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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for 5R 3 4 5 6 Tetrahydro 5 phenyl N benzyloxycarbonyl 4 H 1 4 oxazin 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... One milligram of protein was incubated with 4 µg of antibody (IgG (Rb, Invitrogen) or HA (Rb ...
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bioRxiv - Biochemistry 2024Quote: The samples were run on one SDS-PAGE (NuPAGE 4-12% Bis-Tris, Invitrogen) in 1X MES buffer (Invitrogen) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... with 4 μg of a plasmid mixture (1:1:4 THR:RXR:TRE-Luc) in 200 μL Opti-MEM (Invitrogen, 11058-021) and added to the cells ...
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bioRxiv - Biophysics 2024Quote: ... for 2 hours at room temperature (RT) prior to overnight incubation at 4 °C with one of the following primary antibodies: Caspase 3 (Invitrogen, Cat# 700182), Insulin (SCBT ...
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bioRxiv - Biophysics 2020Quote: ... incubated with (N-γ-maleimidobutyryl-oxysuccinimide ester) crosslinker (4 mM in ethanol, Thermo Scientific), rinsed with ethanol and dried thoroughly in a sterile biosafety cabinet ...
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bioRxiv - Genomics 2020Quote: ... at 4°C and washed twice with ice cold HBSS (ThermoFisher, Cat. N: 14025092). After the final wash cells were resuspended in PBS with 1% BSA (ThermoFisher ...
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bioRxiv - Neuroscience 2020Quote: ... The culture medium was refreshed every day and cells were passaged every 4-5 days using 1 mg/mL Dispase II (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... The membrane was blocked with 5% (w/v) skimmed milk overnight at 4°C and probed with anti-DsbA (1:1000, Thermo Fisher Scientific) or anti-AP (1:5000 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were collected by spinning at 1000g for 5 min at 4°C and cell pellets were resuspended in 1 mL of Trizol (Thermo Fisher Scientific). Total RNA was extracted according to manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... Cells were centrifuged at 300 x g for 5 min at 4°C and treated with 1 mL of Ammonium-Chloride-Potassium (ACK) Lysing Buffer (Gibco; cat#A1049201). After 2 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... The culture medium was refreshed every day and cells were passaged every 4–5 days using 1 mg/mL Dispase II (Thermo Fisher Scientific). Cells were routinely tested for mycoplasma contamination ...
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bioRxiv - Cancer Biology 2024Quote: ... De-crosslinking was conducted using 4 µl of 5 M NaCl and 1 µl of 20 mg/ml proteinase K (Thermo Fisher Scientific) for 4 h at 60 °C ...
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bioRxiv - Genomics 2024Quote: ... The membrane was blocked with 5% non-fat milk for 1 hour at RT and incubated overnight at 4°C with primary antibody [Sfrp1 (Invitrogen, MA5-38193), Nr3c1 (Cell signaling ...
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bioRxiv - Neuroscience 2024Quote: ... The culture medium was refreshed every day and cells were passaged every 4–5 days using 1 mg/mL Dispase II (Thermo Fisher Scientific). Cells were routinely tested for mycoplasma contamination (17 ...
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bioRxiv - Synthetic Biology 2024Quote: ... Membranes were incubated overnight (50 rpm, 4°C) with either mouse anti-His monoclonal (Thermo Fisher; 1:3000 in 5% skimmed milk) or anti-IFNa2 primary antibody (abcam ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were immunoblotted at 4°C overnight with the following antibodies in 5% BSA/1x TBST: MKP1/DUSP1 (1:1000, Invitrogen #MA5-32480), DUSP3 (1:2000 ...
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bioRxiv - Genetics 2023Quote: ... Total RNA was extracted from n = 3–4 biological replicates per sample using the PureLink RNA Mini Kit (Invitrogen, Waltham, MA, USA), and sample quality was evaluated using a TapeStation ...
