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Citations for Thermo Fisher :
1251 - 1300 of 10000+ citations for lefty A TGF beta 4 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: Immunoprecipitation from HEK293 cell lysates was conducted using Dynabeads protein G magnetic beads (Invitrogen, Thermo Fisher Scientific) according to manufacturer’s protocol with a following modification ...
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bioRxiv - Biochemistry 2020Quote: Immunoprecipitation from HEK293 cell lysates was conducted using Dynabeads protein G magnetic beads (Invitrogen, Thermo Fisher Scientific) according to manufacturer’s protocol with a following modification ...
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bioRxiv - Bioengineering 2022Quote: ... hACE-2-expressing HEK293 cells were seeded on a Nunc Lab-Tek Chamber Slide system (Thermo Fisher) and cultured overnight ...
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bioRxiv - Biochemistry 2022Quote: ... HEK293-PSMB2-HB cells were transfected with 5 μg of the cloned plasmid using Lipofectamine 3000 (Invitrogen). After 48 h incubation ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 Jump-In and Flp-In TREx cells that allow doxycycline-dependent transgene expression were from Invitrogen. Cell lines were checked for mycoplasma infection by PCR or ELISA ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293-based EGFP reporter and Hela cells were maintained in Dulbecco’s Modified Eagle’s medium (DMEM, ThermoFisher Scientific) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: ... growth media containing DMEM (HEK293) or DMEM/F12 (CHO) (both high glucose, pyruvate, Thermo Fisher Scientific (TFS)) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... HEK293S GnTI- cells were grown at 37°C with 8% carbon dioxide in Freestyle 293 medium (Gibco) supplemented with 2% (v/v ...
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bioRxiv - Neuroscience 2020Quote: ... Transfected HEK293 cells were solubilized in 0.1-1% (w/v) n-dodecyl-β-D-maltoside (ThermoFisher Scientific), 1 mM phenylmethylsulfonyl fluoride (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... and 5% CO2 in DMEM (for HEK293 and HeLa) supplemented with 10% Fetal bovine serum (Invitrogen, Switzerland) and 1% of Penicillin/Streptomycin (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: HEK293 cells grown to 30-40% confluence in 6-well dishes were transfected with Lipofectamine 2000 (Invitrogen) in 35mm plates with 0.5 μg of pLKO shRNA or pLVX expression plasmids (Takara) ...
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bioRxiv - Cell Biology 2020Quote: Hek293 cells (ATTC® CRL-1573) were grown in DMEM + GlutaMAX™-I (Gibco, Invitrogen, Paisley, UK) with 10% fetal bovine serum (Cornig #35-010 ...
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bioRxiv - Cell Biology 2020Quote: Hek293 cells (ATTC® CRL-1573) were grown in DMEM + GlutaMAX™-I (Gibco, Invitrogen, Paisley, UK) with 10% fetal bovine serum (Cornig #35-010 ...
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bioRxiv - Synthetic Biology 2019Quote: Luc14 and Gal4-EED/luc HEK293 cells were grown in Gibco DMEM high glucose 1× (Life Technologies) with 10% Tet-free Fetal Bovine Serum (FBS ...
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bioRxiv - Neuroscience 2019Quote: ... The inter-molecular tau FRET biosensor was generated by transiently transfecting HEK293 cells using Lipofectamine 3000 (Invitrogen) with tau-GFP and tau-RFP (1:20 DNA plasmid concentration ratio) ...
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bioRxiv - Molecular Biology 2021Quote: ... were transfected into HEK293 T-Rex cells with Lipofectamine CRISPRMAX Cas9 Transfection Reagent (Thermo Fisher Scientific, CMAX00008). After 48 h of incubation ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 stable cells overexpressing Flag-INTS118 and Flag-Ago215 were cultured in DMEM media (Gibco, #11965-084) containing puromycin and supplemented with 10% FBS (Atlas Biologicals ...
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bioRxiv - Cancer Biology 2019Quote: ... Both HEK293 and HEK293T cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco/Invitrogen, Inc., USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2019Quote: ... Both HEK293 and HEK293T cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco/Invitrogen, Inc., USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2020Quote: Plasmids encoding RiboTag constructs were used to transiently transfect HEK293 cells with lipofectamine 3000 (Thermo Fisher Scientific) as recommended by the manufacturer ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum (FBS; Invitrogen) and 200 U/ml of penicillin and streptomycin (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: HEK293 cells were grown and maintained in DMEM/F12 medium (Gibco/Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% FCS (Eurobio ...
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bioRxiv - Cell Biology 2021Quote: Cells including MEFs and STIM1-KO HEK293 cells (Emrich et al., 2019) were maintained in DMEM (Invitrogen) supplemented with 10% FBS and 1% Penicillin/Streptomycin (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: HEK293 FT cells were transfected with dVPR and VSVG packaging plasmids using Lipofectamine 2000 reagent (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... TAP cell lines were generated based on the HEK293 Flp-In T-REx system (Thermo Fisher Scientific). HEK293 cell lines with tetracycline inducible TAP-tagged baits were generated by co-transfection of 0.1 µg of plasmid containing the TAP-tagged bait (hsINTS13ΔC ...
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bioRxiv - Systems Biology 2023Quote: ... We cultured the cells in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen; RAW 264.7, HEK293, and NIH-3T3) or F-12K medium (Caisson ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells were seeded in 10 cm2 dish with Dulbecco’s Modified Eagle Medium (#11965118, Thermo Fisher Scientific) supplemented with 25 mM HEPES ...
