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Citations for Thermo Fisher :
1451 - 1500 of 2521 citations for 7 Octenyldimethylmethoxysilane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Following buffer exchange to 100 mM NaHCO3 with 0.5 ml 7 kDa Spin Desalting Columns (Thermo Fisher, 89882), the anti-TFAM (TA332462 ...
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bioRxiv - Cell Biology 2020Quote: ... 7- Amino-4-Chlormethylcumarin (CMAC) was part of the Yeast Vacuole Marker Sampler Kit (Y7531) from Thermo Fisher and staining was performed according to manufacturer recommendations ...
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bioRxiv - Physiology 2019Quote: ... Malpighian tubules from 7-day-old adult female flies were dissected under Schneider’s insect medium (Thermo Fisher Scientific) and isolated into 10 μl drops of a 1:1 mixture of Schneider’s medium ...
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bioRxiv - Synthetic Biology 2019Quote: ... 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) was purchased from Invitrogen. Calcein dye ...
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bioRxiv - Cell Biology 2019Quote: ... q-PCR was performed using a ViiA 7™ Real Time PCR System (Applied Biosystems, Life Technologies, UK) programed to perform the following steps ...
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bioRxiv - Cell Biology 2019Quote: ... q-PCR was performed using a ViiA 7™ Real Time PCR System (Applied Biosystems, Life Technologies, UK) programed to perform the following steps ...
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bioRxiv - Bioengineering 2021Quote: ... Real-time polymerase chain reaction (real-time PCR) was performed on duplicates using QuantStudio 7 Flex (Applied Biosystems, Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... roots of 7-day-old seedlings were incubated with 50 µM Amplex™ UltraRed Reagent (AUR, Molecular Probes) for 20 min in dark conditions and rinsed with liquid MS ...
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bioRxiv - Microbiology 2021Quote: ... mRNA expression of the indicated genes was analyzed by QuantStudio 7 Flex Real Time PCR System (Applied Biosystems) using SYBR green master mix ...
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bioRxiv - Microbiology 2020Quote: ... Vero E6 and Huh-7 cells (Apath LLC, Brooklyn) were maintained in Dulbecco’s modified eagle medium (DMEM, Gibco) supplemented with 10 % heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2021Quote: EV-rich fractions (7-10) from SEC were initially determined by protein (Pierce™ BCA Protein Assay, ThermoFisher) and particle quantification using nanoparticle tracking analysis (NTA ...
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bioRxiv - Bioengineering 2021Quote: ... Apoptosis was detected by incubating devices with 5μM of CellEvent™Caspase 3/7 Green detection reagent (Invitrogen) for 30 minutes at 37°C.
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bioRxiv - Biochemistry 2020Quote: ... Hs00961560_m1 Hs INSR) and the QuantStudio™ 7 Flex Real-Time PCR system (Thermo Fisher Scientific, MA, USA) and results analysed by the comparative Ct method ...
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bioRxiv - Cancer Biology 2020Quote: ... were used to analyse mRNA expression levels using a QuantStudio 7 Flex RT PCR machine (Thermo Fisher Scientific). TaqMan probes used were FAP (Hs00990791_m1) ...
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bioRxiv - Physiology 2021Quote: ... Reactions were performed in a total volume of 10 μl on a ViiA 7 system (Thermo Fisher Scientific). Gapdh was used as a housekeeping gene and data were evaluated by conversion to ΔCt values.
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bioRxiv - Cell Biology 2021Quote: ... Both COS-7 and HEK 293T were transfected by means of Lipofectamine and PLUS reagent (Thermo Fisher Scientific). The calcium phosphate transfection method was instead used to transfect WT or ΔOLF Lphn2-HA-pCCL in HEK 293T cells and to produce the corresponding viral particles subsequently employed to transduce ECs ...
