Labshake search
Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations for Piggybac Transposable Element Derived 3 PGBD3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... 5 µg of total RNA from each ovary pool (field- and colony-derived) was used to generate cDNA with the RevertAid First Strand cDNA Synthesis kit (Thermo Scientific, Waltham, MA) following the manufacturer’s instructions for random hexamers ...
-
bioRxiv - Cell Biology 2021Quote: ... and a lentiviral backbone vector containing the construct of interest (derived from pHRSIN-CSGW, see key resource table) using lipofectamine LTX (Invitrogen, Catalog # 15338–100). The HEK293T media was harvested 60-72 hr post-transfection ...
-
bioRxiv - Biochemistry 2022Quote: ... The clarified supernatant was retrieved and added to pre-cleared beads derived from 50 µL of Dynabeads® MyOne Streptavidin C1 (Thermo Fisher Scientific). For the evaluation of biotin and water elution fractions ...
-
bioRxiv - Cell Biology 2021Quote: Stem cell-derived β-cells were dissociated from clusters and plated onto 35 mm glass-bottom dishes coated with 5% Matrigel (Fisher Scientific, 354277). Cells were washed twice with basal KRBH solution composed of (mM) ...
-
bioRxiv - Cancer Biology 2022Quote: ... hTERT immortalized adipose derived Mesenchymal stem cells were purchased from ATCC (SCRC-4000) and were cultured in MesenPRO RS™ Medium (Gibco, 12746-012) supplemented with L-glutamine (Sigma-Aldrich ...
-
bioRxiv - Bioengineering 2022Quote: Epidermal growth factor receptor (EGFR) protein expression levels on the surface of Gli36-derived EVs were quantified using an EGFR Human ELISA kit (ThermoFisher Scientific, Waltham, MA). EVs were spiked in healthy donor serum at different concentrations ranging from 0 to 1.0E11 particles/mL while maintaining the serum-derived EV concentration at 1.0E9 particles/mL ...
-
bioRxiv - Immunology 2023Quote: ... and BMDMs (bone marrow derived macrophages) obtained by differentiation in vitro in RPMI 1640 medium with L-glutamine (Gibco Thermo Fisher Scientific 21875034) supplemented with 10% heat-inactivated fetal bovine serum (Sigma F0392 ...
-
bioRxiv - Immunology 2023Quote: ... and BMDMs (bone marrow derived macrophages) obtained by differentiation in vitro in RPMI 1640 medium with L-glutamine (Gibco Thermo Fisher Scientific 21875034) supplemented with 10% heat-inactivated fetal bovine serum (Sigma F0392 ...
-
bioRxiv - Microbiology 2023Quote: ... All derived stable cells expressing selectable markers were grown in complete DMEM containing puromycin (Gibco; U-2 OS: 0.5μg/mL, Huh7.5: 4μg/mL), blasticidin (Gibco ...
-
bioRxiv - Immunology 2023Quote: ... the isolated CD14+ monocytes were differentiated into monocyte-derived DCs within 5 days and cultured in RPMI 1640 medium (Gibco Life Technologies, Gaithersburg, Md.) containing 10% FCS ...
-
bioRxiv - Cancer Biology 2023Quote: ... male derived) with constitutive expression of the Cas9 protein was grown in Roswell Park Memorial Institute medium 1640 GlutaMAX Supplement (ThermoFisher Scientific Waltham, Massachusetts) supplemented with 20% FCS ...
-
bioRxiv - Cell Biology 2023Quote: ... was derived from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and plated in RPMI-1640 medium (Thermo Fisher Scientific, USA) containing 8% fetal bovine serum (FBS ...
-
bioRxiv - Immunology 2024Quote: Total RNA was extracted from the co-culture of MSCs and aGvHD patients derived PBMNCs using the phenol-chloroform extraction method by TRIzolTM reagent (Thermo Fisher Scientific, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2024Quote: TC7 cells (a human cell line derived from Caco-2 colon adenocarcinoma cells 60) were grown in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, catalog number 21885-025) supplemented with 10% heat-inactivated serum ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... two rounds of cell selections on 5×106 mMOG expressing cells (in-house cells derived from Flp-InTM CHO Cell Line, Invitrogen, Waltham, MA, USA) were performed followed by one round of in solution selection with 50 nM in-house biotinylated human MOG (hMOG ...
-
bioRxiv - Molecular Biology 2024Quote: ... HTS libraries for CfMV and RYMV CP derived VLPs were prepared according to the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific, USA). HTS was performed on the Ion Torrent Proton (Thermo Fisher Scientific ...
