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Citations for Thermo Fisher :
1351 - 1400 of 3690 citations for CdSeTe ZnS Quantum Dots NIR region Water solvent 760nm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... the purified PCR reaction was eluted with Nuclease-Free Water (Invitrogen). The PCR product was quantified from agarose gel ...
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bioRxiv - Microbiology 2024Quote: ... the RNA was diluted 1:5 in molecular grade water (Invitrogen). DNase-treated RNA (5 µL ...
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bioRxiv - Immunology 2024Quote: ... the RNA was diluted 1:5 in molecular grade water (Invitrogen). DNase-treated RNA (5 µL ...
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bioRxiv - Neuroscience 2024Quote: ... The RNA pellet was next dissolved in Nuclease Free Water (Invitrogen) and RNA quality assessed using a Nanodrop (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA pellets were resuspended in nuclease-free water (Thermo Fisher Scientific), and RNA concentration and purity were determined using a Nanodrop N1000 (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2024Quote: ... and 2 μl nuclease/RNase-free water (Thermo Fisher Scientific, USA) to a final volume of 10 μl ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA was resuspended in 8 µL nuclease-free water (Invitrogen, USA) and 120 nL dispensed using the I.DOT (Dispendix ...
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bioRxiv - Neuroscience 2024Quote: ... TALEN cRNAs were diluted in DNase/RNase-free water (Life Technologies) to the final concentration of 1 µg/μL and stored at −80°C before microinjection.
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Decoding protein glycosylation by an integrative mass spectrometry-based de novo sequencing strategybioRxiv - Biochemistry 2024Quote: ... LC-MS grade acetonitrile and water were purchased from Thermo Fisher Scientific (Nepean ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA was eluted in 50 uL of nuclease-free water (Ambion) and quantified via nanodrop ...
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bioRxiv - Cell Biology 2024Quote: ... Equal volumes of 1,6-Hexanediol and sterile distilled water (#15230162, Gibco) were mixed to prepare a 50%v/v stock solution (approximately 4000mM) ...
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bioRxiv - Bioengineering 2024Quote: ... and were eluted by 8 μL of nuclease-free water (Ambion). 1 μL of purified DNA was transformed into 25 μL of Endura electrocompetent cells (Lucigen ...
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bioRxiv - Bioengineering 2024Quote: ... Immediately after the addition of 6 μL DEPC water (Fisher Scientific), the column was centrifuged at 16,000 ×g for 2 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Waters) coupled to a Q Exactive Orbitrap mass spectrometer (Thermo Scientific) operating in negative ion mode ...
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bioRxiv - Cancer Biology 2024Quote: ... Resulting RNA was resuspended in Nuclease-free water (Fisher Scientific AM9937). 25ng RNA per sample was transcribed to cDNA using Superscript III First-Strand Synthesis System (Invitrogen 18080051) ...
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bioRxiv - Genetics 2024Quote: ... cDNA samples were diluted 2-fold with nuclease free water (Ambion) for their use in PCR reactions.
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bioRxiv - Developmental Biology 2024Quote: ... Purified DNA was diluted to 100 ng/l in water (Invitrogen™ UltraPure™ DNase/RNase-Free Distilled Water ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and dissolved in 20µl of DEPC-treated water (Ambion, Carlsbad, CA). To mitigate batch effects ...
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bioRxiv - Cell Biology 2020Quote: ... larger regions of Lysozyme-immunostained ileal sections (4-8 mm for each animal) were scanned with an EVOS system (Invitrogen) and the total number of Lysozyme-positive epithelial cells per linear ileal length was manually quantified using FIJI ...
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bioRxiv - Developmental Biology 2021Quote: The Emerald Green Fluorescent Protein (EmGFP) -encoding region from Vivid ColorsTM cDNA pcDNATM6.2/N-EmGFP-DEST Vector (Thermo Fisher Scientific) was placed upstream of the coding sequence of PmlVI (human PML transcript variant 5 ...
