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Citations for Thermo Fisher :
1201 - 1250 of 10000+ citations for Piggybac Transposable Element Derived 3 PGBD3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ROS production from CD8+ T-cells following MDSC-derived exosomes treatment in vitro was estimated by labeling the CD8+ T-cells using CM-H2DCFDA (Invitrogen™, C6827). In short CD8+ T-cells were isolated according to the above-mentioned method ...
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bioRxiv - Bioengineering 2021Quote: ... episomally derived from human foreskin fibroblasts) was cultured on tissue culture-treated plasticware pre-coated with 1mL per well Geltrex (Life Technologies A1413302) for 1 hour at 37C ...
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bioRxiv - Neuroscience 2022Quote: ... The concentration and purity of DNA derived from exosomes was assessed by measurement of absorbance and 260/280 ratio with a NanoDrop 2000/c (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2021Quote: ... The amplified fragments derived from the mutants were sequenced using the BigDye Terminatorv3.1 Cycle Sequencing Kit and 3130xl Genetic Analyzer (Thermo Fisher Scientific, USA). Homozygous mutant plants lacking the CRISPR/Cas9 expression cassette were isolated by self-crossing.
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bioRxiv - Developmental Biology 2021Quote: ... single cell-derived mESC colonies were picked and divided into three culture conditions: (1) with G418 (Geneticin; Cat. 10131027, Thermo Fisher Scientific), (2 ...
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bioRxiv - Cell Biology 2020Quote: FAPs-derived EVs at a final concentration of 10 μg in 20 μL of PBS1x (0.5ug/μl) (#14190-1444, Gibco by life technologies) and its vehicle of control (20 µl PBS1x ...
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bioRxiv - Neuroscience 2020Quote: ... sequences were amplified from plasmids derived from ser-2(2)p::FRT::YFP,andclonedupstreamof FRT::STOP::FRT::GCaMP6s to generate WY016 using Gateway (Thermo Fisher Scientific). To generate PSAB1206 ...
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bioRxiv - Cancer Biology 2022Quote: ... All melanoma cell lines and patient-derived primary melanoma cells were maintained in Roswell Park Memorial Institute (RPMI) medium (Thermo Fisher Scientific) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2022Quote: mRNAs were extracted from neurons derived from AD or Control hiPSCs with or without SARS-CoV-2 infection in Trizol reagent (Thermofisher, Waltham, MA) and followed by cDNA synthesis with SuperScript™ III Reverse Transcriptase kit (ThermoFisher) ...
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bioRxiv - Cell Biology 2020Quote: Bone marrow-derived dendritic cells (BMDCs) were generated by culturing murine bone marrow cell suspensions in RPMI 1640 medium (Gibco, via Thermo Fisher Scientific) supplemented with 10% FBS ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... half the wells exposed to particulate and half the control wells were apically infected with SARS-CoV-2 derived from clinical isolate WA1 (20) in high glucose DMEM (Gibco 11995-065) with 5% heat-inactivated fetal bovine serum ...
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bioRxiv - Genetics 2021Quote: DNA concentration of the blood-derived DNA was quantified with Qubit fluorometric quantification using the dsDNA BR Assay kit (Thermo Fisher Scientific). Library preparation of 1.5 μg input DNA from the six DNA samples was performed with the ligation sequencing kit (SQK-LSK109 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... pHW191 encoding the PR8-derived PB2 gene (60) was used as a template for PCR amplification (Phusion Hot Start II DNA polymerase, Thermo Fisher, #F549L). Here ...
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bioRxiv - Cancer Biology 2021Quote: ... 100 µL of exosome- or MV-fractions derived from 4T1 cells expressing PalmReNL were stained with CellTrace Violet (CTV; Thermo Fisher, C34571), followed by removing free dye with a spin desalting column (BioVision ...
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bioRxiv - Cell Biology 2023Quote: ... Procyclic cells (PCF) derived from Trypanosoma brucei subspecies brucei strain Lister 427 were grown in SDM-79 JRH 57453 media (Life Technologies UK) supplemented with 7.5 mg/l of hemin (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: Insect cell derived recombinant tag-less eIF2A was expressed in ExpiSf9 cells using the ExpiSf Expression System Starter Kit (Thermo Fisher # A38841) following the manufacturer’s protocol and adapting the IMPACT (Intein Mediated Purification with an Affinity Chitin-binding Tag ...
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bioRxiv - Systems Biology 2023Quote: Acquisition of single-cell derived peptides was conducted with an Orbitrap Astral mass spectrometer operated in positive mode with the FAIMS Pro interface (Thermo Fisher Scientific) using a compensation voltage set to −50 V ...
