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Citations for Thermo Fisher :
1401 - 1450 of 3690 citations for CdSeTe ZnS Quantum Dots NIR region Water solvent 760nm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... zipper tag via a 5XGGS linker region was co-transfected with N-terminally His-tagged human Leptin in suspension HEK293 FreeStyle cells (ThermoFisher) in the presence of 20 μM kifunensine (Dextra ...
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bioRxiv - Microbiology 2023Quote: BC pellicles were measured for overall light transmission in the visible light region using a UV-Vis spectrophotometer (Thermo Scientific Evolution 300 UV-Visible Spectrophotometer ...
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bioRxiv - Neuroscience 2023Quote: ... Primer/probes (designed against a non-coding region in the construct) was used with TaqMan® Master Mix (Applied Biosystems).
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bioRxiv - Neuroscience 2023Quote: ... The blot has been cut probing different regions of the same blot with the following primary antibodies: rabbit anti-Asic1a (1:1000, Invitrogen), rabbit anti-GluA1 (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... The nucleotide sequence of the internal region was determined by BigDye® Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, USA) and ABI PRISM®3100 Genetic Analyzer (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... A 11.2 kb PCR product that included a region from CLN3 exon 3 to exon 9 was amplified using a high fidelity polymerase (Platinum Taq High Fidelity, Invitrogen) and CLN3 primers pCLN3F3 and pCLN3R3 (Supplemental Table 2) ...
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bioRxiv - Neuroscience 2022Quote: ... The ipsilateral cortex and hippocampus (and corresponding regions in naïve animals) were microdissected on ice and immediately homogenized by pipetting and vortexing in TRIzol (Thermo Fisher). Total RNA was extracted using manufacturer instructions and tested for purity (by measuring A260/A280 and A260/A230 ratios via a Nanodrop 1000 Thermo Fisher ...
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bioRxiv - Zoology 2022Quote: ... amino acid regions likely to contain immunogenic sites were identified and submitted to the Thermofisher Life Sciences custom antibodies production service (Thermofisher Life Sciences ...
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bioRxiv - Evolutionary Biology 2023Quote: The putative regulatory regions were cloned after PCR amplification on genomic DNA in the PCR8/GW/TOPO vector (Life Technologies). Using Gateway technology (Life Technologies) ...
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bioRxiv - Plant Biology 2024Quote: ... the promoter regions upstream of the start codon of DGK1 (2472 bp) and DGK2 (1438 bp) were inserted via BP reactions (Invitrogen) into a pDONR R4-L1 vector ...
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bioRxiv - Microbiology 2023Quote: ... was confirmed by PCR amplification of the genomic region and by TOPO cloning into pCRII-Blunt-TOPO (Cat# K280002, ThermoFisher), per the manufacturer’s instructions (TOPO primers FWD ...
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bioRxiv - Microbiology 2023Quote: ... Viral RNA producing cells were detected by labelling HIV-1 unspliced mRNA with a set of 40 probe pairs against the GagPol region (Affymetrix eBioscience catalogue number GagPol HIV-1 VF10-10884 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µL of the reverse transcription reaction was used to amplify regions of interest using gene-specific primers in a 50 µL PCR reaction using recombinant Taq DNA Polymerase (Invitrogen) (see Table S2) ...
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bioRxiv - Plant Biology 2023Quote: Full-length coding region of AtIPK2α (∼900bp) was cloned into pOpOff2(Hyg)(Parvin et al., 2017) by Gateway recombination (LR ClonaseII, Invitrogen). The resulting vector pOpOFF2(Hyg)-AtIPK2α was used to stably transform the atipk2β knockout plants using Agrobacterium tumefaciens GV3101-mediated transformation by floral dipping method as described previously (Laha et al. ...
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bioRxiv - Bioengineering 2024Quote: ... was performed using custom primers for 16S rRNA gene (V1-V3 regions)26 (27F:AGAGTTTGATCMTGGCTCAG; 518R:GTATTACCGCGGCTGCTGG) (Thermo-Fisher Scientific Waltham, MA). Master mix (Thermo-Fisher Scientific Waltham ...