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bioRxiv - Developmental Biology 2021Quote: ... kcnk5b cDNA was isolated by RT-PCR from regenerating adult fin cDNA library and cloned into MCS region of pcDNA6-myc-6xHIS-tag plasmid (Invitrogen) or pBluescript harboring the hsp70 zebrafish promoter and GFP coding sequence surrounded by 2 miniTol2 sites ...
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bioRxiv - Evolutionary Biology 2021Quote: A 917 bp region containing the Dll319 CRE was cloned into the piggyGUE vector via the GATEWAY technology (Thermo Fisher). piggyGUE is the EGFP version of piggyGUM ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 5 kb of the 5’ flanking region of MpBLD10 with a SmaI site was introduced into the pENTR/D-TOPO vector (Invitrogen). The chimeric sequence was introduced into pMpGWB307 (Ishizaki et al ...
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bioRxiv - Developmental Biology 2020Quote: The full-length coding region of chicken EYA4 was PCR amplified from HH19-20 somite cDNA using SuperScript III Reverse Transcriptase kit (Invitrogen). Primer design used predicted EYA4 sequences for chicken (ENSGALG00000031656 ...
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bioRxiv - Immunology 2022Quote: Annealed oligonucleotides containing the sequence of the HCV 5’ppp poly-U/UC region (34) were in vitro transcribed using the MEGAshortscript T7 transcription kit (Ambion) followed by ethanol precipitation ...
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bioRxiv - Genomics 2022Quote: ... Bisulfite-PCR was conducted using primer sets (Integrated DNA Technologies; Table 1) designed against specific genomic regions with Methyl Primer Express v1.0 software (Thermofisher Scientific). PCR amplicons were cleaned with AmpureXP beads (#A63882 ...
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bioRxiv - Neuroscience 2021Quote: ... The resulting peptides and glycopeptides from each gel region were separately analyzed by LC-MS/MS using a Lumos Tribrid mass spectrometer (ThermoFisher). Proteins identified by peptides detected at <10 spectral matches (PSMs ...
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bioRxiv - Molecular Biology 2020Quote: The upstream region of the stx2 gene was analyzed by PCR in Veriti 96-Well Thermal Cycler (Applied Biosystems™). The proximity of the ninG gene with stx2 was evaluated using NinG/526 and 595 primers [15] ...
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bioRxiv - Biochemistry 2020Quote: ... Cryo-EM data in selected grid regions were collected in-house on a 200-keV Talos Arctica microscope (Thermo Fisher) with a post-column energy filter (Gatan ...
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bioRxiv - Plant Biology 2021Quote: ... the upstream region of SCL28 (2.2 kb) was amplified by PCR and cloned into the pENTR/D-TOPO vector (Thermo Fisher), then transferred to pBGGUS (Haga et al. ...
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bioRxiv - Plant Biology 2020Quote: ... the AGB1 genomic region from the start codon to immediately before the stop codon was inserted into pENTR-D-TOPO (Invitrogen) via TOPO cloning according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... gene sequence and 3’ untranslated region (300 bp directly downstream from stop codon) from Col-0 into the pDONR221 vector (Invitrogen) via a BP reaction and recombining it into the pFAST-R01 vector (Shimada et al. ...
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bioRxiv - Microbiology 2021Quote: Charged residues within the coiled-coil region were replaced with alanine by splice overlap PCR mutagenesis using Accuprime pfx polymerase (Invitrogen). PCR products containing the alanine mutation were cloned under the control of the ara promoter by insertion into pBAD33 by digestion with Kpn1 (NEB ...
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bioRxiv - Plant Biology 2021Quote: ... the coding region fragment was recombined into a Gateway binary vector pGWB405 (Tsuyoshi et al., 2009) using LR clonase (Invitrogen). Plasmid from a positive clone was transformed to Agrobacterium tumefaciens strain GV3101 ...
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bioRxiv - Plant Biology 2020Quote: Total RNA from laser captured embryo proper and suspensor regions was isolated using the PicoPure RNA Isolation Kit (Thermo Scientific) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... Standards for nifH reactions were obtained by amplifying one cloned nifH sequence with flanking regions of the plasmid vector (TOPO TA cloning Kit, Invitrogen). Standard curves were obtained by serial dilutions (E = 0.9 – 1.1 ...
