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Citations for Thermo Fisher :
101 - 150 of 10000+ citations for rno mir 125b 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... with appropriate primer sets on a QuantStudio 5 Real-Time PCR System (Applied Biosystems). Primers for qRT-PCR analysis of Alrs ...
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bioRxiv - Immunology 2019Quote: ... Taqman miR primers and fluorescent probes were from Life Technologies. Quantitative PCR conditions for the Stratagene Cycler were ...
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bioRxiv - Pathology 2023Quote: ... TaqMan MGB primer/probe sets and Eukaryotic 18S rRNA VIC primer/probe set endogenous control (Thermo Fisher) with Premix Ex Taq master mix (Takara ...
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bioRxiv - Pathology 2022Quote: ... qRT-PCR analyses were conducted utilizing the Centers for Disease Control 2019-nCoV N1 primers and probe (https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-panel-primer-probes.html) and AgPath-ID One-Step RT-PCR reagents (Ambion/ThermoFisher, Waltham, Massachusetts). We included a standard curve of serial dilutions of RNA extracted from SARS-CoV-2 virus stock (107 TCID50/ml ...
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bioRxiv - Cell Biology 2019Quote: ... at 20 nmol/L or control miRNA at 10 nmol/L plus either miR-181a-5p (10 nmol/L; Ambion Life Technologies ...
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bioRxiv - Molecular Biology 2022Quote: mirVana™ miRNA Mimics and Inhibitors were used for hsa-miR-181a-5p overexpression/inhibition (Ambion™ - 4464066 and 4464084). HeLa cells were transfected with corresponding mirVana™ miRNA in 24 well plates at a density of 20.000 cells per well ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitation of miR-423-5p expression levels was assessed using the Taqman™ MicroRNA Reverse Transcription kit (Thermo Fisher Scientific) and the specific Taqman™ MicroRNA Assay kit and the Universal PCR Master Mix No AmpErase UNG (both Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... and hsa-miR-485-5p (catalog#17100, PM10837) or with 50 pmol per well (24-well format) of miRNA miRVana inhibitors (Ambion, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... both shβ7 cells and H295R cells were transfected with control miRNA mimics or hsa-miR-139-5p mimics (Thermofisher, #MC11749) at a final concentration of 10nM ...
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bioRxiv - Cancer Biology 2024Quote: ... The miR-539-5p mimic or NC was cotransfected with the wild-type or mutant plasmid using lipofectamine 3000 (Invitrogen). Luciferase intensity was recorded 48 h after transfection using the dual-luciferase reporter assay system (Promega ...
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bioRxiv - Microbiology 2020Quote: ... qRT-PCR assays for miR-US5-1 and miR-UL112 were purchased from ThermoFisher Scientific.
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bioRxiv - Microbiology 2021Quote: ... 5µl were subjected to quantitative PCR using primer/probe sets for human Gapdh (Applied Biosystems), and EBOV trVLP 5’-trailer region ...
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bioRxiv - Immunology 2022Quote: ... and primer sets (Supplementary Table 1) on a StepOnePlus Real-Time PCR System (Applied Biosystems).
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bioRxiv - Developmental Biology 2019Quote: All primers were Assay-on-Demand primer/probe sets from ThermoFisher Scientific (Richlands ...
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bioRxiv - Cancer Biology 2020Quote: ... Primers for miR-147b and U6 were purchased from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... and target-specific primer/probe sets (ThermoFisher) or frozen at −80° C until use ...
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bioRxiv - Neuroscience 2021Quote: ... Primer/probe sets were obtained from ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... The following Taqman Primer/Probe sets (ThermoFisher) were used in this study ...
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bioRxiv - Molecular Biology 2020Quote: ... with Taqman assay primer sets (Applied Biosystems) as per the manufacturers specifications ...
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bioRxiv - Microbiology 2020Quote: ... using TaqMan primer/probe sets (Thermo Fisher) against ZIKV48 (Assay ID APH6AE9 ...
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bioRxiv - Immunology 2021Quote: ... Taqman primer/probe sets (Applied Biosystems, CA) were used for real-time gene expression analysis using ABI Prism 7500 ...
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bioRxiv - Cancer Biology 2020Quote: ... Primer-probe sets were purchased from ThermoFisher Scientific (Applied Biosystems Gene Expression Assays ...
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bioRxiv - Microbiology 2022Quote: ... and TaqMan primer-probe sets (ThermoFisher Scientific) listed in Table 3 on the CFX96 Touch Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Immunology 2024Quote: ... dNTP set and oligo(dT) primer (Invitrogen) per manufacturer’s protocols ...
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bioRxiv - Neuroscience 2023Quote: ... Primer/probe sets were obtained from ThermoFisher Scientific ...
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bioRxiv - Physiology 2022Quote: ... RT specific primers 5X (ThermoFisher) were multiplexed in a primer pool containing 1% of each diluted in an adequate volume of Tris-EDTA 1X ...
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bioRxiv - Cell Biology 2019Quote: ... at 20 nmol/L or control miRNA at 10 nmol/L plus either miR-181a-5p (10 nmol/L; Ambion Life Technologies; miRVana™ miRNA mimic ...