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bioRxiv - Systems Biology 2023Quote: ... EYA4-APEX samples and μOR-APEX samples derived from SH-SY5Y cells were analyzed on an Orbitrap Exploris 480 mass spectrometry system (Thermo Fisher Scientific) coupled to a Easy nLC 1200 nano-flow ultra high-pressure liquid chromatography (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... and MCF7 parental and transfection derived cell lines were cultured in Minimum Essential Medium-alpha 1X (MEM-α 1X) (Gibco; Life technologies), supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... and MCF7 parental and transfection derived cell lines were cultured in Minimum Essential Medium-alpha 1X (MEM-α 1X) (Gibco; Life technologies), supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2022Quote: ... and 1X concentration OPP reaction buffer (the last two reagents derived from the Click-iT™ Plus OPP Alexa Fluor™ 488 Protein Synthesis Assay Kit (Invitrogen) and at concentrations relative to those recommended in the manufacturer’s instructions) ...
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bioRxiv - Biochemistry 2024Quote: ... and cloned into a pRK5-derived plasmid with a C- or N-terminal FLAG tag using the gateway vector conversion system (Invitrogen, cat # 11828029). All gene constructs were verified by DNA sequencing.
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bioRxiv - Bioengineering 2023Quote: ... were derived from a proprietary library of 8-kb synthetic DNA chunks deposited in a GeneArt plasmid (ordered from Thermo Fisher Scientific) that was custom designed for construction of yeast synthetic chromosomes (unpublished).
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bioRxiv - Cell Biology 2023Quote: between 0.2·106-1·106 of VS derived cells were reprogrammed using the CytoTune™-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher Scientific), a non-integrative cell reprogramming method holding the four Yamanaka factors (OCT4 ...
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bioRxiv - Plant Biology 2022Quote: ... Enzymatic activity was measured via the derived products generated from a 4-MUG substrate (M1404, Duchefa) with a Fluoroskan Ascent II (Thermo Fisher Scientific). GUS activity is presented in an arbitrary unit as the ratio between fluorescence data per minute and protein concentration.
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bioRxiv - Plant Biology 2022Quote: ... The amplicon was introduced into between the attR1 and attR2 sites of PUB-GWS-GFP binary vector (derived from pCAMBIA 1300) from pENTR/D-TOPO by Gateway LR recombination (Invitrogen, http://www.invitrogen.com/). The recombinant binary vector was transformed into Agrobacterium rhizogenes LBA1334 by standard procedure as described below.
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bioRxiv - Cancer Biology 2023Quote: ... were seeded either alone or with TNBC-derived CAFs at a 1:1 ratio on low adherence 96-well round bottom plates (Thermo Fisher # 174925), in DMEM+10%FBS media ...
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bioRxiv - Cell Biology 2022Quote: ... The H2-derived scFv was designed based on the amino acid sequences of the variable regions and synthesized by GeneArt (Thermo Fisher Scientific). The plasmid encoding H2 IgG is available from Addgene ...
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bioRxiv - Cell Biology 2023Quote: ... and referred to as EL4 in the paper) and gene edited lines (derived from this parental line) were grown in RPMI (Thermo Fisher Scientific) with 10% FBS (Biozol ...
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bioRxiv - Cancer Biology 2023Quote: FLC-H cells were generated from a patient-derived xenograft model (Oikawa et al., 2015) and grown in RPMI1640 media containing 300 mg/L l-glutamine (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... Enzymatic activity was measured via the derived products generated from a 4-MUG substrate with a Fluoroskan Ascent II (Thermo Fisher Scientific). GUS activity is presented in an arbitrary unit as the ratio between fluorescence data par minute and protein concentration.
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bioRxiv - Neuroscience 2024Quote: ... containing the indicated amounts of recombinant or brain-derived assemblies in complex with 1 µl of lipofectamine 2000 (Thermo Fisher Scientific, # 11668019). Cells were incubated at 37 °C for 1 h and the lipofectamine-mediated delivery of the assemblies were stopped by the addition of 100 µl DMEM containing 10% FBS ...
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bioRxiv - Biochemistry 2024Quote: ... Expi293F cells (derived from HEK293 cells) were grown in suspension and shaken at 125 rpm using serum-free Expi293TM expression medium (Gibco, Cat#A1435101) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2024Quote: Human monocyte-derived macrophages (hMDM) were cultured in Nunc™ MicroWell™ 96-well optical-bottom plates (Thermo Fisher Scientific, Waltham, MA) and left untreated or treated with vehicle (sterile 1X PBS) ...