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bioRxiv - Neuroscience 2022Quote: ... sheets of the olfactory epithelium from the dorsal roof region under the nasal bone were peeled and placed on a slide glass and sealed with live imaging solution (Invitrogen), and coverslipped ...
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bioRxiv - Cell Biology 2022Quote: ... Constructs of hCAP-H2 harboring charge-reversal mutations in the BC1 and BC2 regions (H2-BC1D, BC2D, and BC1/2D) were synthesized by ThermoFisher and subcloned into in pFastBac1 ...
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bioRxiv - Molecular Biology 2020Quote: ... Untranslated regions were cloned using synthetic DNA (Integrated DNA Technologies) or by isolation using 5′ RACE (RLM-RACE kit, Invitrogen). Template was PCR amplified using Phusion polymerase from the plasmids using the following primers ...
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bioRxiv - Plant Biology 2021Quote: ... coding regions of DGAT1s (AtDGAT1, RcDGAT1, GmDGAT1A, VgDGAT1A, HanDGAT1, JcDGAT1, and SiDGAT1) were cloned into the yeast vector pYES2 (Invitrogen). S ...
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bioRxiv - Neuroscience 2020Quote: ... β-neurexin-1 extracellular domain was cloned into the pCAGGS vector with a C-terminal human Fc tag and the ATD regions of GluA1–4 were cloned into a modified pDisplay vector (Invitrogen), as described previously (9) ...
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bioRxiv - Microbiology 2022Quote: ... targeting a region in the viral ORF1ab gene and using a QuantStudio™ 7 Real-Time PCR System (Applied Biosystems). Data were processed using GraphPad Prism software (V8.0 ...
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bioRxiv - Microbiology 2021Quote: ... A plasmid with plasmid pRR51 backbone and carrying a 4 kb region centered around gldL E49A and E59A codon substitutions was synthesized (Geneart, Thermofisher). The suicide plasmid pRR51-gldLE49A was then built by restoring the codon for E59 in the previously synthesized plasmid by site directed mutagenesis with oligonucleotide primers Fw-GldL-A59E and Rv-GldL-A59E ...
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bioRxiv - Genetics 2020Quote: ... 2-3ng of this DNA was used for each RT-qPCR reaction set up with primers specific to the centromeric dh repeat region using SYBR Master Mix (ThernoFisher) in QuantStudio3 (ThermoFisher). Fold enrichment was calculated using ΔΔCt method (27).
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bioRxiv - Genetics 2021Quote: ... The genomic region surrounding the CRISPR/Cas9 target site (741 bp) was PCR amplified using AccuPrime GC-Rich DNA Polymerase (Invitrogen) (Table S1) ...
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bioRxiv - Immunology 2020Quote: ... The preparations were co-stained for synaptic regions with 2 μg/mL AlexaFluor594-conjugated α-bungarotoxin (BTX) (B13423, Thermo Fisher) and imaged as described previously (Huijbers et al. ...
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bioRxiv - Microbiology 2021Quote: ... Standards for nifH reactions were obtained by amplifying one cloned nifH sequence with flanking regions of the plasmid vector (TOPO TA cloning Kit, Invitrogen). Standard curves were obtained by serial dilutions (E = 0.9 – 1.1 ...
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bioRxiv - Molecular Biology 2021Quote: ... Untranslated regions were cloned using synthetic DNA (Integrated DNA Technologies) or by isolation using 5′ RACE (RLM-RACE kit, Invitrogen). Template was PCR amplified using Phusion polymerase from the plasmids using the following primers ...
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bioRxiv - Microbiology 2020Quote: ... One-step RT-qPCR was performed with primers targeting the SARS-CoV-2 N region (BEI) using the EXPRESS One-Step Superscript qRT-PCR Kit (ThermoFisher) on an Applied Biosystems QuantStudio3 instrument ...
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bioRxiv - Molecular Biology 2022Quote: ... fused to a C-terminal trimeric GCN4 (tGNC4) zipper tag via a 5XGGS linker region was secreted from suspension HEK293 FreeStyle cells (ThermoFisher) in the presence of 20 μM kifunensine (Dextra ...