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bioRxiv - Molecular Biology 2023Quote: ... primers with upstream attB-regions suitable for BP-clonase-recombination (attB1: 5′-GGGGACAAGTTTGTACAAAAAAGCAGGCTTAACA-3′; attB2: 5′-GGGGACCACTTTGTACAAGAAAGCTGGGT-3′, Gateway Technology, Invitrogen). By same the method ...
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bioRxiv - Plant Biology 2022Quote: ... pUQ10B::RLP4/4-L1-ECD:RFP and pUB::RLP4/4-L1ΔID:RFP were all generated by cloning the relevant gDNA region into pDONR207 (Invitrogen/Thermo Fisher Scientific) using Gateway™ BP Clonase II Enzyme Mix (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: A 5.2 kb genomic fragment of AAR2 containing the promoter and coding region without the stop codon was PCR-amplified and cloned into pENTR/D-TOPO (Invitrogen) and then introduced into pGWB40 (60 ...
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bioRxiv - Plant Biology 2022Quote: The ZmEREB57 or ZmAOC2 coding region was cloned into the pBI121-GFP vector by means of a Gateway LR reaction (Invitrogen, the gene-specific primers listed in Supplemental Table S8 ...
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bioRxiv - Plant Biology 2022Quote: The pZPR3:3xNLS-RFP reporter was created by first cloning the 3.2kb region upstream of the ZPR3 start codon into pCR8-GW (Invitrogen; NEB), followed by an LR reaction using a modified pGREEN binary vector with a Gateway cassette upstream of 3xNLS-RFP (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: Total RNA isolation from the AAV9 virus injected region of the left frontal cortex was performed as previously described [40] by TRIzol LS Reagent (10296010, Invitrogen). For cDNA synthesis ...
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bioRxiv - Genomics 2022Quote: ... Using the DNA we again confirmed strain identity by sequencing the ITS region and nucleic acid quantity was measured using Qubit (ThermoFisher) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2023Quote: ... genomic DNA fragments including the promoter region were amplified and cloned into entry vectors (pENTR-D or PCR8 from Invitrogen) and cloned into destination vectors with C-terminal 3xFLAG (pEG302_GW_3xFLAG) ...
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bioRxiv - Plant Biology 2023Quote: A DNA fragment containing the full-length MtNCC1 genomic region and the MtNCC1p was cloned into the pGWB13 (Nakagawa et al., 2007) by Gateway technology (Invitrogen). This vector fuses three HA epitopes in the C-terminus of the protein ...
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bioRxiv - Physiology 2023Quote: ... followed by C-terminal region of human FGF-21 (amino acids 166-209) into a vector pCEP4 (Thermo Fisher Scientific). The construct was then transiently transfected to Expi293F cells (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... The FOXM1/RHNO1 promoter region was amplified with iMax-II DNA Polymerase (INtRON) and the products cloned into the pCR8/GW/TOPO vector (Invitrogen). Primers used are listed in Supplementary Table 9 ...
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bioRxiv - Plant Biology 2024Quote: All fragments including the CENPC-k and CENPC motifs with and without DNA-binding regions (Supplementary Table 1-4) were amplified from the αKNL2 and CENP-C cDNA clones in pDONR221 (Invitrogen) vector using primers listed in Supplementary Table 5 ...
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bioRxiv - Plant Biology 2024Quote: ... and C-terminal region (249-613 aa) were amplified from cDNA with or without STOP codon and cloned into pENTR2B (Invitrogen) or pENTR/D-TOPO (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... The cDNA corresponding to the regions coding for E2 and E1 was obtained by RT-PCR using SuperScript III reverse transcriptase (Invitrogen) and primers 137 (5’-CGTTTGTAGATAACTGCGG-3’ ...
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bioRxiv - Microbiology 2024Quote: ... and the reported variable regions of antibody CP33-H/L-LA22 (Tachibana et al., 2003) were codon-optimised for expression in mammalian cells (Invitrogen). Genomic fragments were amplified by PCR using PCR mastermix 2X (#K0171 ...
