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Citations for Thermo Fisher :
1301 - 1350 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: HEK293 cells (ATCC CRL-1573) were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Paneco) supplemented with glutamine (Gibco), 10% fetal bovine serum (HyClone) ...
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bioRxiv - Immunology 2023Quote: HEK293 cells were transfected with pcTBK1-His or pcGSK3B-Flag plasmid using Lipofectamine 2000 (Invitrogen, Waltham, MA, US) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... which then were co-transfected into HEK293 6E cells for expression together following standard protocols for 293Fectin (Invitrogen). The cell cultures were harvested at 5 d post-transfection ...
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bioRxiv - Molecular Biology 2022Quote: HEK293-6E cells (used for protein expression) were cultured in expression medium (FreeStyle F17 (Gibco, Cat#A13835-01)) supplemented with 1% FBS (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293 cells were transiently transfected with 1 µg of each cDNA using Lipofectamine 2000 (Invitrogen, Carlsbad CA, USA). After 12 h of transfection ...
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bioRxiv - Genomics 2023Quote: ... HEK293 cells and MCF7 cells purchased from ATCC were cultured in DMEM (high glucose, Life Technologies Corporation, 11965084) containing 15% fetal bovine serum ...
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bioRxiv - Cell Biology 2023Quote: Hek293T and Hek293 Flp-In T-REx cells were maintained without antibiotics in Dulbecco’s modified Eagle’s medium (DMEM) (Invitrogen) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Plant Biology 2023Quote: ... HEK293 cells were seeded and grown on poly-lysine-coated Lab-Tek II eight-well chamber slides (NUNC) overnight and transfected with plasmid DNA using the Lipofectamine™ 3000 Transfection System (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: Lentivirus was made by transfecting HEK293 cells with ViraPower packaging plasmids (pLP1, pLP2, and pLP/VSVG) from Invitrogen along with the viral vector using JetPrime ...
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bioRxiv - Cell Biology 2023Quote: Human embryonic kidney (HEK293) cells were obtained from ATCC and cultured in Dulbecco’s Modified Eagle’s medium (DMEM, Gibco) supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: Approximately 1 × 105 HEK293 cells were seeded in flat-bottom 24-well plates (Thermo Fisher Scientific or FALCON) and incubated overnight ...
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bioRxiv - Immunology 2023Quote: ... Plasmids were then transfected into human embryonic kidney cells (HEK293-E, National Research Council, Canada) using 293Fectin (Thermofisher). Six days post-transfection ...
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bioRxiv - Genomics 2023Quote: ... sgRNA-transduced HEK293-idCas9 cells were cultured in high glucose DMEM medium supplemented with L-Glutamine (Gibco 11965092) and 10% FBS ...
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bioRxiv - Neuroscience 2023Quote: Human embryonic kidney (HEK293) cells obtained from ATCC (Manassas, VA, USA) were cultured in Dulbecco’s Modified Eagle’s medium (DMEM, Gibco) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293S GnTI− cells (ATCC CRL-3022) were grown in suspension at 37 °C in Freestyle 293 medium (Gibco) supplemented with 2% (v/v ...
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bioRxiv - Biophysics 2024Quote: ... the HEK293 cells were incubated with 1 μM Alexa Fluor-647 conjugated α-bungarotoxin (BTX-647; Life Technologies) at 4°C for 1 hr ...
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Recruitment of the m6A/Am demethylase FTO to target RNAs by the telomeric zinc finger protein ZBTB48bioRxiv - Molecular Biology 2024Quote: The HEK293 Flp-In T-REx cell line was obtained from Life Technologies (Invitrogen catalogue number R780-07). HEK293 and HCT-116 cells (isolated from the colon of an adult male ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting plasmid was transfected into HEK293 cells at 70% confluency using Genejuice (Fisher Scientific 70-967-3) at a 1:3 ratio (DNA ...
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bioRxiv - Genomics 2024Quote: HEK293 were also cultured in Dulbecco’s Modified Eagle Medium 1X (41966-029) with 10% FBS (GIBCO, 10270-106) and 1% PIS in a 10 cm dish and maintained in an incubator at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2019Quote: ... total OT-I and reference (B6 or F5 Rag1-/-) thymocytes were stained with Cell Proliferation Dyes eFluor450 (1□M) or eFluor670 (0.5□M) (Thermo Fisher Scientific) at 107 cells/ml in PBS for 15 minutes at 37°C prior to overlay on thymic slices ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were extracted using lysis buffer (8 M urea, 2 M thiourea, 4% CHAPS, 40mM DTT) supplemented with Halt protease inhibitor complete cocktail (Thermo Fisher) by bead beating (50 Hz for 3 five-minute cycles ...
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bioRxiv - Molecular Biology 2019Quote: ... Negative mode MS analysis was performed in the full scan mode in the mass range of 200-1100 m/z (resolving power R=140000 at m/z 200) using an QExactive Plus mass spectrometer (ThermoFisher Scientific). MS parameters in the Tune software (version 2.5 Build 2042 ...