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bioRxiv - Immunology 2024Quote: ... Two micrograms of non-hemolymph-derived or 200ng of hemolymph-derived total RNA were used for first-strand synthesis with the RevertAid reverse transcriptase kit (Thermo Fisher Scientific). Gene expression analysis was performed with quantitative real-time PCR (qPCR ...
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bioRxiv - Physiology 2024Quote: Total RNA was extracted from cryo-pulverized liver tissues and hiPSC-derived hepatocyte-like cells using Trizol (Cat #15596026, Thermo Fisher Scientific) and 1 ug RNA was reverse-transcribed using SuperScriptTM IV VILOTM Master Mix (Cat #11756050 ...
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bioRxiv - Immunology 2024Quote: ... Irradiated recipients received retroorbital injection of 5 x 106 donor-derived BMCs and 1 x 106 T cells in serum-free RPMI (Invitrogen, Carlsbad, CA).
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bioRxiv - Microbiology 2024Quote: ... derived from kidney of an adult African green monkey (Cercopithecus aethiops) were cultured in Minimum Essential Medium (MEM) (Gibco/Invitrogen, CA, USA) supplemented with 10% (v/v ...
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bioRxiv - Biochemistry 2024Quote: Acquisition of single-cell derived peptides was conducted with an Orbitrap Astral mass spectrometer operated in positive mode with the FAIMS Pro interface (Thermo Fisher Scientific) using a compensation voltage set to -48 V ...
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bioRxiv - Biochemistry 2024Quote: ... an unnecessary 18-amino-acid sequence (LESTSLYKKAGSAAAPFT) derived from pDEST17 and the Gateway entry vector pENTR/D-TOPO (Invitrogen, Carlsbad, CA, USA) was removed using PCR with the KOD One PCR Master Mix (TOYOBO Co. ...
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bioRxiv - Bioengineering 2024Quote: ... A total of 24 × 105 NHDFs and 30 × 105 HUVECs were resuspended in 2.4 ml of 1.25 mg ml-1 bovine dermis-derived native collagen solution (KOKEN CO. Ltd., Tokyo, Japan) that was mixed with 1× DMEM (Thermo Fisher Scientific), 15 mM HEPES (Dojindo ...
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bioRxiv - Cell Biology 2024Quote: ... [35] was used to isolate adipose tissue-derived mesenchymal stem cells (ASCs) and subcultured in low-glucose Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco BRL, Rockville, MD, USA) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... Synaptosomes (SYNs) were then extracted from mature iPSC-derived cortical neurons using Syn-PER™ Synaptic Protein Extraction Reagent (Thermo Fisher Scientific). 1x of protease inhibitors (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... hTERT-immortalized human primary cell lines: RPE-1 derived cell lines were grown in in DMEM: F12 without phenol red (Gibco 21041-025) supplemented with 10% FBS ...
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Turanose induced WOX5 restores symbiosis in the Medicago truncatula cytokinin perception mutant cre1bioRxiv - Plant Biology 2020Quote: ... and (MtWOX5) 5’-CACCATGCAGACGGTCCGAGATCTGTC-3’ and 5’- CCTTCGCTTAAGTTTCATGTAA-3’ (AhWOX5) and cloned into pENTR-dTOPO (Invitrogen). The entry clones of AhWOX5 & MtWOX5 recombined through LR clonase Gateway technology (Invitrogen ...
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Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responsesbioRxiv - Molecular Biology 2020Quote: ... The eight rice 14-3-3 genes cloned in the yeast two-hybrid vector pDEST22 (Invitrogen) were used from a previous study (Deb et al. ...
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bioRxiv - Cell Biology 2021Quote: ... N-hydroxysulfosuccinimide (sulfo-NHS) and 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) from Thermo Scientific were dissolved in 18 MOhm DDW immediately before use ...
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bioRxiv - Bioengineering 2022Quote: ... 1-ethyl-3-(−3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) was purchased from Fisher Scientific (Pittsburgh, PA, USA). 2-Bromo-2-methylpropionic acid (BMPA) ...
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bioRxiv - Cancer Biology 2022Quote: ... slides were incubated for 24 minutes in a mixture of 3% 3-Aminopropyltriethoxysilane (ACROS Organics, 430941000) and 5% acetic acid in HPLC EtOH ...
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bioRxiv - Immunology 2020Quote: ... 3 μL RT reaction mix consisting of 3 μL of 50 μM random hexamers (Thermo Fisher), 0.8 μL of 25 mM deoxyribonucleotide triphosphates (dNTPs ...