media) consisted in 500ml 1:1 mix of Neurobasal (Invitrogen 21103-049) and DMEM/F12 (Invitrogen 21331) ...
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bioRxiv - Neuroscience 2023Quote: ... 7×106 cells were plated in 12ml of transfection media (Opti-MEM I Reduced Serum Medium (Gibco #31985070), 1x GlutaMAX Supplement ...
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bioRxiv - Biophysics 2023Quote: ... potassium phosphate buffer (KP) 0.5 M at pH = 7 (adjusted with pH meter Orion Star A111, Thermo Fisher). BSA and urease stock solutions concentration are measured after their preparation using a UV-vis spectrophotometer (Cary 60 Spectrophotometer ...
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bioRxiv - Cell Biology 2023Quote: ... MCF-7 cells (HTB-22) and A431 cells (CRL-1555) were all cultured in DMEM (Gibco, 31966-021) supplemented with 10% FCS (Gibco ...
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bioRxiv - Immunology 2023Quote: ... iGB cells were collected after 6-7 days on iGC culture and were stained with Fura-2 (Invitrogen) at 1μM / ml ...