-
bioRxiv - Synthetic Biology 2024Quote: NGN2 lines were generated following a previously described protocol.55 Human induced pluripotent stem cells (hiPSCs) were derived from dermal fibroblasts isolated from a healthy 32-year-old female human donor (Invitrogen, C-013-5C) using CytoTune™-iPS 2.0 Sendai Reprogramming Kit (Invitrogen ...
-
bioRxiv - Molecular Biology 2024Quote: High-throughput sequencing (HTS) libraries for RGMoV CP derived VLPs were prepared according to the Ion Total RNA-Seq Kit (Thermo Fisher Scientific, USA) protocol ...
-
bioRxiv - Bioengineering 2024Quote: ... The next day terminally differentiated hiPSC-derived podocytes were labeled with eBioscience Cell Proliferation Dye eFluor 450 (Invitrogen, final concentration of 2 µM) according to the manufacturer’s instruction and seeded onto the opposite side of the membrane ...
-
bioRxiv - Neuroscience 2022Quote: ... NIM was exchanged daily for 3:1-DMEM/F12-medium (3 parts DMEM (Thermo Fisher Scientific, #31966047) and one-part F12 medium (Ham’s F-12 Nutrient Mix ...
-
bioRxiv - Cell Biology 2021Quote: ... 1–2 mg of VLDL were combined with 100 μL of 3 mg/mL 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine perchlorate (DiD; Invitrogen) in DMSO and then re-isolated by ultracentrifugation ...
-
bioRxiv - Microbiology 2021Quote: ... 50 nM siRNA (IMPDH2 assay ID: s7417, sense: 5’-CCAAGAAAAUCACUCUUtt-3’; anti-sense: 5’-UUAAGAGUGAUUUUCUUGGtc-3’, Ambion by Life technologies ...
-
bioRxiv - Genetics 2022Quote: ... mcherry: 5’-ATGGTGAGCAAGGGCGAGGAG-3’ and 5’-CTTACTTGTACAGCTCGTCCATG-3’) from pKHR8-foxc1aKI and separately subcloned into pJET2.1 (ThermoFisher) to give pJET-mVenus and -mCherry ...
-
bioRxiv - Biophysics 2022Quote: ... 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate (DiI) and (ii) 1,1’-Dioctadecyl-3,3,3’,3’-tetramethylindotricarbocyanine iodide (DiR) were both from Invitrogen. The standard lipids (i ...
-
bioRxiv - Neuroscience 2024Quote: ... The siRNA sequence for YTHDF2 is 5′-AAGGACGTTCCCAATAGCCAA-3′ and for HIF1α is 5’-GCTGATTTGTGAACCCAT-3’ (Ambion). After 48 h from the initial transfection ...
-
bioRxiv - Immunology 2023Quote: ... The caspase-3/7 activity assay contained CellEvent Caspase- 3/7 Green Detection Reagent (Thermo Fisher, C10723) at a final ...
-
bioRxiv - Biochemistry 2023Quote: ... tRNAGln was 3’ biotin labeled with the Pierce ™ RNA 3’ end biotin labeling kit (Thermo Fisher) according to manufacturer’s recommendations ...
-
bioRxiv - Cell Biology 2022Quote: ... and washed 3 times with fresh ECGM and 3 times with ECGM/Hoechst 33342 (NucBlue™ ThermoFisher). After 10min incubation ...
-
bioRxiv - Biochemistry 2023Quote: ... 3 mM DTT) and homogenized using a Dounce homogenizer followed by DNaseІ treatment (3 U/µl, ThermoFisher) for one hour on ice ...
-
bioRxiv - Cancer Biology 2024Quote: ... UM-UC-3 cells were fluorescently labeled using 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate (DiI, ThermoFisher, #D3899) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2024Quote: ... forward and reverse primers (800 nM; BLVpol_5f: 5′-CCTCAATTCCCTTTAAACTA-3′; BLVpol_3r: 5′-GTACCGGGAAGACTGGATTA-3′; Thermo Fisher Scientific), and 200 ng of gDNA template ...
-
bioRxiv - Neuroscience 2023Quote: The brains of P9-11 C57 mice were immersed and fixed for 3 days in cold 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide HCl (EDC, Thermo Fisher Scientific, in 0.1 M PB, pH 7.4) at 4°C ...
-
bioRxiv - Biochemistry 2024Quote: ... dsRNA complex (2:1 molar ratio) were cross-linked with 30 μg 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, Thermo Fisher Scientific, EDC: protein = 3:1 w/w) in the presence of 66 μg N-hydroxysulphosuccinimide (NHS ...
-
bioRxiv - Cancer Biology 2020Quote: ... cells were washed 3 times for 8 minutes each and then placed in the secondary antibodies conjugated to either Cy3 or FITC (Thermo Fisher Scientific, Waltham, MA, USA) at a dilution of 1:1000 in 1% BSA for 2 hours ...