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bioRxiv - Bioengineering 2023Quote: ... HEK293 6E cells were used for transient production of these proteins following standard protocols for 293Fectin (Invitrogen). The cell cultures were harvested at 5 d post-transfection by centrifugation at 6,000 rpm for 15 min ...
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bioRxiv - Molecular Biology 2023Quote: ... GFP-APEX2-NIK3 expressing U2OS (U2OS:GFP-APEX2-NIK3) and HEK293 cells were cultured in Dulbecco’s modified eagle’s medium (DMEM, Gibco) with 10% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2023Quote: Flp-In™ T-REx™ 293 cells (referred to as HEK293 through the manuscript; Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 1.5ug of each plasmid was transfected independently into HEK293 cells using lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA). Forty-eight hours after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... and HEK293 (NT and FASTKD5 sgRNAs) samples were purified using the Dynabeads mRNA purification kit (ThermoFisher, 61006) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... HeLa-FRT UBAP2L KO and HEK293 cell lines were cultured in DMEM GlutaMax media (Thermo Fisher Scientific) at 37C in a humidified incubator with 5%CO2 ...
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bioRxiv - Developmental Biology 2023Quote: HEK293 cells were transfected with 3 μg of linearised plasmid using Lipofectamine 3000 Transfection Reagent (Invitrogen, #L3000001) in wells of a 6-well plate ...
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bioRxiv - Molecular Biology 2024Quote: HEK293 LgBit cells (gift from Itzhaki lab) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, ThermoFisher, 41966052) supplemented with L-glutamine (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... cells were treated with antibodies (100 or 10 nM) for 1 h at 4°C and then stained Alexa647-conjugated goat anti-human IgG (Invitrogen, A21445) for 0.5 h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... we use 1µg/ml final concentration of ethidium bromide to stain parasitized erythrocytes and 4 µl/ml Alexa Fluor 488-conjugated goat anti-human IgG (H+L) (Invitrogen, Life Technologies), based on optimal signal to noise between positive control plasma (10 Mali highly exposed individual plasma ...
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bioRxiv - Cancer Biology 2022Quote: ... We collected viral supernatants after 48 h and used them to infect the Jurkat human cell line by spinoculation with 4 μg ml-1 Polybrene Infection/Transfection Reagent (Fisher Scientific). We selected infected human cell lines with 1 mg ml-1 blasticidin (InvivoGen ...
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bioRxiv - Immunology 2024Quote: ... cells were incubated overnight at 4°C with rat anti-human HLA-DR (clone: YD1/63.4) monoclonal antibody (Thermo-Fisher Scientific, USA), rabbit anti-human CD16 (clone ...
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bioRxiv - Microbiology 2023Quote: ... falciparum 3D7 blood-stage cultures were maintained at 4% hematocrit in human A+ erythrocytes in Rosewell Park Memorial Institute (RPMI) 1640/Hepes medium (Gibco, 11875119) supplemented with 0.5% Albumax II (Gibco ...
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bioRxiv - Systems Biology 2023Quote: ... a human brain organoid cultured during 4 months has been dissociated in TRIzol RNA isolation reagent (Thermo Fisher Scientific ref: 15596026). Extracted RNA has been processed with the NEBNext Ultra II RNA Library Prep Kit for Illumina (E7770) ...
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bioRxiv - Immunology 2023Quote: ... ROR2 (4-1) (heavy and light chains connected by human IgG3 upper+middle Hinge) were synthesized by GeneArt (ThermoFisher, Regensburg, Germany) and subcloned into lentiviral backbones containing IgG3- or CD8α-derived spacer domains ...
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bioRxiv - Bioengineering 2023Quote: ... wells were washed 3 times with PBS with 0.1% tween (PBST) and then incubated overnight at 4 °C with a human hemoglobin-FITC conjugated antibody (Life Technologies, A80134F). Samples were washed three times with PBST prior to imaging on a Keyence BZ-X800 microscope.
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bioRxiv - Immunology 2023Quote: Cryopreserved/thawed PBMCs were stained for 30 minutes at 4°C with the following reagents or conjugated anti-human mAbs: Live/Dead Aqua (Life Technologies), anti-CD4-PerCP (L200 ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was incubated O/N at 4°C with gentle agitation with 1:2000 goat anti-human complement C3 antibody (Thermo Scientific) diluted in 1X TBS/0,5% Tween/3% BSA ...
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bioRxiv - Immunology 2024Quote: ... was used for sensitizing the cells in the presence of recombinant human IL-4 (Gibco, PeproTech, #200-04; 10 ng/mL) for three days ...
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bioRxiv - Cell Biology 2020Quote: ... Mitogenic stimulus conditions were applied immediately after irradiation and consisted of control medium with the addition of 1 ng/mL TGF-β1 and 5 ng/mL basic fibroblastic growth factor (bFGF) (PHG9204 and PHG0264; Thermo Fisher Scientific). In additional experiments with human cartilage ...
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bioRxiv - Molecular Biology 2022Quote: ... and incubating at 37°C in a 5% CO2 humidified incubator in DFGM supplemented with 5 ng mL−1 TGF-β1 (Thermo Fisher Scientific) and 100 μg mL−1 ascorbic acid ...
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bioRxiv - Cancer Biology 2024Quote: ... the titration curve was generated by successively diluting the concentration of TGF-β1 or Inhibin A PCR amplicon inserted into the TOPO cloning vector (Thermo Fisher Scientific). The sequences of primer pairs are listed in Table S1 and S2.
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bioRxiv - Pathology 2024Quote: ... immunohistochemistry was performed using one of two rabbit polyclonal primary antibodies (COX-2, Biocare, CRM-306 and TGF-B1, Invitrogen, PA1-29032) that were incubated for 30 minutes at room temperature ...