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Citations for Thermo Fisher :
1451 - 1500 of 5645 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: NIH3T3 derived Bmal1-luc31 and HEK293/T17 cells were cultured with Dulbecco’s Modified Eagle Medium (DMEM) (Gibco: Cat #11965118) supplemented with 10 % fetal bovine serum (Corning ...
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bioRxiv - Immunology 2024Quote: ... HEK293T TRIM21 knock-out and HEK293 HOIP KO cells were maintained at 37°C in complete DMEM (ThermoFisher 10569010) supplemented with 10% FBS and pen/strep.
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bioRxiv - Neuroscience 2024Quote: ... DRG neurons or HEK293 cells were incubated with 5 μM Fura-2 AM and 0.2% Pluronic (Thermo Fisher Scientific) (prepared from a 200 mg/ml stock solution in DMSO ...
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bioRxiv - Biochemistry 2024Quote: HEK293 cells (ATCC CRL-1573) were seeded in 6-well plates with Dulbecco’s Modified Eagle Medium (DMEM; Gibco, 11965092) supplemented with 10 % FBS (Sigma–Aldrich ...
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bioRxiv - Biophysics 2024Quote: ... Stable cell lines were made by transfecting HEK293 cells with the respective plasmids using Lipofectamine 2000 (Invitrogen, Waltham, MA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: HEK293 cells were maintained in high glucose Dulbecco’s modified Eagle’s medium 5 g/L glucose (DMEM; Gibco, UK, 11965084). Cell culture media were supplemented with 10% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... was maintained in DMEM cell culture medium with the addition of 10% m/m Fetal Bovine Serum (FBS, Gibco/ThermoFisher, CA, USA) supplemented with 50 U/ml penicillin and 5 µl/mL streptomycin ...
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bioRxiv - Plant Biology 2020Quote: ... and an Acclaim® PepMap analytical column (ID: 75 m, 500 mm, 2 m, 100 Å, Dionex LC Packings/Thermo Fisher Scientific).
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bioRxiv - Zoology 2022Quote: ... or 5,6,11-trideoxyTTX (10−5 M) together with a dextran conjugated with Alexa Fluor 555 (M.W. 10,000, 10−6 M; D34679, Thermo Fisher Scientific, MA, USA) for 5 min ...
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bioRxiv - Molecular Biology 2019Quote: ... were separated on the EASY-Spray Column Acclaim PepMapTM C18 100A□ (2□m particle size, 50□m id × 25cm; Thermo Fisher Scientific) by a gradient from 5% to 40% of buffer B (0.1% formic acid in acetonitrile ...
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bioRxiv - Cell Biology 2020Quote: ... Capillary columns had an inner diameter of 100 ☐m and were packed with 2.6 ☐m Accucore beads (Thermo Fisher Scientific). Peptides were analyzed on acidic acetonitrile gradients for 5 h with MS1 (Orbitrap ...
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bioRxiv - Biophysics 2023Quote: ... All reactions were performed at at room temperature in ultrapure water adjusted to pH~8.5 using 1 M sodium bicarbonate (Fisher Scientific, M-14636) for 3 h ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 g faecal material was resuspended in a 10 ml SM buffer (50 ml 1 M UltraPure™ 1 M Tris-HCI Buffer, pH 7.5 (Invitrogen™ #15567027); 20 ml 5 M NaCl (Sigma-Aldrich Cat#221465) ...
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bioRxiv - Immunology 2024Quote: ... The purified mAbs were dialyzed against phosphate-buffered saline (PBS, 0.1 M sodium phosphate, 0.15 M sodium chloride, pH 7.2) (Thermo Fisher Scientific, Waltham, MA, USA) containing 0.02% sodium azide in dialysis tubing with 6-8 kDa cutoff limit (Spectrum Chemical ...