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bioRxiv - Molecular Biology 2021Quote: ... 10’000 rcf for 30 min and then ultracentrifuged at 120’000 rcf at 4 °C for 2 h (TH 64.1 rotor, Thermo Fisher Scientific). The epididymosomal pellet was then washed in PBS at 4 °C and ultracentrifuged at 120’000 rcf at 4 °C for 2 h ...
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysates were incubated for 2 h at 4°C with IgG-coupled Dynabeads (Dynabeads M-270 Epoxy; Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... MBL) and an additional 2 h at 4°C with 20 μL of a slurry of IgG-conjugated Dynabeads (Invitrogen). After washing the beads five times with IP buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Antibody–protein complexes were recovered during 2 h incubation at 4°C with protein A/G magnetic beads (Thermo Scientific), followed by duplicate washes of 15 min with low-salt wash buffer (0.1% SDS ...
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bioRxiv - Immunology 2024Quote: ... filtered through a 0.45 µm pore-sized filter to remove cell debris and concentrated by centrifugation at 100000 g for 2 h at 4°C (Thermofisher WX Ultra80 centrifuge ...
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bioRxiv - Biochemistry 2024Quote: ... that was pre-conjugated to Protein A/G beads (Pierce, 88802) for 2 h 4 °C (note: pre-conjugation crosslinked using 20 mM dimethyl pimelimidate (DMP, ThermoFisher) in borate buffer (40 mM boric acid ...
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bioRxiv - Biochemistry 2024Quote: ... that was pre-conjugated to Protein A/G beads (Pierce, 88802) for 2 h 4 °C (note: pre-conjugation crosslinked using 20 mM dimethyl pimelimidate (DMP, ThermoFisher) in borate buffer (40 mM boric acid ...
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bioRxiv - Immunology 2022Quote: SMGs were fixed for 6-8 hours in 4% paraformaldehyde (PFA; Thermo Scientific) at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... and passaged every 4-6 days with 0.5mM ethylenediaminetetraacetic acid (EDTA, Invitrogen 15575020) in Dulbecco’s Phosphate-Buffered Saline (DPBS ...
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bioRxiv - Cell Biology 2023Quote: ... 6% Tris-glycine or NuPAGE 4-12% Bis-Tris gels (Invitrogen; cat# NP0321BOX) and transferred to Immobilon-P PVDF membranes (Millipore Sigma ...
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bioRxiv - Microbiology 2023Quote: ... 4 and 6 hpi RNA was extracted from cells using TRIzol (Invitrogen; 15596026). RNA was isolated and precipitated following the manufacturer’s institutions and 200ng was reverse transcribed into cDNA using the ABI cDNA synthesis kit (Applied Biosystems ...
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bioRxiv - Bioengineering 2024Quote: ... Spherical beads (4, 6, 8 and 10 μm diameter) were sourced from Invitrogen.
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bioRxiv - Developmental Biology 2022Quote: ... and pGL4.74-Renilla luciferase plasmids in a ratio of 1:1:0.1 (4 μg/6-well dishes) by using Lipofectamine LTX PLUSTM (Invitrogen). The luciferase activity was assayed using the Dual-Luciferase Reporter Assay Kit (Promega) ...
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bioRxiv - Microbiology 2021Quote: ... lysates were spun at 10,000 x g for 10 minutes at 4 °C prior to reaction with 4- acetamido-4’-maleimidyl-stilbene-2,2’-disulfonic acid (AMS) (ThermoFisher Scientific). AMS alkylation was performed by vortexing the lysates in 15 mM AMS ...
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bioRxiv - Cell Biology 2020Quote: ... Cultured colonies from organoids were fixed with 4% PFA for 2-4 hrs at room temperature followed by immobilization with Histogel (Thermo Scientific) for paraffin embedding ...
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bioRxiv - Biophysics 2023Quote: ... Samples were mildly fixed with 4% PFA for 4 min before immunostaining using anti SARS-CoV-2 Spike Protein S1 (Cat# PA5-114447, ThermoFisher Scientific) and anti-human IgG (Fc)-Alexa 488 (Cat# 109-545-170 ...