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bioRxiv - Physiology 2020Quote: ... Atp6ap1/Ac45 (Dharmacon cat. #M-060210-01-0005) or Atp6ap2/Prr (Dharmacon cat. #M-063641-02-0005) using Lipofectamine RNAiMax (Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... were covalently linked to the surface of M-450 Tosyl beads (Dynabead M-450, 140130, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2022Quote: Purified protein was exchanged into reaction buffer (0.5 M NaCl, 0.1 M Na3PO4, pH 7.5) and conjugated to DyLight-488 NHS ester (Thermo Fisher Scientific, Massachusetts, U.S.A.) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Protein pellets were resuspended in urea buffer (6 M urea, 2 M thiourea, 100 mM ammonium bicarbonate, pH 8.0) and quantified using EZQ (Invitrogen/Life Technologies).
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bioRxiv - Genomics 2023Quote: ... anti-FMRP and purchased anti-IgG (anti-rabbit IgG M-280 and anti-mouse IgG M-280 Dynabeads raised in sheep, Invitrogen, USA) were blocked to reduce background the night before the immunoprecipitation ...
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bioRxiv - Neuroscience 2023Quote: ... which include cell separation by gradient centrifugation and lymphocyte growth enhancement with 1% (v/v) of the mitogenic phytohemagglutinin-M (PHA-M, ThermoFisher 10576015).
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bioRxiv - Bioengineering 2019Quote: ... and cryostat section (10μm) were stained using the following antibodies: rat anti-IL2 (eBioscience 14-7029-85) and anti-rat AlexaFluor488 (Invitrogen A21208). For vascular staining goat anti-CD31 (R&D AF3628 ...
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bioRxiv - Bioengineering 2019Quote: Antigen expression was confirmed on ice-cold acetone fixed 8-μm cryostat sections of SKRC52 and CT26-CAIX stained with IL2-XE114-TNFmut and IL2-F8-TNFmut (final concentration 5μg/mL) and detected with rat anti-IL2 (eBioscience 14-7029-85) and anti-rat AlexaFluor488 (Invitrogen A21208). For vascular staining goat anti-CD31 (R&D AF3628 ...
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bioRxiv - Biophysics 2020Quote: Primary rat cortical neurons (RCN) prepared from the cortex of day-18 rat embryos (A10840-01, Life Technologies, Gaithersburg, MD) were suspended in a culture medium (21103-049 ...
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bioRxiv - Neuroscience 2022Quote: ... Serum TNF and IL-1β concentrations were determined by using a rat TNF alpha Uncoated ELISA and a rat IL-1β ELISA Kit (all from Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Macrophages were positively enriched from spleen single-cell suspensions using anti-F4/80 antibody (rat, Bioscience, Clone T45-2342)-coated Dynabeads (anti-rat IgG, Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... Cells were then washed and incubated with anti-rat Ab magnetic beads at 1 bead/target cell for 40 min at 4C (Dynabeads sheep anti-rat IgG, Invitrogen). Cell suspensions were stained with Abs specific for congenic markers ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed and incubated with anti-rat Ab magnetic beads at 1 bead/target cell for 40 min at 4C (Dynabeads sheep anti-rat IgG, Invitrogen). Cells were sorted into 3mL of complete media (RPMI 1640 ...
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bioRxiv - Developmental Biology 2023Quote: ... using rat-anti-BrdU (1:500, OBT0030 Accurate) and donkey-anti-rat Alexa Fluor-568-conjugated secondary antibody (1:1000, Invitrogen).
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bioRxiv - Developmental Biology 2021Quote: ... and goat anti-rat 568 (Invitrogen, cat #A11077) antibodies in a dark humidified chamber for one hour at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... and anti-rat conjugated to Alexa647 (Thermofisher, A21472). Alexa568 conjugated phalloidin (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... rat anti-mCherry (Thermo Fisher, M11217, 1:500), rabbit anti-Halotag (Promega ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and anti-rat Alexa 488 (Invitrogen, A-11006) (1 μg/mL) ...
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bioRxiv - Developmental Biology 2020Quote: ... and goat anti-rat 647 (#A-21247; Invitrogen). Nuclei were stained with DAPI (1:1000 ...
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bioRxiv - Developmental Biology 2020Quote: ... A goat α-rat Alexa488 secondary antibody (Invitrogen) was used at 1:1,000 in blocking solution and nuclei stained with DAPI.
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bioRxiv - Developmental Biology 2021Quote: ... goat anti-rat Alexa546 (1:500; Invitrogen A11081) and TRITC-phalloidin (1:100 ...
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bioRxiv - Molecular Biology 2020Quote: ... rat α-mCherry (Thermo Fisher Scientific, M11217, RRID:AB_2536611); rabbit α-MPP8 (Proteintech ...
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bioRxiv - Cell Biology 2022Quote: Rat monoclonal anti-Ki67 (Thermofisher, 14-5698-80)
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bioRxiv - Immunology 2019Quote: ... goat anti-rat Alexa Fluor 568 (Molecular Probes). Sections were stained with DAPI and mounted with Prolong Gold (Life Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... anti-rat 568 and anti-Human 647 (Invitrogen) were diluted 1:1000 in PBS-Triton X-100 0.1% with 10% FBS ...
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bioRxiv - Immunology 2019Quote: ... Alexa Fluor 647-anti-Rat IgG (ThermoFisher Scientific). Reagents ...
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bioRxiv - Neuroscience 2019Quote: ... Alexa488-conjugated anti-rat IgG (A-11006, Invitrogen), Alexa594-conjugated anti-rabbit IgG (A21207 ...
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bioRxiv - Developmental Biology 2019Quote: ... AlexaFluor-568 anti-rat (1/300; A11077, Invitrogen). Nuclei were counterstained with DAPI (1:10,000 ...