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Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for Inhibin beta C chain INHBC Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Real-time polymerase chain reactions were performed with TaqMan Universal PCR MasterMix (Applied Biosystems) or SYBR Green MasterMix (Fermentas ...
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bioRxiv - Immunology 2021Quote: ... Heavy and light chain plasmids were co-transfected into Expi293F cells (Thermo Fisher Scientific) for recombinant mAb production ...
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bioRxiv - Microbiology 2019Quote: ... Polymerase chain reaction was conducted using the Advantage 2 PCR system (Invitrogen CAT# 639137) using primers targeting the conserved RNA-dependent RNA polymerase gene of S ...
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bioRxiv - Molecular Biology 2021Quote: ... Polymerase chain reactions (PCR) were carried out with Phusion High-Fidelity DNA Polymerase (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative polymerase chain reaction (qPCR) was performed using Power SYBR Green kit (Invitrogen; 4368577) with signals detected by ViiA7 System (Life Technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative polymerase chain reaction (qPCR) was performed using Power SYBR Green kit (Invitrogen; 4368577) with signals detected by ViiA7 System (Life Technologies) ...
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bioRxiv - Bioengineering 2021Quote: ... A rat-specific primary antibody for skeletal muscle myosin heavy chain (MA1-35718, ThermoFisher) was diluted 1:5 in TBS-NGS ...
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bioRxiv - Cell Biology 2022Quote: ... The primary antibodies used were anti-dynein heavy chain (1:250, Thermo Fisher Scientific) and anti-vinculin (1:1000 ...
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bioRxiv - Cell Biology 2019Quote: ... The quantitative polymerase chain reaction (qPCR) was performed using VeriQuest Mastermix (Thermo Fisher Scientific) for SYBR Green and Taqman primer sets respectively ...
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bioRxiv - Synthetic Biology 2020Quote: ... by diluting heavy and light chain construct DNA in OptiMem serum-free media (Gibco) before the addition of and incubation with FectoPro (Polyplus Transfection ...
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bioRxiv - Physiology 2023Quote: ... Quantitative polymerase chain reaction (qPCR) was performed using Power Sybr Green (Applied Biosystems, 4367659) and measured using an ABI QuantStudio 3 sequence detection system (Applied Biosystems) ...
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bioRxiv - Neuroscience 2022Quote: ... Real-time polymerase chain reaction (PCR) was performed using TaqMan assays (Thermo Fisher Scientific) on a QuantStudio™ 5 real-time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: Quantitative polymerase chain reaction (qPCR) was performed using a QuantStudio 3 (Thermo Fisher Scientific) instrument ...
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bioRxiv - Immunology 2023Quote: ... Transfection with heavy and light chain plasmids was performed using the Expifectamine293 kit (Gibco) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... a PCR (Polymerase Chain Reaction) master mix (TaqMan Fast Advanced Master Mix, Applied Biosystems) and the following validated primers (TaqMan ...
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bioRxiv - Biophysics 2023Quote: ... Reverse polymerase chain reaction (PCR) was performed to generate cDNA using SuperScript III (Invitrogen). For quantitative reverse transcriptase-PCR ...
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bioRxiv - Molecular Biology 2023Quote: ... All polymerase chain reaction (PCR) reactions were performed using Phusion polymerase (Thermo Fisher F530S) or Phusion Flash High-Fidelity PCR Master Mix (Thermo Fisher F548L ...
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bioRxiv - Cell Biology 2023Quote: ... Real time polymerase chain reaction was performed on ViiA™ 7 system (Applied Biosystems). Total volume of qPCR reaction was 20µl and it contained 10µl of 2x qPCR Master Mix Low ROX (Eurogentec) ...
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bioRxiv - Immunology 2024Quote: ... equal mass of heavy and light chain plasmids were transfected into Expi293F cells (Gibco) per manufacturer’s instructions and harvested 6 days after transfection ...
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bioRxiv - Biophysics 2021Quote: A stable β2AR-overexpressing cell line was produced by transfecting CHO-K1 cells (ECACC) with a plasmid coding for the human β2AR with C-terminal GFP using lipofectamine 2000 (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and the ELISPOT plate was incubated for 4 hours at 4°C with biotinylated goat anti-human IgG Fc (Invitrogen), followed by incubation for 1 hour at room temperature with horseradish peroxidase (HRP)–conjugated avidin (Vector Laboratories) ...
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bioRxiv - Molecular Biology 2019Quote: Huh-7 (human hepatoma) cells were maintained at 37 °C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies) supplemented with 10% (vol/vol ...
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bioRxiv - Microbiology 2020Quote: ... and human hepatoma Huh7.5 cells83 were maintained at 37°C and 5% CO2 in Dulbecco’s Modified Eagle Medium (Invitrogen, Paisley, UK) supplemented with 10% heat inactivated fetal bovine serum (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: Mammalian expression plasmids encoding SARS VHH72 and MERS VHH-55 (73) with a C-terminal a monomeric human Fc tag were transfected into ExpiCHO cells (Thermofisher) using ExpiFectamine as manufacterer’s recommendation ...
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bioRxiv - Cell Biology 2020Quote: Parent CHO-K1 and CHO-K1 cells stably expressing extracellular ACP-tagged human insulin receptors were maintained at 37°C with 5% CO2 in HAM’s F-12 medium (Thermo Fisher) supplemented 10% fetal bovine serum (FBS) ...