-
bioRxiv - Neuroscience 2023Quote: ... slides were washed and incubated 3 h with secondary antibodies: Alexa Fluor 488 (Anti-rabbit, 1:1000, Invitrogen, Themo Fisher Scientific, Slangerup Denmark, #A11008, AB_143165), Cy3 (monoclonal donkey anti-rabbit 1:600 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Knockdown of the protein was monitored by western blotting against the V5×3 epitope using α-V5 mouse primary antibody (Invitrogen / Thermo Fisher Scientific – 1:1000). Oligonucleotides used for PCR amplification are given in Table S4.
-
bioRxiv - Biochemistry 2023Quote: ... washed in 1xPBS for 3×10 minutes followed by secondary antibody Alexa Fluor-conjugated 488 goat anti-mouse IgM (1:500; Invitrogen Thermo Fisher Scientific, Waltham, MA) for 40 minutes in the dark ...
-
bioRxiv - Developmental Biology 2024Quote: ... Cells were washed 3 times with PBS-T and then incubated with AlexaFluor 488- or 594-conjugated secondary antibodies raised in donkey (Thermo Fisher, See Key Resources Table) at a 1:500 dilution in 3% donkey serum in PBS.
-
bioRxiv - Cell Biology 2024Quote: ... Concentrations of Fe and Zn were determined using inductively coupled plasma-sector field mass spectrometry ICP-SFMS (ELEMENT XR, Thermo Fisher Scientific Inc., Bremen, Germany). 56Fe and 66Zn were measured using medium resolution mode (R = 4,000) ...
-
bioRxiv - Bioengineering 2020Quote: eNVs were isolated from human bone marrow-derived MSCs obtained from the American Type Culture Collection (ATCC PCS-500-012) and cultured with MesenPRO RS™ Medium (Gibco™, Gaithersburg, MD) (14-17) ...
-
bioRxiv - Pathology 2021Quote: ... and proximal tubular cell line derived from normal kidney (HK-2, ATCC® CRL-2190™) were cultured with 1:1 DMEM/F12 (Gibco 11320-033) supplemented with 10% FBS ...
-
bioRxiv - Cancer Biology 2020Quote: hPSC-derived NPCs at passage 0-2 were harvested with Accutase and plated on PLO/Laminin-coated glass coverslips (Thermo Fisher, #12-545-101) at a density of 10,000-20,000 cells/cm2 ...
-
bioRxiv - Cancer Biology 2020Quote: DIPG and pGBM patient-derived cultures established in our lab and others were grown in stem cell media consisting of 250ml DMEM/F12 (Thermo Fisher Scientific, 11330-038), 250ml Neurobasal-A Medium (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2020Quote: Bone marrow-derived dendritic cells (BMDCs) were generated by culturing murine bone marrow cell suspensions in RPMI 1640 medium (Gibco, via Thermo Fisher Scientific) supplemented with 10% FBS ...
-
bioRxiv - Cancer Biology 2020Quote: ... PDOX-derived single cell tumor cells and cell lines were stained with IR-LIVE/DEAD® Fixable Dead Cell Stains (Invitrogen; 1 μg/ml) and fixed with cold 80% ethanol ...
-
bioRxiv - Neuroscience 2022Quote: ... Clonal lines D2-c1-iPSC and D2-c2-iPSC were derived from the commercially available dermal fibroblasts from a female donor (Gibco™, Lot number: 181388). The skin biopsy was sampled at the age of 34 ...
-
bioRxiv - Cell Biology 2022Quote: Raji and Daudi cell lines derived from Burkitt’s lymphoma were routinely grown in advanced Roswell Park Memorial Institute 1640 medium (Gibco®, Life Technologies, Carlsbad, CA, EUA) supplemented with 10% fetal bovine serum ...
-
bioRxiv - Cell Biology 2022Quote: LC-MS/MS analysis to assess differences in protein content between percoll gradient-enriched mitochondria derived from K562 wild-type or MTCH2 depleted cells was performed with an EASY-nLC 1200 (ThermoFisher Scientific, San Jose, CA) coupled to an Orbitrap Eclipse Tribrid mass spectrometer (ThermoFisher Scientific ...
-
bioRxiv - Immunology 2023Quote: ... the isolated CD14+ monocytes were differentiated into monocyte-derived DCs within 5 days and cultured in RPMI 1640 medium (Gibco Life Technologies, Gaithersburg, Md.) containing 10% FCS ...
-
bioRxiv - Neuroscience 2024Quote: Nuclear and cytoplasmic fractions were isolated from human iPSC-derived cortical neurons using the NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific cat. no. 78833). Cells were harvested via centrifugation at 500 × g for 5 minutes and washed with PBS ...