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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for 5R 3 4 5 6 Tetrahydro 5 phenyl N benzyloxycarbonyl 4 H 1 4 oxazin 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... SDS-PAGE gels were loaded with 5-15 μL of lysate per lane (NuPAGE 4-12% Bis-Tris; Invitrogen). Lysates were diluted with additional 1x NuPAGE LDS buffer if necessary ...
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bioRxiv - Biochemistry 2023Quote: ... heated for 5 minutes at 95°C and loaded on 4-12% Bis-Tris NuPage gels (Thermo Fisher Scientific). After electrophoretic separation ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were clarified by centrifugation (18,253 xg, 5 min, 4°C) and protein concentrations determined by absorbance on a NanoDrop instrument (ThermoFisher). Equal amounts of lysate were mixed with 4x loading dye (2% SDS ...
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bioRxiv - Developmental Biology 2023Quote: ... transgenic hg13 larvae (mmachchg13/hg13 Tg(col2a1a:EGFP)) were staged and fixed at 5 DPF with 4% paraformaldehyde (Fisher Scientific). Larvae were dissected for genotyping and unbiased imaging was performed on the remaining head tissue ...
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bioRxiv - Developmental Biology 2023Quote: ... Larvae were maintained in embryo media at 28.5°C until 5 DPF and fixed in 4% paraformaldehyde (Fisher Scientific) at room temperature for 1 hour and stored in 1x PBS (Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were subsequently centrifuged for 5 minutes at 200 x g and resuspended in 4% paraformaldehyde (Thermo Scientific Chemicals) for 30 minutes at 22°C ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were rinsed with PBS (4 x 5 min) and mounted on microscope slides with ProLong Gold (ThermoFisher Scientific). Slices were kept in the fridge for at least two weeks before confocal imaging ...
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bioRxiv - Physiology 2024Quote: Platelet intracellular Ca2+ was measured as described previously using 5 µM Fluo-4 fluorescence dye (Invitrogen, Waltham, Massachusetts, USA).20 1 x 105 washed platelets were labeled with Fluo-4 for 30 min at room temperature (RT) ...
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bioRxiv - Cancer Biology 2024Quote: Passaging of ROBO1+ sorted Rounded/CBC cultures every 4-5 days is best done by incubating cells with 0.25% trypsin-EDTA (Gibco) for 1-2 min at RT/37°C ...
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bioRxiv - Molecular Biology 2024Quote: 786-O cells (5 × 104) were seeded in 4-well Nunc Lab-Tek II chamber slides (Thermo Fisher Scientific) and cultured for 2 days ...
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bioRxiv - Molecular Biology 2024Quote: ... 786-O cells (5 × 104) were seeded in 4-well Nunc Lab-Tek II chamber slides (Thermo Fisher Scientific) and cultured for 2 days ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained in FACS buffer (PBS supplemented with 2% FBS and 5 mM EDTA) for 30 min at 4°C using the following antibodies: eFluor450 anti-mouse CD4 (GK1.5; Invitrogen), BV605 anti-mouse CD8 (53-6.7 ...
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bioRxiv - Cell Biology 2024Quote: ... and the supernatant was replaced with 5 mM glucose-containing RPMI 1640 with Di-4-ANEPPDHQ (Thermo Fisher Scientific) at 2 µg/mL ...
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bioRxiv - Immunology 2024Quote: ... heated to 70°C for 5 min and ran on a 4-12% Nupage precast gel (Thermo Fisher Scientific). Protein were then detected using InstantBlue™ Coomassie Stain (Abcam ...
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bioRxiv - Biophysics 2024Quote: ... 5 μl of protein lysate was separated by gel electrophoresis on a NuPAGE 4-12% Bis-Tris Gel (ThermoFisher) and transferred by semi-dry transfer in NuPAGE transfer buffer (ThermoFisher ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... centrifuged at 500xg for 5 minutes at 4°C using a swinging bucket rotor (Sorvall Legend RT, Thermo Fisher), and then pellets were washed with an additional 1 mL cold lysis buffer and incubated on ice for an additional 5 minutes.