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bioRxiv - Neuroscience 2020Quote: ... β-neurexin-1 extracellular domain was cloned into the pCAGGS vector with a C-terminal human Fc tag and the ATD regions of GluA1–4 were cloned into a modified pDisplay vector (Invitrogen), as described previously (9) ...
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bioRxiv - Molecular Biology 2019Quote: ... were incubated for 15 minutes at 37°C in the presence of Alexa-fluor 555 labelled human transferrin (Thermo Fisher) (25 μg/ml ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... as well as human hepatoma Huh7.5 cells28 were maintained at 37 °C with 5% CO2 in Dulbecco’s Modified Medium (DMEM) (Invitrogen, Paisley, UK) containing 10% heat inactivated fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... SK-N-FI) and NCI-H23 cells (human NSCLC) were cultivated at 37°C with 5 % CO2 in RPMI 1640 medium (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... cells were transfected with an expression plasmid encoding human ACE2 - C-terminally fused to the GFP - using Lipofectamine 2000 (Invitrogen). Two days post transfection ...
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bioRxiv - Cell Biology 2021Quote: ... falciparum cell lines were cultured in vitro in human erythrocytes (UK Blood and Transfusion Service) at 37°C in RPMI-1640 (Gibco) based media supplemented with 5% (w/v ...
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The MuSK-BMP pathway maintains myofiber size in slow muscle through regulation of Akt-mTOR signalingbioRxiv - Cell Biology 2022Quote: ... Serum was diluted 1:1000 in 1/10 block overnight at 4°C and detected using goat anti-human IgG Alexa Fluor 555 (Invitrogen) diluted 1:1000 and counterstained with AlexaFluor488-conjugated α-bungarotoxin (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: SF268 (human glioblastoma) cells were cultured at 37 °C with 5% CO2 in RPMI medium supplemented with 10% FBS (Gibco). Vero (African green monkey kidney ...
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bioRxiv - Immunology 2023Quote: ... and incubated at 37°C in media with or without 0.5 ng/ml (1.0 unit/ml) human IFN-γ (ThermoFisher Scientific) at time points ranging from 5 minutes to 24 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... human kidney) were maintained at 37°C and 9% CO2 in a 75% humidified atmosphere in Expi293 Expression Medium (GIBCO). The Plasmid was transiently transfected using Expifectamine 293 (GIBCO ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary human peripheral mononuclear blood cells (PBMCs) were cultured at 37 °C in 5% CO2 in RPMI 1640 (ThermoFisher, 61870143) supplemented with 10% FBS ...
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bioRxiv - Physiology 2023Quote: ... followed by C-terminal region of human FGF-21 (amino acids 166-209) into a vector pCEP4 (Thermo Fisher Scientific). The construct was then transiently transfected to Expi293F cells (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Primary antibody incubation was carried out overnight at 4 °C with human-CD68 monoclonal antibody (cat. No. 14-0688-82, Invitrogen) and CD19 Rabbit polyclonal antibody (cat ...
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bioRxiv - Immunology 2019Quote: ... Samples were prepared using the Ion PGM™ Hi-Q™ View OT2 and Ion PGM™ Hi-Q™ View Sequencing kits (ThermoFisher Scientific) and 4 barcoded libraries were combined per Ion 316™ Chip kit (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... a 6x stock solution of the beta-lactamase substrate CCF2-AM (Thermo Fisher, K1032) was added to the media over 10 T1/2 cells so that the final concentration of CCF2-AM was 1x ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated in beta actin primary antibody (1:1000, Thermo Fisher Scientific, Cat. #MA515739) for 1 hour at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... We manipulate concentration of transforming growth factor beta-1 (TGF-β, Invitrogen, CA, USA) between source and sink channels to develop chemical gradients in the center channel ...
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bioRxiv - Bioengineering 2022Quote: ... mouse monoclonal beta-catenin Alexa Fluor 647-conjugated antibody (Thermo Fisher Scientific; 1:100); mouse monoclonal VE-cadherin Alexa Fluor 488-conjugated antibody (Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... Each reaction was normalised to a beta-2-microglobulin (B2M) control (Hs00984230_m1; Applied Biosystems). For quantifications using SYBR-green ...
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bioRxiv - Synthetic Biology 2020Quote: ... 3.3 nM of 5-chloromethylfluoresecein di-beta-D-galactopyranoside (CMFDG) (Invitrogen, Waltham, MA, USA) was added to the reaction solution ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies include Anti-beta tubulin (1:500, mouse monoclonal, 2 28 33, Invitrogen), Anti-Hec1 (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... and beta Actin mouse Monoclonal Antibody (AC-15) (# Cat. No. MA1-91399, Thermo Scientific). The secondary antibodies used were Anti-rabbit IgG (H+L ...
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bioRxiv - Genetics 2023Quote: ... 0.1 mM beta-mercaptoethanol,100 U/ml penicillin and 100 mg/ml streptomycin (Gibco)) on SNL-HBP feeder cells ...
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bioRxiv - Biophysics 2023Quote: Primary antibodies used were: Mouse-beta tubulin monoclonal antibody (Thermo Fisher Scientific, 32–2600), Rabbit-anti-Tom20 polyclonal antibody (Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated overnight with primary antibody directed against Aβ (Invitrogen, beta Amyloid (1-40) Polyclonal Antibody ...