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bioRxiv - Microbiology 2021Quote: ... a 20μL reaction was loaded into a ViiA 7 Real Time PCR system (Thermo Fisher Scientific, Waltham, MA). The following conditions were used ...
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bioRxiv - Molecular Biology 2021Quote: ... quantitative RT-PCR analysis was performed using a sequence detection system (ABI ViiA 7 system; Thermal Fisher Scientific). The following primers sets were used for analysis ...
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bioRxiv - Neuroscience 2020Quote: ... 2x SYBR master mix (Thermoscientific) were used and the reaction was run in Thermocycler (Applied Biosystems ViiA 7) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... MEL cell line-derived clones 4D7 and 2M12 (7) were maintained in 20 µg/mL of blasticidin (Invitrogen) and 1 mg/mL of G418 (Gibco) ...
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bioRxiv - Neuroscience 2020Quote: NO production was detected by DAF-FM diacetate (4-amino-5-methylamino-2’,7’-difluorofluorescein diacetate) (D23844, ThermoFisher). Fly larvae were dissected at 24 or 48 h AI in PBS to expose the sensory neurons ...
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bioRxiv - Biophysics 2020Quote: Microinjection was performed in COS-7 cell line cultured in 10% (v/v) fetal bovine serum (Life technologies) DMEM at 1·105 cell/well density ...
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bioRxiv - Cancer Biology 2020Quote: ... BT-12 and BT-13[7] were cultured in Dulbecco’s modified Eagle’s medium with Nutrient Mixture F-12 (DMEM/F12, Gibco) supplemented with 2 mM L-glutamine ...
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bioRxiv - Cancer Biology 2021Quote: ... After 3 and 7 days of treatment cells were stained with 2% crystal violet (CV) (40583100, Acros Organics, Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... COS-7 cells (ATCC) were grown at 37°C with 5% CO2 in Dulbecco’s modified Eagle’s medium (Gibco) containing 10% fetal bovine serum and 1% penicillin/streptomycin (Invitrogen) ...
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bioRxiv - Bioengineering 2022Quote: ... were used for experiments in passage 4 to 7 and were cultivated in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... 5 μl of 7-amino-actinomycin D (7AAD, 50 μg/ml working solution in PBS, Thermo Fisher Scientific) was added to the stained cells 5–10 min before measurement.
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bioRxiv - Physiology 2022Quote: ... the fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG; 6.83 μg/kg BW, Invitrogen) dissolved in 50% dextrose (1 g/kg BW ...
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bioRxiv - Microbiology 2022Quote: ... infected Jurkat cells were initially preloaded with 5 µM of CellTracker CMAC (7-amino-4-chloromethylcoumarin) (Life Technologies) and cocultured for 6 or 24 h with MDMs plated onto coverslips ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were incubated for 30 min in standard media with CellEvent Caspase 3/7 (1:400, C10423, Invitrogen) prior to imaging to observe apoptosis.
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bioRxiv - Bioengineering 2022Quote: ... COS-7 cells were plated onto Nunc Lab-Tek II Chambered Coverglass (Thermo Fisher Scientific, 12-565-335) to achieve ~70% confluence before transfection.
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bioRxiv - Neuroscience 2022Quote: ... Tax ID 10090) protein database (2017-6-7) using Proteome Discoverer (PD) 2.2 (Version 2.2.0.388; Thermo Fisher Scientific). Label-free quantification was also performed with PD 2.2 using precursor ions quantifier nodes ...
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bioRxiv - Cell Biology 2022Quote: ... and cultured for 5-7 days at P0 before been passaged with Tryspin - EDTA 0.05% (Thermo Scientific, 25300054). Cells were passaged every 4-5 days and until p8 ...
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bioRxiv - Cancer Biology 2020Quote: ... qRT-PCR assays were performed in technical quadruplicates in 384-well plates on QuantStudio 7 instrument (Life Technologies), with RT2 SYBR Green (Qiagen ...
