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Citations for Thermo Fisher :
1551 - 1600 of 5645 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Streptavidin-coated M-280 Dynabeads (Thermo Fisher; cat. #112.50) (20 µL slurry volume ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was generated using M-MLV Reverse Transcriptase (Invitrogen). Qiagen Taq polymerase (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.001 M sodium pyruvate (Thermo Fisher Scientific, Cat# BP356), and 0.1 mg/ml DNase (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 μl Dynabeads™ M-280 Streptavidin (ThermoFisher, 11205D) for each sample was washed three time with 2xB&W Buffer (10 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Molecular Biology 2024Quote: ... was conjugated to Epoxy Dynabeads™ M-270 (InVitrogen) at a ratio of 20µg per 1mg beads in 0.1M Na-Phosphate buffer ...
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bioRxiv - Microbiology 2024Quote: ... and 500 µL of ampicillin (Fisher Scientific, Waltham, M) at 100 mg/mL ...
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bioRxiv - Neuroscience 2024Quote: μM MitoSox-Red (Thermo Fisher Scientific, Washington, DC; #M36008) in 1x PBS for 10 minutes at room temperature protected from light ...
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bioRxiv - Immunology 2024Quote: ... Complementary DNA was prepared using M-MLV RT (Invitrogen), and quantitative PCR (qPCR ...
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bioRxiv - Immunology 2024Quote: ... 200U of M-MLV reverse transcriptase (18057018, ThermoFisher Scientific) and 40U of RNAse OUT inhibitor (10777019 ...
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bioRxiv - Cancer Biology 2024Quote: ... NaCl (0.2 M final concentration; Fisher Scientific, BP358-10) was added ...
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bioRxiv - Cancer Biology 2023Quote: ... Dynabeads™ M-280 sheep anti-Mouse IgG (Invitrogen), Anti-Sulf-2 monoclonal antibodies (QED Bioscience) ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was synthesized using M-MLV Reverse Transcriptase (Invitrogen) and random hexamers (NEB) ...
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bioRxiv - Cell Biology 2021Quote: ... 100 μL reactions containing 10 μL FLAG-xHJURP truncations or mutants in CSF-arrested or interphase extract were added to 10 μL Protein A Dynabeads (Invitrogen) coupled to 2.5 μg mouse anti-FLAG antibody ...
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bioRxiv - Microbiology 2020Quote: ... Washed PBMC was then replenished with 5 ml of R10 supplementing with 2,000 U/ml of rh GM-CSF (ThermoFisher Scientific) and 4,000 U/ml of rh IL-4 (R&D Systems ...
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bioRxiv - Immunology 2021Quote: ... or anti-inflammatory (Mφ2) macrophages with 5 ng/ml of granulocyte-macrophage colony- stimulating factor (GM-CSF; Life Technologies-Invitrogen) or 50 ng/ml macrophage colony- stimulating factor (M-CSF ...
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bioRxiv - Immunology 2022Quote: ... 2×107 murine bone marrow progenitors were seeded in 100 ml RPMI-FBS supplemented with 10% J558-conditioned medium containing Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) in 500 cm2 square petri dishes (Nunc). Cells were incubated at 37°C in 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... were coated with capture antibodies diluted in 1X PBS: anti-mouse GM-CSF Capture (Invitrogen, #88-7334-88, 1:250), anti-mouse IL-10 capture (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Endogenous SART1 was depleted from CSF extracts by 2 rounds of incubation with 60% (vol/vol) Dynabeads Protein A (Invitrogen) coupled with Xenopus SART1 antibodies.
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bioRxiv - Cell Biology 2023Quote: ... Monocytes were differentiated into GM-CSF-polarised (M1) MDMs over seven days in RPMI 1640 media supplemented with 1 mM L-glutamine (Gibco), 2 mM sodium pyruvate ...
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bioRxiv - Molecular Biology 2023Quote: ... γ-TuRC was biotinylated prior to elution by incubation for 1 hour on ice in CSF-xB 2% (w/w) sucrose supplemented with 40 μM NHS-PEG4-biotin (ThermoFisher). The elution was concentrated using a 100 kDa MWCO spin concentrator (Amicon) ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of CSF were loaded and separated by electrophoresis in a NuPAGE 4–12% Bis-Tris gel (Invitrogen #NP0322), transferred to a nitrocellulose membrane (250 mA ...