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bioRxiv - Bioengineering 2019Quote: ... and stained with IL2-XE114-TNFmut (final concentration 10μg/mL) and detected with rat anti-IL2 (eBioscience 14-7029-85) followed by staining with anti-rat AlexaFluor488 (Invitrogen A21208). IL2-KSF-TNFmut (specific for an irrelevant antigen ...
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bioRxiv - Cell Biology 2019Quote: ... 3 rat anti-Rab1 and 3 rat anti-Rab11 (1:2000 concentrated supernatant) as primary antibodies and HRP-conjugated anti-rat IgG antibodies (1:20000, Life Technologies) as secondary antibodies ...
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Impairment of a distinct cancer-associated fibroblast population limits tumour growth and metastasisbioRxiv - Cancer Biology 2020Quote: ... single cells were re-suspended at a density of 1-2 × 107 and subjected to magnetic sorting to exclude immune cells using rat-anti-mouse CD45 (R&D, MAB114) and CD24 (eBioscience, clone M1/69) magnetic sheep-anti-rat Ig Dynabeads (Invitrogen, #11035). FACS for FITC+/RFP-/DAPI-was used to obtain GFP+ve CAFs and to exclude RFP+ve tumour cells and dead cells ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained with unconjugated lineage rat antibodies (CD3, CD4, CD5, CD8, B220, Gr1, Mac1, and Ter119) followed by goat-α-rat PE-Cy5 (Invitrogen). Stem and progenitor cells were isolated using fluorescently labeled or biotinylated antibodies for the following antigens ...
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bioRxiv - Neuroscience 2022Quote: ... The expression of Kir2.1EGFP was assessed with anti-GFP antibody (rat monoclonal Ab from Nacalai, Japan, GF090R; the secondary antibody, 488-anti Rat IgG from Invitrogen, A11006) (Mizuno et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... bakeri worm lysates or EVs were carried out with rat polyclonal anti-exWAGO antibody (raised against full length protein) or rat IgG (control) using protein L magnetic beads (Fisher Scientific). Equivalent amounts of the input ...
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bioRxiv - Neuroscience 2024Quote: ... To visualize Gi-DREADD-mCherry expression in rCA2 of injected mice sections were stained with 1⁰ rat anti-mCherry (Life Technology #M11217) 1:500 + 2⁰ anti-rat 568 (Invitrogen #A11077) at 1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... In order to visualize AAV-retro positive cells sections were stained for mCherry (1⁰ rat anti-mCherry (Life Technology #M11217) 1:500 + 2⁰ anti-rat 568 (Invitrogen #A11077) at 1:250 ...
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bioRxiv - Cancer Biology 2021Quote: ... 200 units of M-MLV reverse transcriptase (ThermoFisher 28025013) and 250 ng of Pd(N)6 random hexamers (Euromedex PM-301L) ...
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bioRxiv - Biophysics 2020Quote: ... 0.1 M NaCl and 1% DDM (Anatrace, Affymetrix, USA) and stirred overnight for solubilization ...
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bioRxiv - Cell Biology 2020Quote: ... 100 mg of Dynabeads® M-270 Epoxy (Invitrogen) were transferred to a 15 ml centrifuge tube ...
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bioRxiv - Molecular Biology 2021Quote: ... immobilized on 40 µl Streptavidin M-280 Dynabeads (Invitrogen). After 10 minutes incubation of chromatin with antibody-resin conjugate ...
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bioRxiv - Developmental Biology 2021Quote: Dynabeads® M-270 Epoxy (Invitrogen Life TechnologiesTM, 14301) were prepared in order to directly crosslink antibodies to the beads (anti-GAF ...
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bioRxiv - Molecular Biology 2021Quote: ... Porcine oocytes were cultured in M-199 MEDIUM (Gibco) supplemented with 1 mM dbcAMP ...
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bioRxiv - Neuroscience 2021Quote: ... 2 M thiourea and 4% CHAPS (Thermo Fisher Scientific). Each combined replicate of samples was loaded onto a QIAshredder spin column (QIAGEN ...