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bioRxiv - Neuroscience 2021Quote: ... Pipettes had resistances of 5–7 MΩ when filled with this solution supplemented with Fura-2 (Molecular Probes). Recordings were made using a Multiclamp 700B amplifier (Molecular Devices ...
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bioRxiv - Microbiology 2020Quote: ... prehybridization was carried out for 2 h at 42°C in 7 mL of prehybridization buffer ULTRAHyb (Ambion). Hybridization was performed overnight at 42°C in the same buffer in the presence of a [γ-32P]-labeled DNA oligonucleotide probe ...
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bioRxiv - Microbiology 2021Quote: ... Unbound GTP was removed using 2 mL Zeba column with 7 kDa molecular weight cutoff (Thermo Scientific, 89889) and exchanged into 30 mM Tris pH = 7.5 buffer containing 1 mM DTT ...
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bioRxiv - Cancer Biology 2022Quote: ... 7% glacial acetic acid in water for 15 min and stained with GelCode Blue Stain (Thermo Fisher Scientific). For in-gel digestion ...
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bioRxiv - Molecular Biology 2022Quote: ... Quantitative Polymerase Chain Reaction (qPCR) was performed in a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems) using SYBR Green Real-Time PCR Master mix (Invitrogen ...
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bioRxiv - Biophysics 2022Quote: ... Purification was performed using spin columns (7 kDa exclusion limit, 0.5 mL Zeba spin desalting columns, ThermoFisher Scientific). After purification ...
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bioRxiv - Cell Biology 2022Quote: Plasmids were transiently transfected into HEK293T or MCF-7 cells at 70% confluence using Lipofectamine 3000 (Invitrogen, #L3000015) in a 4:1 v/w ratio following manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: MA104-NSP5-EGFP cells (MA-NSP5-EGFP) (7) were cultured in DMEM supplemented with 10% FBS (Life Technologies), 50 μg/ml gentamycin (Biochrom AG ...
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bioRxiv - Cancer Biology 2019Quote: ... The RT-qPCR assays were completed using the ABI ViiA™ 7 sequence detection system (Applied Biosystems®) with the following parameters ...
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bioRxiv - Microbiology 2019Quote: ... Haematopoietic stem cells differentiated into BMDMs by incubating for 7 days in BMDM medium (1:1 SFM:DMEM, Gibco) supplemented with 10 % FCS (Biochrom) ...
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bioRxiv - Cancer Biology 2020Quote: ... Each murine sample was analyzed in triplicate on ViiA™ 7 Real-Time PCR System (Applied Biosystems®). Data generated was normalized to β-actin.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and Huh 7 engineering by lentivirus transduction to overexpress the human ACE2 (Huh-7hACE2) were cultured in Dulbecco’s modified 7 Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Gibco). Working stocks of SARS-CoV-2 ICGEB-FVG_5 isolated in Trieste ...
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bioRxiv - Neuroscience 2021Quote: ... Forty cycles of amplification were run on a Quant Studio 7 Flex Real-Time PCR System (Applied Biosystems) and the resulting data was analyzed using the comparative ΔΔCT method (Livak and Schmittgen ...
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bioRxiv - Bioengineering 2020Quote: ... WT-PGP1 were labeled with 5 μM CellTracker™ Blue 7-amino-4-chloromethylcoumarin CMAC (Molecular Probes, #C2110), iNeuron-PGP1 was labeled with 2.5 μM CellTracker™ Green CMFDA (Molecular Probes ...
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bioRxiv - Microbiology 2019Quote: ... at a final DNA concentration of 0.4 ng/μl using the ViiA 7 Real Time PCR System (ThermoFisher). Amplification conditions were as follows ...
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bioRxiv - Genetics 2021Quote: ... and expression levels were quantified in the QuantStudio(tm) 7 Flex Real-Time PCR machine (Thermo Fisher Scientific) using 2xSybRGreen Master Mix (Applied Biosystems ...