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bioRxiv - Cancer Biology 2024Quote: ... then denatured at 95°C for 5 minutes and loaded on 4-12% NuPAGE™ Bis-Tris gels (Invitrogen). Samples were transferred to a nitrocellulose membrane using a semi-wet transfer ...
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bioRxiv - Cell Biology 2024Quote: ... was boiled for 5 minutes at 95°C and separated on a 4-12% Bis-Tris gel (NP0321BOX, Invitrogen) in MES running buffer (NP0002 ...
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bioRxiv - Immunology 2024Quote: ... 5×10-5 M 2-mercaptoethanol (Gibco) and 50µg/mL Gentamicin (Lonza ...
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bioRxiv - Neuroscience 2023Quote: ... for 2 minutes followed by ice cold 4% PFA for 4 min with a flow rate of 6ml/min and post-fixed (overnight at 4°C) in 4% PFA (Thermo Fisher Scientific) in 1x PBS (pH 7.4) ...
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bioRxiv - Immunology 2024Quote: ... and pMD2.G at a ratio of 4:3:1 using Lipofectamine 3000 kit (Thermo Fisher Scientific). 2D3 Jurkat 76.7 cells expressing human CD8+ and NFAT-GFP (Morimoto et al ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were resolved on 4-12% or 6% NuPAGE Bis-Tris gels (ThermoFisher Scientific) and transferred onto nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... 6 × 105 of isolated MLEC were cultured in a 4-well chamber slide (Nunc Lab-Tek Chamber Slide System ...
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bioRxiv - Cell Biology 2020Quote: ... whole 6-day-old Col_0 seedlings were incubated with 4 μM FM4-64 (Invitrogen) solution in 1/2 strength MS liquid medium between 2 coverslips at 25 °C or 12 °C for 30 minutes.
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bioRxiv - Cancer Biology 2020Quote: ... 4 and 6 days after plating with alamarBlue Cell Viability Reagent (Thermo Fisher Scientific) and fluorescence at 585 nm was measured on a Spectra Max3 plate reader (Molecular Device ...
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bioRxiv - Cell Biology 2020Quote: ... The washed beads were boiled for 6 min in 4× NuPAGE loading buffer (Invitrogen) supplemented with 1.4 M β-mercaptoethanol and analyzed by Western blot.
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bioRxiv - Cell Biology 2023Quote: ... Samples were resolved on 4-12% or 6% NuPAGE Bis-Tris gels (ThermoFisher Scientific) and transferred onto nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Developmental Biology 2023Quote: ... PXGL cells were passaged every 4-6 days using TrypLE (12604013, Thermo Fisher Scientific) for 3 min ...
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bioRxiv - Microbiology 2021Quote: ... Cell monolayers were then stained with 3 mL of overlay containing a 1:1 mixture of 1.2% oxoid agar with 4% neutral red (Gibco) and 2X DMEM with 2% (vol/vol ...
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bioRxiv - Systems Biology 2023Quote: ... 1 vein graft slide containing 2-4 cross sections was double stained with the general macrophage marker Mac-3 (rat anti-mouse, Fisher Scientific, B550292, 1:600) and either iNOS (rabbit anti-mouse ...
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bioRxiv - Microbiology 2020Quote: ... as well as the fluorescent probe N-phenyl-1-naphthylamine (NPN; Acros Organics, USA) at a final concentration of 10 μM ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Lot 134874), and 4-(2-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, purity ≥ 99.%, CAT #BP310-1, Lot 052975) were from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Microbiology 2020Quote: ... 100μm lung slices were washed twice in 200 mM HEPES buffer then incubated for 20 min in a staining solution containing: 0.715 μM DAPI (4′,6-diamidino-2-phenylindole) (ThermoFisher Scientific D1306), 10 mg/L BODIPY 493/503 (4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene ...
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bioRxiv - Bioengineering 2021Quote: ... and the cells were incubated for 50 minutes and DAPI (4′,6′-diamino-2-phenylindole) (Molecular Probes, Thermo Fisher Scientific, USA) for 5 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... and the cells were incubated for 50 minutes and DAPI (4′,6′-diamino-2-phenylindole) (Molecular Probes, Thermo Fisher Scientific, USA) for 5 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... slides were washed 3 x 5 min with PBS-TX and once with 0.1 M phosphate buffer pH 7.4 (0.08 M sodium phosphate dibasic and 0.02 M sodium phosphate monobasic) prior to coverslipping with ProLong Gold antifade reagent with 4’,6-diamidino-2-phenylindole [DAPI] (Invitrogen, P36931).