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bioRxiv - Systems Biology 2023Quote: 1μL of plasma and 150μL of CSF were depleted with depletion columns (High Select™ Depletion Spin Columns, A36370, ThermoFisher). The filtrate was collected and evaporated to dryness on SpeedVac (50 °C) ...
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bioRxiv - Immunology 2023Quote: To assess thermodynamic stability of human and bat (M. myotis and M. capaccinii) purified IgG molecules we used Protein Thermal Shift Dye Kit (Applied Biosystems, Thermo Fisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: Extracted ion chromatograms of the [M-H]- (dA, dT, dC, 5hmdC, 5fdC, 5cadC)/[M+H]+ (5ghmdC) forms were integrated using Tracefinder software (ThermoFisher Scientific, MA, USA). Relative abundance in each sample was calculated by normalizing the peak area of each peak by the peak area of the dG signal in each specific sample ...
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bioRxiv - Microbiology 2021Quote: ... and mACP-HA2 was visualized with the rat anti-HA 3F10 primary antibody and FITC-conjugated donkey anti-rat 2° antibody (Invitrogen Life Technologies A18746). Images were taken on an EVOS M5000 imaging system or Zeiss 880 laser-scanning confocal microscope fitted with an Airyscan detector ...
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bioRxiv - Microbiology 2023Quote: ... and the endogenously C-terminal HA-tagged cytochromes were visualized with a Roche Rat anti-HA monoclonal 3F10 primary antibody and FITC-conjugated Donkey anti-Rat 2° antibody (Invitrogen Life Technologies A18746). Images were taken on DIC/brightfield ...
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bioRxiv - Microbiology 2023Quote: ... Lysates and immunecomplexes were separated by SDS-PAGE and blotted to detect either biotynlated proteins (IRDye® 680LT streptavidin (Li-Cor)) or CDPK3HA (rat-anti-HA)/anti-rat DyLight™ 800 (Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... Alexa488 anti-rat and Cy3 anti-rat (1:250, Jackson) secondary antibodies or with Alexa647-conjugated Streptavidin (1:200, Thermo Fisher Scientific) and by counterstaining with DAPI (0.2 µg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... sera samples and conditioned tissue culture medium from rat hepatic stellate cells (HSCs) or primary rat hepatocytes (PCs) were stained with phycoerythrin (PE)-labeled Cell Mask (Thermo Fisher Scientific) or stained with Syto RNASelect (Thermo Fisher Scientific) ...
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bioRxiv - Pathology 2023Quote: ... appropriate secondary antibodies were treated for 1-2 hours in the dark (AF555 anti-Rabbit, AF555 anti-Rat, AF647 anti-Rat (Invitrogen, Waltham, MA)) and washed in PBS containing 0.01% Triton X-100 ...
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bioRxiv - Immunology 2023Quote: ... the appropriate secondary antibodies were added diluted in 1% BSA in PBS for 1 hour at a 1:1000 dilution (Goat anti Rat 488 Thermo Fisher Scientific a11006, Goat anti Rat 647 Thermo Fisher Scientific a21247 ...
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bioRxiv - Cell Biology 2020Quote: ... A Lsm12-KO cell line of HEK293 cells was generated with Synthego’s chemically modified sgRNA 5’-CCAGAAUGUCCCUCUUCCAG-3’ and GeneArt Platinium Cas9 nuclease (ThermoFisher Scientific) that were transfected together into cells using Lipofectamine CRISPRMAX Cas9 transfection reagent (ThermoFisher Scientific).
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bioRxiv - Cancer Biology 2021Quote: ... After 24 hours the media was removed from the HEK293 CLK (NL-CLK1, CLK2-NL or CLK4-NL) transfected cells and replaced with 85 μL OPTI-MEM media (Gibco). NanoBRET Tracer 5 (Promega ...