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bioRxiv - Immunology 2022Quote: ... cells were lysed in M-PER buffer (Thermo Scientific) containing protease/phosphatase inhibitor cocktail (Roche) ...
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bioRxiv - Genomics 2019Quote: ... 250 µl Dynabead M-270 Streptavidin (65306, ThermoFisher Inc) was washed on a magnetic stand three times in 2xSSC ...
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bioRxiv - Biophysics 2019Quote: ... M-280 Tosyl activated Dynabeads (14203) were from Invitrogen. SDS-PAGE analysis was performed with either Bolt 4-12% Bis-Tris Plus gels (Life Technologies ...
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bioRxiv - Bioengineering 2021Quote: ... UltraPure™ 0.5-M EDTA (15575020, Thermo Fisher Scientific), Y-27632 (08945-71 ...
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bioRxiv - Bioengineering 2021Quote: ... 5 × 10−5 M 2-mercaptoethanol (Gibco, 31350-010), 1X Minimal Essential Medium (MEM ...
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bioRxiv - Bioengineering 2021Quote: ... 5 × 10-5 M 2-mercaptoethanol (Gibco, 31350-010), 1X Minimal Essential Medium (MEM ...
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bioRxiv - Biophysics 2020Quote: ... and 55 μ M β -mercaptoethanol (Invitrogen, Carlsbad, CA). pET28a vector was a gift from Dr ...
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bioRxiv - Cancer Biology 2020Quote: ... and M-MLV reverse transcriptase (cat # 28025-013, Invitrogen) were used for cDNA synthesis as per manufacturer’s protocol.
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bioRxiv - Cancer Biology 2020Quote: M-280 Streptavidin Dynabeads® (Invitrogen, catalog no. 11205D) were washed according to the manufactures recommendations ...
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bioRxiv - Plant Biology 2020Quote: ... Dynabeads M-280 Sheep Anti-Rabbit IgG (Invitrogen 11203D) or Anti-Mouse IgG (Invitrogen 11201D ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and 252 ul 1 M HEPES buffer (ThermoFisher Scientific), and stored frozen up to 1 year ...
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bioRxiv - Plant Biology 2021Quote: ... and Dynabeads M-270 Streptavidin (Thermo Fisher Scientific, USA). Finally ...
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bioRxiv - Microbiology 2020Quote: Cells were lysed using M-PER reagent (ThermoFisher 78501) with complete protease inhibitor cocktail (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... One minute of methanol (Fisher Scientific, M/3900/17) immersion was done ...
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bioRxiv - Cell Biology 2022Quote: ... and M-MLV Reverse Transcriptase kit (Thermo Fisher Scientific). RT-PCR was performed using the G-Storm thermal cycler on 25 µl reaction samples containing forward and reverse primers (200 nM ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-MUC1 (1:100, Thermo Scientific, M A5-11202), D onkey a nti-Goat IgG (H+L ...
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bioRxiv - Microbiology 2022Quote: ... in 0.2 M HEPES (pH 7.2–7.4, Gibco 15630080) for 20 min at room temperature ...
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bioRxiv - Genetics 2022Quote: ... and 1 μL M-MLV reverse transcriptase (ThermoFisher Scientific) were then added and cDNA conversion was performed in a thermocycler (37°C for 50 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... containing 300 μL M-450 Epoxy Dynabeads (14011, Invitrogen). Both kidneys were removed and minced into small pieces with a scalpel and incubated in 3 ml prewarmed digestion buffer (1.5 U/ml Liberase TM ...
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bioRxiv - Systems Biology 2019Quote: ▪ Parafilm M (Fisher Scientific, cat. no. 13-374-10)
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bioRxiv - Plant Biology 2019Quote: ... 100ul of Dynabeads M-280 Streptavidin beads (Thermo Scientific) prebound with or without the N-terminal biotinylated PNP-A peptide or its scrambled peptide ...