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bioRxiv - Neuroscience 2021Quote: Hair cell nuclei were specifically labeled by briefly exposing free-swimming larvae to 4’,6-Diamidino-2-Phenylindole (DAPI; Invitrogen/ThermoFisher) diluted to 2.5 μg/ml in E3 ...
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bioRxiv - Genetics 2021Quote: ... cells were washed and suspended in flow cytometry buffer containing 1µg/ml 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2020Quote: ... once with 1X SSC at room temperature for 15 min and twice with 0.5X SSC at room temperature for 15 min and finally with 1X PBS buffer containing 0.5 µg/mL 4′,6-diamidino-2-phenylindole (DAPI) and mounted onto ProLong Gold antifade reagent (Thermo Fisher Scientific) mounting media.
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bioRxiv - Microbiology 2022Quote: ... diluted 1:200 in blocking buffer for 30 min and mounted with ProLong Gold Antifade with DAPI (4′,6-diamidino-2- phenylindole; Thermo Fisher). Confocal laser micrographs were obtained with Zeiss LSM 880 laser microscope and analyzed with Zen black and Fiji software ...
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bioRxiv - Biophysics 2022Quote: ... gels were functionalized with 0.5 mg/ml solution of sulfosuccinimidyl 6-(4′-azido-2′-nitrophenylamino) hexanoate (Sulfo-SANPAH) (Thermo Fisher Scientific) prepared in 50 mM HEPES buffer (Santa Cruz Biotechnologies) ...
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bioRxiv - Microbiology 2020Quote: ... cells were extensively washed and incubated with secondary antibodies in the presence of 4′,6-diamidino-2-phenylindole (DAPI, Molecular Probes) for 45 min at RT ...
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bioRxiv - Microbiology 2022Quote: ... After extensive washing slides were air-dried and mounted in Gold Antifade containing 4’,6-diamidino-2-phenylindole (DAPI) (P3693; Life Technologies) for imaging ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed in PBS-T and mounted under Prolong gold fluorescence media with 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen, P36931).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Alexa Fluor 488–conjugated phalloidin (#A12379) and 4’,6-diamidino-2-phenylindole (DAPI) (#1306) were obtained from Invitrogen (Carlsbad, CA, USA). IRDye800CW-labeled anti-rabbit secondary antibody (#926-32211 ...
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bioRxiv - Neuroscience 2020Quote: ... slides were washed and stained with 4′,6-Diamidin-2-phenylindol (DAPI, 5μg/ml) before mounting coverslips with ProlongTM Gold Antifade reagent (Thermo Fisher Scientific). Images were acquired using a LSM 710 confocal microscope (Zeiss ...
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bioRxiv - Immunology 2021Quote: ... The isolated nuclei were centrifuged at 500 x g for 5 min at 4°C and respectively resuspended with Nuclei wash buffer stained with 10 µg/ml 4′,6-diamidino-2-phenylindole (DAPI) before FACS (5mg/ml, Invitrogen D1306). After sorting using purity mode ...
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bioRxiv - Biochemistry 2020Quote: ... Nuclei were stained with 10.9 μΜ 4’,6-diamidino-2-phenylindole (DAPI) and cover glasses were mounted in ProlongGold (Life Technologies). Cells were incubated 3x for 5 min in blocking solution to remove unbound antibodies ...
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bioRxiv - Immunology 2023Quote: ... Nuclei were detected and cover slips were attached using 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) containing Prolong Diamond Anti-Fade (Life Technologies). Slides were imaged on a Keyence BX-X810 microscope.
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bioRxiv - Cell Biology 2023Quote: ... A final wash with PBS was performed before mounting the cells in ProLong® Gold Antifade Mounting medium with DAPI (4’,6-diamidine-2-phenylindole)(Invitrogen).