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bioRxiv - Cell Biology 2021Quote: ... Calu-3 cells were seeded at 2.5×105/ml with 100 or 300 nM of hsa-miR-145-5p miRNA precursor (PM11480, Ambion, Thermo Fisher Scientific). After 72 h ...
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bioRxiv - Genomics 2021Quote: ... Ten microliters of miR-199a-5p mimic or its negative control and miR-199a-5p inhibitor or its negative control (Genepharma, China) were dissolved in 100 μL opti-MEM(Gibco, USA). Then 100 μ L of this solution was gently mixed with 8 μ L of Lipofectamine™ 2000 (lipo ...
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bioRxiv - Neuroscience 2022Quote: ... We measured the abundance of miR-138-5p and the mRNAs of interest in a thermocycler ABI Prism 7900 fast (Applied Biosystems) for 40 cycles of two steps ...
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bioRxiv - Genetics 2023Quote: ... All data were analyzed using the Comparative ΔΔCT method to generate relative expression data using miR-192-5p (#A25576, Thermofisher, #478262_miR) as the internal control for miRNAs since its levels did not change significantly after of any of our experimental manipulations ...
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bioRxiv - Molecular Biology 2024Quote: ... Dharmacon) as a negative control or mmu-miR-7a-5p (C-310591-07-0002, Dharmacon) using Lipofectamine™ 2000 (11668030, Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: ... and gene specific primer sets on a StepOne Plus PCR Detection System (Applied Biosystems Life Technologies). Each qPCR reaction was carried out in triplicate ...
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bioRxiv - Developmental Biology 2019Quote: ... and gene specific primer sets on a StepOne Plus PCR Detection System (Applied Biosystems Life Technologies). Each qPCR reaction was carried out in triplicate ...
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bioRxiv - Molecular Biology 2021Quote: ... β-Actin DNA was PCR amplified using a premade TaqMan probe/primer set (ThermoFisher, Cat #431182), while β-Actin cDNA in RNA analyses was quantified using a separate Taqman probe/primer set that amplifies across a splice junction (FP ...
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bioRxiv - Microbiology 2023Quote: ... antarcticus cells was assessed by standard PCR with both sets of primers using DreamTaq polymerase (ThermoFisher) for 35 cycles at 55 °C annealing temperature ...
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bioRxiv - Developmental Biology 2020Quote: All primers were Assay-on-Demand primer/probe sets from Thermo Fisher Scientific (Richlands ...
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bioRxiv - Neuroscience 2019Quote: ... To perform PCR different primer sets were used (Tab.1) and PCR was executed with Phire Hot Start II DNA Polymerase (ThermoFisher).
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bioRxiv - Neuroscience 2022Quote: ... We used reverse-transcriptase-specific primers for mmu-miR-134 (Applied Biosystems miRNA assay ID 001186 ...
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Bat influenza vectored NS1-truncated live vaccine protects pigs against heterologous virus challengebioRxiv - Microbiology 2020Quote: ... Each gene segment was synthesized by gene specific primers (primers available upon request) using SuperScript III One-Step RT-PCR System (Invitrogen).
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bioRxiv - Cell Biology 2021Quote: ... Calu-3 cells were seeded at 2.5×105/ml with 100 or 300 nM of hsa-miR-145-5p miRNA precursor (PM11480, Ambion, Thermo Fisher Scientific). After 72 h ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 mg/kg body weight miR-182-5p-mimic or negative control was injected via the tail vein using Invivofectamine® 3.0 (Invitrogen, Carlsbad, US). Mice were phenotypically characterized by NMR on days -1 and 7 and a glucose tolerance test after 6 h fasting on day 5 ...
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bioRxiv - Molecular Biology 2021Quote: ... 20X Taqman primers/probe set (Thermo Fisher Scientific), 1µl of diluted cDNA and RNAse-free H2O ...
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bioRxiv - Neuroscience 2021Quote: ... The following primer/probe sets were used (ThermoFisher): Mouse ActB ...
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bioRxiv - Immunology 2022Quote: ... Optimized primer/probes sets were purchased from ThermoFisher Scientific with the following assay IDs ...
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bioRxiv - Immunology 2021Quote: ... using commercial Taqman primer/probe sets (Life Technologies). Probes for mouse genes included ...
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bioRxiv - Neuroscience 2023Quote: ... 20X TaqMan primers/probe set (Thermo Fisher Scientific), 1 µL of diluted cDNA and RNAse-free H2O ...
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bioRxiv - Microbiology 2020Quote: ... real-time PCR was performed using commercially validated TaqMan-based primer-probe sets (Supplementary Table 1) and TaqMan Universal PCR Mix (Life Technologies). Results were then normalized as described above.
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bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR (qRT-PCR) was conducted using TaqMan Assay-on-Demand primer sets (Applied Biosystems by Thermo Fisher Scientific) or Power SYBR Green PCR Master Mix (Merck) ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR (qRT-PCR) was conducted using TaqMan Assay-on-Demand primer sets (Applied Biosystems by Thermo Fisher Scientific) or Power SYBR Green PCR Master Mix (Merck) ...