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bioRxiv - Neuroscience 2024Quote: ... Six micro-punches of each region were obtained from each mouse were respectively collected and immediately placed in 200 μl Trizol reagent (Invitrogen) and stored at −80 °C until analysis.
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bioRxiv - Molecular Biology 2024Quote: ... U95551.1) located in precure/core region (64) using RevertAidTM First Strand DNA Synthesis Kit (Thermo Fisher Scientific, Waltham, MA, USA). For absolute quantification ...
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bioRxiv - Plant Biology 2024Quote: ... The flanking regions and Hygromycin-trpC resistance cassette were amplified using Phusion® High-Fidelity DNA Polymerase (Thermo Fisher Scientific) and successive PCR product purification.
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bioRxiv - Neuroscience 2024Quote: A 5023bp promoter region upstream of calca start codon was cloned into pCR™8/GW/TOPO™ (Invitrogen™) using the following primers:
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bioRxiv - Neuroscience 2024Quote: A 1679bp promoter region upstream of adcyap1b start codon was cloned into pCR™8/GW/ TOPO™ (Invitrogen™) using the following primers:
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bioRxiv - Plant Biology 2024Quote: ... we first cloned the AtADF8 regulatory region from - 750 bp to +427 bp relative to the TSS into the gateway-compatible pENTR-D/TOPO plasmid (Invitrogen). This fragment consisted of the promoter ...
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bioRxiv - Biochemistry 2024Quote: ... the entire EGF domain and the flanking region modified by mucin-type O-glycans (amino acid position 18-302) were inserted into the pSeqTag2C-MycHis6 vector (Invitrogen) at the SfiI/NotI sites ...
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bioRxiv - Bioengineering 2024Quote: ... a region of interest containing valves was recorded at 28 frames/s using an EVOS M7000 microscope (Thermo Fisher Scientific) using a 10X/0.30NA objective ...
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bioRxiv - Bioengineering 2024Quote: Primers were designed to amplify a 500 – 800 bp region surrounding the edit site and target amplification was performed using Platinum Superfi II Master Mix (12368010, Invitrogen) using manufacturer recommended protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... ChIP efficiency was validated by qPCR for each antibody on specific genomic regions using powerSYBR Green Master Mix (Applied Biosystems) and compared for each primer pair to the input DNA ...
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bioRxiv - Microbiology 2021Quote: ... The RNA pellet was resuspended in 40 µl nuclease-free water (Invitrogen). Library preparation ...
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bioRxiv - Biophysics 2020Quote: ... The calibration sample consists of a water-covered glass coverslip (Fisher Scientific) with 40 nm fluorescent beads adhered to the surface (FluoSpheres (580/605) ...
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bioRxiv - Microbiology 2020Quote: ... Samples were eluted in 50 μL Ambion RNAse-free water (ThermoFisher, AM9937).
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bioRxiv - Systems Biology 2020Quote: Peptides were dissolved in water and TMT labels (ThermoFisher TMT10plex, TMT11-131C) (dissolved in acetonitrile ...
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bioRxiv - Cell Biology 2020Quote: ... 0.3% NP-40 in water) with 2% Proteinase K (Invitrogen Cat # 25530049). Lysis reactions were incubated at 55°C for 4 hours ...
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bioRxiv - Developmental Biology 2020Quote: ... then diluted 1:10 in UltraPure DNase/RNase-Free Distilled Water (ThermoFisher). RT-qPCR was performed using iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Genomics 2020Quote: ... or trl dsRNA suspended in 3mL of Invitrogen UltraPure water (ThermoFisher Scientific). Cells were serum starved for 45 minutes at room temperature ...
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bioRxiv - Genomics 2020Quote: ... Following a 70% ethanol wash and reconstituting in nucleotide-free water (Gibco) we treated the DNA samples with RNase (Thermo Scientific).
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bioRxiv - Genetics 2021Quote: ... and diluted in PCR-grade Ultrapure distilled water (Thermo Fisher Scientific 10977015). After dilution ...
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bioRxiv - Genomics 2021Quote: ... BLT beads were diluted 1:50 with nuclease free water (Invitrogen, USA). Ten nanograms of input gDNA in 10 ul ultrapure water (Invitrogen ...