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bioRxiv - Immunology 2022Quote: ... HEK293 cells stably transfected with human CXCR4 (HEK293- CXCR4) were used at passage 5 and were cultivated in DMEM medium (Gibco), supplemented with 10% FCS and 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Molecular Biology 2020Quote: ... The wild-type complemented HEK293 RAD51 S14A mutant was generated by co-transfecting pcDNA5/FRT encoding wild-type RAD51 and pcDNA-DEST26 (Invitrogen), followed by single cell sorting (FACS ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 cells were purchased from ATCC and cultivated in DMEM medium supplemented with 10% fetal calf serum (Thermo Fisher Scientific). Cells were maintained at 37°C ...
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bioRxiv - Neuroscience 2020Quote: The HEK293 cells (CRL-1573, ATCC, USA) were kept in the Dulbecco’s Modified Eagle Medium (High Glucose)-DMEM (Invitrogen, USA) supplemented with 10% fetal bovine serum-FBS (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 Phoenix cells (National Gene Vector Biorepository, Indianapolis, IN) were transfected with each construct using Lipofectamine 3000 (Thermo Fisher Scientific) per manufacturer’s recommendation ...
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bioRxiv - Biochemistry 2019Quote: ... Transient transfection of 2N3T-Tip60 or the corresponding control empty vector was carried out for 48 hours into HEK293 cells using Lipofectamine 2000 transfection reagent (Invitrogen); UV irradiation was carried out at a dose of 15mJ/cm2 using Stratagene Stratalinker 1800 ...
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bioRxiv - Neuroscience 2019Quote: HEK293 cells (ATCC Cat# CRL-1573) were maintained at 37° C and 5% CO2 in 1X DMEM medium (Thermo Scientific; Waltham ...
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bioRxiv - Molecular Biology 2019Quote: Expression constructs for repeat RNAs or GFP-tagged codon-optimized DPRs were transfected in HEK293 cells or U-2 OS cells with Lipofectamine 2000 (Invitrogen) or FuGENE HD (Promega) ...
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bioRxiv - Molecular Biology 2019Quote: ... pEGFP-C2-IQGAP1 and pcDNA3/RH-p122RhoGAP/DLC-1 plasmids were transfected into HEK293 cells using Lipofectamine 3000 (Invitrogen, MA).
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bioRxiv - Microbiology 2021Quote: ... Plasmids encoding the SARS-COV-2 spike glycoprotein or its mutated variants were cloned into a G418 resistance gene containing backbone and transfected into HEK293 cells using Lipofectamine 2000 (Thermofisher) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... P2 or P3 baculovirus was produced in Sf9 cells and used to transfect HEK293S GnTI- suspension cells cultured in Freestyle 293 (Gibco) with 2% FBS at 37°C with 8% CO2 ...
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bioRxiv - Neuroscience 2020Quote: ... The inter-molecular (oligomer) and intra-molecular (monomer-conformation) aSyn FRET biosensors were generated by transiently transfecting HEK293 cells using Lipofectamine 3000 (Invitrogen) with GFP-aSyn and aSyn-RFP (1:8 DNA plasmid concentration ratio ...
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bioRxiv - Systems Biology 2021Quote: ... HEK293 and Ptk2 cell lines were cultured in DMEM (high glucose, pyruvate) supplemented with 10 % FBS and antibiotic-antimycotic (Invitrogen) and passaged routinely ...
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bioRxiv - Systems Biology 2020Quote: HEK293 cell lines 293-F and Freestyle 293-F were cultivated in Freestyle 293 expression medium (Gibco, Thermo Fisher Scientific) at 37°C ...
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bioRxiv - Systems Biology 2020Quote: HEK293 cell lines 293-F and Freestyle 293-F were cultivated in Freestyle 293 expression medium (Gibco, Thermo Fisher Scientific) at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 cells that express mNRK in a dox-dependent manner were developed using Flp-In T-REx 293 cells (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... pX330 plasmid harboring a p53-specific gRNA (Supplementary Table 1) was transfected into HEK293 and HCT116 cells using Lipofectamine 2000 (Invitrogen). Two days later ...