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bioRxiv - Cancer Biology 2023Quote: ... with a Dionex IonPac AS11-HC-4 μm column (2 mm i.d. × 250 mm, 4 μm particle size, Thermo Fisher Scientific) coupled with a Q Exactive ...
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bioRxiv - Systems Biology 2023Quote: ... with a Dionex IonPac AS11-HC-4 μm column (2 μm i.d. × 250 mm, 4 μm particle size, Thermo Fisher Scientific) coupled with a Q Exactive high-performance benchtop quadrupole Orbitrap mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... sham and uPA-CBP were anesthetized (4% isoflurane, 2 liters of oxygen) and transcardial perfusion fixed with 4% paraformaldehyde (PFA) (Cat #50-980-494, Fisher Scientific) in PBS 1x until stiff ...
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bioRxiv - Developmental Biology 2024Quote: ... with a Dionex IonPac AG11-HC-4 μm guard column (2 mm i.d × 50 mm, 4 μm particle size, Thermo Fisher Scientific) and a Dionex IonPac AS11-HC-4 μm column (2 mm i.d × 250 mm ...
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bioRxiv - Developmental Biology 2024Quote: ... and a Dionex IonPac AS11-HC-4 μm column (2 mm i.d × 250 mm, 4 μm particle size, Thermo Fisher Scientific) coupled to a Q Exactive mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... TFA and 1 μL of this dilution and 1μL of the undiluted peptides from bands 3 and 4 were injected onto the trapping column (Thermo Scientific, PepMap100, C18, 300 μm × 5 mm), using a partial loop injection ...
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bioRxiv - Synthetic Biology 2024Quote: ... cultures were plated on LB agar plates containing 60 μg/mL 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside (X-gal; Thermo Fisher Scientific catalogue no. R0402). Antibiotics in media for bacterial growth were used at the following working concentrations ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... Infected cell pellets were centrifuged at 5000xg for 5 min at 4 °C and resuspended in Trizol (Ambion).
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bioRxiv - Cancer Biology 2021Quote: ... Samples were then stained with 5 µL FITC anti-human DR4 (Thermo Fisher Scientific, Clone DR-4-02) for 30 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... infected Jurkat cells were initially preloaded with 5 µM of CellTracker CMAC (7-amino-4-chloromethylcoumarin) (Life Technologies) and cocultured for 6 or 24 h with MDMs plated onto coverslips ...
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bioRxiv - Neuroscience 2021Quote: ... the washing medium was removed and 80 L of 5 M cell permeant Fluo-4 AM (Life technologies) in neurobasal medium was added to wells at room temperature and incubated at 37 °C for 30 minutes ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 4 mM DTT and 20% glycerol) at 30 °C in a Cytation 5 imaging reader (Thermo Fisher Scientific) with filters for excitation at 360/40 nm and emission at 460/40 nm ...
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bioRxiv - Bioengineering 2020Quote: ... WT-PGP1 were labeled with 5 μM CellTracker™ Blue 7-amino-4-chloromethylcoumarin CMAC (Molecular Probes, #C2110), iNeuron-PGP1 was labeled with 2.5 μM CellTracker™ Green CMFDA (Molecular Probes ...
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bioRxiv - Cell Biology 2021Quote: ... 5-10µg of protein per sample was run on a 4%–12% NuPAGE Bis-Tris Gel (ThermoFisher Scientific) and then transferred to PVDF membrane by liquid transfer using NuPAGE Transfer buffer (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... The cells were centrifuged at 400 g for 5 min at 4°C and resuspended in DPBS (GIBCO) supplemented with 5% FBS ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were boiled for 5 minutes then electrophoresed in pre-cast 4-12% NuPAGE Bis-Tris Gels (Invitrogen). Following transfer ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were further lysed via sonication (10 sec on 5 sec off, repeat 3x, ThermoFisher: FB120, 4°C) and protein was clarified by centrifugation at 21,000 x g for 5 min at 4°C ...